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Oil palm tissue culture breeding method

A tissue culture, oil palm technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of shortening the tissue culture time of seedlings, unable to meet large-scale planting, low oil palm proliferation and differentiation rate, etc. The effect of shortening the cultivation time, short cultivation period and high rooting rate

Inactive Publication Date: 2012-10-10
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to overcome the deficiencies in the prior art and provide a kind of reproduction method of oil palm tissue culture, use the immature embryo of oil palm as explant, obtain regenerated plant by inducing differentiation, solve the current low rate of proliferation and differentiation of oil palm, Proliferation is slow and cannot meet the problems of large-scale planting. It can effectively shorten the time for seedling tissue culture, and has strong implementability and repeatability. It has laid a good theoretical foundation for oil palm industrialization and large-scale seedling cultivation. Prerequisites for industrial development

Method used

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  • Oil palm tissue culture breeding method

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Effect test

Embodiment 1

[0017] 1. Take 80 seed fruits collected 6 to 12 weeks after the flowering stage, which are basically the same size, have semi-red skin, and have not yet developed to the hard core stage. Wash them with tap water indoors, remove the pulp, cut the seed cores, and take out the ones with embryos. Endosperm pieces, first soaked in 70% alcohol for a few seconds, then rinsed with sterilized water 3 times, and then sterilized with saturated bleaching powder disinfectant for 10-30 minutes, the sterilized materials must be rinsed 3 times with sterile water Inoculated into callus induction medium (N 6 1 culture medium + activated carbon 3.0g / L + sucrose 30g / L + carrageenan 4.5g / L + 2,4-D 2.5mg / L), cultured in a dark room at 24°C for 80 days to obtain callus, The results are shown in Table 1. where N 6 1 Culture medium can refer to Ruslan Abdullah in the seventh issue of 2004 0717 ~ The Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies...

Embodiment 2

[0022] 1. Take 80 seed fruits collected 6 to 12 weeks after the flowering stage, which are basically the same size, have semi-red skin, and have not yet developed to the hard core stage. Wash them with tap water indoors, remove the pulp, cut the seed cores, and take out the ones with embryos. For the endosperm block, first soak it with 70% alcohol for 2-10 minutes, then rinse it with sterilized water for 3 times, and then soak it with 2% sodium hypochlorite solution for 10-20 minutes. Inoculate into callus induction medium (N 6 1 medium + activated carbon 3.0g / L + sucrose 30g / L + carrageenan 4.5g / L + 2,4-D 2.5mg / L), cultured in a dark room at 25°C for 75 days to obtain callus tissue, The results are shown in Table 1. where N 6 1 Culture medium can refer to Ruslan Abdullah in the seventh issue of 2004 0717 ~ The Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies published in Electronic Journal of Biotechnology in 3458 has the...

Embodiment 3

[0027] 1. Take 80 seed fruits collected 6 to 12 weeks after the flowering stage, which are basically the same size, have semi-red skin, and have not yet developed to the hard core stage. Wash them with tap water indoors, remove the pulp, cut the seed cores, and take out the ones with embryos. For the endosperm block, first soak it with 70% alcohol for a few seconds, then rinse it with sterilized water for 3 times, and then soak it with 2% sodium hypochlorite solution for 10-20 minutes. Inoculated into callus induction medium (N 6 1 medium + activated carbon 3.0g / L + sucrose 30g / L + carrageenan 4.5g / L + 2,4-D 2.5mg / L), cultured in a dark room at a temperature of 27°C for 65 days to obtain callus tissue, The results are shown in Table 1. where N 6 1 Culture medium can refer to Ruslan Abdullah in the seventh issue of 2004 0717 ~ The Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies published in Electronic Journal of Biotechnol...

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Abstract

The invention belongs to the technical field of seedling tissue culture, and relates to an oil palm tissue culture breeding method. The method comprises the steps of: taking young oil palm embryo as explant, disinfecting and inducing callus; conducting adventitious bud culture to the callus, and later conducting multiplication; conducting rooting culture to the multiplicated adventitious buds; and exercising rooted seedling, and transplanting into disinfected culture medium for culturing, thus obtaining oil palm regeneration plant. By taking young oil palm embryo as explant and obtaining regenerated plant through induced differentiation, the method solves the problems that the current oil palm has low multiplication differentiation rate, slow multiplication and incapability of meeting large-scale planting, effectively shortens the seedling tissue culture time, and has the characteristics of being simple in process, short in culture period, high in rooting rate, high in transplantation survival rate, and the like, thus establishing a good basis for industrialized and large-scale oil palm seedling, and providing precedent conditions for the oil palm industrialization development.

Description

technical field [0001] The invention belongs to the technical field of seedling tissue culture, and relates to a propagation method of oil palm tissue culture. Background technique [0002] Oil palm is a perennial monocotyledonous plant belonging to the genus Oil Palm of the family Palmaceae. It is an important tropical woody oil crop. Oil palm in my country is mainly distributed in Hainan, southern Guangdong, southern Taiwan, southern Yunnan and other regions. Oil palm is the oil-producing plant with the highest production efficiency in the world. The oil production is 2-3 times that of coconut, 7-8 times that of peanut, and 9-10 times that of soybean, which is far higher than other oil crops, so it is well-known For the "world oil king". Palm oil is an important part of the world's oil market. At present, it accounts for more than 30% of the world's total oil production. It is widely used in the food industry and chemical industry, and has become the most competitive raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 沈雁杨耀东周焕起陈卫军马子龙
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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