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Method for producing L-arabinose

A kind of technology of arabinose and production method, which is applied in the production field of L-arabinose, can solve the problems of high efficiency, inability to directly produce xylose, inability to purify, etc.

Active Publication Date: 2010-03-10
SHENGQUAN HEALTANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation and deficiency that this patent exists are: 1) can only produce arabinose and xylitol with corncob, and raw material source is single
2) Before the fermentation, the fermentation inhibitors such as benzene rings, heterocycles and other polymers and metal ions were not removed from the feed liquid, so that the fermentation could not be carried out smoothly; 3) The patent was used to separate arabinose and xylose There is no step to remove glucose and galactose before the alcohol, so that the chromatographic separation of arabinose and xylitol cannot be purified
5) This patented technology can only produce xylitol, but cannot directly produce xylose, and its application range is greatly limited
All miscellaneous sugars including xylose and xylan in this patent are fermented and removed by yeast, which brings about several problems: First, xylan cannot be fermented by yeast and must be hydrolyzed into monosaccharides before fermentation ;In addition, the main component in the hydrolyzate is xylose. If most of the xylose is removed by fermentation, bacteria that can utilize five-carbon sugar must be used, which will inevitably lead to the loss of arabinose fermentation.
And because the xylose content is high, if it is removed as an impurity, on the one hand, a large amount of xylose will be wasted, which is not economical and environmentally friendly; on the other hand, xylose will not be completely converted into carbon dioxide and water after fermentation, but will generate other metabolic intermediates Such as: glycerin, pyruvic acid, glyceraldehyde, xylulose, etc.
These impurities are not easily removed by crystallization, resulting in insufficient purity of the arabinose product
[0006] In summary, in the prior art, there is no production method with low cost, high efficiency, and large-scale industrial production of high-purity L-arabinose

Method used

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  • Method for producing L-arabinose

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0188] Example 1 The specific process parameters for the arabinose fraction to use Saccharomyces cerevisiae to ferment glucose and galactose to produce ethanol under anaerobic conditions are as follows: the percentages are mass percentages.

[0189] (1) first adjust the sugar concentration of the arabinose solution to 10-40%, preferably 20-35%;

[0190] (2) The composition of the culture medium nutrient salt is: urea or ammonium sulfate: 0.01~0.5%, preferably 0.05~0.2%; Potassium dihydrogen phosphate: 0.01~0.5%, preferably 0.01~0.2%; Magnesium sulfate: 0.01~0.2% 0.4%, preferably 0.01-0.2%;

[0191] (3) The parameters of the fermentation process are: pH: 2.5~5, temperature 33~45°C, no air or a small amount of air (0.1~0.3vvm) at the beginning of fermentation, when the cell concentration reaches 10 8 / ml, the air was stopped and anaerobic fermentation was carried out.

[0192] (4) Technical indicators: glucose removal rate 97.15-98.5%, ethanol yield 98-99%, arabinose yield 9...

example 2

[0193] Example 2, The specific process parameters of the arabinose fraction using Saccharomyces cerevisiae to ferment under aerobic conditions to remove glucose and galactose to generate ethanol, carbon dioxide and water are as follows: the percentages are mass percentages.

[0194] (1) first adjust the sugar concentration of the arabinose solution to 8-30%, preferably 10-25%;

[0195] (2) The composition of the culture medium nutrient salt is: ammonium sulfate: 0.1~3.5%, preferably 0.5~2.5%; potassium dihydrogen phosphate: 0.1~5%, preferably 0.5-3%; magnesium sulfate: 0.05~4% , preferably 0.2-2%; corn steep liquor (on dry matter basis): 0.5-20%, preferably 1-10%.

[0196] (3) The parameters of the fermentation process are: temperature 25-45° C., preferably 30-40; pH: 3.5-5.5, air volume 0.2-0.5 vvm.

[0197] After 10-28 hours of fermentation, the removal rate of glucose is 95%-98%, the removal rate of galactose is 50%-70%, the yield of ethanol is 90-95%, and the yield of a...

example 3

[0198] Example 3, The arabinose fraction uses shikimic acid-deficient Bacillus subtilis to ferment under aerobic conditions to remove glucose and galactose to generate D-ribose. The specific process parameters are as follows: the percentages are mass percentages.

[0199] (1) Medium and culture conditions:

[0200] a. Incline medium: glucose 0.5-2%, preferably 0.8-1.6%; peptone 0.4-2%, preferably 0.8-1.5%; yeast extract 0.1-1%, preferably 0.2-0.6%; sodium chloride 0.1-1.2% , preferably 0.2-0.8%; agar 0.8-3%, preferably 1.5-2.5%; pH 6.0-8.0, preferably 6.8-7.5; culture temperature 30-40°C, preferably 32-38°C; culture time 12-36 hours, Preferably 18-24h.

[0201] B, seed culture medium: glucose 1~3%, preferably 1.5~2.2%; Corn steep liquor (in dry matter) 1~3.5%, preferably 1.5~2.5%; Yeast extract 0.1~1%, preferably 0.2~0.6%; Dipotassium hydrogen phosphate 0.1-1%, preferably 0.2-0.7%; potassium dihydrogen phosphate 0.1-0.8%, preferably 0.1-0.5%; pH 6.0-8.0, preferably 6.8-7.5...

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Abstract

The invention relates to a method for producing L-arabinose, which can simultaneously produce ethanol, D-ribose and citric acid as byproducts. The method utilizes a hydrolyzate of agricultural waste, a xylose mother liquor and / or a production waste liquor containing pentose as the raw materials and comprises the following steps: (a) pretreating the raw materials; (b) carrying out the chromatographic resolution to separate the raw materials into an arabinose-stage liquor and a xylose-stage liquor; (c) removing impurities and unwanted bacteria, which have an inhibiting effect on fermentation, as well as unwanted saccharides at least comprising glucose and galactose; (d) and carrying out the post treatment on the arabinose-stage liquor to obtain the L-arabinose. Steps (c1) and (c2) are arranged before the chromatographic resolution in step (b) or after step (b). The invention has the advantages of low production cost and high efficiency, and is suitable for large-scale industrial production.

Description

technical field [0001] The present invention relates to the production technology of L-arabinose, especially using hemicellulose hydrolyzate, xylose mother liquor and production waste liquid containing five-carbon sugar as raw materials to produce L-arabinose products, and simultaneously by-product ethanol, D-ribose, Methods for glucose fermentation products such as citric acid. technical background [0002] L-arabinose belongs to the five-carbon aldose, and exists in the form of arabinan, arabinoxylan, arabinose galactose and hemifiber similar to higher plants. L-arabinose is a sweetener without calories, and can also be used as a pharmaceutical intermediate, for the preparation of bacterial culture media in the biochemical field, and for the synthesis of spices, etc. Xylose products such as xylose and xylitol are more and more popular as food sweeteners. Many health care functions of xylose and xylitol have been confirmed by scientific research, such as lowering blood su...

Claims

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Application Information

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IPC IPC(8): C07H3/02C07H1/06C07H1/08C13K13/00B01D15/08C12P7/06C12P19/02C12P7/48C12R1/125C12R1/685C12R1/865C12R1/78C12R1/645
CPCY02E50/17Y02E50/10
Inventor 唐一林江成真高绍丰崔建丽贺芹孟海波
Owner SHENGQUAN HEALTANG
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