The invention relates to the technical field of metallurgic mineral separation and provides a mixed flocculating agent capable of accelerating settling of superfine grain tailing flocculation and application thereof. According to the mixed flocculating agent, firstly from 13 million to 14 million, by molecular weight, of polyacrylamide is prepared into a water solution with mass faction from 0.05% to 0.3%, then the water solution is mixed uniformly with quartz sand from 20 to 40 grits or iron rough concentrate according to the mass ratio ranging from 0.3:1 to 6:1, and the flocculating agent is obtained; by means of the mixed flocculating agent, the settling time and the settling efficiency of superfine grain tailing can be accelerated, the water quality of separating medium is improved obviously, so that the separating medium generated in the flocculation process can be recycled and reused as mineral separation used water, and the flocculating agent usage is greatly reduced (by about from four to ten grams per ton of dry ore); meanwhile, a novel superfine grain tailing flocculation settling technology is provided, the superfine grain tailing flocculation settling time is greatly shortened, the production efficiency is enhanced, the problem that water content stays at a high level after the superfine grain tailing concentrates is solved, a new process route is provided for the superfine grain tailing dry pile technology, and prominent contributions are made to environment protection and comprehensive utilization of resources.