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Method for rapidly screening silicate bacteria

A silicate bacteria, a fast technology, applied in the direction of bacteria, etc., can solve the problems of acid-producing dominant silicate bacteria without intuitive visibility, and few types of bacteria in primary screening conditions.

Inactive Publication Date: 2014-10-22
ZHENGZHOU MINERALS COMPOSITIVE UTILIZATION RES INST CHINESE GEOLOGICAL ACAD +1
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Problems solved by technology

In the traditional method, the primary screening conditions are harsh, and the strains obtained are few, and there is no intuitive visibility for acid-producing silicate bacteria in the secondary screening.

Method used

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  • Method for rapidly screening silicate bacteria

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Embodiment 1

[0022] (1) Bauxite mining area (bauxite can be collected from Changzhai Town, Changshun County, Qiannan Buyi and Miao Autonomous Prefecture, Guizhou Province, Yunbiao Town, Heng County, Nanning City, Guangxi Zhuang Autonomous Region, or Menggong Township, Guigang City, Guangxi) 0-20cm of soil was used as a sample and stored in a refrigerator at 4°C. Weigh 10g of the soil sample to be tested, add it to 100ml of sterile water, shake it with a quick mixer for 5min, let it stand for 1h, take 3ml of the supernatant and add it to the liquid nitrogen-free medium of 200mg / L illite, put it in The cells were enriched and cultured at 180 rpm on a constant temperature shaker at 30°C for 3 days. After enrichment culture, transfer 10% volume of the first culture solution into 200mg / L illite liquid medium, and adopt the method of gradually increasing the concentration of bauxite to enrich and culture silicate bacteria; 200 mg / L of illite concentration, so that the final illite concentration...

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Abstract

A method for rapidly screening silicate bacteria comprises the following steps: (1) obtaining a soil bacterium suspension; (2) performing enrichment culture, namely employing a method of gradually improving the addition amount of bauxite to perform enrichment culture on silicate bacteria; (3) performing screening culture, namely selecting a mucus-shaped raised transparent bacterium colony on a culture medium plate according to bacterium colony growing time and bacterium colony form, and performing once-more culture until a pure culture is obtained; and (4) performing secondary screening. By employing the method of gradually improving the addition amount of bauxite, bacterial relatively easily adapt to silicate environment, and thus more silicate bacteria are enriched. By employing the method of smearing CaCO3 powder on plate surface for enabling CaCO3 to not be precipitated to the bottom of the plate, screening of acid-producing bacteria is visualized. By replacing solid FeCl3 added into the culture medium in a trace amount with a FeCl3 solution, the FeCl3 solution is relatively easy to utilize by bacteria. The method is capable of rapidly conveniently screening and domesticating silicate bacteria in a laboratory.

Description

technical field [0001] The invention relates to a screening method for silicate bacteria, in particular to a method for rapidly screening silicate bacteria in a laboratory. Background technique [0002] With the massive exploitation of mineral resources, high-grade and easy-to-select mineral resources are decreasing day by day. At the same time, environmental protection regulations are becoming more and more stringent. Some traditional development methods of mineral resources are no longer suitable for low-grade mineral resources. Biological mineral processing has shown a good application prospect in mineral processing due to the advantages of low ore grade requirements, low cost, low energy consumption, simple production process and environmental friendliness. Biological mineral processing is a technology that removes impurities from ores or recovers valuable metals through the interaction of microorganisms or their metabolites with minerals. The process of using microorgan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 赵恒勤贺治国张耀胡四春王龙
Owner ZHENGZHOU MINERALS COMPOSITIVE UTILIZATION RES INST CHINESE GEOLOGICAL ACAD
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