Methods for preparing L-tagatose

A technology for tagatose and glucose, applied in the field of preparing L-tagatose, can solve the problems of high price, unrealistic L-tagatose, regeneration of coenzyme factors, etc., and achieves the effect of high conversion efficiency

Inactive Publication Date: 2010-04-07
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The raw material L-biloxose used in this method itself is also a very rare and rare sugar, which is expensive, and it has to go through the process of redu

Method used

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  • Methods for preparing L-tagatose
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  • Methods for preparing L-tagatose

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Acetobacillus suboxydis was cultured in a fermentation medium containing 1% D-galactitol

[0051] Acetobacter suboxydans was cultured in a seed medium at 30°C and 250 rpm for 48 hours to obtain a seed bacterial solution. Each liter of seed medium contains: 2 grams of D-galactitol (also called dulcitol or sweet alcohol); 5 grams of sorbitol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium hydrogen phosphate; 5 grams of calcium carbonate , Add water to 1 liter, pH 6.0. Then, 10 ml of the seed liquid was inoculated into 90 ml of fermentation medium, and cultured at 32° C. and 300 rpm for 24 hours. The composition of each liter of fermentation medium contains: 10 grams of D-galactitol; 1 gram of sorbitol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium phosphate; 5 grams of calcium carbonate. Add water to 1 liter. pH6.0. After the cultivation, the conversion rate and tagatose content were analyzed by HPLC, and the results were as ...

Embodiment 2

[0053] Acetobacillus suboxydis was cultured in a fermentation medium containing 4% D-galactitol

[0054] Acetobacter suboxydans was cultivated in a seed culture medium at 30° C. and 250 rpm for 48 hours to obtain a seed bacterial solution. Each liter of seed medium contains: 4 grams of D-galactitol; 2 grams of glycerol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium hydrogen phosphate; 10 grams of calcium carbonate. Add water to 1 liter, pH 6.0 . Then 10 ml of the seed liquid was inoculated into 90 ml of fermentation medium, and cultured at 32° C. and 300 rpm for 24 hours. The composition of each liter of fermentation medium contains: 40 grams of D-galactitol; 2 grams of sorbitol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium hydrogen phosphate; 5 grams of calcium carbonate. Add water to 1 liter. pH6.0. After the cultivation, the conversion rate and the content of tagatose were analyzed by HPLC. After integration calculation, the ...

Embodiment 3

[0056] Acetobacter suboxydis was cultured in a fermentation medium containing 10% D-galactitol

[0057] Acetobacter suboxydis was cultured in a seed culture medium at 25° C. and 250 rpm for 48 hours to obtain a seed bacterial solution. Each liter of seed culture medium contains: 4 grams of D-galactitol; 2 grams of xylitol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium hydrogen phosphate; 10 grams of calcium carbonate, add water to 1 liter, pH 6 .0. Then 10 ml of the seed liquid was inoculated into 90 ml of fermentation medium, and cultured at 32° C. and 300 rpm for 48 hours. The composition of each liter of fermentation medium contains: 100 grams of D-galactitol; 5 grams of xylitol; 10 grams of glucose; 10 grams of yeast powder; 2 grams of dipotassium hydrogen phosphate; 5 grams of calcium carbonate, add water to 1 liter , PH6.0. After the cultivation, the conversion rate and the content of tagatose were analyzed by HPLC. After integration calculation, ...

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Abstract

The invention relates to methods for preparing L-tagatose, which belongs to the field of biotechnology. A method for preparing the L-tagatose comprises the following steps: preparing a culture medium; culturing acid-producing bacteria to obtain primary seed liquid; obtaining secondary seed liquid; inoculating secondary seed liquid to a fermentation culture medium to obtain fermentation liquor; obtaining primary yeast seed liquid and secondary yeast seed liquid and centrifuging the primary yeast seed liquid and the secondary yeast seed liquid to obtain yeast cells; culturing the yeast cells to obtain clear fermentation liquor; concentrating, decolorizing and filtering the clear fermentation liquor to obtain solution; and passing the solution through an ion exchange resin, performing crystallization, washing crystals and drying the crystals to obtain the L-tagatose. A method for preparing the L-tagatose by converting D-dulcitol by using resting cells comprises the following steps: preparing a culture medium; culturing bacteria; suspending somatic cells of the bacteria to obtain solution; inoculating candida ssp. CGMCC No.3268 to obtain fermentation liquor; and subjecting the fermentation liquor to decolorizing and desalting processing to obtain aqueous solution, concentrating the aqueous, performing crystallization, washing crystals and drying the crystals to obtain the L-tagatose. The methods of the invention can be used for preparing pharmaceutical L-tagatose.

Description

Technical field [0001] The invention relates to a preparation method in the field of biotechnology, in particular to a method for preparing L-tagatose. Background technique [0002] Tagatose is a rare six-carbon sugar, divided into D-tagatose and L-tagatose. Although they exist in nature, the amount is very scarce, and the amount of L-tagatose is very rare. D-tagatose, as a low-calorie and safe sugar, was approved by the US FDA as early as 2001, and there are unlimited restrictions. It has also entered the phase III clinical trial as a clinical drug for preventing diabetes. [0003] After searching the literature of the prior art, it is found that the main methods for synthesizing D-tagatose are: using metal hydroxide or sodium aluminate as a catalyst to isomerize D-galactose to produce D-tagatose (US Patent US5002612, publication date: March 26, 1991; Chinese patent CN200610123316.5, publication date: 2007.6.27; World of Chemistry, 2009, Issue 3, pages 187-188); L-arabinose isome...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/02C12R1/01C12R1/72
Inventor 程海荣邓子新
Owner SHANGHAI JIAO TONG UNIV
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