Fermented biological feed and preparation method thereof
A technology for fermenting organisms and feed, which is applied in the field of fermented biological feed and its preparation to achieve the effects of reducing usage, improving odor pollution and reducing excretion
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Embodiment 1
[0048] A fermented biological feed is prepared by the following method:
[0049] (1) Bacterial strain cultivation: Bacillus subtilis, baker's yeast, enterococcus faecalis, plant lactobacillus, respectively inoculate to glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus is water, and pH is 7.0 In the culture medium, Bacillus subtilis and baker's yeast were cultured at 35°C and 200 rpm for 16h; Enterococcus faecalis and Pediococcus lactis were cultured statically at 35°C for 16h to obtain a Bacillus subtilis bacterial liquid (the number of viable bacteria was 1.4×10 8 cfu / mL), baker's yeast liquid (3.0×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 2.3×10 8 cfu / mL), Lactobacillus plantarum solution (the number of viable bacteria is 3.0×10 8 cfu / mL), the above bacterial liquid was inoculated into 2wt‰ brown sugar, 2wt‰ yeast extract, the balance was water, and the pH was 7.0, cultured at 28°C for 8 hours to obtain a mixed bacterial liq...
Embodiment 2
[0054] A fermented biological feed is prepared by the following method:
[0055] (1) Strain cultivation: Bacillus natto, Candida utilis, Enterococcus faecalis, and Pediococcus lactis were respectively inoculated into glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, and the balance was water, In the culture medium with pH 7.0, Bacillus natto and Candida utilis were cultured at 36°C and 200 rpm for 16h; The number of live bacteria is 2.1×10 8 cfu / mL), Candida utilis bacteria liquid (the number of viable bacteria is 2.8×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 1.6×10 8 cfu / mL), Pediococcus lactis bacteria solution (the number of viable bacteria is 3.5×10 8 cfu / mL), and then inoculate the above-mentioned bacterial solution into 2wt‰ brown sugar, 2wt‰ yeast extract, the balance being water, and culture medium with a pH of 7.0, and cultivate at 30°C for 6 hours to obtain a mixed bacterial solution; wherein Bacillus natto The inoculum vo...
Embodiment 3
[0060] A fermented biological feed is prepared by the following method:
[0061] (1) Strain cultivation: Bacillus subtilis, Bacillus licheniformis, Candida utilis, Enterococcus faecalis, Pediococcus lactis were respectively inoculated into glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus In a medium with water and a pH of 7.0, Bacillus subtilis, Bacillus licheniformis, and Candida utilis were cultured at 36°C and 200 rpm for 16 hours; Enterococcus faecalis and Pediococcus lactis were cultured statically at 36°C for 16 hours to obtain subtilis Bacillus bacteria liquid (the number of live bacteria is 1.5×10 8 cfu / mL), Bacillus licheniformis bacteria solution (the number of viable bacteria is 1.0×10 8 cfu / mL), Candida utilis bacteria liquid (the number of viable bacteria is 3.0×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 2.0×10 8 cfu / mL), lactic acid bacteria solution (the number of viable bacteria is 3.2×10 8 cfu / mL), and then ...
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