Fermented biological feed and preparation method thereof

A technology for fermenting organisms and feed, which is applied in the field of fermented biological feed and its preparation to achieve the effects of reducing usage, improving odor pollution and reducing excretion

Inactive Publication Date: 2017-01-04
湖北凌卓生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing strains used to ferment and produce biological feed all focus on the growth performance and acid product

Method used

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  • Fermented biological feed and preparation method thereof
  • Fermented biological feed and preparation method thereof
  • Fermented biological feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A fermented biological feed is prepared by the following method:

[0049] (1) Bacterial strain cultivation: Bacillus subtilis, baker's yeast, enterococcus faecalis, plant lactobacillus, respectively inoculate to glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus is water, and pH is 7.0 In the culture medium, Bacillus subtilis and baker's yeast were cultured at 35°C and 200 rpm for 16h; Enterococcus faecalis and Pediococcus lactis were cultured statically at 35°C for 16h to obtain a Bacillus subtilis bacterial liquid (the number of viable bacteria was 1.4×10 8 cfu / mL), baker's yeast liquid (3.0×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 2.3×10 8 cfu / mL), Lactobacillus plantarum solution (the number of viable bacteria is 3.0×10 8 cfu / mL), the above bacterial liquid was inoculated into 2wt‰ brown sugar, 2wt‰ yeast extract, the balance was water, and the pH was 7.0, cultured at 28°C for 8 hours to obtain a mixed bacterial liq...

Embodiment 2

[0054] A fermented biological feed is prepared by the following method:

[0055] (1) Strain cultivation: Bacillus natto, Candida utilis, Enterococcus faecalis, and Pediococcus lactis were respectively inoculated into glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, and the balance was water, In the culture medium with pH 7.0, Bacillus natto and Candida utilis were cultured at 36°C and 200 rpm for 16h; The number of live bacteria is 2.1×10 8 cfu / mL), Candida utilis bacteria liquid (the number of viable bacteria is 2.8×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 1.6×10 8 cfu / mL), Pediococcus lactis bacteria solution (the number of viable bacteria is 3.5×10 8 cfu / mL), and then inoculate the above-mentioned bacterial solution into 2wt‰ brown sugar, 2wt‰ yeast extract, the balance being water, and culture medium with a pH of 7.0, and cultivate at 30°C for 6 hours to obtain a mixed bacterial solution; wherein Bacillus natto The inoculum vo...

Embodiment 3

[0060] A fermented biological feed is prepared by the following method:

[0061] (1) Strain cultivation: Bacillus subtilis, Bacillus licheniformis, Candida utilis, Enterococcus faecalis, Pediococcus lactis were respectively inoculated into glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus In a medium with water and a pH of 7.0, Bacillus subtilis, Bacillus licheniformis, and Candida utilis were cultured at 36°C and 200 rpm for 16 hours; Enterococcus faecalis and Pediococcus lactis were cultured statically at 36°C for 16 hours to obtain subtilis Bacillus bacteria liquid (the number of live bacteria is 1.5×10 8 cfu / mL), Bacillus licheniformis bacteria solution (the number of viable bacteria is 1.0×10 8 cfu / mL), Candida utilis bacteria liquid (the number of viable bacteria is 3.0×10 8 cfu / mL), Enterococcus faecalis bacteria liquid (the number of viable bacteria is 2.0×10 8 cfu / mL), lactic acid bacteria solution (the number of viable bacteria is 3.2×10 8 cfu / mL), and then ...

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Abstract

The invention belongs to the field of feed production, and particularly relates to a fermented biological feed and a preparation method thereof. The preparation method of the fermented biological feed comprises the following steps: respectively carrying out strain rejuvenation activation and primary propagation on amylase-producing bacteria, proteinase-producing bacteria and acid-producing bacteria to obtain an amylase-producing bacteria solution, a proteinase-producing bacteria solution and an acid-producing bacteria solution; proportionally inoculating the amylase-producing bacteria solution, proteinase-producing bacteria solution and acid-producing bacteria solution into a co-culture medium, and carrying out co-culture to obtain a mixed bacteria solution; and adding the mixed bacteria solution and water into a fermentation raw material, uniformly mixing, carrying out trough fermentation, and obtaining the fermented biological feed after the fermentation finishes. The fermented biological feed is partially substituted for the traditional animal feed or animal feed additive, is capable of effectively enhancing the feed nutritive value and utilization ratio, improving the problem in intestinal tracts in livestock/poultry breeding, enhancing the disease resistance of the bred animals, and reduces the use of antibiotic drugs.

Description

technical field [0001] The invention belongs to the field of feed production, and in particular relates to a fermented biological feed and a preparation method thereof. Background technique [0002] At present, in livestock and poultry breeding, there are generally problems of unhealthy intestines of livestock and poultry and poor intestinal nutrient absorption. Among the existing feeds, 80% of the feeds have poor utilization, and more than 50% of the feeds eaten by livestock and poultry will be damaged. Because the intestinal tract cannot absorb it, it is converted into feces and excreted; at the same time, livestock and poultry diseases occur frequently and medication is used frequently. There is a phenomenon of antibiotic abuse, and the cost of breeding also increases. With the introduction of fermented biological feed, fermented feed with beneficial bacteria is considered to be a better choice to solve the above problems. However, the existing strains used for fermentat...

Claims

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Application Information

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IPC IPC(8): A23K10/18
Inventor 姜涛徐凯胡晓光
Owner 湖北凌卓生物工程有限公司
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