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Method for anaerobic synthesis of volatile fatty acid by using wood carbon source sewage mixed bacteria

A technology of volatile fatty acids and mixed strains, which is applied in the field of anaerobic synthesis of volatile fatty acids by mixed strains of woody carbon source domesticated sewage, can solve the problems of high cost, single type of acid production, complex preparation process, etc. Effect of improving utilization rate, reducing energy consumption and cost

Inactive Publication Date: 2019-04-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there have been many studies on the production of lactic acid by fermentation of lignocellulose (corn stalks, corn cobs, rice straw, etc.), but the types of acid produced are single, and when cellulose is used as raw material, it needs to be extracted, the preparation process is complicated, and the cost is relatively high. high

Method used

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  • Method for anaerobic synthesis of volatile fatty acid by using wood carbon source sewage mixed bacteria

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Experimental program
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Effect test

Embodiment 1

[0056] Add domesticated sewage into a 500mL container. The initial mixed liquid suspended particle concentration (MLSS) of the sewage is 2000mg / L. L, the phosphorus source concentration is 30mg / L, and every 1L of influent water includes 100mg / L MgSO 4 and 1mg / L trace element solution, nitrogen and deoxygenation for 5 minutes in the process of fermentation acid production, the pH value is 6, the fermentation temperature is 25 ° C, and the fermentation time is 12 hours. During acid production, the pH was adjusted to the initial value every 2 hours. After acid production, the acidified wastewater was sampled and measured, the mixed solution was centrifuged and filtered, and the total amount of volatile fatty acids was determined by colorimetry. Then adjust the pH value of the filtrate to 2-3, and use gas chromatography to measure the composition and content of volatile fatty acids.

Embodiment 2

[0058] Add domesticated sewage into a 500mL container. The initial mixed liquid suspended particle concentration (MLSS) of the sewage is 2000mg / L. L, the phosphorus source concentration is 30mg / L, and every 1L of influent water includes 100mg / L MgSO 4 and 1mg / L trace element solution, nitrogen and deoxygenation for 5 minutes during the fermentation process of acid production, the pH value is 6, the fermentation temperature is 30°C, and the fermentation time is 12 hours. During acid production, the pH was adjusted to the initial value every 2 hours. After acid production, the acidified wastewater was sampled and measured, the mixed solution was centrifuged and filtered, and the total amount of volatile fatty acids was determined by colorimetry. Then adjust the pH value of the filtrate to 2-3, and use gas chromatography to measure the composition and content of volatile fatty acids.

Embodiment 3

[0060] Add domesticated sewage into a 500mL container. The initial mixed liquid suspended particle concentration (MLSS) of the sewage is 2000mg / L. L, the phosphorus source concentration is 30mg / L, and every 1L of influent water includes 100mg / L MgSO 4 and 1 mg / L trace element solution, nitrogen and deoxygenation for 5 minutes during the fermentation process, the pH value is 6, the fermentation temperature is 35°C, and the fermentation time is 12 hours. During acid production, the pH was adjusted to the initial value every 2 hours. After acid production, the acidified wastewater was sampled and measured, the mixed solution was centrifuged and filtered, and the total amount of volatile fatty acids was determined by colorimetry. Then adjust the pH value of the filtrate to 2-3, and use gas chromatography to measure the composition and content of volatile fatty acids.

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Abstract

The invention discloses a method for anaerobic synthesis of volatile fatty acid by using wood carbon source sewage mixed bacteria. The method comprises the following steps of (1) performing hydrolyzing and enzymolysis on a xyloid biological resource by a high-temperature high-pressure hydrothermal pretreatment method; (2) using wood hydrolysis-enzymatic hydrolysate (reducing sugar) as a carbon source, performing anaerobic domesticizing on the sewage containing activated sludge, and performing enriching to synthetize acid-producing bacteria of volatile fatty acid; and (3) performing anaerobic fermentation on domesticized sewage to generate acid, through regulating factors of the concentration of the reducing sugar, pH value, fermentation temperature, the number of fermentation days and thelike, increasing the yield of the volatile fatty acid, finally, determining the volatile fatty acid as mixed acid of acetic acid, propanoic acid and butyric acid. Through contrast of total amount of the volatile fatty acid and the proportion of the components namely acid in various experiment groups, the optimal acid generation condition can be obtained. By the method, the purpose of synthetizingthe volatile fatty acid with low-price carbon sources can be realized, the best acid generating time is greatly shortened, and the energy consumption and the cost can be saved.

Description

technical field [0001] The invention belongs to the fields of high-efficiency and high-value utilization of biomass resources and solid waste treatment, and relates to a method for anaerobically synthesizing volatile fatty acids with woody carbon source domesticated sewage mixed strains. Background technique [0002] The increasing development of the economy and society has put forward new requirements for resources and the environment. As a renewable resource, woody biomass resources have a wide range of sources, including agricultural straw wastes, forest trees and forest product processing wastes, etc. At present, my country's woody biomass resources The utilization rate and recovery rate of biomass waste are very low, and most of them are disposed of by open burning or landfill, which is a serious waste of biomass resources. Therefore, their resource utilization has been paid more and more attention by people. Using lignocellulosic biomass in agricultural and forestry wa...

Claims

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Application Information

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IPC IPC(8): C12P7/52C12P7/54G01N30/02C12R1/365C12R1/01C12R1/42C12R1/185C12R1/38C12R1/07C12R1/645
CPCC12P7/52C12P7/54C12P2201/00C12P2203/00G01N30/02
Inventor 马晓军李冬娜尹芬
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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