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Biological seed coating for controlling soybean cyst nematodes

A technology of soybean cyst nematodes and biological species, which is applied in the direction of biocides, nematicides, chemicals for biological control, etc., and can solve the problems of less raw materials, fewer varieties of insecticides and pesticides, and weak seed coating agents

Inactive Publication Date: 2012-01-11
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, my country's seed coating agent market is relatively weak, with a single variety and few original drugs
Most of the existing seed coating agents are in the form of pesticides, and the high-toxic pesticide carbofuran is the main drug in the seed coating agent to prevent and control underground pests, and there are few new insecticidal pesticides.

Method used

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  • Biological seed coating for controlling soybean cyst nematodes
  • Biological seed coating for controlling soybean cyst nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the fermentation of biocontrol bacteria

[0020] Streptomyces venezuelae (Streptomyces venezuelae) Snea253 was inoculated into a 50L-100L fermenter for fermentation, cultured at 28° C., with an initial pH of 6, and fermented for 120h-140h. The liquid culture strain of Bacillus megaterium (Bacillus) Sneb207 is inoculated into a 50L-100L fermenter for fermentation, cultured at 25° C., pH is 8, and fermented for 48h-72h. During the sampling period, check the activity of the fermentation liquid against the target nematodes, and determine the storage time according to the activity standard curve.

Embodiment 2

[0021] Embodiment 2: the screening of element consumption

[0022] All reagents used are of analytical grade, including potassium dihydrogen phosphate, potassium sulfate, copper sulfate, manganese sulfate, ferrous sulfate, ammonium molybdate, boric acid, etc. The various elements are mixed according to different proportions and then ground, and a binder is added to coat the soybeans, and the germination potential of soybean seeds is used as a screening index to select a combination of macroelements and microelements that promote soybean growth. Combination ratio (by weight percentage): CuSO 4 0.09%, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.18%, H 3 BO 3 0.04%, MnSO 4 0.3%, FeSO 4 0.04%, KH 2 PO 4 1.5%, K 2 SO 4 1%.

Embodiment 3

[0023] Embodiment 3: Control the biological seed coating agent preparation of soybean cyst nematode

[0024] After treating the fermented liquid of various biocontrol bacteria, compounding according to the ratio of the present invention, mixing various elements in proportion, crushing and grinding, then mixing with additives evenly, adding distilled water and stirring to form a uniform viscous liquid, wet method Grinding, oscillating and mixing evenly to prepare the biological seed coating agent of the present invention.

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Abstract

The invention relates to a biological seed coating for controlling soybean cyst nematodes. The biological seed coating comprises nematicidal activity substances from two kinds of bio-control agents, elements which are prepared by mixing according to a certain proportion and various aids. As proved by a large quantity of tests, the biological seed coating meets the requirements of a coating on physical and chemical properties; and after coating, the growth of soybeans at a seedling stage is promoted remarkably, the yield of soybeans is increased, and the control effect on soybean cyst nematode disease at the seeding stage is remarkable.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, in particular to a biological seed coating agent for controlling soybean cyst nematodes. Background technique: [0002] Soybean cyst nematode (Soybean Cyst Nematode), also known as soybean verticillium wilt and dragon seedling, is one of the most important diseases restricting soybean production in the world. The disease generally reduces the yield of soybean by 10% to 30%, and in severe cases, it can reach 70% to 90%, and even leads to complete production, which is more harmful than any single disease. The disease has spread rapidly in the main soybean producing areas and has become one of the main diseases. From a global perspective, the damage and spread of soybean cyst nematodes are increasingly aggravating. In recent years, with the continuous expansion of soybean cultivation area in my country, the distribution of soybean cyst nematode (Heterodera glycines) has become wider a...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N59/26A01N59/20A01N59/16A01N59/14A01N59/00A01P5/00A01C1/06
Inventor 陈立杰万传浩段玉玺郭宁王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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