Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application

A soybean cyst nematode and soybean technology, applied in the application, nematicide, bacteria and other directions, can solve problems such as increasing the yield of Chinese cabbage, and achieve good results in solving soybean cyst nematode disease and cyst formation

Active Publication Date: 2013-02-13
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant studies have shown that Bacillus simplex has growth-promoting and biocontrol effects, and its control rate for Chinese cabbage soft rot is as high as 83%, while increasing the yield of Chinese cabbage by 50%, while the control of Bacillus simplex for soybean cyst nematode is still Not yet reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 : Bacillus simplex ( Bacillus simplex ) culture of Sneb545

[0021] First, for Bacillus simplex ( Bacillus simplex ) Sneb545 strain was fermented, and then the soybean-inducing activity test was carried out on the metabolites after fermentation to determine their inducing effect on soybean.

[0022] Bacillus simplex ( Bacillus simplex ) Sneb545 bacteria were inoculated on the culture medium, and the formula of the culture medium was beef extract-peptone medium, that is, the ingredients: 3g of beef extract, 5g of peptone, 10g of sucrose, 17-20g of agar, added distilled water to 1000mL, and cultured at 25°C for 2 days.

[0023] Then inoculate the strains into a 250mL triangular flask (each bottle contains 100mL) liquid medium. The medium formula is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3% -0.4%, 1%-2% sucrose, the rest is distilled water, the pH is 6.8-7.0, the fermentation temperature is 25-28°C, the shak...

Embodiment 2

[0024] Example 2 : Bacillus simplex ( Bacillus simplex ) Fermentation of Sneb545

[0025] Basically the same as in Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25° C., pH 7.0, and fermented for 3 days.

Embodiment 3

[0026] Example 3 : Bacillus simplex ( Bacillus simplex ) Verification of soybean systemic resistance induced by Sneb545

[0027] The induced systemic resistance of strain Sneb545 to soybean cyst nematode was explored by split-root system. In the root splitting test, the challenge root system and the response root system were completely isolated from each other in space, and the Sneb545 fermentation broth in the response root system had no direct contact with soybean cyst nematodes, and the control effect of the strain on nematodes was completely dependent on its induced resistance to plants. The assay method is reliable and has been widely used in the research of system-induced resistance of biocontrol microorganisms to soil-borne diseases.

[0028] 1. Preparation of preparations for testing

[0029] The fermentation liquid of Bacillus simplex strain Sneb545 was prepared according to the aforementioned liquid fermentation culture method, and was set aside.

[0030] 2. ...

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Abstract

The invention relates to a method for culturing a Bacillus simplex Sneb545 for inducing soybean to generate soybean cyst nematode resistance after soybean seed treatment and an application of the Bacillus simplex Sneb545, and belong to the technical field of microbial pesticides. The bacterial strain Sneb545 related in the invention is preserved in the China general microbiological culture collection center in October 11th, 2012, and the preservation number is CGMCC No.6650. After the strain is subjected to fermentation cultivation through conventional liquid, the metabolic products of the strain can be used to develop a seed treating agent for treating soybean seeds, and the treated soybean seeds can induce the soybeans to generate the soybean cyst nematode resistance. The soybean seeds which is treated through the metabolic products of the Bacillus simplex Sneb545 can induce the soybeans to generate obvious resistance to the first generation of soybean cyst nematode during the seedling stage, the Bacillus simplex Sneb545 has an obvious function of inhibiting the cytocyst of the soybean cyst nematode from forming and is a potential biocontrol bacterium, and a new method is provided for preventing the soybean cyst nematode.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to a simple bacillus capable of inducing soybean resistance to soybean cyst nematodes and its application. Background technique [0002] Soybean Cyst Nematode (SCN) is caused by soybean cyst nematode ( Heterodera glycines ) infection is one of the most serious diseases of soybean production in the world. The disease has the characteristics of wide distribution, severe damage, wide host range, multiple transmission routes, and long survival time of dormant bodies (cysts). It is a soil-borne disease that is extremely difficult to control. In recent years, with the continuous expansion of soybean cultivation area in my country, the distribution of soybean cyst nematodes has become wider and wider. It mainly occurs in 14 provinces, municipalities and autonomous regions such as the Northeast and Huanghuaihai. %, in severe cases, it can reach 70% to 80%, or even no grains. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/07
Inventor 段玉玺陈立杰朱晓峰王媛媛项鹏
Owner SHENYANG AGRI UNIV
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