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Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer

A microbial fertilizer and ecological technology, applied in the field of preparation and application of preparation and application, can solve problems such as lack of prevention and control technology, and achieve the effects of improving culture efficiency, improving utilization rate and rapid start-up.

Inactive Publication Date: 2015-07-08
宁土建工(苏州)环境修复科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when choosing a method for controlling root-knot nematodes, the role of a certain method should not be emphasized one-sidedly. At present, there is still a lack of a comprehensive control technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation and application product of microbial fertilizer for soil ecological care: its components are organic matter content ≥ 40%, including photosynthetic bacteria, nitrogen-fixing bacteria, phosphorus bacteria, colloidal bacillus, Paecilomyces lilacinus, and the number of effective viable bacteria ≥ 200 million CFU / g, of which the number of Paecilomyces lilacinus ≥ 100 million CFU / g.

[0039] The preparation method of the soil ecological care microbial fertilizer product:

[0040] (1) Preparation of Paecilomyces lilacinus liquid strain: the water used is high-energy water with a molecular oscillation frequency above 3000Mhz to configure Chase solid medium, sterilized, streaked and inoculated Paecilomyces lilacinus, placed in Cultivate in a constant temperature incubator at 25-30°C until lavender powdery conidia appear; the liquid culture method is Chase's solid medium without adding agar, on a sterile operating table, use an inoculation loop to preserve it from ...

Embodiment 2

[0048] A preparation and application product of microbial bacterial fertilizer for soil ecological care: its components are organic matter content ≥ 50%, including photosynthetic bacteria, nitrogen-fixing bacteria, phosphorus bacteria, colloidal bacillus, Paecilomyces lilacinus, and the number of effective viable bacteria ≥ 200 million CFU / g, of which the number of Paecilomyces lilacinus ≥ 200 million CFU / g.

[0049] The preparation method of the soil ecological care microbial fertilizer product:

[0050] (1) Preparation of Paecilomyces lilacinus liquid strain: the water used is high-energy water with a molecular oscillation frequency above 3000Mhz to configure Chase solid medium, sterilized, streaked and inoculated Paecilomyces lilacinus, placed in Cultivate in a constant temperature incubator at 25-30°C until lavender powdery conidia appear; the liquid culture method is Chase's solid medium without adding agar, on a sterile operating table, use an inoculation loop to preserv...

Embodiment 3

[0058] A preparation and application product of microbial bacterial fertilizer for soil ecological care: its components are organic matter content ≥ 50%, including photosynthetic bacteria, nitrogen-fixing bacteria, phosphorus bacteria, colloidal bacillus, Paecilomyces lilacinus, and the number of effective viable bacteria ≥ 500 million CFU / g, of which Paecilomyces lilacinus counts 200 million CFU / g.

[0059] The preparation method of the soil ecological care microbial fertilizer product:

[0060] (1) Preparation of Paecilomyces lilacinus liquid strains: the water used is high-energy water with a molecular oscillation frequency above 3000Mhz to configure Chase solid medium, sterilized, streaked and inoculated Paecilomyces lilacinus, placed in Cultivate in a constant temperature incubator at 25-30°C until lavender powdery conidia appear; the liquid culture method is Chase's solid medium without adding agar, on a sterile operating table, use an inoculation loop to preserve it fro...

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Abstract

The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg / mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizer, in particular to the preparation and application of soil ecological care microbial fertilizer. Background technique [0002] Plant nematodes are a type of root disease caused by coelenterate nematodes, which seriously affect crop yields. For example, Meloidogyne mainly damages vegetable roots, and lateral roots and fibrous roots are most vulnerable. Nodules or root knots of different sizes are produced on the affected roots. The root knots are white and soft at first, and then become light yellowish brown or dark brown. The surface is rough and sometimes cracked. When the root knot is cut open, small white pearly particles can be seen, which are female adults of root knot nematode. Vegetables grow slowly after root-knot nematode infestation, and the leaves are small and yellow. When the disease is severe, the plants are short, weak or poorly set, and when the weather is hot and dry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05F17/00
CPCY02W30/40
Inventor 毕德王雪玲冯明凯
Owner 宁土建工(苏州)环境修复科技有限公司
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