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Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof

A soybean, stress-resistant technology, applied in the field of Bacillus megaterium and its application, can solve problems such as crop quality and soil impact, and achieve the effect of solving the serious damage of cyst nematode disease and solving the effect of low temperature in early spring

Inactive Publication Date: 2011-06-29
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method to improve the cold resistance of soybeans is to use some chemical substances or anti-cold agents, etc., which may have a certain impact on crop quality and soil

Method used

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  • Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof
  • Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Obtaining of Bacillus megaterium Sneb69

[0021] Take 1g of soybean field soil, add 100ml of sterile water, take 200μl, add 20ml of sterilized NA liquid medium cooled to below 35°C, cultivate at 25°C and wait for colonies to grow. Bacillus megaterium (Bacillus megaterium) colony is preserved separately, carries out multiplication and physiological and biochemical assay, and the culture trait conforms to the characteristic of Bacillus megaterium (Bacillus megaterium) and preserves for future use.

Embodiment 2

[0022] Embodiment 2: the cultivation of Bacillus megaterium (Bacillus megaterium) Sneb69

[0023] Firstly, the Bacillus megaterium (Bacillus megaterium) Sneb69 strain was fermented and cultured, and then the metabolites after fermentation and cultured were tested for soybean-inducing activity to determine their inducing effect on soybean.

[0024] The thalline of Bacillus megaterium (Bacillus megaterium) Sneb69 is inoculated on the test tube agar medium, and the medium formula is NA medium, namely composition: beef extract 3g, peptone 10g, yeast extract 1g, sucrose 10g, agar 20g, Add water to 1000mL. Incubate at 25°C for 10 days to obtain test tube seeds.

[0025] Test tube seed is inoculated in the liquid culture medium of 250mL triangular flask (every bottle 100mL) again, and medium formula is: by weight percentage, containing beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, The remainder was distilled water with a pH of 8. Cultivate at 25°C, shaker speed at...

Embodiment 3

[0026] Example 3: Fermentation of Bacillus megaterium Sneb69

[0027] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25° C., pH 8, and fermented for 48 hours.

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Abstract

The invention relates to a culture method of bacillus megaterium Sneb69 which can induce soybeans to withstand low temperature in early spring and soybean cyst nematode after processing soybean seeds and an application thereof, and belong to the technical field of microbial pesticides. A bacterial strain Sneb69 in the invention was collected in China General Microbiological Culture Collection Center on November 2, 2010 with the collection number of CGMCC No.4296. Through liquid fermentation, culture and preparation, the soybeans can withstand the low temperature in the early spring and the soybean cyst nematode after the soybean seeds are processed by metabolites. The soybeans after being processed by the metabolites of the bacteria can be sown in the early spring, and be grown in soil with serious soybean cyst nematode. By adopting the invention, difficult problems that the effective accumulated temperature for growth of the soybeans in high-altitude regions all the year around is less, and seasons which are suitable for the growth of the soybeans are short are solved, so that the culture method has a good application and development prospects.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Bacillus megaterium capable of inducing stress resistance in soybeans and its application. Background technique: [0002] Northeast my country is the main soybean producing area in China, and low temperature is the main factor reducing soybean production. Soybean is prone to chilling injury at all stages of the entire growth period, thereby reducing yield and quality, especially the early germination stage is sensitive to low temperature. When sowing in early spring, due to the low temperature, it is easy to prolong the emergence time and cause deformed seedlings, or the seeds will lose their germination ability due to mildew and deterioration, resulting in a reduction in soybean yield. Therefore, strengthening the relevant research on the low temperature tolerance of soybean emergence has positive significance for soybean production. The cold resista...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01C1/08C12R1/11
Inventor 段玉玺黄姗姗陈立杰王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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