Transgenic cotton event GH_CSM63718 and compositions and methods for detection and uses thereof

Abstract

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

Description

TITLE OF THE INVENTION TRANSGENIC COTTON EVENT GH_CSM63718 AND COMPOSITIONS AND METHODS FOR DETECTION AND USES THEREOF CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to U.S. Provisional Application Serial No. 63 / 656,474, filed June 5, 2024, the entire disclosure of which is incorporated herein by reference. INCORPORATION OF SEQUENCE LISTING

[0002] The sequence listing contained in the file named “MONS585WO_ST26.xml”, which is 341 kilobytes (measured in MS-Windows) and was created on May 8, 2025, is filed herewith by electronic submission, and is incorporated herein by reference in its entirety. FIELD

[0003] The present disclosure relates to compositions and methods for providing herbicide tolerance in transgenic cotton plants. Recombinant DNA molecules present in and / or isolated from cotton event Gh_CSM63718 are provided. Also provided are transgenic cotton plants, plant parts, seeds, cells, and agricultural products comprising the cotton event Gh_CSM63718, as well as methods of producing and using transgenic cotton plants, plant parts, seeds, cells, and agricultural products comprising cotton event Gh_CSM63718, methods of detecting cotton event Gh_CSM63718, and methods of controlling weeds. Transgenic cotton plants, plant parts, seeds and cells comprising cotton event Gh_CSM63718 exhibit tolerance to benzoic acid auxins such as dicamba; inhibitors of glutamine synthetase such as glufosinate; ß-triketone herbicides (inhibitors of 4-hydroxyphenylpyruvate dioxygenase, or HPPD) such as mesotrione, inhibitors of 5- enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate, and a variety of PPO herbicides. BACKGROUND

[0004] Increasing sustainable crop production is crucial to meet the need for food for the growing global population, feed for increased demand on animal-based diets in developing nations, and expanded use of crop products to produce biofuel, fiber, and other agricultural 1 US_ACTIVE\130153301\V-1product-based commodities, while using limited natural resources. In agricultural systems, the effective management of weedy species in agricultural fields is essential for maintaining favorable crop growing conditions and yield. Weeds compete with crops for space, nutrients, water, and sunlight and can lead to 25-80% yield losses. Selective herbicides had significantly contributed to weed management before the deployment of herbicide tolerant crops. Application of herbicides provides an important tool to reduce weed pressure, improve productivity and increase security for global crop production.

[0005] Cotton is a versatile commodity used in many products, particularly clothing. Each year about 27 million tons of cotton is produced globally. The introduction of genetically modified crops containing herbicide tolerance traits has successfully provided additional tools available to farmers to better control weeds. Transgenic herbicide tolerance enables the use of an herbicide in a crop growing environment without crop injury or with minimal crop injury (e.g., less than about 10% injury). Transgenic cotton traits have been used to impart tolerance to glyphosate and glufosinate and are used broadly in commercial cotton production for weed management. However, weeds have evolved resistance to herbicides and weed resistance continues to present a challenge in cotton production today. Therefore, there is a need for additional herbicide tolerance trait options to manage weeds effectively and to sustain crop productivity. One of the solutions is to employ herbicide(s) with new or different mode(s) of action, and / or employ multiple herbicides with different modes of action.

[0006] Combinations of herbicide tolerance traits are desirable to provide weed control options that increase grower flexibility and enable the use of multiple herbicide modes of action for controlling challenging weeds. Combining multiple desired traits in the genome can be achieved by several approaches: 1) by making crosses between two parents each having a desired trait at a randomly inserted site, and identifying progeny plants that have combination of the desired traits; 2) by retransforming a transgenic plant comprising one or more desired trait(s) with one or more genes for additional desired traits, either through random integration or through targeted integration of the one or more genes for additional desired traits; 3) by inserting multiple genes as a single DNA molecule into one location, or locus, in the genome, which provides a useful tool in weed control that is much simpler and less expensive to maintain during subsequent breeding into a diverse pool of elite germplasms; and 4) by targeting one or more desired traits to a specific 2 US_ACTIVE\130153301\V-1genomic location (site directed integration) carrying one or more desired traits, in a new transformation event, followed by crosses between the new event and another event carrying the one or more desired event at the specific genomic location, resulting in progeny plants that have combination of the desired traits at one location and segregate together.

[0007] The expression of transgenes in a transgenic plant, plant part, seed, cell or progeny, and thus their effectiveness, may be influenced by many factors, such as the regulatory elements used in the transgenes’ expression cassettes, the combination and / or interaction of these regulatory elements, the chromosomal location of the transgene insertion site, the chromatin structure of the genome at or near the transgene insertion site, and the presence or proximity of any endogenous cis and / or trans regulatory elements or genes close to the transgene insertion site. In addition, the performance of the traits in the transgenic plant is further complicated when the transgenic insert comprises multiple expression cassettes such as five independent expression cassettes in the present disclosure, each having a different transgene conferring a distinct trait. These differences or factors may result in variation in the level of transgene expression or in the spatial or temporal pattern of transgene expression among different transgenic insertion events of the same expression cassettes. Furthermore, different transgenic events can also vary in terms of the molecular quality of the events. For example, a transgenic event may contain two or more copies of the transgene insertion at one or more chromosomal locations, or a transgenic insertion may be truncated relative to the intended insertion or contain vector backbone sequences, or a transgene may be inserted into an endogenous gene or in a repeated region. In the case of site directed integration of desired traits, the machinery for site directed integration, such as gRNA or nuclease, which has to be excised from the commercial events, may not be completely removed. Such characteristics may result in undesirable outcomes, such as gene silencing, altered pattern and / or expression of the transgene, and / or altered pattern and / or expression of endogenous genes. There may also be undesirable phenotypic or agronomic differences among different events.

[0008] Even in the case of targeted sequence insertion, variability in the level of transgene expression between independent but genetically identical targeted sequence insertion (TSI) events was observed in a subset of transgenic events (Verkest et al., 2019). This expression variability and silencing occurred independently of the transgene sequence and could be attributed to DNA methylation that was further linked to different DNA methylation mechanisms. Transgene 3 US_ACTIVE\130153301\V-1integration into targeted loci through Cre-lox mediated recombination has also been reported to produce a large percentage of targeted integration events that showed a partial spatial pattern of transgene expression due to differential silencing (Day et al., 2000). The fact that a considerable variation in transgene expression was observed shows that even when integration events are targeted, selection remains necessary similarly to the practice for random integration events in order to identify targeted insertion events with stable and desirable gene of interest expression over generations.

[0009] A commercially useful transgenic event requires that the transgene(s) in the transgenic insert express in the manner necessary for that trait(s) to be successful, and involves rigorous testing, evaluation, and selection. Such tests include in vitro and / or in planta testing different regulatory elements (e.g., promoters, introns, leaders, and 3’ UTRs) and combinations of different regulatory elements for desirable spatial and temporal expression of the transgene(s), as well as examining whether to target the product of the transgene(s) (protein(s)) to subcellular compartments such as chloroplasts to select for the best expression cassette(s). For site directed integration of a transgene, once a targeted insertion strategy / method is chosen, the target sites are identified, screened and selected. The selected combinations of expression cassette(s), targeting sites and gRNA are then used for transformation to produce transgenic plants. It is also important to remove the selection marker gene efficiently from the events post-transformation to avoid regulatory concerns and to maintain sustainability.

[0010] For these reasons, the performance of different transformation events from the same transformation construct can vary widely, and the identification of transformation events conferring the most beneficial traits or characteristics without other potential off-types, concerns or marker gene is needed to select a superior event for commercial use. Therefore, a large number of individual transgenic events must be produced and analyzed to select an event having superior commercial properties, which can be a significant undertaking that involves analysis and selection among many different transformation events.

[0011] To establish a transgenic event for commercial use requires rigorous molecular characterization, greenhouse testing, and field trials over multiple years, in multiple locations and under a variety of conditions, allowing extensive agronomic, phenotypic, and molecular data to be obtained. The resulting data are then analyzed to select an event that is suitable for commercial 4 US_ACTIVE\130153301\V-1purposes. The commercial event, once identified as having the desired transgene expression, molecular characteristics, efficacy and field performance, can then be introgressed into other cotton genetic backgrounds using plant breeding methods. The resulting cotton varieties contain the new traits combined with other desirable qualities such as native traits, disease tolerance traits, insect control traits, high-yielding germplasms or traits, and / or one or more other transgenic herbicide tolerance traits. SUMMARY

[0012] A recombinant DNA molecule is provided. The recombinant DNA molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:10; SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9; and a complete complement of any of the foregoing. In some embodiments, the recombinant DNA molecule is derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product comprising cotton event Gh_CSM63718, a representative sample of seed comprising the event having been deposited as ATCC Accession No. PTA-127638. In some embodiments, the recombinant DNA molecule is comprised in a cotton plant, seed, plant part, plant cell, or progeny plant comprising cotton event Gh_CSM63718, or a commodity product produced therefrom, a representative sample of seed comprising the event having been deposited as ATCC Accession No. PTA-127638. The recombinant DNA molecule can be formed by the insertion of a heterologous nucleic acid molecule into the genomic DNA of a cotton plant or cotton cell. The recombinant DNA molecule can comprise an amplicon diagnostic for the presence of cotton event Gh_CSM63718.

[0013] DNA molecules that function as DNA probes are provided. An example of such a DNA molecule is a DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with cotton event Gh_CSM63718 DNA in a sample. Detecting hybridization of the DNA molecule under the 5 US_ACTIVE\130153301\V-1stringent hybridization conditions is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.

[0014] Also provided is a DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe specific for detecting in a sample at least one of: a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718; a 3’ junction sequence between the transgenic insert of cotton event Gh_CSM63718 and flanking cotton genomic DNA; SEQ ID NO:9; and a fragment of SEQ ID NO:9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO:9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718.

[0015] The DNA probe can comprise SEQ ID NO:21. Alternatively or in addition, the DNA probe can comprise a nucleotide sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; and a complement of any of the foregoing. The sample can be derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product.

[0016] A pair of DNA molecules is provided. The pair of DNA molecules comprises a first DNA molecule and a second DNA molecule. The first and the second DNA molecules are different from one another, and each comprise a fragment of SEQ ID NO:10 or a complement thereof and function as DNA primers when used together in an amplification reaction with DNA comprising cotton event Gh_CSM63718 to produce an amplicon diagnostic for cotton event Gh_CSM63718 in a sample. For example, the first and the second DNA molecules can comprise SEQ ID NO:19 and SEQ ID NO:20. The amplicon can comprise a nucleotide sequence selected from the group consisting of: SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; and a fragment of any of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, wherein the fragment is at least 10 nucleotides in length and comprises nucleotides 1,000–1,001 or 17,736-17,737 of SEQ ID NO:10. The sample can be derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product.

[0017] Methods for detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part, plant cell, progeny plant, or commodity product are provided. 6 US_ACTIVE\130153301\V-1In a first example of such a method, the method comprises: (a) contacting the sample with any of the DNA molecules that function as a DNA probes specific for cotton event Gh_CSM63718 described herein; (b) subjecting the sample and the DNA molecule that functions as a probe to stringent hybridization conditions; and (c) detecting the hybridization of the DNA molecule that functions as a probe to a DNA molecule in the sample. The hybridization of the DNA molecule that functions as a probe to the DNA molecule in the sample is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.

[0018] Another method of detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part or plant cell, progeny plant or commodity product is provided. The method comprises: (a) contacting the sample with any of the pairs of DNA molecules that can be used to produce an amplicon diagnostic for cotton event Gh_CSM63718 described herein; (b) performing an amplification reaction sufficient to produce a DNA amplicon; and (c) detecting the presence of the DNA amplicon. The DNA amplicon comprises at least one of: a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718, a 3’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718, SEQ ID NO: 9, and a fragment of SEQ ID NO: 9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO: 9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718. The presence of the DNA amplicon indicates the presence of cotton event Gh_CSM63718 in the sample. The DNA amplicon can be at least 10 nucleotides in length, at least 11 nucleotides in length, at least 12 nucleotides in length, at least 13 nucleotides in length, at least 14 nucleotides in length, at least 15 nucleotides in length, at least 16 nucleotides in length, at least 17 nucleotides in length, at least 18 nucleotides in length, at least 19 nucleotides in length, at least 20 nucleotides in length, at least 25 nucleotides in length, at least 30 nucleotides in length, at least 35 nucleotides in length, at least 40 nucleotides in length, at least 45 nucleotides in length, at least 50 nucleotides in length, at least 60 nucleotides in length, at least 70 nucleotides in length, at least 80 nucleotides in length, at least 90 nucleotides in length, or at least 100 nucleotides in length. The DNA amplicon can comprise a nucleotide sequence selected from the group consisting of SEQ ID NO:10; SEQ ID NO:9; SEQ ID NO:8; SEQ ID NO:7; SEQ ID NO:6; SEQ ID NO:5; SEQ ID NO:4; SEQ ID NO:3; SEQ ID NO:2; SEQ ID NO:1; and a fragment of any of SEQ ID NO:10, SEQ ID NO:8, SEQ ID NO:7, SEQ ID NO:6, 7 US_ACTIVE\130153301\V-1SEQ ID NO:5, SEQ ID NO:4, SEQ ID NO:3, SEQ ID NO:2, and SEQ ID NO:1 that is at least 10 nucleotides in length and comprises nucleotides 1,000–1,001 or 17,736-17,737 of SEQ ID NO:10.

[0019] A further method of detecting the presence of cotton event Gh_CSM63718 in a sample of DNA derived from a cotton seed, plant, plant part, plant cell, progeny plant or commodity product is provided. The method comprises: (a) contacting the sample with any of the DNA molecules that function as a probe specific for cotton event Gh_CSM63718 described herein; and (b) performing a sequencing reaction to produce a target sequence. The target sequence comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; a complete complement of any thereof; and a fragment of any of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10 that is at least 10 nucleotides long and comprises nucleotides 1,000–1,001 or 17,736-17,737 of SEQ ID NO:10.

[0020] Another method of detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part, cell, progeny plant or commodity product is provided. The method comprises: (a) contacting the sample with an antibody specific for the PPO protein encoded by cotton event Gh_CSM63718, an antibody specific for the TDO protein encoded by cotton event Gh_CSM63718, or a combination thereof; and (b)detecting binding of the antibody or antibodies to the protein or proteins in the sample. The binding of the antibody or antibodies indicates the presence of cotton event Gh_CSM63718 in the sample. The method can further comprise: (a) contacting the sample with an antibody specific for the DMO protein encoded by cotton event Gh_CSM63718, an antibody specific for the EPSPS protein encoded by cotton event Gh_CSM63718, an antibody specific for the PAT protein encoded by cotton event Gh_CSM63718, or a combination of any thereof; and (b) detecting binding of the antibody or antibodies to the protein or proteins in the sample. The binding of the antibody or antibodies indicates the presence of cotton event Gh_CSM63718 in the sample.

[0021] DNA detection kits for detecting the presence of cotton event Gh_CSM63718 in a sample are provided. One example of such a DNA detection kit is a kit comprising: (a) any of the pairs of DNA molecules that can be used to produce an amplicon diagnostic for cotton event 8 US_ACTIVE\130153301\V-1Gh_CSM63718 described herein; and / or (b) any of the DNA molecules that function as a probe specific for cotton event Gh_CSM63718 described herein.

[0022] Also provided are protein detection kits for detecting the presence of cotton event Gh_CSM63718 in a sample. The kit comprises an antibody specific for the PPO protein encoded by cotton event Gh_CSM63718, an antibody specific for the TDO protein encoded by cotton event Gh_CSM63718, or a combination thereof. Detecting binding of the antibody or antibodies to the protein(s) encoded by cotton event Gh_CSM63718 in a sample is diagnostic for the presence of cotton event Gh_CSM63718 in the sample. The protein detection kit can further comprise an antibody specific for the DMO protein encoded by cotton event Gh_CSM63718, an antibody specific for the EPSPS protein encoded by cotton event Gh_CSM63718, an antibody specific for the PAT protein encoded by cotton event Gh_CSM63718, or a combination of any thereof.

[0023] Methods of determining the zygosity of a cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718 are provided. One example of such a method comprises: (a) contacting a sample comprising DNA derived from the cotton plant, plant part, plant seed, or plant cell with a first primer set capable of producing a first amplicon diagnostic for the presence of cotton event Gh_CSM63718, and a second primer set capable of producing a second amplicon diagnostic for wildtype cotton genomic DNA not comprising cotton event Gh_CSM63718; (b) performing a nucleic acid amplification reaction; and (c) detecting the first amplicon and the second amplicon. The presence of both amplicons indicates that the plant, plant part, seed or cell is heterozygous for cotton event Gh_CSM63718. The presence of only the first amplicon indicates that the plant, plant part, seed, or cell is homozygous for cotton event Gh_CSM63718. For example, the first primer set can comprise SEQ ID NO:19 and SEQ ID NO:20, and the second primer set comprises SEQ ID NO:19 and SEQ ID NO:22.

[0024] Another method of determining the zygosity of a cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718 is provided. The method comprises: (a) contacting a sample comprising DNA derived from the cotton plant, plant part, plant seed, or plant cell with a probe set comprising at least a first probe that specifically hybridizes to cotton event Gh_CSM63718, and at least a second probe that specifically hybridizes to cotton genomic DNA that was disrupted by insertion of the heterologous DNA of cotton event Gh_CSM63718 but does not hybridize to cotton event Gh_CSM63718; and (b) hybridizing the probe set with the sample 9 US_ACTIVE\130153301\V-1under stringent hybridization conditions. Detecting hybridization of only the first probe under the hybridization conditions is diagnostic for a cotton plant, plant part, seed or plant cell homozygous for cotton event Gh_CSM63718. Detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a cotton plant, plant part, seed, or plant cell heterozygous for cotton event Gh_CSM63718. For example, the probe set can comprise SEQ ID NO:21 and SEQ ID NO:23.

[0025] DNA constructs are provided. One example of a DNA construct provided herein is a DNA construct comprising a first expression cassette, a second expression cassette, a third expression cassette, a fourth expression cassette, and a fifth expression cassette. The first expression cassette comprises in operable linkage: (i) a ribulose bisphosphate carboxylase / oxygenase (RuBisCO) activase gene promoter, and a leader sequence from Arabidopsis thaliana, (ii) a codon-optimized phosphinothricin N-acetyltransferase (PAT) coding sequence from Streptomyces viridochromogenes, and (iii) a 3’ UTR of a small heat shock protein (Hsp20) from Medicago truncatula. The second expression cassette comprises in operable linkage: (i) an enhancer from the strawberry vein banding virus (SVBV) fused to the promoter and 5’ UTR from a CAB1 (Chlorophyll A / B Binding Protein) gene from Cucumis melo, (ii) a codon-optimized triketone dioxygenase (TDO) coding sequence from Oryza sativa, and (iii) a 3’ UTR of a TMA7 (translation machinery associated 7) protein from Medicago truncatula. The third expression cassette comprises in operable linkage: (i) a polyubiquitin gene (UBQ10) promoter, a leader and an intron sequence from Arabidopsis thaliana, (ii) an N-terminal chloroplast transit peptide coding sequence of APG6 (Albino and Pale Green 6) from Arabidopsis thaliana fused to a codon- optimized dicamba monooxygenase (DMO) coding sequence from Stenotrophomonas maltophilia; and (iii) a 3’ UTR of an aluminum-induced Sali3-2 protein from Medicago truncatula. The fourth expression cassette comprises in operable linkage: (i) an enhancer of the 35S gene from Figwort Mosaic Virus (FMV), (ii) a promoter, a leader sequence, and an intron sequence of the elongation factor 1A gene (ELF1a) from Arabidopsis thaliana, (iii) an N-terminal chloroplast transit peptide of granule bound starch synthase I from Triticum aestivum fused to a codon optimized 5-enolpyruvylshikimate-3-phosphate synthase gene (EPSPS) from Agrobacterium sp strain CP4, and (iv) a 3’ UTR of a ribulose 1,5-bisphosphate carboxylase small subunit E9 (rbcS- E9) gene from Pisum sativum. The fifth expression cassette comprises in operable linkage: (i) an 10 US_ACTIVE\130153301\V-1enhancer derived from multiple enhancer sequences from Arabidopsis thaliana, (ii) a promoter sequence designed from multiple promoter sequences from Arabidopsis thaliana, (iii) an intron and 5’ UTR for a cytochrome C oxidase subunit VIa gene from Arabidopsis thaliana fused a 5’ UTR designed from multiple 5’ UTR sequences from Arabidopsis thaliana, (iv) an N-terminal chloroplast transit peptide coding sequence of APG6 (Albino and Pale Green 6) from Arabidopsis thaliana, with monocot codon usage, fused to the coding region of a protoporphyrinogen oxidase (PPO) gene from Enterobacter cloacae with codons optimized for cotton, and (v) a 3’ UTR from the fiber FbLate-2 gene from Gossypium barbadense. For example, the DNA construct can comprise SEQ ID NO:9. The DNA construct can further comprise at the 5’ and / or 3’ end of said construct: (a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14; and / or (b) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:12 or SEQ ID NO:15.

[0026] Another DNA construct is provided. The DNA construct comprises a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the full length of SEQ ID NO: 9. The DNA construct comprises at the 5’ and / or 3’ end of said construct (i) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of 11 US_ACTIVE\130153301\V-1SEQ ID NO:11 or SEQ ID NO:14; and / or (ii) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:15.

[0027] Any of the DNA constructs can comprise at the 5’ end of said construct one or more nucleotide sequences selected from SEQ ID NOs:58–127. Any of the DNA constructs can comprise at the 3’ end of said construct one or more nucleotide sequences selected from SEQ ID NOs:128–197.

[0028] Methods for controlling or preventing weed growth in an area are provided. One example of such a method comprises planting cotton comprising event Gh_CSM63718 in the area and applying an effective amount of at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and any combination thereof, to control weeds in the area without injury to the cotton or with less than about 10% injury to the cotton. Applying the effective amount of at least one herbicide can comprise applying at least two or more herbicides selected from the group consisting of glufosinate, a β -triketone HPPD inhibitor, dicamba, glypohsate, a PPO herbicide, and any combination thereof over a growing season.

[0029] Methods for controlling volunteer cotton comprising cotton event Gh_CSM63718 in an area are provided. One example of such a method comprises applying an herbicidally effective amount of at least one herbicide other than glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, or a PPO herbicide. The herbicide application prevents growth of cotton comprising cotton event Gh_CSM63718. The herbicide other than glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, or a PPO herbicide can be selected from the group consisting of atrazine, topramezone, clopyralid, pyrithiobac, fluometuron, (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU), 2,4-D, thidiazuron, dichlorprop-p 2-ethylhexyl ester, dichlorprop-p, trifloxysulfuron, paraquat, diquat, and combinations of any thereof.

[0030] Methods of obtaining a seed of a cotton plant or a cotton plant that is tolerant to glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, or any 12 US_ACTIVE\130153301\V-1combination thereof are provided. One example of such a method comprises: (a) obtaining a population of progeny seed or plants grown therefrom, at least one of which comprises cotton event Gh_CSM63718; and (b) identifying at least a first progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718. Identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 can comprise: (a) growing the progeny seed or plant to produce progeny plants; (b) treating the progeny plants with an effective amount of at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof; and (c) selecting a progeny plant that is tolerant to the at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof. Alternatively or in addition, identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 can comprise detecting the presence of cotton event Gh_CSM63718 in a sample derived from the progeny seed or plant grown therefrom. Alternatively or in addition, identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 can comprise detecting the presence of at least one protein encoded by cotton event Gh_CSM63718 in a sample derived from the progeny seed or plant grown therefrom.

[0031] Methods of improving tolerance to at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof in a cotton plant are provided. One example of such a method comprises: (a) inserting any of the DNA constructs described herein into the genome of a cotton cell; (b) generating a cotton plant from the cotton cell; and c) selecting a cotton plant comprising the DNA construct. The selecting can comprise treating the cotton cell or plant with an effective amount of at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof.

[0032] Cotton plants, plant seeds, plant parts, and plant cells comprising a recombinant DNA molecule are provided. The recombinant DNA molecule comprises a sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at 13 US_ACTIVE\130153301\V-1least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9; and a complete complement of any of the foregoing. The cotton plant, plant seed, plant part, or plant cell expresses at least one herbicide tolerance gene selected from the group consisting of phosphinothricin N-acetyltransferase (PAT), triketone dioxygenase (TDO), dicamba monooxygenase (DMO), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), protoporphyrinogen oxidase (PPO), and any combination thereof. The cotton plant, plant seed, plant part, or plant cell is tolerant to at least one herbicide selected from the group consisting of glufosinate, β-triketone HPPD inhibitors, dicamba, glyphosate, PPO herbicides, and combinations of any thereof.

[0033] Further cotton plants, plant seeds, plant parts, and plant cells are provided. The cotton plants, plant seeds, plant parts, and plant cells are tolerant to at least one herbicide selected from the group consisting of glufosinate, β-triketone HPPD inhibitors, dicamba, glyphosate, PPO herbicides, and combinations of any thereof. The cotton plants, plant seeds, plant parts, or plant cells comprise any of the DNA constructs described herein.

[0034] Any of the cotton plants, plant seeds, plant parts, or plant cells can comprise cotton event Gh_CSM63718, a representative sample of seed comprising the event having been deposited under ATCC Accession No. PTA-127638.

[0035] Any of the cotton plants, plant seeds, plant parts, or plant cells can be further defined as a progeny plant of any generation of a cotton plant comprising cotton event Gh_CSM63718, or a cotton plant part, plant seed, or plant cell derived therefrom.

[0036] A further cotton plant, plant part, plant seed, or plant cell is provided. The cotton plant, plant part, plant seed, or plant cell comprises cotton event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638.

[0037] The cotton plant part can comprise a microspore, pollen, an anther, an ovule, an ovary, a boll, a flower, an embryo, a stem, a bud, a node, a leaf, a root, or a callus. 14 US_ACTIVE\130153301\V-1

[0038] Any of the cotton plants, plant seeds, plant parts, or plant cells can be obtained by any of the methods of obtaining a seed of a cotton plant or a cotton plant, or any of the methods of improving tolerance to at least one herbicide described herein.

[0039] A further cotton plant, plant part, plant seed, or plant cell is provided. The cotton plant, plant cell, plant part, or plant seed comprises a recombinant DNA construct integrated in chromosome 21. The recombinant DNA construct confers tolerance to at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof. The recombinant DNA construct is integrated in a position of said chromosome flanked by at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14; and / or (ii) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:15. The at least 50 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14 can comprise one or more nucleotide sequences selected from SEQ ID NOs:58-127. The at least 50 contiguous nucleotides of SEQ ID NO:12 or SEQ ID NO:15 can comprise one or more nucleotide sequences selected from SEQ ID NOs:128- 197.

[0040] With respect to any of the cotton plants, plant cells, plant parts, and plant seeds, and any of the methods for controlling or preventing weed growth in an area, any of the methods of obtaining a seed of a soybean plant or a soybean plant, and any of the methods of improving tolerance to at least one herbicide, the β-triketone HPPD inhibitor can be selected from the group consisting of mesotrione, benzobicyclon (BBC), tembotrione, sulcotrione, tefuryltrione, and combinations of any thereof. The PPO herbicide can be selected from the group consisting of diphenylethers, N-phenylphthalimides, oxadiazoles, oxazolidinediones, phenylpyrazoles, 15 US_ACTIVE\130153301\V-1pyrimidinediones, thiadiazoles, triazolinones, benzoxazinone derivatives, other PPO herbicides, and combinations of any thereof. The diphenylether can be selected from the group consisting of acifluorfen, bifenox, ethoxyfen, fluorodifen, fluoronitrofen, furyloxyfen, halosafen, chlomethoxyfen, chlornitrofen, ethoxyfen-ethyl, fluoroglycofen, lactofen, nitrofen, oxyfluorfen, fomesafen, a salt of any thereof, and an ester of any thereof. The N-phenylphthalimide can be selected from the group consisting of cinidon-ethyl, flumiclorac, flumiclorac-pentyl, and flumioxazin. The oxadiazole can be selected from the group consisting of oxadiargyl and oxadiazon. The oxazolidinedione can be pentoxazone. The phenylpyrazole can be selected from the group consisting of fluazolate, pyraflufen, and pyraflufen-ethyl. The pyrimidinedione can be selected from the group consisting of benzfendizone, butafenacil, epyrifencacil (S-3100), flupropacil, flufenoximacil, saflufenacil, and tiafenacil. The thiadiazole can be selected from the group consisting of fluthiacet-methyl and thidiazimin. The triazolinone can be selected from the group consisting of azafenidin, bencarbazone, carfentrazone, its salts and esters, and sulfentrazone. The benzoxazinone derivative can be 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,4-dihydro-3-oxo- 4-prop-2-ynyl-2H-1,4-benzoxazin-6-yl)-1,3,5-triazinane-2,4-dione (trifludimoxazin)). The other PPO herbicide can be selected from the group consisting of chlorphthalim, flufenpyr, flufenpyr- ethyl, flumipropyn, pyraclonil, profluazol, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2- yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2- methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}pyridin-2-yl)oxy]acetate; cyclopropylmethyl (2-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}phenoxy)acetate; methyl 2- {[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} propanoate (flufenoximacil), methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} propanoate, methyl 2- 16 US_ACTIVE\130153301\V-1{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}-2-methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} butanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate methyl (2R)-2- 17 US_ACTIVE\130153301\V-1({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)- [2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, 2-{[(E)-{5- [3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo- 4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole- 5-carboxylate, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5- dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- 18 US_ACTIVE\130153301\V-1(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5- [3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-propyl-4,5- dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5- carboxylate, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5- dihydro-1,2-oxazol-3-yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5- dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro- 1,2-oxazole-5-carboxylate, 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5- methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo- 4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5- dihydro-1,2-oxazol-3-yl)phenyl]-1,5-dimethyl-6-sulfanylidene-1,3,5-triazinane-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5- dihydro-1,2-oxazole-5-carboxylic acid, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, methyl 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo- 4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, 2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3- 19 US_ACTIVE\130153301\V-1methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, {[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid, 2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, ethyl [(3-{2-chloro-5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2- yl)oxy]acetate, [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin- 1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetic acid, ethyl (2-{2-chloro-5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}phenoxy)acetate, (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}phenoxy)acetic acid, ethyl (2-{2-chloro-4-fluoro- 5-[4-(1-fluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, 2-methoxyethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl- 2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, tetrahydrofuran-2-ylmethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2- chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}pyridin-2-yl)oxy]acetate, methyl (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl- 2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}phenoxy)(methoxy)acetate, methyl (2-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}phenoxy)(methoxy)acetate, [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6- dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetic acid, ethyl [(3- {2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)- 3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, tetrahydrofuran-2-ylmethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, ethyl 2-[[3-[5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-pyrimidin-1-yl]-4-fluoro-2-nitro-phenoxy]- 2- pyridyl]oxy]acetate, 1-ethoxy-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2- 20 US_ACTIVE\130153301\V-1chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, ,2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, and ethyl 1-({1- [2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate. 21 US_ACTIVE\130153301\V-1

[0041] In any of the methods involving the use of an effective amount of glufosinate, the effective amount of glufosinate can be about 0.4 lb / acre to about 1.6 lb / acre over a growing season. In any of the methods involving the use of an effective amount of a , a β-triketone HPPD inhibitor, the β-triketone HPPD inhibitor can comprise mesotrione and the effective amount of mesotrione can be about 0.09 lb / acre to about 0.36 lb / acre. In any of the methods involving the use of an effective amount of dicamba, the effective amount of dicamba can be about 0.5 lb / acre to about 2 lb / acre over a growing season. In any of the methods involving the use of an effective amount of glyphosate, the effective amount of glyphosate can be about 0.5 lb / acre to about 2.5 lb / acre over a growing season. In any of the methods involving the use of an effective amount of a PPO herbicide, the effective amount of the PPO herbicide can be about 0.0009 lb / acre to about 1.5 lb / acre over a growing season.

[0042] A method of producing a progeny cotton plant comprising cotton event Gh_CSM63718 is provided. The method comprises: (a) sexually crossing a first cotton plant that comprises cotton event Gh_CSM63718 with itself or a second cotton plant; (b) collecting one or more seeds produced from the cross; (c) growing one or more seeds to produce one or more progeny plants; and (d)selecting at least a first progeny plant or seed comprising cotton event Gh_CSM63718. Inbred or hybrid cotton plants and seeds comprising cotton event Gh_CSM63718 that are produced by this method are also provided herein.

[0043] A nonliving or nonregenerable cotton plant material is provided. The nonliving or nonregenerable cotton plant material comprises any of the recombinant DNA molecules provided herein or any of the DNA constructs provided herein.

[0044] Another nonliving or nonregenerable cotton plant material is provided. The nonliving or nonregenerable cotton plant material comprises cotton event Gh_CSM63718, a representative sample of seed comprising the cotton event cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638.

[0045] A commodity product is provided. The commodity product comprises any of the recombinant DNA molecules provided herein or any of the DNA constructs provided herein. The commodity product can be produced from a transgenic cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718. The commodity product can comprise whole or processed seeds; viable or nonviable seeds; viable plant parts (such as roots, nodes, bolls, buds or 22 US_ACTIVE\130153301\V-1leaves); viable plant cells; processed plant parts; processed plant tissues; dehydrated plant tissues; dehydrated plant parts; frozen plant tissues; frozen plant parts; food for human consumption such as cottonseed oil; plant parts processed for animal feed such as cottonseed meal and cottonseed hulls; cotton fiber; or cotton linters.

[0046] A method of producing a commodity product is provided. The method comprises: (a) obtaining a transgenic cotton plant, plant part, or plant seed comprising cotton event Gh_CSM63718; and (b) producing a commodity product from the transgenic cotton plant, plant part, or plant seed.

[0047] A method of controlling, preventing, or reducing the development of herbicide-tolerant weeds is provided. The method comprises cultivating in a crop growing environment a cotton plant comprising transgenes that provide tolerance to glufosinate, β-triketone HPPD inhibitor herbicides, dicamba, glyphosate, and PPO herbicides.

[0048] Another method for controlling, preventing, or reducing the development of herbicide- tolerant weeds is provided. The method comprises: (a) cultivating in a crop growing environment a cotton plant comprising any of the DNA constructs described herein or event Gh_CSM63718; and (b) applying to the crop growing environment at least one herbicide selected from the group consisting of glufosinate, a β-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and any combination thereof, wherein the cotton plant is tolerant to the at least one herbicide.

[0049] In any of the methods for controlling, preventing, or reducing the development of herbicide-tolerant weeds, the transgenes that provide tolerance to the herbicides can be present at a single genomic location in the cotton plant.

[0050] A method of reducing loci for cotton breeding is provided. The method comprises inserting a construct comprising transgenes that provide tolerance to glufosinate, β-triketone HPPD inhibitor herbicides, dicamba, glyphosate and PPO herbicides as a single locus at a genomic location in a cotton plant.

[0051] Any of the cotton plants, plant cells, plant parts, or plant seeds, any of the nonliving or nonregenerable cotton plant materials, any of the commodity products, and in any of the methods described herein, the cotton plant, plant cell, plant part, plant seed, nonliving or nonregenerable cotton plant material, or commodity product can further comprises one or more transgenes for providing resistance to insect infestations. The transgenes for providing resistance to insect 23 US_ACTIVE\130153301\V-1infestations can be selected from the group consisting of Cry1B.3, Cry1Da_7, Vip3Cb1.1, Cry2Ab2, and combinations of any thereof. The Cry1B.3 transgene can comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:225. The Cry1Da_7 transgene can comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:227. The Vip3Cb1.1 transgene can comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:229. The Cry2Ab2 transgene can comprise a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:231. The cotton plant, plant cell, plant part, plant seed, nonliving or nonregenerable plant material, or commodity product can further comprise cotton event Gh_BCS246002 and / or cotton event MON15947. The transgenes for providing resistance to insect infestations can provide resistance to infestations by Lepidopteran pests selected from the group consisting of Cotton Bollworm (Helicoverpa zea), Tobacco Budworm (Heliothis virescens), Fall Armyworm (Spodoptera frugiperda), Old World Bollworm (Helicoverpa armigera), and combinations of any thereof. The transgenes in Gh_BCS246002 for providing resistance to insect infestations are present at a single genomic location in the cotton plant. The cotton plant, plant cell, plant part, seed can further comprises a recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:212; SEQ ID NO:213; SEQ ID NO:214; SEQ ID NO:215; SEQ ID NO:216; SEQ ID NO:217; SEQ ID NO:218; SEQ ID NO:219; SEQ ID NO:220; SEQ ID NO:221; a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:212 or the full length of SEQ ID NO: 213; and a complete complement of any of the foregoing.

[0052] Further cotton plants, plant cells, plant parts, seeds, nonliving or nonregenerable cotton plant materials, and cotton commodity products are provided. The cotton plants, plant cells, plant parts, seeds, nonliving or nonregenerable cotton plant materials, and cotton commodity products comprise a foreign DNA at an insertion site in the cotton genome. The insertion site has a nucleic acid sequence at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 24 US_ACTIVE\130153301\V-1100% identical to the sequence of SEQ ID NO:13. The foreign DNA can be flanked by 5’ and 3’ flanking regions, wherein said 5’ flanking region is upstream of and contiguous with said foreign DNA and comprises SEQ ID NO:11, and wherein said 3’ flanking region is downstream of and contiguous with said foreign DNA and comprises SEQ ID NO:12. The foreign DNA can comprise at least one herbicide tolerance gene selected from the group consisting of a phosphinothricin N- acetyltransferase (PAT) gene, a triketone dioxygenase (TDO) gene, a dicamba monooxygenase (DMO) gene, a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, a protoporphyrinogen oxidase (PPO) gene, and any combination of any thereof. For example, the foreign DNA can comprise a phosphinothricin N-acetyltransferase (PAT) gene, a triketone dioxygenase (TDO) gene, a dicamba monooxygenase (DMO) gene, a 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) gene, and a protoporphyrinogen oxidase (PPO) gene. The foreign DNA can comprise SEQ ID NO: 9. Alternatively, the foreign DNA can comprise SEQ SEQ ID NO:9 with one or more modifications. The one or more modifications can comprise one or more insertions, one or more deletions, one or more substitutions, or a combination of any thereof, within SEQ ID NO:9. For example, the one or more modifications can comprise a deletion of all or a portion of the phosphinothricin N-acetyltransferase (PAT) gene, a deletion of all or a portion of the triketone dioxygenase (TDO) gene, a deletion of all or a portion of the dicamba monooxygenase (DMO) gene, a deletion of all or a portion of the 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) gene, a deletion of all or a portion of the protoporphyrinogen oxidase (PPO) gene, or a combination of any thereof. Alternatively or in addition, the one or more modifications can comprise an insertion of an additional expression cassette.

[0053] A method of producing a cotton plant or seed is provided. The method comprises inserting foreign DNA at an insertion site in the genome of the cotton plant or seed. The insertion site has a nucleic acid sequence at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the sequence of SEQ ID NO:13. The cotton plant produced by the method can have improved trait efficacy, increased expression of one or more transgenes, improved stability of one or more transgenes, improved agronomic characteristics, or any 25 US_ACTIVE\130153301\V-1combination thereof, as compared to a plant that has the same foreign DNA inserted at a different insertion site in the genome. Cotton plants and seeds produced by the method are also provided.

[0054] Another method of producing a cotton plant or seed is provided. The method comprises obtaining a cotton plant or seed comprising cotton event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638, and inserting foreign DNA into the sequence of SEQ ID NO:10 of the plant or seed. Cotton plants and seeds produced by the method are also provided.

[0055] Yet another method of producing a cotton plant or seed is provided. The method method comprises obtaining a cotton plant or seed comprising event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638, and deleting all or a portion of the sequence of SEQ ID NO:10 in the plant or seed. Cotton plants and seeds produced by the method are also provided.

[0056] A method of detecting the presence a nucleic acid molecule encoding PPO_H_N90 in a sample derived from a cotton seed, plant, plant part or plant cell, progeny plant, or commodity product is provided. The method comprises: (a) contacting the sample with a pair of DNA molecules; (b) performing an amplification reaction sufficient to produce a DNA amplicon comprising the nucleic acid encoding the PPO_H_N90 or a portion of the nucleic acid encoding the PPO_H_N90 of sufficient length to identify the presence of the nucleic acid encoding the PPO_H_N90; and (c) detecting the presence of the DNA amplicon. The presence of the DNA amplicon indicates the presence of PPO_H_N90 in the sample. The pair of DNA molecules can comprise SEQ ID NO:234 and SEQ ID NO:235.

[0057] Another method of detecting the presence of a nucleic acid molecule encoding PPO_H_N90 in a sample derived from a cotton seed, plant, plant part, plant cell, progeny plant, or commodity product is provided. The method comprises: (a) contacting the sample with a DNA molecule that functions as a DNA probe specific for the nucleic acid molecule encoding PPO_H_N90; (b) subjecting the sample and the DNA molecule that functions as a probe to stringent hybridization conditions; and (c) detecting the hybridization of the DNA molecule that functions as a probe to a DNA molecule in the sample. The hybridization of the DNA molecule that functions as a probe to the DNA molecule in the sample is diagnostic for the presence of 26 US_ACTIVE\130153301\V-1PPO_H_N90 in the sample. The DNA molecule that functions as a probe can comprise SEQ ID NO:236. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 illustrates the sequence of the cotton event Gh_CSM63718. Horizontal lines correspond to the positions of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:12 relative to SEQ ID NO:10. The horizontal arrows (SEQ ID NO:19 and SEQ ID NO:20) represent the approximate positions of an illustrative primer pair that can be used to detect cotton event Gh_CSM63718. The horizontal line labeled SEQ ID NO:21 represents the approximate position of an illustrative DNA probe that can be used to detect cotton event Gh_CSM63718. “RB” refers to the Agrobacterium T-DNA right border; “LB” refers to the Agrobacterium T-DNA left border. “PAT” represents a phosphinothricin N-acetyltransferase expression cassette; “TDO” represents a triketone dioxygenase expression cassette; “DMO” represents a dicamba monooxygenase expression cassette; “CP4” represents a 5-enolpyruvylshikimate-3-phosphate synthase expression cassette; “PPO” represents a protoporphyrinogen oxidase expression cassette; “Lox” represents a lox recombination site; and “ISR” represents a non-coding intergenic sequence designed to minimize potential effects of neighboring genes on each others’ expression. The horizontal line labeled SEQ ID NO:13 represents the relative location or position of the wildtype cotton genome where the transgenes (SEQ ID NO:9) were inserted. The dashed line represents a 103_nucleotide deletion in the cotton genome at the site of insertion.

[0059] Figure 2 is a diagrammatic representation of the T-DNA cassettes in the Agrobacterium Ti plasmid used to transform and generate cotton event Gh_CSM63718 before and after T-DNA integration, and after Cre-mediated excision of the marker and Cre cassettes. “RB” refers to the Agrobacterium T-DNA right border; “LB” refers to the Agrobacterium T-DNA left border. “aadA”, “Cre”, and “ISR1” and “ISR2” represent the aadA selectable marker cassette, the Cre recombinase cassette, and non-coding intergenic sequences, respectively. “PAT” represents a phosphinothricin N-acetyltransferase expression cassette; “TDO” represents a triketone dioxygenase expression cassette; “DMO” represents a dicamba monooxygenase expression 27 US_ACTIVE\130153301\V-1cassette; “CP4” represents 5-enolpyruvylshikimate-3-phosphate synthase expression cassette; “PPO” represents a protoporphyrinogen oxidase expression cassette. “Lox” represents a lox recombination site. “5’ Flank” and “3’ Flank” represent the 5’ and 3’ flanking cotton genomic sequences at the site of T-DNA integration, respectively. A: “T-DNA Before Integration” represents T-DNA comprising the aadA and Cre cassettes, the ISR1, the PAT, TDO, DMO, and CP4 cassettes, the ISR2, and the PPO cassette before transformation; B: “Inserted T-DNA After Integration” represents T-DNA comprising the aadA and Cre cassettes, the ISR1, the PAT, TDO, DMO, and CP4 cassettes, the ISR2, and the PPO cassette integrated into the cotton genome after transformation; C: “Inserted T-DNA After Cre-Excision” represents the integrated T-DNA cassettes after the aadA, Cre, and ISR1 were excised, leaving behind one of the two Lox sites, the PAT, TDO, DMO, CP4 and PPO cassettes, ISR2, and the left and right borders.

[0060] Figure 3 illustrates the approximate timelines for the research, testing, and development, leading to selection and identification of the commercial cotton event Gh_CSM63718. “POC” stands for Proof of Concept; “TFN” strands for Transformation; “GH” stands for Greenhouse; “NA” stands for North America.

[0061] Figure 4 is a diagrammatic representation of the breeding process to produce marker- free cotton events. “R0 Transformants” are the initial transgenic events generated by transformation with the transformation vector, which were hemizygous for the T-DNA allele comprising the aadA, and the Cre cassette, and an intergenic sequence region (ISR1) flanked by two lox recombination sites, followed by the PAT, TDO, DMO, CP4, ISR2 and PPO cassettes. The R0 transformants were self-pollinated to produce the R1 generation seeds, wherein many of the progeny lost the aadA and the Cre cassettes, and ISR1 flanked by the two Lox sites due to Cre- recombinase excision. Hemizygous, T-DNA positive, aadA, Cre, and ISR1 negative (also referred to as “marker-free”) plants were selected and self-pollinated, resulting in an R2 generation. R2 plants homozygous for the inserted T-DNA allele without the aadA, CRE, and ISR1 were selected and self-pollinated to produce a pure line of R3 Gold Standard Seed (GSS). The process continued to produce subsequent generations for generation stability studies and for segregation studies and for trait integration into other germplasms. The R2, R3, and R4 generation plants which were marker-free and homozygous for the inserted T-DNA were used for efficacy and agronomic field trials. Subsequent “R” generations (R1, R2, R3 and so on) represent successive generations 28 US_ACTIVE\130153301\V-1produced through self-pollination of plants derived from the initial R0 transformant that resulted in the cotton event Gh_CSM63718. BRIEF DESCRIPTION OF THE SEQUENCES

[0062] SEQ ID NO:1 is a 30-nucleotide sequence representing the 5’ junction region of the cotton genomic DNA and the integrated transgene insert. SEQ ID NO:1 corresponds to nucleotide positions 986-1,015 of SEQ ID NO:10.

[0063] SEQ ID NO:2 is a 30-nucleotide sequence representing the 3’ junction region of the integrated transgene insert and the cotton genomic DNA. SEQ ID NO:2 corresponds to nucleotide positions 17,722-17,751 of SEQ ID NO:10.

[0064] SEQ ID NO:3 is a 60-nucleotide sequence representing the 5’ junction region of the cotton genomic DNA and the integrated transgene insert. SEQ ID NO:3 corresponds to nucleotide positions 971-1,030 of SEQ ID NO:10.

[0065] SEQ ID NO:4 is a 60-nucleotide sequence representing the 3’ junction region of the integrated transgene insert and the cotton genomic DNA. SEQ ID NO:4 corresponds to nucleotide positions 17,707-17,766 of SEQ ID NO:10.

[0066] SEQ ID NO:5 is a 100-nucleotide sequence representing the 5’ junction region of the cotton genomic DNA and the integrated transgene insert. SEQ ID NO:5 corresponds to nucleotide positions 951-1,050 of SEQ ID NO:10.

[0067] SEQ ID NO:6 is a 100-nucleotide sequence representing the 3’ junction region of the integrated transgene insert and the cotton genomic DNA. SEQ ID NO:6 corresponds to nucleotide positions 17,687-17,786 of SEQ ID NO:10.

[0068] SEQ ID NO:7 is a 1,050-nucleotide sequence representing the 5’ genomic flank region of the cotton genomic DNA and 50 bp of the integrated transgene insert. SEQ ID NO:7 corresponds to nucleotide positions 1-1,050 of SEQ ID NO:10.

[0069] SEQ ID NO:8 is a 1,050-nucleotide sequence representing 50 bp of the 3’ junction region of the integrated transgene insert and the 3’ genomic flank region of the cotton genomic DNA. SEQ ID NO:8 corresponds to nucleotide positions 17,687-18,736 of SEQ ID NO:10. 29 US_ACTIVE\130153301\V-1

[0070] SEQ ID NO:9 is a 16,736-nucleotide sequence corresponding to the transgene insert of cotton event Gh_CSM63718. SEQ ID NO:9 corresponds to nucleotide positions 1,001-17,736 of SEQ ID NO:10.

[0071] SEQ ID NO:10 is a 18,736-nucleotide sequence corresponding to the contig nucleotide sequence of the 5’ cotton genomic DNA sequence (SEQ ID NO:11), three random nucleotides inserted during T-DNA integration, the transgene insert in event Gh_CSM63718 (SEQ ID NO:9), two nucleotides inserted during T-DNA integration, and the 3’ cotton genomic DNA sequence (SEQ ID NO:12).

[0072] SEQ ID NO:11 is a 1,000-nucleotide sequence representing the 5’ flanking cotton genomic DNA up to the transgene insert (SEQ ID NO:9). SEQ ID NO:11 corresponds to nucleotide positions 1-1,000 of SEQ ID NO:10.

[0073] SEQ ID NO:12 is a 1,000-nucleotide sequence representing the 3’ flanking cotton genomic DNA after the transgene insert (SEQ ID NO:9). SEQ ID NO:12 corresponds to nucleotide positions 17,737-18,736 of SEQ ID NO:10.

[0074] SEQ ID NO:13 is a 2,103-nucleotide sequence representing wildtype cotton genomic DNA at the location where the transgenic sequence (SEQ ID NO:9) was inserted in event Gh_CSM63718. A 103-nucleotide fragment of SEQ ID NO:13 (nucleotides 1,001-1,103) was deleted in event Gh_CSM63718 due to insertion of the T-DNA. .

[0075] SEQ ID NO:14 is a 5,000-nucleotide sequence representing cotton genomic DNA that flanks the transgenic insert at the 5’ end of the insert. The sequence is based on the genomic sequence of cotton TM-1 germplasm.

[0076] SEQ ID NO:15 is a 5,000-nucleotide sequence representing cotton genomic DNA that flanks the transgenic insert at the 3’ end of the insert. The sequence is based on the genomic sequence of cotton TM-1 germplasm.

[0077] SEQ ID NO:16 is a 23-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ22496 used as an internal control for the event assay for cotton event Gh_CSM63718 and hybridizes to a region of the cotton genome.

[0078] SEQ ID NO:17 is a 19-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ22497 used as an internal control for the event assay for cotton event Gh_CSM63718 and hybridizes to a region of the cotton genome. 30 US_ACTIVE\130153301\V-1

[0079] SEQ ID NO:18 is a 14-nucleotide sequence corresponding to a VIC-MGB probe referred to as PB50562 used as an internal control for the event assay for cotton event Gh_CSM63718 and hybridizes to a region of the cotton genome.

[0080] SEQ ID NO:19 is a 24-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51787 used in event-specific assay and zygosity assay to detect cotton event Gh_CSM63718 DNA in a sample and is the reverse complement of the nucleotide sequence corresponding to positions 17,737-17,760 of SEQ ID NO:10.

[0081] SEQ ID NO:20 is a 22-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51702 used in event-specific assay and zygosity assay to detect cotton event Gh_CSM63718 DNA in a sample and is identical to the nucleotide sequence corresponding to positions 17,649-17,670 of SEQ ID NO:10.

[0082] SEQ ID NO:21 is an 18-nucleotide sequence corresponding to a 6FAM-MGB probe referred to as PB50308 used in event-specific assay and zygosity assay to detect cotton event Gh_CSM63718 DNA in a sample and is identical to the reverse complement of the nucleotide sequence corresponding to positions 17,676-17,693 of SEQ ID NO:10.

[0083] SEQ ID NO:22 is a 31-nucleotide sequence corresponding to a thermal amplification forward primer referred to as SQ52074 used in zygosity assay for detection of the wild-type (WT) allele DNA in a sample and hybridizes to a region of the cotton genome.

[0084] SEQ ID NO:23 is an 18-nucleotide sequence corresponding to a VIC-MGB probe referred to as PB50683 used in a zygosity assay for detection of the WT allele DNA in a sample and hybridizes to a region of the cotton genome.

[0085] SEQ ID NOs:24-57 are the nucleotide sequences for the genetic elements in the transgenic insert of cotton event Gh_CSM63718 and are further described in Table 1 hereinbelow.

[0086] SEQ ID NOs:58–127 are 50-nucleotide sequences in the 5’ flank genomic sequence of event Gh_CSM63718. SEQ ID NOs:58–77 are based on the genomic sequence of the transformation germplasm; SEQ ID NOs:78–127 are based on the genomic sequence of cotton TM-1 germplasm.

[0087] SEQ ID NOs:128–197 are 50-nucleotide sequences in the 3’ flank genomic sequence of event Gh_CSM63718. SEQ ID NOs:128–147 are based on the genomic sequence of the 31 US_ACTIVE\130153301\V-1transformation germplasm; SEQ ID NOs:148–197 are based on the genomic sequence of cotton TM-1 germplasm.

[0088] SEQ ID NOs:198 and 199 are the nucleotide and amino acid sequences of LbCas12a (also known as LbCpf1) of Lachnospiraceae bacterium ND2006, respectively.

[0089] SEQ ID NO:200 is the amino acid sequence for LbCas12a_V1 (G532R / K595R).

[0090] SEQ ID NO:201 is the amino acid sequence for LbCas12a_V2 (G532R / K538V / Y542R).

[0091] SEQ ID NO:202 is the amino acid sequence for Cas12a of Francisella_novicida (FnCas12a).

[0092] SEQ ID NO:203 is the nucleotide sequence for the gRNA repeat for LbCas12a.

[0093] SEQ ID NO:204 is the nucleotide sequence for the gRNA repeat for FnCas12a.

[0094] SEQ ID NO:205 is the nucleotide sequence for the gRNA gRNA_5F-87.

[0095] SEQ ID NO:206 is the nucleotide sequence for the gRNA gRNA_3F-45.

[0096] SED ID NO:207 is the amino acid sequence for the PAT protein.

[0097] SED ID NO:208 is the amino acid sequence for the TDO protein.

[0098] SED ID NO:209 is the amino acid sequence for the DMO protein.

[0099] SED ID NO:210 is the amino acid sequence for the CP4 EPSPS protein.

[0100] SED ID NO:211 is the amino acid sequence for the PPO protein.

[0101] SEQ ID NO:212 is a 17,129-nucleotide sequence corresponding to the contig nucleotide sequence of the 5´ genomic flanking DNA nucleotide sequence, the inserted T-DNA nucleotide sequence in cotton event GH_BCS246002, and the 3´ genomic flanking DNA nucleotide sequence.

[0102] SEQ ID NO:213 is a 15,129-nucleotide sequence corresponding to the transgenic inserted T-DNA of cotton event GH_BCS246002.

[0103] SEQ ID NO:214 is a 50-nucleotide sequence representing the 5´ junction region of cotton genomic DNA and the integrated transgenic expression cassette in cotton event GH_BCS246002.

[0104] SEQ ID NO:215 is a 100-nucleotide sequence representing the 5´ junction region of cotton genomic DNA and the integrated transgenic expression cassette in cotton event GH_BCS246002. 32 US_ACTIVE\130153301\V-1

[0105] SEQ ID NO:216 is a 200-nucleotide sequence representing the 5´ junction region of cotton genomic DNA and the integrated transgenic expression cassette in cotton event GH_BCS246002.

[0106] SEQ ID NO:217 is a 1,200-nucleotide sequence representing the 5´ junction region of cotton genomic DNA and the integrated transgenic expression cassette in cotton event GH_BCS246002.

[0107] SEQ ID NO:218 is a 50-nucleotide sequence representing the 3´ junction region of integrated transgenic expression cassette in cotton event GH_BCS246002.

[0108] SEQ ID NO:219 is a 100-nucleotide sequence representing the 3´ junction region of integrated transgenic expression cassette in cotton event GH_BCS246002.

[0109] SEQ ID NO:220 is a 200-nucleotide sequence representing the 3´ junction region of integrated transgenic expression cassette in cotton event GH_BCS246002.

[0110] SEQ ID NO:221 is a 1,200-nucleotide sequence representing the 3´ junction region of integrated transgenic expression cassette in cotton event GH_BCS246002.

[0111] SEQ ID NO:222 is a 1,000-nucleotide sequence representing the 5´ flanking cotton genomic DNA up to the inserted T-DNA in cotton event GH_BCS246002.

[0112] SEQ ID NO:223 is a 1,000-nucleotide sequence representing the 3´ flanking cotton genomic DNA after the inserted T-DNA in cotton event GH_BCS246002.

[0113] SEQ ID NOs:224 and 225 are the nucleotide and amino acid sequences of Cry1B.3, a chimeric insect toxin comprised of domains 1 and 2 of Cry1Be, domain 3 of Cry1Ka, and a protoxin domain of Cry1Be.

[0114] SEQ ID NOs:226 and 227 are the nucleotide and amino acid sequences of Cry1Da_7, a Cry1Da insect toxin with amino acid modifications to improve efficacy.

[0115] SEQ ID NOs:228 and 229 are the nucleotide and amino acid sequences of Vip3Cb1.1, a VipCb1 insect toxin.

[0116] SEQ ID NOs:230 and 231 are the nucleotide and amino acid sequences of Cry2Ab.

[0117] SEQ ID NO:232 is a 2,267-nucleotide sequence representing 1,877 nucleotides of the 5’ flank region of the cotton genomic DNA and 390 nucleotides of the integrated transgene insert in cotton event MON15947. 33 US_ACTIVE\130153301\V-1

[0118] SEQ ID NO:233 is a 1,360-nucleotide sequence representing 349 nucleotides of the integrated transgene insert and 1,012 nucleotides of the 3’ flank region of the cotton genomic DNA in cotton event MON15947.

[0119] SEQ ID NO:234 is a 23-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51334 used in an element-specific assay to detect PPO_H_N90 DNA in a sample and is the reverse complement of the nucleotide sequence corresponding to positions 295– 317 of SEQ ID NO:50.

[0120] SEQ ID NO:235 is a 19-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51335 used in an element-specific assay to detect PPO_H_N90 DNA in a sample and is identical to the nucleotide sequence corresponding to positions 174–192 of SEQ ID NO:50.

[0121] SEQ ID NO:236 is a 19-nucleotide sequence corresponding to a 6FAM-MGB probe referred to as PB50535 used in an element-specific assay to detect PPO_H_N90 DNA in a sample and is identical to the reverse complement of the nucleotide sequence corresponding to positions 319–337 of SEQ ID NO:50.

[0122] SEQ ID NO:237 is a 20-nucleotide sequence corresponding to a VIC-MGB probe referred to as PB13032 used as an internal control for the element-specific assay for detection of PPO_H_N90 and hybridizes to a region of the cotton genome. DETAILED DESCRIPTION

[0123] The following definitions, descriptions, and methods are provided to better define the invention and to guide those of ordinary skill in the art in the practice of the invention. Unless otherwise noted, terms are to be understood according to conventional usage by those of ordinary skill in the relevant art.

[0124] Herbicide tolerance is an important agronomic trait for effective weed control to maintain favorable crop growing conditions and crop yields and is achieved by engineering of herbicide tolerance transgenes in crop plants using modern plant biotechnology techniques. Cotton event Gh_CSM63718 confers tolerance to glufosinate, ß-triketone HPPD inhibitors, dicamba, 34 US_ACTIVE\130153301\V-1glyphosate, PPO herbicides, and any combination thereof and provides multiple modes of action for weed control and herbicide-resistant weed management.

[0125] Cotton event Gh_CSM63718 is provided. The event Gh_CSM63718 was produced by Agrobacterium-mediated transformation of cotton seed-derived embryo explants with a DNA construct comprising seven transgene expression cassettes: 1) a PAT expression cassette for conferring tolerance to glufosinate, comprising in operable linkage i) a ribulose bisphosphate carboxylase / oxygenase (RuBisCO) activase gene promoter, and a leader sequence from Arabidopsis thaliana, ii) a codon-optimized phosphinothricin N-acetyltransferase (PAT) coding sequence from Streptomyces viridochromogenes, and iii) a 3’ untranslated region (UTR) of a small heat shock protein (Hsp20) from Medicago truncatula; 2) a TDO expression cassette for conferring tolerance to ß-triketone HPPD inhibitor herbicides, comprising in operable linkage i) an enhancer from the strawberry vein banding virus (SVBV) fused to the promoter and 5’ UTR from a chlorophyll A / B binding protein (CAB1) gene from Cucumis melo, ii) a codon-optimized triketone dioxygenase (TDO, also known as HIS1) coding sequence from Oryza sativa, and iii) a 3’ UTR of a translation machinery associated 7 protein (TMA7) from Medicago truncatula; 3) a DMO expression cassette for conferring tolerance to dicamba, comprising in operable linkage i) a polyubiquitin gene (UBQ10) promoter, a leader and an intron sequence from Arabidopsis thaliana, ii) an N-terminal chloroplast transit peptide coding sequence of albino and pale green (APG6) from Arabidopsis thaliana fused to a codon-optimized dicamba monooxygenase coding sequence from Stenotrophomonas maltophilia; and iii) a 3’ UTR of an aluminum-induced Sali3-2 protein from Medicago truncatula; 4) an CP4 EPSPS (also known as CP4) expression cassette for conferring tolerance to glyphosate, comprising in operable linkage i) an enhancer of the 35S gene from Figwort Mosaic Virus (FMV), ii) a promoter, a leader sequence, and an intron sequence of the elongation factor 1A gene (ELF1a) from Arabidopsis thaliana, iii) an N-terminal chloroplast transit peptide of granule bound starch synthase I from Triticum aestivum fused to a codon optimized 5-enolpyruvylshikimate-3-phosphate synthase gene (EPSPS) from Agrobacterium sp. strain CP4, and iv) a 3’ UTR of a ribulose 1,5-bisphosphate carboxylase small subunit E9 (rbcS- E9) gene from Pisum sativum; 5) a PPO expression cassette for conferring tolerance to PPO herbicides, comprising in operable linkage i) an enhancer derived from multiple enhancer sequences from Arabidopsis thaliana, ii) a promoter sequence designed from multiple promoter 35 US_ACTIVE\130153301\V-1sequences from Arabidopsis thaliana, iii) an intron and 5’ UTR for a cytochrome C oxidase subunit VIa gene from Arabidopsis thaliana fused a 5’ UTR designed from multiple 5’ UTR sequences from Arabidopsis thaliana, iv) an N-terminal chloroplast transit peptide coding sequence of APG6 from Arabidopsis thaliana, with monocot codon usage fused to the coding region of a protoporphyrinogen oxidase gene from Enterobacter cloacae with codons optimized for cotton, and v) a 3’ UTR from the fiber FbLate-2 gene from Gossypium barbadense; 6) an aadA expression cassette as a selectable marker for selection of transgenic events, comprising in operable linkage i) a promoter, 5 untranslated region and intron sequences of the actin 7 gene (Act7) from Arabidopsis thaliana, ii) an N-terminal chloroplast transit peptide from 5-enolpyruvylshikimate- 3-phosphate synthase (EPSPS) from Arabidopsis thaliana, fused to the coding region of the Tn7 adenylyltransferase from Escherichia coli, and iii) a 3’ UTR of the nopaline synthase (NOS) gene from Agrobacterium tumefaciens; 7) a Cre expression cassette for removal of the aadA and Cre cassettes flanked by two Lox sites, comprising in operable linkage i) a promoter and 5’ UTR of an anther specific LAT52 gene from Solanum lycopersicum, ii) a coding region of CRE recombinase from Enterobacteria phage P1, inserted internally with the second intron from the light inducible gene (LS1) from Solanum tuberosum for minimized bacterial expression, iii) a 3’ UTR of a cell division cycle 45 (CDC45) gene from Arabidopsis thaliana.

[0126] Plant transformation techniques, such as Agrobacterium-mediated or biolistic transformation, can be used to insert foreign DNA (also known as transgenic DNA) randomly or by site directed insertion (also known as targeted insertion) into a chromosome in a plant cell to produce a genetically engineered plant cell, also referred to as a “transgenic” or “recombinant” cell. Using these transformation techniques, many individual cells can be transformed, each resulting in a unique “transgenic event” or “event” due to insertion of the foreign DNA into the genome. A transgenic plant can then be regenerated from each individual transgenic cell. This results in every cell of the transgenic plant containing the uniquely inserted transgenic event as a stable part of its genome. The transgenic plant can then be used to produce progeny plants, each containing the unique transgenic event. The term “transgenic” refers to a plant, plant part, plant cell, seed, progeny plant, or DNA molecule, construct, or sequence comprising a transgene – e.g., a “transgenic cell” refers to a cell comprising a transgene. 36 US_ACTIVE\130153301\V-1

[0127] Cotton event Gh_CSM63718 was produced and identified by a complex research and development process. This process included: (i) design and selection of DNA constructs comprising the seven transgene cassettes based on design and testing of individual transgene cassettes with combinations of different expression elements; (ii) transformation of thousands of cotton cells with the DNA constructs; (iii) regeneration of a large population of transgenic events; and (iv) rigorous multi-year construct and event selection involving molecular characterization of the large number of transgenic events, greenhouse and field trials for herbicide tolerance efficacy and agronomic performance at different locations and in different geographies. Cotton event Gh_CSM63718 was thus produced and selected as a uniquely superior event useful for broad-scale agronomic commercial purposes. Figure 3 illustrates the approximate timelines for the research, testing and development, leading to selection of the commercial event cotton event Gh_CSM63718.

[0128] Detailed molecular characterization was conducted on transgenic events. Event Gh_CSM63718 was selected based on stringent molecular criteria, as well as other selection criteria such as herbicide tolerance efficacy and agronomic performance. The results from such molecular analyses confirmed that: (1) event Gh_CSM63718 contains one copy of the inserted T- DNA at a single genomic location; (2) only the PAT, TDO, DMO, EPSPS and PPO expression cassettes and one lox site between the left and right borders of the T-DNA are present in the event, and no additional element from the transformation construct was present, such as the transformation construct backbone sequence or the aadA or Cre cassette, or the ISR1 or fragment(s); (3) the transgenic DNA was inserted in an intergenic region, far away from any endogenous genes or repeat regions; and (4) the transgenic event produced the correct sized transcripts and proteins for the PAT, TDO, DMO, EPSPS and PPO transgenes, verified by Northern hybridization and Western hybridization analyses, respectively. Furthermore, DNA sequence analyses and protein expression assays were performed to: (1) determine the 5’ and 3’ transgenic insert-to-plant genome junctions; (2) confirm the organization of the transgene cassettes and elements within the insert; (3) verify the complete nucleotide sequence of the inserted transgenic DNA (SEQ ID NO:9); and (4) determine the PAT, TDO, DMO, EPSPS and PPO protein levels in different tissues such as leaf and seed over multiple generations. In addition, primers and probes were designed, and thermal amplification assays were developed and verified 37 US_ACTIVE\130153301\V-1for producing specific amplicons diagnostic for the presence of event Gh_CSM63718 in a sample. As used herein, the 5’ and 3’ designations in reference to the junction, direction and site of the transgenic event insertion is relative to the left border to right border direction of the inserted T- DNA, with the 5’ junction and genomic sequence being upstream of the left border and transgene, and the 3’ junction and genomic sequence being downstream of the right border and transgene.

[0129] As used herein, an “expression cassette” or “cassette” or “transgene cassette” is a recombinant DNA molecule or sequence comprising a combination of distinct elements for expressing an RNA and / or protein encoded by the coding sequence of a transgene in a transformed plant cell or transformed plant comprising the transgene. As provided herein, an “expression cassette” or “cassette” or “transgene cassette” includes one or more regulatory element(s) operably linked to a coding or transcribable DNA sequence. The regulatory elements can include a promoter, a leader, a 5’ untranslated region (5’ UTR), an intron and / or a 3’ untranslated region (3’ UTR). The “expression cassette” or “cassette” or “transgene cassette” is recombinant and heterologous with respect to the transformed plant cell genome, as well as to the combination of the different genetic elements. For purposes of the present disclosure, such an “expression cassette” or “cassette” or “transgene cassette” is a recombinant DNA molecule or sequence that encodes a protein for conferring tolerance to at least one class of herbicides as described herein. Table 1 provides a list of the genetic elements contained in the transgene cassettes in the transgenic insert (SEQ ID NO:9) of cotton event Gh_CSM63718.

[0130] Insertion of the transgenic DNA into the genome of the cotton plant is accomplished by plant transformation methods known in the art and creates a new transgenic genomic DNA sequence, known as a “transgenic event” or an “event” or a “transgenic event locus.” The DNA sequence of the event consists of the inserted foreign DNA (referred to as the “transgenic insert”) and the genomic DNA adjacent to, or “flanking” the transgenic insert on either side of the insertion location. As used herein, the term “flanking” in reference to a transgenic event refers to the plant genomic sequence(s) adjacent to the transgenic DNA insertion in the genome of the transformed plant, plant part, plant tissue, or plant cell comprising the transgenic event on the 5’ and / or 3’ end(s) of the transgenic event insertion. Likewise, “flanking DNA” refers to a length of genomic DNA sequence adjacent to the transgenic DNA insertion in the genome of the transformed event on the 5’ and / or 3’ end(s) of the insertion. A “5’ flank”, therefore, means the cotton genomic DNA 38 US_ACTIVE\130153301\V-1sequence adjacent to and upstream (or on the 5’ end) of the transgenic DNA insertion. For example, a “5’ flank” can include the cotton genomic DNA sequence immediately adjacent to and upstream (on the 5’ end) of the transgenic insertion, or any cotton genomic DNA sequence upstream (on the 5’ end) of the transgenic insertion that is not immediately adjacent to the transgenic insertion but is within about 5000 nucleotides, within about 3000 nucleotides, or within about 1000 nucleotides upstream of the transgenic insertion. Likewise, a “3’ flank” means the cotton genomic DNA sequence adjacent to and downstream (or on the 3’ end) of the transgenic insert. For example, a “3’ flank” can include the cotton genomic DNA sequence immediately adjacent to and downstream (on the 3’ end) of the transgenic insertion, or any cotton genomic DNA sequence downstream (on the 3’ end) of the transgenic insertion that is not immediately adjacent to the transgenic insertion but is within about 5000 nucleotides, within about 3000 nucleotides, or within about 1000 nucleotides downstream of the transgenic insertion. The DNA sequence of an event is unique to and specific for the event and can be readily identified when compared to other DNA sequences, such as that of other events or untransformed cotton genomic DNA. Cotton event Gh_CSM63718 has the new and unique DNA sequence provided as SEQ ID NO:10, which comprises a contiguous sequence comprising the 5’ cotton genomic flanking sequence provided as SEQ ID NO:11, the transgenic insert sequence provided as SEQ ID NO:9, and the 3’ cotton genomic flanking sequence provided as SEQ ID NO:12 (Figure 1). Cotton event Gh_CSM63718 is thus a DNA molecule that is an integral part of the chromosome of transgenic cotton cells and plants comprising the event and as such is static and may be passed on to progeny cells and plants. As is described further in the Examples hereinbelow, various gene editing tools exist that would permit modification of the transgenic insert and / or the flanking genomic DNA of cotton event Gh_CSM63718, such as by deletion, insertion, transposition, or substitution of nucleic acid sequence(s); the event is still uniquely characterized by the presence of heterologous DNA at the particular position in the genome occupied by cotton event Gh_CSM63718 relative to flanking portions of the native cotton genome. 39 US_ACTIVE\130153301\V-1Table 1. Elements and Description of Cotton Event Gh_CSM63718 Element SEQ Position in Description ID SEQ ID 1 ’ , s es .40 US_ACTIVE\130153301\V-1Element SEQ Position in Description ID SEQ ID NO NO 10 c m.41 US_ACTIVE\130153301\V-1Element SEQ Position in Description ID SEQ ID NO NO 10 ’

[0131] Progeny of the original transformed cell and plant that comprise cotton event Gh_CSM63718 are provided. Such progeny may be produced by selfing of a cotton plant comprising cotton event Gh_CSM63718, or by sexual cross or outcrossing between a cotton plant comprising cotton event Gh_CSM63718 and another plant that does or does not contain the event, or by any other method known in the art including any plant cell or tissue culture method, wherein the progeny includes the cotton event Gh_CSM63718. The other plant may be a transgenic plant comprising the same and / or different event(s) or may be a non-transgenic plant, and each parental plant in a cross or outcross may be the same or different germplasm or breeding line. Cotton event 42 US_ACTIVE\130153301\V-1Gh_CSM63718 is passed from the original parent through each generation to the progeny. A “transgenic plant” or “plant”, therefore, can be the original transformant plant regenerated from the transformed plant cell and comprising the transgenic DNA and event, or a progeny plant of the original transformant plant, which may be separated from the transformant by one or more generations, that retains the transgenic DNA and event at the same specific location and sequence context in the plant’s genome. The transformant or progeny plant may be homozygous or heterozygous for event Gh_CSM63718. In addition, a “transgenic plant” may comprise a plant having the transgenes stably inserted into the genome of at least one cell of the plant (i.e., cotton event Gh_CSM63718 in at least one cell of the plant), and the plant may be chimeric or non- chimeric with respect to the transgenes and / or event. A transgenic plant is chimeric with respect to a transgene if not all cells of the plant comprise the transgenes.

[0132] The present disclosure describes introduction of event Gh_CSM63718 into cotton, and thus the term “cotton event Gh_CSM63718” is used to refer to the event herein. However, those of skill in the art will understand that event Gh_CSM63718 could be introduced into other varieties or related cotton species by crosses, such as Gossypium hirsutum (also known as upland cotton), Gossypium barbadense (also known as extra-long staple cotton), Gossypium arboretum (also known as tree cotton), Gossypium herbaceum (also known as levant cotton), and other Gossypium species.

[0133] Cotton event Gh_CSM63718 provides to cotton cells, plants, plant parts, seeds and progeny that comprise the event tolerance to inhibitors of glutamine synthetase such as glufosinate, ß-triketone HPPD inhibitors, benzoic acid auxin herbicides such as dicamba, EPSPS inhibitors such as glyphosate, PPO herbicides, and any combination thereof. The term “ß-triketone HPPD inhibitor(s)” and “ß-triketone HPPD inhibitor herbicide(s)” are used interchangeably herein and refer to chemical agents that target and inhibit the enzymatic activity of triketone dioxygenase. Cotton event Gh_CSM63718 provides tolerance to various “ß-triketone HPPD inhibitors, including, but not limited to, mesotrione, benzobicyclon (BBC), tembotrione, sulcotrione, tefuryltrione, and any combination thereof. The terms “PPO herbicide”, “PPO inhibitor”, and “PPO-inhibiting herbicide” are used interchangeably herein and refer to chemical agents that target and inhibit the enzymatic activity of a protoporphyrinogen oxidase (PPO). Cotton event Gh_CSM63718 provides tolerance to various PPO herbicides, including, but not limited to, 43 US_ACTIVE\130153301\V-1flumioxazin, epyrifenacil (also referred to as S-3100 or rapidicil; IUPAC name: ethyl [(3-{2- chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidin-1(2H)-yl]-4- fluorophenoxy}-2-pyridyl)oxy]acetate), lactofen, acifluorfen, pyraflufen, pyraflufen-ethyl, oxadiazon, butafenacil, carfentrazone-ethyl, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2- yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2- methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}pyridin-2-yl)oxy]acetate, cyclopropylmethyl (2-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}phenoxy)acetate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoximacil), fomesafen, saflufenacil, sulfentrazone, tiafenacil, and trifludimoxazin, 1-ethoxy-1-oxopropan-2- yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy- 2-methyl-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, and 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro- 5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- 44 US_ACTIVE\130153301\V-1fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, ethyl 1-({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate.

[0134] Cotton event Gh_CSM63718 is characterized as a single copy insertion into one locus in the cotton genome, resulting in two new loci or junction sequences (e.g., the sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8) spanning portions of the inserted DNA and the cotton genomic DNA that are not known to appear or exist naturally in the cotton genome or other transgenic cotton events, i.e., they are unique to event Gh_CSM63718. SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7 span the 5’ junction of the cotton genomic sequence and the transgenic DNA insert, and SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8 span the 3’ junction. These junction sequences are useful in detecting the presence of the event Gh_CSM63718 in cotton cells, seed, plants, plant parts, progeny, and plant products, such as cotton commodity products. Polynucleotide or DNA molecular probes and / or primer pairs are described herein for use in identifying the presence of these various junction sequences in biological samples containing or derived from, or suspected of containing or being derived from, cotton cells, seeds, plants, plant parts, progeny, or commodity products that contain the event Gh_CSM63718. 45 US_ACTIVE\130153301\V-1

[0135] As used herein, the term “derived” or “derived from” in reference to a particular DNA molecule, amplicon or sequence in relation to a cotton plant, plant part, seed, progeny, cell and / or cotton plant product, such as a commodity product, means that the DNA molecule, amplicon or sequence is taken, purified, isolated, or made, directly or indirectly, from such cotton plant, plant part, seed, progeny, cell and / or cotton plant product, such as a commodity product. Alternatively, the term “derived” or “derived from” in reference to a cotton plant product, such as a commodity product, in relation to cotton plant, plant part, seed, progeny, or cell, means that the cotton plant product is taken, purified, isolated, or made, directly or indirectly, from such cotton plant, plant part, seed, progeny, or cell.

[0136] “Capable of being detected” refers to the ability of a particular DNA molecule, segment or sequence to be detected in a sample, such as by amplification and determining its presence, size or sequence such as by DNA sequence analysis, and / or binding of a probe to the target DNA molecule, segment or sequence.

[0137] A “sample” is intended to refer to any composition comprising or derived from, either directly or indirectly, a biological sample, source, or material. The sample may generally comprise cotton DNA and / or substantially or completely pure, purified, or isolated cotton DNA. A “biological sample” contains biological materials, including but not limited to DNA obtained or derived from, either directly or indirectly, the genome of a cotton cell(s), tissue(s), seed(s), plant(s), plant part(s) and / or cotton plant product(s), such as a commodity product(s). Such cotton cell(s), tissue(s), seed(s), plant(s), plant part(s) and / or cotton plant product(s), such as a commodity product(s), may comprise cotton event Gh_CSM63718, or DNA molecule(s) and / or DNA segment(s) comprising cotton event Gh_CSM63718. In some embodiments, a sample or biological sample may comprise cotton cell(s), cotton tissue(s), cotton seed(s), cotton plant(s), cotton plant part(s) and / or cotton plant product(s), whose cells or cellular membranes have been fractured (e.g., disrupted or opened) to release the contents of the cotton cell(s) including genomic DNA or proteins and / or make the contents of the cotton cell(s) including genomic DNA or proteins accessible or usable for assays or testing. “Directly” refers to directly obtaining DNA by a skilled artisan from the cotton genome by fracturing cotton cells (or by obtaining samples of cotton that contain fractured cotton cells) and exposing or using the genomic DNA or protein from cotton cells for the purposes of detection. “Indirectly” refers to obtaining by a skilled artisan a target or 46 US_ACTIVE\130153301\V-1specific reference DNA (e.g., a novel and unique junction segment(s) described herein as being diagnostic for the presence of the event Gh_CSM63718) in a particular sample, by means other than by obtaining directly via fracturing of cotton cells or obtaining a sample of cotton that contains fractured cotton cells. Such indirect means include, but are not limited to, amplification of a DNA segment that contains a DNA sequence targeted by a particular probe(s) and / or primer set(s) designed to bind with specificity to or near the target sequence, or amplification of a DNA segment comprising all or part of a target sequence that can be measured and characterized (e.g., measured by migration or separation from other segments of DNA and / or identification in an effective matrix, such as an agarose or acrylamide gel or the like, or characterized by direct sequence analysis of the amplicon(s), or cloning of the amplicon(s) into a vector(s) and direct sequencing of the inserted amplicon(s) present within such vector(s)).

[0138] As used herein, the term “recombinant” refers to a non-naturally occurring DNA, protein, combination, or organism that would not normally be found or exist in nature and is created by human intervention. As used herein, a “recombinant DNA molecule” is a DNA molecule comprising a combination of DNA molecules that would not naturally occur together and is the result of human intervention. Two or more elements of such combination of DNA sequences may be operably linked to one another. For example, a recombinant DNA molecule may comprise a combination of at least two DNA molecules heterologous with respect to each other, such as a DNA molecule that comprises a coding sequence operably linked to a heterologous promoter and / or other regulatory expression element(s), and / or a transgene and a heterologous plant genomic DNA adjacent to the transgene, and / or a DNA molecule that is artificially synthesized and comprises a polynucleotide sequence that deviates from any polynucleotide sequence that would normally exist in nature. A recombinant DNA molecule may comprise all or part of a junction sequence of the genome of the event and all or part of the transgenic insert of the genome of the event, and / or may comprise a recombinant or heterologous DNA fragment of cotton event Gh_CSM63718. Examples of recombinant DNA molecules include a DNA molecule comprising at least one polynucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10; a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 47 US_ACTIVE\130153301\V-196%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9; and a complete complement of any of the foregoing. Such recombinant DNA molecules can be derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product comprising cotton event Gh_CSM63718. Alternatively, such recombinant DNA molecules can be comprised in a cotton plant, seed, plant part, plant cell, or progeny plant comprising cotton event Gh_CSM63718, or a commodity product produced therefrom. A representative sample of seed comprising cotton event Gh_CSM63718 has been deposited as ATCC Accession No. PTA-127638. Such recombinant DNA molecules can be formed by the insertion of a heterologous nucleic acid molecule into the genomic DNA of a cotton plant or cotton cell. Such recombinant DNA molecules can be an amplicon diagnostic for the presence of cotton event Gh_CSM63718.

[0139] As used herein, a “recombinant” in reference to a plant, plant part, seed, plant cell, or progeny is a plant, plant part, seed, plant cell or progeny that would not normally exist in nature, is the result of human intervention, and contains a transgenic DNA molecule stably integrated into the genome of the plant, plant part, seed, plant cell, or progeny. As a result of such genomic insertion, the recombinant or transgenic plant, plant part, seed, plant cell, or progeny is something new and distinctly different from any related wildtype or naturally occurring plant, plant part, seed, plant cell or progeny. An example of a recombinant plant is a cotton plant containing the cotton event Gh_CSM63718.

[0140] As used herein, the term “transgene” refers to a DNA molecule artificially incorporated into an organism’s genome as a result of human intervention, such as by plant transformation methods. A transgene may be heterologous to the organism. The term “transgenic insert” as used herein refers to the foreign or heterologous DNA inserted by plant transformation techniques into the cotton genome to produce cotton event Gh_CSM63718. The sequence for the transgenic insert of cotton event Gh_CSM63718 is provided as SEQ ID NO:9.

[0141] As used herein, the term “heterologous” in reference to a combination of two or more DNA sequences or elements means that the two or more DNA sequences or elements do not normally exist together as such combination in nature without human intervention. For example, a DNA molecule may be from a first species or a recombinant DNA molecule and inserted into 48 US_ACTIVE\130153301\V-1the genome of a second species. The DNA molecule would thus be heterologous to the genome and the organism. As used herein, the term “heterologous” in reference to a DNA molecule, construct, sequence or protein in relation to a plant, microorganism, plant cell or plant genome means that the DNA molecule, construct, sequence or protein does not exist in nature as part of such a plant, microorganism, plant cell or plant genome, and / or does not exist in the same physical or genomic location, context or orientation as part of such a plant, microorganism, plant cell or plant genome in nature, without human intervention.

[0142] As used herein, the term “chimeric” refers to a single DNA molecule produced by fusing a first DNA molecule to a second DNA molecule, where neither first nor second DNA molecule would normally be found in that configuration fused to the other. The chimeric DNA molecule is thus a new DNA molecule not normally found in nature. An example of a chimeric DNA molecule is a DNA molecule comprising at least one sequence selected from SEQ ID NO:1- 10.

[0143] As used herein, the term “isolated” in reference to a molecule means that the molecule is at least partially separated from other molecules that are normally associated with it in its native or natural state. In some embodiments, the term “isolated” refers to a DNA molecule that is at least partially separated from the nucleic acids or polynucleotide or DNA sequence(s) that normally flank and are covalently linked to the sequence of the DNA molecule in its native or natural state. An “isolated” DNA molecule may have a DNA sequence corresponding to a portion of the genome of a plant cell without other genomic DNA sequence(s) that normally flank and are covalently linked to the DNA sequence in nature. Such an “isolated” DNA molecule may comprise all or part of a transgene and / or transgenic event, which may comprise all or part of cotton event Gh_CSM63718 or the transgenes or expression cassettes described herein. Nucleic acid sequences or elements, such as a coding sequence, intron sequence, 5’ UTR, promoter sequence, 3’ UTR, and the like, that are naturally found within the DNA of the genome of an organism are not considered to be “isolated” so long as the element is within the genome of the organism and at the location within the genome in which it is naturally found. However, each of these elements, and subparts of these elements, would be “isolated” within the scope of this disclosure so long as the element or subpart is not within the genome of the organism, and at the location within the genome of the organism, in which it is naturally found. An “isolated” DNA molecule may be any 49 US_ACTIVE\130153301\V-1recombinant DNA molecule or amplification product or amplicon, and / or may comprise any DNA sequence removed from its natural or biological state and covalently fused to another DNA molecule or sequence with which it is not associated in nature. Such an isolated DNA molecule could be created by the use of biotechnology techniques, such as by making a recombinant DNA or integrating a foreign or heterologous DNA molecule into the chromosome of a cell, plant, or seed. Thus, any DNA molecule comprising a transgenic, recombinant, chimeric or artificial nucleotide sequence, transgene or expression cassette would be considered to be an “isolated” DNA molecule since these sequences are not naturally occurring, regardless of whether the sequence, transgene or expression cassette is present within a plasmid, vector or construct used to transform plant cells, within the genome of a plant, plant part, plant tissue, plant cell or progeny, or is present in detectable amounts in tissues, progeny, biological samples or commodity products derived from a plant, plant part, plant tissue, progeny or plant cell. A recombinant DNA molecule or sequence, or any fragment derived therefrom, comprising all or part of a transgene or junction sequence of the cotton event Gh_CSM63718 would therefore also be considered to be “isolated.” An “isolated” DNA molecule may be extracted or purified from a transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be present in a homogenate, extract or lysate from any such transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be produced as an amplicon or amplification product from plant genomic DNA and / or extracted or purified DNA from transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or a homogenate, extract or lysate from plant(s), plant part(s), plant cell(s) and / or tissue(s). For the purposes of this disclosure, any transgenic polynucleotide or DNA sequence, i.e., the nucleotide sequence of the DNA inserted into the genome of a plant or bacterium, or present in an extrachromosomal vector, would be considered to be an “isolated” nucleotide or DNA sequence whether it is present within the plasmid or similar structure used to transform the cells, within the genome of the plant or bacterium, or present in detectable amounts in tissues, progeny, biological samples or commodity products derived from the plant or bacterium. An “isolated” DNA molecule is a chemical or biochemical molecule, regardless of whether the molecule is referred to as a nucleic acid, a nucleic acid sequence, a polynucleotide sequence, a DNA sequence, a nucleic acid molecule, a polynucleotide molecule, a DNA molecule, or the like. An “isolated” molecule can provide industrial applicability when present in a plant cell or in a plant genome or when present outside of a plant cell, and 50 US_ACTIVE\130153301\V-1therefore, provides and exhibits (and is intended to provide and exhibit) utility regardless of where the molecule is located.

[0144] As used herein, the term “correspond” or “corresponding”, or the like, when used in the context of a nucleotide position, mutation, insertion and / or substitution in any given polynucleotide (e.g., SEQ ID NO:9) with respect to a reference polynucleotide sequence (e.g., SEQ ID NO:10) refers to the position(s) of the polynucleotide residue(s) in the given sequence that has identity to the residue(s) in the reference nucleotide sequence when the given polynucleotide is aligned to the reference polynucleotide sequence using a global or local sequence alignment algorithm.

[0145] DNA molecules, fragments, and their corresponding DNA sequences, as well as methods of detection are provided. As used herein, the terms “DNA”, “DNA molecule” and “nucleic acid molecule” refer to a deoxyribonucleic acid (DNA) molecule. A DNA molecule may be of genomic or synthetic origin and / or comprise a recombinant or heterologous DNA molecule or sequence. A DNA molecule may be described by convention from the 5’ (upstream) end to the 3’ (downstream) end. As used herein, the term “DNA sequence” refers to the polynucleotide sequence of a DNA molecule, i.e. the sequence of consecutive nucleotides in the DNA molecule. As used herein in reference to nucleotides of a polynucleotide or DNA sequence or molecule, the terms “consecutive” and “contiguous” are interchangeable and synonymous and refer to the 5’ to 3’ order of nucleotides in a polynucleotide or DNA sequence, strand or molecule without any gap or interruption between them. The nomenclature used is that required by Title 37 of the United States Code of Federal Regulations § 1.822 and set forth in the tables in WIPO Standard ST.25 (1998), Appendix 2, Tables 1 and 3. By convention, DNA sequences and fragments thereof are disclosed with reference to the 5’ to 3’ direction of only one strand of the two complementary DNA sequence strands of a DNA molecule. By implication and intent, the complementary sequences of the sequences provided here (the sequences of the complementary strand), also referred to in the art as the reverse complementary or reverse complement sequences, are within the scope of the present disclosure and are expressly intended to be within the scope of the subject matter claimed. As used herein references to SEQ ID NOs:1-10 and fragments thereof include and refer to the sequence of the complementary strand and fragments thereof. 51 US_ACTIVE\130153301\V-1

[0146] Also provided is a nucleic acid molecule comprising a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to the full length of any one of SEQ ID NOs:1–10.

[0147] For example, a nucleic acid molecule is provided comprising a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to the full length of SEQ ID NO:10 or to the full length of SEQ ID NO: 9.

[0148] A DNA molecule, or a fragment derived therefrom, can also be extracted from plant(s), plant part(s), seed(s), progeny or plant cell(s), or a homogenate, extract or lysate from plant(s), plant part(s), plant cell(s) or seed(s) or progeny, or can be produced as an amplicon from extracted, purified or isolated DNA from plant part(s), plant cell(s) and / or tissue(s), progeny, or a homogenate, extract or lysate from plant(s), plant part(s), plant cell(s), progeny and / or seeds, which may further comprise cotton event Gh_CSM63718.

[0149] As used herein, the term “percent sequence identity” or “% sequence identity” refers to the percentage of identical nucleotides or amino acids in a linear polynucleotide or polypeptide sequence of a reference (“query”) sequence (or its complementary strand) as compared to a test (“subject”) sequence (or its complementary strand) when the two sequences are optimally aligned (with appropriate nucleotide or amino acid insertions, deletions, or gaps totaling less than 20 percent of the reference sequence over the window of comparison). Optimal alignment of sequences for aligning a comparison window are well known to those skilled in the art and may be conducted by tools such as the local homology algorithm of Smith and Waterman, the homology alignment algorithm of Needleman and Wunsch, the search for similarity method of Pearson and Lipman, and by computerized implementations of these algorithms such as GAP, BESTFIT, FASTA, and TFASTA available as part of the Sequence Analysis software package of the GCG® Wisconsin Package® (Accelrys Inc., San Diego, Calif.), MEGAlign (DNAStar Inc., 1228 S. Park St., Madison, Wis. 53715), and MUSCLE (version 3.6) (Edgar, “MUSCLE: multiple sequence alignment with high accuracy and high throughput” Nucleic Acids Research 32(5):1792-7 (2004)) 52 US_ACTIVE\130153301\V-1for instance with default parameters. An “identity fraction” for aligned segments of a test sequence and a reference sequence is the number of identical components that are shared by the two aligned sequences divided by the total number of components in the portion of the reference sequence segment being aligned, that is, the entire reference sequence or a smaller defined part of the reference sequence. Percent sequence identity is represented as the identity fraction multiplied by 100. The comparison of one or more sequences may be to a full-length sequence or a portion thereof, or to a longer sequence. Cotton plants, progeny, seeds, cells, plant parts and commodity products comprising a detectable amount of a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9 are within the scope of the present disclosure.

[0150] As used herein, the term “fragment” refers to a smaller piece or sequence of a larger or whole DNA molecule or sequence. For example, a fragment of any one of SEQ ID NOs:1–12 and SEQ ID NOs:14–15 may include a sequence that is at least about 10 consecutive nucleotides, at least about 11 consecutive nucleotides, at least about 12 consecutive nucleotides, at least about 13 consecutive nucleotides, at least about 14 consecutive nucleotides, at least about 15 consecutive nucleotides, at least about 16 consecutive nucleotides, at least about 17 consecutive nucleotides, at least about 18 consecutive nucleotides, at least about 19 consecutive nucleotides, at least about 20 consecutive nucleotides, at least about 21 consecutive nucleotides, at least about 22 consecutive nucleotides, at least about 23 consecutive nucleotides, at least about 24 consecutive nucleotides, at least about 25 consecutive nucleotides, at least about 30 consecutive nucleotides, at least about 35 consecutive nucleotides, at least about 40 consecutive nucleotides, at least about 45 consecutive nucleotides, at least about 50 consecutive nucleotides, at least about 60 consecutive nucleotides, at least about 70 consecutive nucleotides, at least about 80 consecutive nucleotides, at least about 90 consecutive nucleotides, at least about 100 consecutive nucleotides, at least about 150 consecutive nucleotides, at least about 200 consecutive nucleotides, at least about 250 consecutive nucleotides, at least about 300 consecutive nucleotides, at least about 400 consecutive nucleotides, 53 US_ACTIVE\130153301\V-1or at least about 500 consecutive nucleotides of the larger, whole or complete DNA molecule or sequence.

[0151] For example, a “fragment” of the transgenic insert sequence (SEQ ID NO: 9) of cotton event Gh_CSM63718 can comprise at least about 10, at least about 11, at least about 12, at least about 13, at least about 14, at least about 15, at least about 16, at least about 17, at least about 18, at least about 19, at least about 20, at least about 21, at least about 22, at least about 23, at least about 24, at least about 25, at least about 30, at least about 35, at least about 40, at least about 45, at least about 50, at least about 60, at least about 70, at least about 80, at least about 90, at least about 100, at least about 150, at least about 200, at least about 250, at least about 300, at least about 400, or at least about 500 consecutive nucleotides of SEQ ID NO: 9. In addition, the present disclosure encompasses nucleotide sequences that are at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO: 9 or any fragment thereof.

[0152] Similarly, a fragment of the 5’ flank (SEQ ID NO:11 or SEQ ID NO:14) or 3’ flank (SEQ ID NO:12 or SEQ ID NO:15) of cotton event Gh_CSM63718 can comprise at least about 10, at least about 11, at least about 12, at least about 13, at least about 14, at least about 15, at least about 16, at least about 17, at least about 18, at least about 19, at least about 20, at least about 21, at least about 22, at least about 23, at least about 24, at least about 25, at least about 30, at least about 35, at least about 40, at least about 45, at least about 50, at least about 60, at least about 70, at least about 80, at least about 90, at least about 100, at least about 150, at least about 200, at least about 250, at least about 300, at least about 400, or at least about 500 consecutive nucleotides of SEQ ID NO:11 or SEQ ID NO:14; or SEQ ID NO:12 or SEQ ID NO:15. In addition, the present disclosure encompasses nucleotide sequences that are at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:11 or 12, or SEQ ID NO:14 or 15, or any fragment of either thereof.

[0153] As used herein, the term “about” indicates a value or a range of values which would be understood as an equivalent of a stated value and can be greater or lesser than the value or range 54 US_ACTIVE\130153301\V-1of values stated. Each value or range of values preceded by the term “about” is also intended to encompass the embodiment of the stated absolute value or range of values.

[0154] The term “or” is used herein to mean “and / or” unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive. Thus, the term “and / or” as used herein in a phrase such as “X and / or Y” is intended to include “X and Y”, “X or Y”, “X” (alone), and “Y” (alone). Likewise, the term “and / or” as used in a phrase such as “X, Y, and / or Z” is intended to encompass each of the following embodiments: X (alone); Y (alone); Z (alone); X and Y; X and Z; Y and Z; X, Y, and Z; X, Y, or Z; X or Z; Y or Z; Y or Z.

[0155] When used in conjunction with the word “comprising” or other open language, the words “a” and “an” denote “one or more,” unless specifically noted otherwise. The terms “comprise,” “have,” and “include” are open-ended linking verbs. Any forms or tenses of one or more of these verbs, such as “comprises,” “comprising,” “has,” “having,” “includes,” and “including,” are also open-ended. For example, any method that “comprises,” “has,” or “includes” one or more steps is not limited to possessing only those one or more steps and also covers other unlisted steps.

[0156] Cotton event Gh_CSM63718 is characterized as a transgenic insertion into a locus in the cotton genome, resulting in two new junctions (or joining or connection points). The DNA sequence of the region spanning the connection by phosphodiester bond linkage of one end of the transgenic insert to the flanking cotton genomic DNA is referred to herein as a “junction.” In other words, a junction is the connection point or covalent linkage of one end of the transgenic insert and the flanking genomic DNA as one contiguous molecule and is formed by the insertion of a heterologous nucleic acid molecule into the cotton genomic DNA. One junction is found at the 5’ end of the transgenic insert and the other is found at the 3’ end of the transgenic insert, referred to herein as the 5’ and 3’ junctions, respectively. A “junction sequence” refers to a DNA sequence of any length of consecutive nucleotides that spans the 5’ or 3’ junction of a transgenic event in the plant genome. For a “junction sequence” to be specific to a junction between a transgenic event and a flanking genomic sequence, the junction sequence will generally comprise a sufficient number of consecutive nucleotides at one end of the insertion and a sufficient number of consecutive nucleotides of the flanking genomic sequence. According to some embodiments, a “junction sequence” may comprise (i) at least five (5) consecutive nucleotides, at least ten (10) 55 US_ACTIVE\130153301\V-1consecutive nucleotides, at least fifteen (15) consecutive nucleotides, at least twenty (20) consecutive nucleotides, at least twenty five (25) consecutive nucleotides, at least thirty (30) consecutive nucleotides, at least thirty five (35) consecutive nucleotides, at least forty (40) consecutive nucleotides, at least forth five (45) consecutive nucleotides, or at least fifty (50) consecutive nucleotides at one end of the insertion and (ii) at least five (5) consecutive nucleotides, at least ten (10) consecutive nucleotides, at least fifteen (15) consecutive nucleotides, at least twenty (20) consecutive nucleotides, at least twenty five (25) consecutive nucleotides, at least thirty (30) consecutive nucleotides, at least thirty five (35) consecutive nucleotides, at least forty (40) consecutive nucleotides, at least forth five (45) consecutive nucleotides, or at least fifty (50) consecutive nucleotides of the flanking genomic DNA sequence, although it is understood that any length of consecutive nucleotides spanning a junction of a transgenic event in a plant genome may be a junction sequence. Junction sequences of cotton event Gh_CSM63718 are not known to appear or exist naturally in the cotton genome or other transgenic cotton events—they are unique to event Gh_CSM63718, and are apparent to, and a variety of junction sequences of cotton event Gh_CSM63718 can be determined by one of skill in the art using SEQ ID NO:10. In SEQ ID NO:10, the 5’ junction is at nucleotides 1,000–1,001, and the 3’ junction is at nucleotides 17,736–17,737. Illustrative junction sequences of cotton event Gh_CSM63718 are provided as SEQ ID NOs:1–8. Figure 1 illustrates the physical arrangement and locations of the illustrative junction sequences, arranged from 5’ to 3’ (left to right), relative to SEQ ID NO:10. The DNA sequence for the transgenic insert of cotton event Gh_CSM63718 is provided as SEQ ID NO:9. The DNA sequence of the transgenic insert and the cotton genomic DNA flanking each side of the transgenic insert is provided as SEQ ID NO:10. The 5’ junction sequences are provided as SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, and SEQ ID NO:7. The 3’ junction sequences are provided as SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8. The junction sequences of cotton event Gh_CSM63718 may be present as part of the genome of a plant, seed, plant part, progeny or plant cell containing cotton event Gh_CSM63718, or a DNA molecule containing all or part of event Gh_CSM63718. The identification of any one or more of the junction sequences in a DNA molecule or a sample from a plant, plant part, seed, progeny, cell or commodity product indicates that the DNA molecule or plant, plant part, seed, progeny, cell or commodity product contains or comprises event Gh_CSM63718, or was obtained from a cotton plant, plant part, seed, 56 US_ACTIVE\130153301\V-1progeny, cell or commodity product containing or comprising event Gh_CSM63718, and is diagnostic for the presence of cotton event Gh_CSM63718.

[0157] The junction sequences described herein are diagnostic for the presence of all or part of cotton event Gh_CSM63718. Thus, the identification or detection, directly or indirectly, of one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10 in a sample or DNA molecule derived from a cotton plant, plant part, seed, progeny, cell, or a commodity product is diagnostic that the cotton plant, plant part, seed, progeny, cell, or a commodity product has or comprises all or part of cotton event Gh_CSM63718. The identification or detection, directly or indirectly, of a 5’ junction sequence and / or a 3’ junction sequence (each as provided or described herein) in a sample or DNA molecule derived from a cotton plant, plant part, seed, progeny, cell, or a commodity product is diagnostic that the cotton plant, plant part, seed, progeny, cell, or a commodity product has or comprises cotton event Gh_CSM63718. The present disclosure thus provides a DNA molecule that comprises at least one of the nucleotide sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. Any segment of DNA derived from transgenic cotton event Gh_CSM63718 that is sufficient to include at least one of the sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10 is within the scope of the present disclosure. In addition, any DNA or polynucleotide molecule or sequence comprising a sequence complementary to any of the sequences described herein is also within the scope of the present disclosure.

[0158] Polynucleotide molecules are provided, which may be single or double stranded, that can be used either as primers or probes for detecting the presence of DNA comprising all or part of event Gh_CSM63718 in a sample derived from a cotton plant, plant part, seed, progeny, cell, or a commodity product. Such primers or probes are specific for a target polynucleotide sequence and, as such, are useful for the identification of cotton event Gh_CSM63718 polynucleotide by the methods described herein. A primer or probe can hybridize to a target polynucleotide sequence to allow for specific detection or amplification of a polynucleotide molecule that comprises, or is covalently linked and associated with, the target polynucleotide sequence. The primers and / or 57 US_ACTIVE\130153301\V-1probes may be chosen to identify and distinguish detection of a particular transgenic event and not only the presence of a transgene in a plant genome. The target polynucleotide sequence may comprise all or part of cotton event Gh_CSM63718, a junction sequence and / or flanking genomic DNA. Probes and primers according to the present disclosure may have (i) complete or 100% sequence complementarity (i.e., 100% complementary) to a target polynucleotide sequence or (ii) incomplete sequence complementarity to a target polynucleotide, such as at least 60% complementary, at least 65% complementary, at least 70% complementary, at least 75% complementary, at least 80% complementary, at least 85% complementary, at least 90% complementary, at least 95% complementary, or at least 99% complementary to the target polynucleotide sequence as long as the probe or primer has sufficient complementarity to the target polynucleotide sequence to hybridize to the target polynucleotide sequence under stringent hybridization conditions that are suitable and necessary for use of the probe or primer in the relevant amplification or detection assay, reaction or method. As understood in the art, the percentage complementarity of a primer or probe may be lower if the length of the primer or probe is longer and depends on the stringency and use. Provided are illustrative polynucleotide molecules that can be used either as primers or probes for detecting the presence of cotton event Gh_CSM63718 in a sample. Detection of the presence of cotton event Gh_CSM63718 may be done by using methods known in the art, such as thermal or isothermal amplification of nucleic acids or nucleic acid hybridization techniques (such as Northern analysis and Southern analysis).

[0159] A “probe” is a nucleic acid molecule that is complementary to a strand of a target nucleic acid and is useful in hybridization detection methods. Probes include not only deoxyribonucleic or ribonucleic acids but also polyamides and other probe materials that bind specifically to a target DNA sequence and the detection of such binding can be useful in detecting the presence or absence of the target DNA sequence. A probe may be attached to a conventional detectable label or reporter molecule, such as a radioactive isotope, ligand, chemiluminescent agent, or enzyme. Such a probe is complementary to a strand of a target nucleic acid and, in the case of the present disclosure, to a strand of DNA from event Gh_CSM63718 whether from an event Gh_CSM63718-containing plant or from a sample that includes event Gh_CSM63718 DNA.

[0160] Provided herein is a DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization 58 US_ACTIVE\130153301\V-1conditions with cotton event Gh_CSM63718 DNA in a sample, wherein detecting hybridization of the DNA molecule under the stringent hybridization conditions is diagnostic for the presence of cotton event Gh_CSM63718 in the sample. Also provided is a DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe specific for detecting in a sample at least one of: (i) a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718; (ii) a 3’ junction sequence between the transgenic insert of cotton event Gh_CSM63718 and flanking cotton genomic DNA; (iii) SEQ ID NO:9; and (iv) a fragment of SEQ ID NO:9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO:9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718. An illustrative DNA sequence useful as a probe for detecting cotton event Gh_CSM63718 is provided as SEQ ID NO:21. Other DNA sequences useful as probes for detecting cotton event Gh_CSM63718 include SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; and complements of any of the foregoing.

[0161] A “primer” is a DNA molecule or oligonucleotide that is designed for use in specific annealing or hybridization methods that involve an in vitro amplification reaction. A pair of primers may be used with template DNA (such as a sample of cotton event Gh_CSM63718 genomic DNA) in a thermal amplification reaction (such as polymerase chain reaction (PCR)) or any other suitable amplification method known in the art to produce an amplification product or amplicon, where the amplicon produced from such reaction would have a DNA sequence corresponding to sequence of the template DNA located between the two sites where the primers hybridized to the template DNA.

[0162] DNA amplification reactions, methods and techniques are known to those skilled in art. DNA amplification can be accomplished by any of the various nucleic acid amplification methods known in the art, including thermal and isothermal amplification methods such as polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Amplification methods are known in the art and are described, inter alia, in U.S. Patent Nos. 4,683,195 and 4,683,202 and in PCR Protocols: A Guide to Methods and Applications, ed. Innis et al., Academic Press, San Diego, 1990. PCR amplification methods have been developed to amplify up to 22 kb (kilobase) of genomic DNA and up to 42 kb of bacteriophage DNA (Cheng et al., 1994). These 59 US_ACTIVE\130153301\V-1methods as well as other methods known in the art of DNA amplification may be used in the practice of the present disclosure. Examples of DNA amplification methods include PCR, Recombinase Polymerase Amplification (RPA) (see for example U.S. Pat No. 7,485,428), Strand Displacement Amplification (SDA) (see for example, U.S. Pat. Nos. 5,455,166 and 5,470,723), Transcription-Mediated Amplification (TMA) (see for example, Guatelli et al., 1990), Rolling Circle Amplification (RCA) (see for example, Fire and Xu, 1995; Liu, et al., 1996; Lizardi, et al.,1998; U.S. Pat. Nos.5,714,320 and 6,235,502), Helicase Dependent Amplification (HDA) (see for example Vincent et al., 2004; U.S. Pat. No. 7 ,282,328), Multiple Displacement Amplification (MDA) (see for example Dean et al., 2002) and Loop-Mediated Isothermal Amplification (LAMP) (see for example Notomi et al., 2000). A sequence of the heterologous DNA insert and / or flanking genomic DNA sequence from cotton event Gh_CSM63718 can be verified or tested by amplifying such DNA molecules from cotton seed containing event Gh_CSM63718 DNA or cotton plants grown from the cotton seed containing event Gh_CSM63718 DNA, using primers derived from the sequences provided herein, followed by standard DNA sequencing of the PCR amplicon or a cloned DNA fragment thereof.

[0163] As used herein, an “amplification product” or “amplified DNA” or “amplicon” refers to the nucleic acid or DNA molecule or segment produced by a nucleic acid amplification reaction or method as further described herein, which is directed to a target nucleic acid or DNA molecule that is part of a template nucleic acid molecule. Amplification or amplifying refers to making multiple copies of a target DNA molecule or segment from a template DNA. For example, to determine whether a cotton plant, plant part, seed, progeny or plant cell, resulting from selfing or outcross of a parent comprising cotton event Gh_CSM63718 contains cotton event Gh_CSM63718, DNA may be extracted from the cotton plant tissue sample and subjected to an amplification reaction or method using a pair of primers that are specific for a target sequence that is uniquely associated or part of cotton event Gh_CSM63718, such as, for example, a first primer derived from a genomic DNA sequence in the region flanking the heterologous inserted DNA of cotton event Gh_CSM63718 that is elongated by polymerase 5’ to 3’ in the direction of the inserted DNA, and a second primer derived from the heterologous inserted DNA molecule that is elongated by the polymerase 5’ to 3’ in the direction of the flanking genomic DNA from which the first primer is derived. The amplicon may range in length depending on the length of the intervening 60 US_ACTIVE\130153301\V-1polynucleotide or DNA sequence between the two primer target sequences in the template DNA molecule. Alternatively, a primer pair can be derived from the genomic sequence on both sides of the inserted heterologous DNA so as to produce an amplicon that includes the entire insert polynucleotide sequence (e.g., a forward primer targeted to the genomic portion on the 5’ end of SEQ ID NO:10 (i.e. upstream of SEQ ID NO:9) and a reverse primer targeted to the genomic portion on the 3’ end of SEQ ID NO:10 (i.e. downstream of SEQ ID NO:9) that amplifies a DNA molecule comprising the inserted DNA sequence (SEQ ID NO:9) identified herein in the cotton event Gh_CSM63718 genome. The use of the term “amplicon” specifically excludes primer dimers that may be formed in a DNA amplification reaction.

[0164] Provided herein is a pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein the first and the second DNA molecules are different from one another and comprise a fragment of SEQ ID NO:10 or a complement thereof and function as DNA primers when used together in an amplification reaction with DNA comprising cotton event Gh_CSM63718 to produce an amplicon diagnostic for cotton event Gh_CSM63718 in a sample. For example, the first and second DNA molecules can comprise SEQ ID NO:19 and SEQ ID NO:20. The amplicon described herein may comprise a DNA sequence comprising one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, or a fragment of any of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10 wherein the fragment is at least 10 nucleotides in length and comprises nucleotides 1,000–1,001 or 17,736–17,737 of SEQ ID NO:10. According to present embodiments, the sequence of an amplicon comprises at least one junction sequence or two junction sequences, such as a 5’ junction sequence and / or a 3’ junction sequences for cotton event Gh_CSM63718. Amplification and detection of such an amplicon is indictive or diagnostic for cotton event Gh_CSM63718.

[0165] For practical purposes, one should design primers which produce amplicons of a limited size range, for example, between 100 to 1000 bases. Smaller (shorter polynucleotide length) sized amplicons in general are more reliably produced in thermal amplification reactions, allow for shorter cycle times, and can be easily separated and visualized on agarose gels or adapted for use in endpoint TaqMan®-like assays. Smaller amplicons can be produced and detected by 61 US_ACTIVE\130153301\V-1methods known in the art of DNA amplicon detection. In addition, amplicons produced using the primer pairs can be cloned into vectors, propagated, isolated, and sequenced or can be sequenced directly with methods well established in the art. Any primer pair of forward and reverse primers, which may be identical or complementary to part of SEQ ID NO:10, such as SEQ ID NO:19 and SEQ ID NO:20, that is useful in a DNA amplification method to produce an amplicon diagnostic for cotton event Gh_CSM63718 or progeny thereof is an aspect of the disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:10, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for cotton event Gh_CSM63718 or progeny thereof is an aspect of the disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:12, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for plants comprising cotton event Gh_CSM63718 or progeny thereof is an aspect of the disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:9, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for cotton event Gh_CSM63718 or progeny thereof is an aspect of the disclosure.

[0166] A primer is typically designed to hybridize specifically to a complementary target DNA strand to form a hybrid between the primer and the target DNA strand. Hybridization or binding of a primer to the complementary target DNA strand is a point of recognition by a polymerase to begin extension of the primer (i.e., polymerization of additional nucleotides into a lengthening nucleotide molecule) using the target DNA strand as a template. Primer pairs refer to use of two primers binding opposite strands of a double stranded nucleotide segment for the purpose of amplifying the polynucleotide segment between the positions targeted for binding by the individual members of the primer pair, typically in a thermal amplification reaction or other conventional nucleic-acid amplification methods. Primer pairs are typically designed to hybridize to different nearby target positions of a template DNA molecule on opposing strands of the template DNA molecule such that the intervening region or sequence between the two primers can be specifically amplified for use or detection through multiple rounds of amplification.

[0167] To detect the presence or absence of cotton event Gh_CSM63718, the target positions and / or the intervening region or sequence of a template DNA molecule may comprise at least one 62 US_ACTIVE\130153301\V-1junction sequence and / or at least a portion of the insert of cotton event Gh_CSM63718. To detect the absence of cotton event Gh_CSM63718, the target positions and / or the intervening region or sequence of a template DNA molecule may comprise cotton genomic DNA that does not include a junction sequence or any portion of the insert of cotton event Gh_CSM63718. Thus, the presence or absence of an amplicon with a primer pair may be diagnostic of the presence or absence, respectively, of cotton event Gh_CSM63718 in a DNA molecule or sample, or vice versa. This may also be possible with more than one primer pair. For example, a first primer pair may produce a first amplicon if cotton event Gh_CSM63718 is present, and a second primer pair may produce a second amplicon if cotton event Gh_CSM63718 is absent or not present. Alternatively, the size of an amplicon produced in an amplification reaction may also be diagnostic of the presence or absence of cotton event Gh_CSM63718 in a DNA molecule or sample – e.g., a primer pair may produce a first amplicon of a first size if cotton event Gh_CSM63718 is present or a second amplicon of a second size if cotton event Gh_CSM63718 is absent and not present; or a first primer pair may produce a first amplicon of a first size if cotton event Gh_CSM63718 is present, and a second primer pair may produce a second amplicon of a second size if cotton event Gh_CSM63718 is absent or not present. According to some of these embodiments, at least two primer pairs may be used wherein at least one of the primer pairs is used as an internal control and is not associated with cotton event Gh_CSM63718.

[0168] According to present embodiments, a primer pair to detect the presence or absence of all or part of cotton event Gh_CSM63718 in a DNA molecule or sample comprises a first primer and a second primer, wherein the first primer is complementary to a 5’ flanking genomic DNA sequence and the second primer is complementary to a sequence within the transgenic insert; or wherein the first primer is complementary to a 5’ flanking genomic DNA sequence and the second primer is complementary to a 3’ flanking genomic DNA sequence; or wherein the first primer is complementary to a sequence within the transgenic insert and the second primer is complementary to a 3’ flanking genomic DNA sequence. Each reference in this paragraph to a primer complementary to a 5’ flanking genomic DNA sequence, a 3’ flanking genomic DNA sequence, or a sequence within the transgenic insert of cotton event Gh_CSM63718 is also intended to potentially include a primer complementary to the reverse complement or opposing strand of the 63 US_ACTIVE\130153301\V-1respective 5’ flanking genomic DNA sequence, 3’ flanking genomic DNA sequence, or sequence within the transgenic insert of cotton event Gh_CSM63718.

[0169] Illustrative DNA molecules useful as primers for detection of cotton event Gh_CSM63718 are provided as SEQ ID NO:19 and SEQ ID NO:20. The primer pair SEQ ID NO:19 and SEQ ID NO:20 can be useful as a first primer (complementary to a sequence within the 3’ flanking genomic DNA sequence) and a second primer (corresponding to a sequence within the transgenic insert), wherein each primer has sufficient length of consecutive nucleotides of SEQ ID NO:10 or a sequence complementary to SEQ ID NO:10 to function as DNA primers that, when used together in an amplification reaction with template DNA derived from cotton event Gh_CSM63718, hybridize to opposite strands of the template DNA and produce an amplicon diagnostic for cotton event Gh_CSM63718 DNA in a sample. The primer pair SEQ ID NO:19 (complementary to a 3’ flanking genomic DNA sequence) and SEQ ID NO:22 (corresponding to a 5’ flanking genomic DNA sequence) are useful as a first primer and a second primer, wherein each primer has sufficient length of consecutive nucleotides of a locus within the cotton genome to function as DNA primers that, when used together in a thermal amplification reaction with template DNA, to produce an amplicon indictive or diagnostic for the wildtype DNA for the zygosity of Gh_CSM63718 event DNA in a sample. An amplicon diagnostic for event Gh_CSM63718 comprises a sequence not naturally found in the cotton genome.

[0170] A primer may further comprise an oligo tail sequence such as those used in the Kompetitive Allele-Specific PCR (KASPTM) method. The allele-specific primers each harbor a unique tail sequence that corresponds with a universal FRET (fluorescence resonant energy transfer) cassette; one labelled with FAM™ dye and the other with HEX™ dye. During thermal cycling, the relevant allele-specific primer binds to the template and elongates, thus attaching the tail sequence to the newly synthesized strand. The complement of the allele-specific tail sequence is then generated during subsequent rounds of PCR, enabling the FRET cassette to bind to the DNA. The FRET cassette is no longer quenched and emits fluorescence.

[0171] Methods for designing and using primers and probes are well known in the art. DNA molecules comprising fragments of SEQ ID NOs:1–10 are useful as primers and probes for detecting cotton event Gh_CSM63718 and can readily be designed by one of skill in the art using the sequences provided herein. Such probes and primers are selected to be of sufficient length and 64 US_ACTIVE\130153301\V-1sequence complementarity to a target sequence to hybridize specifically to a target sequence under stringency hybridization conditions. Probes and primers may have a complete sequence complementarity or identity with the target sequence, although probes and primers differing from the target sequence in terms of identity or complementarity but retaining the ability to form a stable double-stranded structure under particular hybridization conditions or reaction conditions and to hybridize to the target sequence may be designed by conventional methods.

[0172] Any conventional nucleic acid hybridization or amplification method can be used to identify or detect the presence of a target DNA from a transgenic plant, such as cotton event Gh_CSM63718, in a sample. Polynucleotide molecules or DNA molecules, also referred to as “polynucleotide segment or fragment of sufficient length” or “sufficient length of contiguous or consecutive nucleotides” therefore are capable of specifically hybridizing to a target DNA sequence under certain hybridization conditions or reaction conditions. As used herein, the term “of sufficient length” refers to any length that is sufficient to be useful in a detection method of choice. Probes and primers are generally at least about 8 nucleotides, at least about 10 nucleotides, at least about 12 nucleotides, at least about 14 nucleotides, at least about 16 nucleotides, at least about 18 nucleotides, at least about 20 nucleotides, at least about 22 nucleotides, at least about 24 nucleotides, at least about 26 nucleotides, at least about 28 nucleotides, or at least about 30 nucleotides or more in length. Such probes and primers hybridize specifically to a target DNA sequence under stringent hybridization conditions.

[0173] As used herein, two nucleic acid molecules are capable of specifically hybridizing to one another if the two molecules are capable of forming an anti-parallel, double-stranded nucleic acid structure. A nucleic acid molecule is the “complement” of another nucleic acid molecule if they exhibit complete complementarity. As used herein, two nucleic acid molecules exhibit “complete complementarity” and are “completely complementary” if every nucleotide of the first nucleic acid molecule is complementary to every nucleotide of the second nucleic acid molecule when they are aligned. Two molecules are “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “low stringency” conditions. Similarly, the molecules are “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “high stringency” conditions. Conventional stringency conditions 65 US_ACTIVE\130153301\V-1are described by Haymes et al., In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985), and by MR Green and J Sambrook, Molecular cloning: a laboratory manual, 4thEdition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2012). Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure. In order for a nucleic acid molecule to serve as a primer or probe, it need only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations and other conditions employed.

[0174] As used herein, a substantially homologous or complementary sequence in relation to a reference nucleic acid sequence is a nucleic acid sequence that will specifically hybridize to the reference nucleic acid sequence or its complement to which it is being compared under high stringency conditions. As used herein, “stringent hybridization conditions” refers to conditions under which a polynucleotide will hybridize to its target sequence, typically in a complex mixture of nucleic acids, but to essentially no other sequences. “Stringent conditions” or “stringent hybridization conditions” when referring to a polynucleotide probe, refer to conditions under which a probe will hybridize to its target sequence to a detectably greater degree than to other sequences (e.g., at least 2-fold over background). Stringent conditions are sequence- dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures. Generally, stringent conditions are selected to be about 5-10° C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The Tmis the temperature (under defined ionic strength, pH, and nucleic acid concentration) at which 50% of the probes complementary to the target hybridize to the target sequence at equilibrium (as the target sequences are present in excess, at Tm, 50% of the probes are occupied at equilibrium). Stringent conditions will be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01 to 1.0 M sodium ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C for short probes (e.g., 10 to 50 nucleotides) and at least about 60°C for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. By controlling the stringency of the hybridization and / or washing conditions, target sequences that are 100% complementary to the probe can be identified (homologous probing). Alternatively, 66 US_ACTIVE\130153301\V-1stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of identity are detected (heterologous probing).

[0175] Appropriate stringency conditions which promote DNA hybridization, for example, 6× sodium chloride / sodium citrate (SSC) at about 45°C, followed by a wash of 2×SSC at 50°C, are known to those skilled in the art or can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0 x SSC at 50°C to a high stringency of about 0.2 x SSC at 50°C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, about 22°C, to high stringency conditions at about 65°C. Both temperature and salt may be varied, or either the temperature or the salt concentration may be held constant while the other variable is changed. Regarding the amplification of a target polynucleotide (e.g., by PCR) using a particular amplification primer pair, “stringent conditions” or “stringent hybridization conditions” are conditions that permit the primer pair to hybridize to the target polynucleotide to which a primer having the corresponding sequence (or its complement) would bind and to produce an identifiable amplification product (the amplicon) having a cotton Gh_CSM63718 event specific region in a DNA thermal amplification reaction. The term “specific for” a target sequence indicates that a probe or primer hybridizes under stringent hybridization conditions only to the target sequence in a sample comprising the target sequence.

[0176] A polynucleotide molecule or DNA molecule of the present disclosure, such as a primer or a probe, will specifically hybridize to at least one of the nucleic acid molecule sequences selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to SEQ ID NO:10, or a complete complement of or fragment of any of the foregoing under stringent hybridization conditions, or under moderately stringent hybridization conditions if the sequence of the polynucleotide molecule is not identical to the at least one of the nucleic acid molecules. The hybridization of a nucleic acid molecule, such as a primer or probe, to the target DNA molecule can be detected by any number of methods known 67 US_ACTIVE\130153301\V-1to those skilled in the art, which can include, but are not limited to, fluorescent tags, radioactive tags, antibody-based tags, and chemiluminescent tags.

[0177] An illustrative DNA molecule or polynucleotide useful as a probe for detecting cotton event Gh_CSM63718 is provided as SEQ ID NO:21. In some embodiments, a DNA molecule that functions as a probe comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, a complement of any of the foregoing or a fragment of any of the foregoing. In other embodiments, a DNA molecule comprises a polynucleotide segment of sufficient length to function as a DNA probe specific for at least one of: a) a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718; b) a 3’ junction sequence between the transgenic insert of cotton event Gh_CSM63718 and flanking cotton genomic DNA; c) SEQ ID NO:9; or d) a fragment of SEQ ID NO:9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO:9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718 in a sample of DNA.

[0178] A diagnostic amplicon produced by the methods described herein may be detected by a plurality of techniques known in the art, such as sequencing, restriction mapping, Southern analysis, or any other suitable polynucleotide or DNA hybridization, blotting, polymerization and / or amplification-based approach or technique. One method is Genetic Bit Analysis (Nikiforov et al., 1994) where a DNA oligonucleotide is designed that overlaps both the adjacent flanking genomic DNA sequence and the inserted DNA sequence – i.e., a junction sequence. The oligonucleotide is immobilized in wells of a microtiter plate. Following PCR of the region of interest (using, for example, one primer in the inserted sequence and one in the adjacent flanking genomic sequence), a single-stranded PCR product can be hybridized to the immobilized oligonucleotide and serve as a template for a single base extension reaction using a DNA polymerase and labeled dideoxynucleotide triphosphates (ddNTPs) specific for the expected next base. Readout may be fluorescent or ELISA-based. A signal indicates presence of the transgene / genomic junction sequence due to successful amplification, hybridization, and single base extension.

[0179] Another method is the pyrosequencing technique as described by Winge (2000). In this method, an oligonucleotide is designed that overlaps the adjacent genomic DNA and insert DNA 68 US_ACTIVE\130153301\V-1junction. The oligonucleotide is hybridized to single-stranded PCR product from the region of interest (one primer in the inserted sequence and one in the flanking genomic sequence) and incubated in the presence of a DNA polymerase, ATP, sulfurylase, luciferase, apyrase, adenosine 5’ phosphosulfate and luciferin. DNTPs are added individually and the incorporation results in a light signal that is measured. A light signal indicates the presence of the transgene / genomic sequence due to successful amplification, hybridization, and single or multi-base extension.

[0180] Fluorescence Polarization as described by Chen et al. (1999) is a method that can be used to detect the amplicon of the present invention. Using this method an oligonucleotide is designed that overlaps the genomic flanking and inserted DNA junction. The oligonucleotide is hybridized to single-stranded PCR product from the region of interest (one primer in the inserted DNA and one in the flanking genomic DNA sequence) and incubated in the presence of a DNA polymerase and a fluorescent-labeled ddNTP. Single base extension results in incorporation of the ddNTP. Incorporation can be measured as a change in polarization using a fluorometer. A change in polarization indicates the presence of the transgene / genomic sequence due to successful amplification, hybridization, and single base extension.

[0181] Real-time polymerase chain reaction (PCR) has the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data are collected throughout the PCR process, rather than at the end of the PCR. In real-time PCR, reactions are characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. In a real-time PCR assay, a positive reaction is detected by accumulation of a fluorescent signal. The higher the starting copy number of the nucleic acid target, the sooner a significant increase in fluorescence is observed. The cycle threshold (Ct value) is defined as the number of cycles required for the fluorescent signal to cross the threshold (i.e., exceeds background level). Ct levels are inversely proportional to the amount of target nucleic acid in the sample (i.e., the lower the Ct value, the greater the amount of target nucleic acid in the sample).

[0182] Taqman® (PE Applied Biosystems, Foster City, CA) is a method of detecting and quantifying the presence of a DNA sequence using real-time PCR and is fully described in the instructions provided by the manufacturer. Briefly, a FRET oligonucleotide probe is designed that overlaps the genomic flanking and insert DNA junction. The FRET probe and PCR primers (one 69 US_ACTIVE\130153301\V-1primer in the insert DNA sequence and one in the flanking genomic sequence) are cycled in the presence of a thermal stable polymerase and dNTPs. Hybridization of the FRET probe results in cleavage and release of the fluorescent moiety away from the quenching moiety on the FRET probe. A fluorescent signal indicates the presence of the transgene / genomic sequence due to successful amplification and hybridization.

[0183] Molecular beacons have been described for use in sequence detection as described in Tyangi et al. (1996). Briefly, a FRET oligonucleotide probe is designed that overlaps the flanking genomic and insert DNA junction. The unique structure of the FRET probe results in it containing secondary structure that keeps the fluorescent and quenching moieties in close proximity. The FRET probe and PCR primers (one primer in the insert DNA sequence and one in the flanking genomic sequence) are cycled in the presence of a thermostable polymerase and dNTPs. Following successful PCR amplification, hybridization of the FRET probe to the target sequence results in the removal of the probe secondary structure and spatial separation of the fluorescent and quenching moieties. A fluorescent signal results and indicates the presence of the flanking / transgene insert sequence due to successful amplification and hybridization.

[0184] Other detection methods known in the art may be used. For example, microfluidics (see, e.g., U.S. Patent Publication No. 2006 / 068398; U.S. Patent No. 6,544,734) provide methods and devices that can be used to separate and amplify DNA samples or molecules. Optical dyes can be used to detect and measure specific DNA molecules (see, e.g., WO / 05017181). Nanotube devices (see, e.g., WO / 06024023) that comprise an electronic sensor for the detection of DNA molecules or nanobeads that bind specific DNA molecules can then be detected. Nanopore sequencing technology, such as that described in Wang et al. (2021), Tayler et al. (2018), or Pearson et al. (2019), can also be used for event detection.

[0185] The DNA molecules and corresponding nucleotide sequences provided herein are therefore useful for, among other things, identifying cotton event Gh_CSM63718, detecting the presence of DNA derived from the transgenic cotton event Gh_CSM63718 in a sample, and monitoring samples for the presence and / or absence of cotton event Gh_CSM63718 or plant parts derived from cotton plants comprising event Gh_CSM63718.

[0186] Provided are proteins that can be used to produce antibodies for detecting the presence of cotton event Gh_CSM63718 in a sample. Such antibodies are specific for the PAT, DMO, TDO, 70 US_ACTIVE\130153301\V-1EPSPS or PPO proteins that are encoded by cotton event Gh_CSM63718. Methods for preparing a polyclonal antibody or a monoclonal antibody are well known to those skilled in the art and can be used to make antibodies specific for the PAT, DMO, TDO, EPSPS or PPO proteins encoded by cotton event Gh_CSM63718. For example, U.S. Patent No. 9,371,394 describes antibodies to the PAT enzyme; U.S. Patent No. 7,838,729 and Wang et al. (2016) describe antibodies to DMO; USDA APHIS Petition 04_08601p describes antibody to CP4 EPSPS; Lermontova et al (1997) describes antibodies to a PPO protein. The DNA sequence encoding the PAT, DMO, TDO, EPSPS and PPO proteins are provided in SEQ ID NO:10 and the start positions and stop positions of the coding sequences are indicated in Table 1. The DNA sequences encoding the proteins and the proteins encoded by the sequences are useful to produce antibodies for detecting the presence of cotton event Gh_CSM63718 by the methods described herein. Detection for the presence of cotton event Gh_CSM63718 may be done by using any protein detection techniques known in the art, such as Western blot analysis, immuno-precipitation, enzyme-linked immunosorbent assay (ELISA), antibody attachment to a detectable label or reporter molecule (such as a radioactive isotope, ligand, chemiluminescent agent, or enzyme), or enzymatic action on a reporter molecule. One method provides for contacting a sample with an antibody or antibodies that bind to at least one of the PAT, DMO, TDO, EPSPS and PPO proteins encoded by cotton event Gh_CSM63718 and then detecting the presence or absence of antibody binding. The binding of such antibody is diagnostic for the presence of one or more proteins encoded by cotton event Gh_CSM63718.

[0187] An alternative to antibody for protein detection is the aptamer-based detection method for detecting proteins or molecules of interest in a sample. As used herein, the term “aptamer(s)” or “aptamer sequences(s)” refers to short synthetic single-stranded oligonucleotide molecules with high-affinity and specificity binding to a target molecule such as a protein, polypeptide, lipid, glycoprotein, glycolipid, glycopeptide, saccharide, or polysaccharide by forming distinct tertiary structures (Ellington and Szostak, 1990; Robertson and Joyce, 1990; Tuerk and Gold, 1990; Wang et al., 2019). The single-stranded nucleic acid can be ssDNA, RNA or derivatives of either thereof. The aptamer comprises a three-dimensional structure held in certain conformation(s) that provide intermolecular contacts to specifically bind its given target. Although aptamers are nucleic acid- based molecules, the binding to the target molecule is not entirely dependent on a linear base sequence, but rather a particular secondary / tertiary / quaternary structure. The term aptamer also 71 US_ACTIVE\130153301\V-1covers next generation aptamers such as X aptamers that cannot typically be amplified by PCR but can be adapted by adding a link primer. Such aptamers can specifically bind to proteins of interest but can also be easily amplified, sequenced etc. in a downstream process. The term aptamer also covers aptamers that include modified bases. It is envisaged that the aptamers may include traditional aptamers of 15 to 120 bases in length, as well as longer aptamers of approximately 200 bases in length (e.g., Ultramers® by Integrated DNA Technologies, Inc. Coralville, Iowa, USA). To detect the protein of interest in a sample, aptamers specific to the protein of interest are obtained and are then incubated with the sample. If the protein of interest is present in the sample, protein- aptamer conjugates are formed. Methods for detecting the aptamer / protein complex are known in the art, such as aptablotting or South-Western blot (Li et al., 2017; Sekhon et al., 2017), aptamer- based Western blot (Wang at al., 2020), aptamer sandwich assay (Svobodova et al., 2021). Chemical modifications, additional functional groups, and / or linkers can be added to the nucleic acid aptamer to provide increased binding affinity to a target protein, and to provide a convenient means to detect the target molecule. Such tags and or labels can comprise fluorescent, luminescent, absorbance, or radioactive-based chemical groups, or can comprise an enzyme or substrate that provides a detectable response such as a precipitate, either alone or in the presence of other factors.

[0188] Nucleic acid or protein detection kits for detecting the presence of cotton event Gh_CSM63718 are provided. Variations on such kits can also be developed using the compositions and methods disclosed herein and the methods well known in the art for protein and nucleic acid detection for identification of cotton event Gh_CSM63718. Protein and nucleic acid detection kits can be applied to methods for breeding with plants comprising cotton event Gh_CSM63718. Such kits contain primers and / or probes or antibodies or aptamers which are specific to cotton event Gh_CSM63718. Such DNA primers and / or probes may comprise fragments of one or more of SEQ ID NOs:1-10, or antibodies specific for a protein encoded by the cotton event Gh_CSM63718. The kits can also contain instructions for using the primers, probes, antibodies, or aptamers for detecting the presence of cotton event Gh_CSM63718. Kits may optionally also comprise reagents for performing the detection or diagnostic reactions described herein.

[0189] One example of a detection kit comprises at least one DNA molecule of sufficient length of contiguous nucleotides of SEQ ID NO:10 to function as a DNA probe useful for detecting 72 US_ACTIVE\130153301\V-1the presence or absence of cotton event Gh_CSM63718 in a sample. The DNA derived from transgenic cotton plants comprising event Gh_CSM63718 would comprise a DNA molecule having at least one sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, a complement of any of the foregoing, or a fragment of any of the foregoing. An illustrative DNA molecule sufficient for use as a probe is one comprising the sequence provided as SEQ ID NO:21. Other probes may be readily designed by one of skill in the art. The probe can include a junction sequence that spans the 5’ or 3’ junction between the cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718.

[0190] Another example of a detection kit comprises at least one primer pair that specifically hybridize to a target DNA and amplify a diagnostic amplicon under the appropriate reaction conditions useful for detecting the presence or absence of cotton event Gh_CSM63718 in a sample. A kit that contains DNA primers that are homologous or complementary to any portion of the cotton genomic region as set forth in SEQ ID NO:11 or 12 and to any portion of the inserted transgenic DNA as set forth in SEQ ID NO:9 is an object of the present disclosure. The kit may provide an agarose gel-based detection method or any number of methods of detecting the amplicon that are known in the art. Such a method may also include sequencing the amplicon or a fragment thereof. Illustrative DNA molecules sufficient for use as a primer pair are ones comprising the sequences provided as SEQ ID NO:19 and SEQ ID NO:20, and SEQ ID NO:19 and SEQ ID NO:22, respectively, wherein the primer pair SEQ ID NO:19 and SEQ ID NO:20 will produce an amplicon diagnostic for the presence of event Gh_CSM63718 in a sample; and the primer pair SEQ ID NO:19 and SEQ ID NO:22 will produce an amplicon indictive of wild-type DNA, therefore, diagnostic for the absence of event Gh_CSM63718 in a sample. Other primer pairs may be readily designed by one of skill in the art.

[0191] Another example of a detection kit comprises at least one antibody specific for the PAT, TDO, DMO, EPSPS and / or PPO proteins encoded by cotton event Gh_CSM63718. For example, such a kit may utilize a lateral flow strip comprising reagents activated when the tip of the strip is contacted with an aqueous solution. Illustrative proteins for use in antibody production are the PAT, TDO, DMO, EPSPS, and PPO proteins encoded by the sequence provided as SEQ ID NO:10, or any fragment thereof. For example, an antibody specific for the TDO protein encoded 73 US_ACTIVE\130153301\V-1by cotton event Gh_CSM63718 and / or an antibody specific for the PPO protein encoded by cotton event Gh_CSM63718 can be used. Detection of binding of the antibody to the one or more proteins encoded by cotton event Gh_CSM63718 in a sample is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.

[0192] The detection kits provided herein are useful for, among other things, identifying cotton event Gh_CSM63718, selecting plant varieties or hybrids comprising cotton event Gh_CSM63718, detecting the presence of DNA derived from the transgenic cotton plant comprising event Gh_CSM63718 in a sample, and monitoring samples for the presence and / or absence of cotton plants comprising event Gh_CSM63718, or plant parts derived from cotton plants comprising event Gh_CSM63718.

[0193] Cotton plants, progeny, seeds, cells, and plant parts comprising cotton event Gh_CSM63718 are provided, as well as commodity products produced using these. As used herein, the term “cotton” or “cotton” means plant species within Gossypium hirsutum, Gossypium barbadense, Gossypium arboretum, Gossypium herbaceum and all plant varieties belonging to the genus Gossypium that can be bred with Gossypium hirsutum plants. The term “cotton” is intended to include cotton plants, plant parts, plant cells, plant tissue, seeds, progeny plants, and / or cotton commodity products. These cotton plants, plant parts, plant cells, plant tissues, seeds, progeny plants and commodity products contain or comprise cotton event Gh_CSM63718 or are derived from a transgenic cotton plant, plant part, plant cell, plant tissue, seed, progeny plant or commodity product containing or comprising event Gh_CSM63718. These cotton plants, plant parts, plant cells, plant tissues, seeds, progeny plants and commodity products contain a detectable amount of a polynucleotide or DNA molecule comprising at least one junction sequence and / or heterologous transgenic insert sequence of cotton event Gh_CSM63718, such as a polynucleotide or nucleic acid or DNA molecule having or comprising at least one of the sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, a polynucleotide comprising at least 16 consecutive nucleotides of SEQ ID NO:1, at least 16 consecutive nucleotides of SEQ ID NO:2, at least 33 consecutive nucleotides of SEQ ID NO:3, at least 32 consecutive nucleotides of SEQ ID NO:4, at least 53 consecutive nucleotides of SEQ ID NO:5, or at least 52 consecutive nucleotides of SEQ ID NO:6, a polynucleotide comprising a sequence that is at least 90%, at least 91%, at least 92%, 74 US_ACTIVE\130153301\V-1at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9, and a complete complement of any of the foregoing. In some embodiments, the cotton plant, plant part, plant cell, plant tissue, or seed is further defined as a progeny plant of any generation of a cotton plant comprising cotton event Gh_CSM63718, or a cotton plant part, plant seed, or plant cell derived therefrom.

[0194] As used herein, “cotton event Gh_CSM63718” or “cotton event Gh_CSM63718 locus” refers to the genomic locus of the cotton event Gh_CSM63718 or a modified cotton event Gh_CSM63718 comprising the complete or partial flanking, junction and insertion sequences of the cotton event Gh_CSM63718 or the modified cotton event Gh_CSM63718. A modified cotton event Gh_CSM63718 comprises one or more mutations, edits and / or genetic modifications such as deletion, insertion, inversion or transposition in the cotton event Gh_CSM63718 locus relative to the cotton event Gh_CSM63718.

[0195] A modified cotton event Gh_CSM63718 and methods of making a modified cotton event Gh_CSM63718 are provided. As is described further herein, various mutagenesis or targeted genome editing techniques and related tools exist or could be made or engineered to permit genetic modification or mutation of the transgenic insert, junction and / or the flanking genomic DNA of cotton event Gh_CSM63718, such as by deletion, insertion, transposition, inversion, and / or substitution of nucleic acid sequence(s), or by insertion or introduction of a guide RNA target site or a cognate guide RNA recognition target (CgRRS), and the transgenic event as modified may still be uniquely characterized by the presence of heterologous DNA and / or one or more sequences of the insertion, junction(s) and / or flanking sequence described herein at the particular position in the genome previously occupied by the unmodified cotton event Gh_CSM63718 relative to flanking portions or sequences of the native cotton genome. According to present embodiments, a modified transgenic event derived from cotton event Gh_CSM63718 may comprise all or part of the insertion sequence and / or transgene cassette of cotton event Gh_CSM63718 and / or one or more complete or partial flanking sequences described herein. As used herein, a “modified cotton event Gh_CSM63718” refers to the genomic DNA or sequence of the cotton event Gh_CSM63718 locus comprising one or more 75 US_ACTIVE\130153301\V-1mutations, edits, or genetic modifications relative to the genomic DNA or sequence of the cotton event Gh_CSM63718, wherein such mutations or edits or genetic modifications are introduced or made by a mutagenesis or targeted genome editing technique of a cotton plant, plant part, tissue or cell comprising the cotton event Gh_CSM63718. A “modified cotton event Gh_CSM63718” includes a “further modified cotton event Gh_CSM63718” made by first inserting a target site or cognate target site or CgRRS into the cotton event Gh_CSM63718 locus and then further modifying the cotton event Gh_CSM63718 locus as described herein. Methods and techniques of mutagenesis are known in the art and include, for example, chemical mutagenesis (i.e., treatment with a chemical mutagen, such as an azide, hydroxylamine, nitrous acid, acridine, nucleotide base analog, or alkylating agent - e.g., EMS (ethylmethane sulfonate), MNU (N-methyl-N-nitrosourea), etc.), physical mutagenesis (e.g., gamma rays, X-rays, UV, ion beam, other forms of radiation, etc.), insertional mutagenesis (e.g., transposon or T-DNA insertion), and site directed mutagenesis through genome editing. Cotton plants, plant parts, plant seeds, plant tissues, and plant cells described herein include cotton plants, parts, seeds, tissues and cells comprising a modified cotton event Gh_CSM63718 or a further modified cotton event Gh_CSM63718.

[0196] The cotton plants, plant parts, plant cells, plant tissues, seeds, progeny plants and commodity products express or comprise at least one herbicide tolerance gene selected from the group consisting of PAT, TDO, DMO, EPSPS, PPO, and any combination thereof, and are tolerant to at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor such as mesotrione, dicamba, glyphosate, a PPO herbicide, and any combination thereof. Illustrative PPO herbicides include, for example, but are not limited to flumioxazin, epyrifenacil, lactofen, acifluorfen, pyraflufen, pyraflufen-ethyl, oxadiazon, butafenacil, carfentrazone-ethyl, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyclopropylmethyl (2-{2-chloro- 76 US_ACTIVE\130153301\V-14-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoximacil), fomesafen, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazin, 1-ethoxy-1- oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, and 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro- 5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, 77 US_ACTIVE\130153301\V-1methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, ethyl 1-({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate, and combinations of any thereof.

[0197] The present disclosure provides cotton plants, progeny, seeds, plant cells, and plant parts such as microspores, pollen, anthers, ovules, ovaries, flowers, bolls, embryos, stems, buds, nodes, leaves, roots, and calli derived from a transgenic cotton plant comprising cotton event Gh_CSM63718. A representative sample of seed comprising cotton event Gh_CSM63718 has been deposited according to the Budapest Treaty for the purpose of enabling the present disclosure. The ATCC repository has assigned Accession No. PTA-127638 to the seed comprising cotton event Gh_CSM63718.

[0198] A microorganism is provided. The microorganism comprises a polynucleotide molecule having the nucleotide sequence of SEQ ID NO:9, or a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:9. An example of such a microorganism is an Agrobacterium cell. Another example of such a microorganism is an E. coli cell.

[0199] A plant cell is provided comprising a polynucleotide molecule as described herein. For example, a plant cell is provided having a nucleotide sequence present in its genome, wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and a nucleic acid molecule comprising a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9.

[0200] Plant cells and microorganisms of the present disclosure are useful in many industrial applications, including but not limited to: (i) use as research tools for scientific inquiry or industrial 78 US_ACTIVE\130153301\V-1research; (ii) use in culture for producing endogenous or recombinant carbohydrate, lipid, nucleic acid, enzymes or protein products or small molecules that may be used for subsequent scientific research or as industrial products; and (iii) for the plant cells of the present disclosure, use with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that may then be used for agricultural research or production. The production and use of such transgenic plant cells utilize modern microbiological techniques and human intervention to produce a man- made, unique plant cell. In this process, a recombinant DNA is inserted into a plant cell’s genome to create a transgenic plant cell that is separate and unique from naturally occurring plant cells. This transgenic plant cell can then be cultured much like bacteria and yeast cells using modern microbiology techniques and may exist in an undifferentiated, unicellular state. The new plant cell’s genetic composition and phenotype is a technical effect created by the integration of a heterologous DNA into the genome of the cell.

[0201] Provided are methods of using a plant cell, such as transgenic plant cells. These include (i) methods of producing transgenic cells by integrating a recombinant DNA into the genome of the cell and then using this cell to derive additional cells possessing the same heterologous DNA; (ii) methods of culturing cells that contain recombinant DNA using modern microbiology techniques; (iii) methods of producing and purifying endogenous or recombinant carbohydrate, lipid, nucleic acid, enzymes or protein products from cultured cells; and (iv) methods of using modern plant tissue culture techniques with transgenic plant cells to produce transgenic plants or transgenic plant tissue cultures.

[0202] The cotton plants, progeny, seeds, cells, and plant parts of the present disclosure may contain one or more additional desirable trait(s) or transgenic event(s). Such desirable traits may be transgenic traits, native traits, or mutant or edited traits or alleles produced by other methods such as genome editing, base editing, prime editing or other conventional mutagenesis methods. Desirable traits may be combined with cotton event Gh_CSM63718 by, for example, crossing a cotton plant comprising cotton event Gh_CSM63718 with another cotton plant containing the additional trait(s), or transgenic events. Such traits or transgenic events include, but are not limited to, increased insect resistance, increased water use efficiency, increased yield performance, increased drought resistance, increased disease resistance, increased seed or fiber quality, improved nutritional quality, hybrid seed production, and / or increase herbicide tolerance, in which 79 US_ACTIVE\130153301\V-1the trait is measured with respect to a cotton plant lacking such transgenic trait. Many cotton transgenic events are known to those of skill in the art. For example, a list of such traits is provided by the United States Department of Agriculture’s (USDA) Animal and Plant Health Inspection Service (APHIS) and can be found on their website www.aphis.usda.gov on the worldwide web. For example, any of the cotton plants, plant seeds, plant parts, or plant cells of the present disclosure can further comprise additional transgenes selected from the group consisting of Cry1B.3, Cry1Da_7, Vip3Cb1.1, Cry2Ab, and combinations of any thereof. An illustrative Cry1B.3 coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO:224 and SEQ ID NO:225, respectively. An illustrative Cry1Da_7 coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO:226 and SEQ ID NO:227, respectively. An illustrative Vip3Cb1.1 coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO:228 and SEQ ID NO:229, respectively. An illustrative Cry2Ab coding sequence and its corresponding amino acid sequence are provided as SEQ ID NO:230 and SEQ ID NO:231, respectively. For example, the cotton plant, plant seed, plant part, or plant cell can further comprise cotton event Gh_BCS246002 and / or cotton event MON15947. The genetic elements in the transgenic inserts of cotton event Gh_BCS246002 and cotton event MON15947 are described further hereinbelow. These additional transgenes can provide cotton plants resistance to infestations by Lepidopteran pests such as Cotton Bollworm (Helicoverpa zea), Tobacco Budworm (Heliothis virescens), Fall Armyworm (Spodoptera frugiperda), Old World Bollworm (Helicoverpa armigera), and any combination of any two or three or all four of the foregoing pest species.

[0203] Alternatively or in addition, the cotton Gh_CSM63718 event can be stacked by breeding or by retransformation or by site directed integration / introgression with other events or combinations of events known in the art including, but not limited to: • 19-51a (DD-01951A-7 for herbicide tolerance, described in USDA-APHIS Petition 95- 256-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • 281-24-236 (also known as DAS-24236-5 for herbicide tolerance and insect resistance, deposited as ATCC PTA-6233 and described in US Patent Nos. 7,179,965 and 7,883,850, 80 US_ACTIVE\130153301\V-1the entire contents and disclosure of which are incorporated herein by reference in their entirety), • 3006-210-23 (also known as DAS-21023-5 for herbicide tolerance and insect resistance, deposited as ATCC PTA-6233, and described in US Patent Nos.7,179,965 and 7,883,850, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • 31707, 31803, 31807, 31808 and 42317 (also known as BXN™ Plus Bollgard™ Cotton for herbicide tolerance and insect resistance, described in USDA-APHIS Petition 97-013- 01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • BXN10211 (also known as 10211, BXN-10211-9, BXN™ Cotton for herbicide tolerance, described in USDA-APHIS Petition 93-196-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • BXN10215 (also known as 10215, BXN-10215-4, BXN™ Cotton for herbicide tolerance, described in USDA-APHIS Petition 93-196-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • BXN10222 (also known as 10222, BXN-10222-2, BXN™ Cotton for herbicide tolerance, described in USDA-APHIS Petition 93-196-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • BXN10224 (also known as IR102, for herbicide tolerance, described in USDA-APHIS Petition 93-196-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • COT102 (also known as IR102, SYN-IR102-7 for insect resistance, described in US Patent No. 7,371,940, and in USDA-APHIS Petition 03-155-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • COT202 (for insect resistance, described in US Patent No. 7,521,550, the entire contents and disclosure of which are incorporated herein by reference in their entirety), 81 US_ACTIVE\130153301\V-1• COT203 (for insect resistance, described in PCT Patent Publication No. WO2005054480, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • COT67B (also known as IR67B, SYN-IR67B-1 for insect resistance, described in USDA- APHIS Petition 07-108-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • 1143-14A (for insect resistance, described in PCT Patent Publication No. WO2006128570, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • 1143-51B (for insect resistance, described in PCT Patent Publication No. WO2006128569, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • CE43-67B (for insect resistance, described in US Patent Nos.7,834,254 and 9,131,651, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • CE44-69D (for insect resistance, described in US Patent Publication No.20100024077, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • CE46-02A (for insect resistance, described in PCT Patent Publication No. WO2006128572, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • DAS81910 (also known as DAS-81910-7 for herbicide tolerance, deposited as ATCC PTA-12456, and described in US Patent Nos. 9,551,024 and 9,896,718, and in USDA- APHIS Petition 13-262-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • GHB119 (also known as BCS-GH005-8, EE-GH6 for insect resistance, deposited as ATCC PTA-8398, and described in US Patent Nos. 8,309,818 and 9,328390, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), 82 US_ACTIVE\130153301\V-1• GHB614 (also known as BCS-GH002-5, EE-GH3, and GlyTol™ for herbicide tolerance, deposited as ATCC PTA-6878, and described in US Patent Nos.7,932,439, 8,501,411 and 9,394,566, and in USDA-APHIS Petition 06-332-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • GHB811 (also known as BCS-GH811-4 for herbicide tolerance, described in USDA- APHIS Petition 17-138-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • LLCotton25 (also known as ACS-GH001-3, EE-GH1, and Fibermax™ Liberty Link™ for herbicide tolerance, deposited as ATCC PTA-3343, and described in US Patent Nos. 6,818,807, 7,442,504 and 7,834,168, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • T303-3 (also known as BCS-GH003-6 for herbicide tolerance and insect resistance, described in USDA-APHIS Petition 12-033-01p (Extension of 08-340-01p), the entire contents and disclosure of which are incorporated herein by reference in their entirety), • T304-40 (also known as BCS-GH004-7 and EE-GH5 for herbicide tolerance and insect resistance, deposited as ATCC PTA-8171, and described in US Patent Nos. 8,247,654, 9,382,550 and 10,356,996, and in USDA-APHIS Petition 08-340-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • T342-142 (for insect resistance, described in PCT Patent Publication No WO2006128568, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON1076 (also known as 1076, MON-89924-2 and Bollgard™ Cotton for insect resistance, described in USDA-APHIS Petition 94-308-0lp, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON1445 (also known as 1445, MON-01445-2, and Roundup Ready™ Cotton for herbicide tolerance, described in US Patent Nos. 6,740,488, 7,189,514, 7,807,357 and 7,820,392, and in USDA-APHIS Petition 95-045-0lp, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), 83 US_ACTIVE\130153301\V-1• MON15985 (also known as MON-15985-7, and Bollgard II™ Cotton for insect resistance, deposited as ATCC PTA-2516, described in US Patent Nos. 7,223,907, 7,858,764 and 9,133,473, and in USDA-APHIS Petition 00-342-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • MON15947 (for insect resistance, described in USDA-APHIS Confirmation Request 21- 211-01cr and subsequent USDA confirmation, as well as US Patent Nos. 7,223,907, 7,858,764 and 9,133,473, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON1698 (also known as MON-89383-1, and Roundup Ready™ Cotton for herbicide tolerance, described in USDA-APHIS Petition 95-045-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON531 (also known as MON-00531-6, and Bollgard™ Cotton, Ingard™ for insect tolerance, described in USDA-APHIS Petition 94-308-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON757 (also known as MON-00757-7, and Bollgard™ Cotton for insect tolerance, described in USDA-APHIS Petition 94-308-01p, the entire contents and disclosure of which are incorporated herein by reference in their entirety), • MON88701 (also known as MON-88701-3, and Dicamba-Glufosinate Tolerant Cotton for herbicide tolerance, deposited as ATCC PTA-11754, described in US Patent Nos. 8,735,661, 9,024,115, 10,030,277 and 10,774,341, and in USDA-APHIS Petition 12-185- 01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • MON88702 (also known as MON-88702-4 for insect resistance, deposited as ATCC PTA- 122520, described in US Patent Nos. 10,604,769 and 11,286,499, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • MON88913 (also known as MON-88913-8, and Roundup Ready™ Flex™ Cotton for herbicide tolerance, deposited as ATCC PTA-4854, described in US Patent Nos.7,381,861, 8,071,735 and 8,435,743, and in USDA-APHIS Petition 04-086-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), 84 US_ACTIVE\130153301\V-1• TAM66274 (also known as TAM-66274-5 for modified product quality, deposited as ATCC PTA-124218, described in US Patent No. 10,604,764, and in USDA-APHIS Petition 17-292-01p, the entire contents and disclosure of each of which are incorporated herein by reference in their entirety), • 281-24-236 x 3006-210-23 (also known as MXB-13, DAS-24236-5 x DAS-21023-5, and WideStrike™ Cotton for insect resistance), • 281-24-236 x 3006-210-23 x COT102 (also known as DAS-24236-5 x DAS-21023-5 x SYN-IR102-7 for insect resistance), • 281-24-236 x 3006-210-23 x COT102 x 81910 (also known as DAS-24236-5 x DAS- 21023-5 x SYN-IR102-7 x DAS-81910-7 for insect resistance and herbicide tolerance), • 3006-210-23 x 281-24-236 x MON1445 (also known as DAS-21023-5 x DAS-24236-5 x MON-01445-2, and WideStrike™ Roundup Ready™ Cotton for insect resistance and herbicide tolerance), • 3006-210-23 x 281-24-236 x MON88913 (also known as DAS-21023-5 x DAS-24236-5 x MON-88913-8, and Widestrike™ Roundup Ready Flex™ Cotton for insect resistance and herbicide tolerance), • 3006-210-23 x 281-24-236 x MON88913 x COT102 (also known as DAS-21023-5 x DAS- 24236-5 x MON-88913-8 x SYN-IR102-7, and Widestrike™ x Roundup Ready Flex™ x VIPCOT™ Cotton for insect resistance and herbicide tolerance), • 3006-210-23 x 281-24-236 x MON88913 x COT102 x DAS81910 (also known as DAS- 21023-5 x DAS-24236-5 x MON-88913-8 x SYN-IR102-7 x DAS-81910-7 for insect resistance and herbicide tolerance), • 31807 x 31808 (for insect resistance and herbicide tolerance), • COT102 x COT67B (also known as SYN-IR102-7 x SYN-IR67B-1 and VIPCOT™ Cotton for insect resistance), 85 US_ACTIVE\130153301\V-1• COT102 x COT67B x MON88913 (also known as SYN-IR102-7 x SYN-IR67B-1 x MON- 88913-8 and VIPCOT™ Roundup Ready Flex™ Cotton for insect resistance and herbicide tolerance), • COT102 x MON15985 (also known as SYN-IR102-7 x MON-15985-7 and Bollgard® III for insect resistance), • COT102 x MON15985 x MON88913 (also known as SYN-IR102-7 x MON-15985-7 x MON-88913-8 and Bollgard® III x Roundup Ready™ Flex™ for insect resistance and herbicide tolerance), • COT102 x MON15985 x MON88913 x MON88701 (also known as SYN-IR102-7 x MON-15985-7 x MON-88913-8 x MON88701-3 for insect resistance and herbicide tolerance), • GHB614 x LLCotton25 (also known as BCS-GH002-5 x ACS-GH001-3 and GlyTol™ Liberty Link™ for herbicide tolerance), • GHB614 x LLCotton25 x MON15985 (also known as BCS-GH002-5 x ACS-GH001-3 x MON-15985-7 for insect resistance and herbicide tolerance), • GHB614 x MON15985 (also known as BCS-GH002-5 x MON-15985-7 for insect resistance and herbicide tolerance), • GHB614 x T304-40 x GHB119 (also known as BCS-GH002-5 x BCS-GH004-7 x BCS- GH005-8 and Glytol™ x Twinlink™ for insect resistance and herbicide tolerance), • GHB614 x T304-40 x GHB119 x COT102 (also known as BCS-GH002-5 x BCS-GH004- 7 x BCS-GH005-8 x SYN-IR102-7 and GlyTol LibertyLink TwinLink® Plus for insect resistance and herbicide tolerance), • GHB811 x T304-40 x GHB119 x COT102 (also known as BCS-GHB811-4 x BCS- GH004-7 x BCS-GH005-8 x SYN-IR102-7 for insect resistance and herbicide tolerance), • LLCotton25 x MON15985 (also known as ACS-GH001-3 x MON-15985-7 and Fibermax™ Liberty Link™ Bollgard II™ for insect resistance and herbicide tolerance), 86 US_ACTIVE\130153301\V-1• MON15985 x MON1445 (also known as MON-15985-7 x MON-01445-2 and Roundup Ready™ Bollgard II™ Cotton for insect resistance and herbicide tolerance), • MON531 x MON1445 (also known as MON-00531-6 x MON-01445-2 and Roundup Ready™ Bollgard™ Cotton for insect resistance and herbicide tolerance), • MON88701 x MON88913 (also known as MON 88701-3 x MON-88913-8 for herbicide tolerance), • MON88701 x MON88913 x MON15985 (also known as MON 88701-3 x MON-88913-8 x MON-15985-7 for insect resistance and herbicide tolerance), • MON88913 x MON15985 (also known as MON-88913-8 x MON-15985-7 and Roundup Ready™ Flex™ Bollgard II™ Cotton for insect resistance and herbicide tolerance), • T304-40 x GHB119 (also known as BCS-GH004-7 x BCS-GH005-8 and TwinLink™ Cotton for insect resistance and herbicide tolerance, described in in USDA-APHIS Petition 08-340-01p, the entire content and disclosure of which are incorporated herein by reference in their entirety), • T304-40 x GHB119 x COT102 (also known as BCS-GH004-7 x BCS-GH005-8 x SYN- IR102-7 for insect resistance and herbicide tolerance).

[0204] As used herein, “Gh_BCS246002” refers to cotton event Gh_BCS246002. Cotton seed comprising event Gh_BCS246002 has been deposited under ATCC Accession No. 127733 and is fully described and characterized in US Patent Application No.63 / 645,146, the entire contents and disclosure of which are incorporated herein by reference in their entirety. Transgenic cotton plants comprising cotton event Gh_BCS246002 comprise SEQ ID NO:212 (5’ cotton genomic flank sequence + transgenic insert + 3’ cotton genomic flank sequence), SEQ ID NO:213 (transgenic insert), SEQ ID NOs:214-217 (5’ junction sequences), and SEQ ID NOs:218-221 (3’ junction sequences). The transgenic insert in cotton plants comprising event Gh_BCS246002 comprises three expression cassettes as shown in Table 2. In the first expression cassette, the DNA sequence encoding the Cry1B.3 protein in cotton event Gh_BCS246002 is operably linked to Cucumis melo Chlorophyl a / b binding protein promoter and leader (see, e.g., United States Patent 10,550,401, 87 US_ACTIVE\130153301\V-1referenced therein as SEQ ID NO:29) and a Medicago truncatula hypothetical protein 3´ UTR (or transcription termination sequence, see, e.g., United States Patent 10,501,749, referenced therein as SEQ ID NO:3). In the second expression cassette, the DNA sequence encoding the Cry1Da_7 protein in cotton event Gh_BCS246002 is operably linked to a synthetic promoter, leader, and intron (see, e.g., United States Patent Application Publication 2018 / 0216129 A1, referenced therein as SEQ ID NOs:5, 6, and 9) and a Medicago truncatula expansin-related protein 1 precursor protein 3´ UTR (or transcription termination sequence, see, e.g., United States Patent Application Publication 2014 / 0283200 A1, referenced therein as SEQ ID NO:7). In the third expression cassette, the DNA sequence encoding the Vip3Cb1.1 protein in cotton event Gh_BCS246002 is operably linked to an Arabidopsis thaliana Actin-2 promoter, leader, and intron, and a Medicago truncatula seed maturation protein PM21 3´ UTR (or transcription termination sequence, see, e.g., United States Patent Application Publication 2014 / 0283200 A1, referenced therein as SEQ ID NO:16). Expression (transcription into the mRNAs coding for the toxin amino acid sequences, and translation of the mRNAs into the toxin proteins) of the toxin proteins Cry1B.3, Cry1Da_7, and Vip3Cb1.1 from their respective transgene cassettes is oriented in the same direction (head to tail / head to tail / head to tail). The open reading frames are provided in the event in the following order in the 5’ to 3’ direction: the ORF for Cry1B.3, the ORF for Cry1Da_7, and the ORF for Vip3Cb1.1, and as specified above, these three ORFs are comprised within SEQ ID NO:212 and SEQ ID NO:213.

[0205] Expression of the Cry1B.3, Cry1Da_7, and Vip3Cb1.1 insect inhibitory proteins in cotton event Gh_BCS246002 provides resistance to the larval forms of Lepidopteran insect pests including, but not limited to, Cotton Bollworm (Helicoverpa zea), Tobacco Budworm (Heliothis virescens), and Fall Armyworm (Spodoptera frugiperda). Table 2. Transgenic cassettes and elements in cotton event Gh_BCS246002 Element Description88 US_ACTIVE\130153301\V-1Element Description h ´ l i f hl h ll m to e

[0206] As used herein, “MON15947” refers to cotton event MON15947, which corresponds to event MON15985 without MON531 for insect resistance, an event developed by ballistic methods into germplasm containing event MON531, and which was determined to be unlinked to MON531. Cotton seed comprising event MON15985 has been deposited under ATCC Accession No. PTA-2516 and is fully described and characterized in US Patent Nos. 7,223,907, 7,858,764 89 US_ACTIVE\130153301\V-1and 9,133,473, the entire contents and disclosure of which are incorporated herein by reference in their entirety. Transgenic cotton plants comprising cotton event MON15947 comprise SEQ ID NO:232 (a 2,267-nucleotide sequence representing 1,877 nucleotides of the 5’ flank region of the cotton genomic DNA and 390 nucleotides of the integrated transgene insert), and SEQ ID NO:233 (a 1,360-nucleotide sequence representing 349 nucleotides of the integrated transgene insert and 1,012 nucleotides of the 3’ flank region of the cotton genomic DNA). The transgenic insert in cotton plants comprising event MON15947 comprises a Cry2Ab expression cassette, comprising the cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region (US Patents 5,530,196, 5,424,200, and 5,359,142) operably connected or linked to a petunia heat shock protein untranslated leader sequence (PetHSP70-leader) operably-connected or linked to the N-terminal chloroplast transit peptide from Arabidopsis thaliana EPSPS gene (AEPSPS / CTP2) (Van den Broeck et. al.1985) operably connected or linked to a synthetic sequence encoding Cry2Ab protein (Widner and Whiteley, 1990) which is operably connected or linked to the 3′ nontranslated region of the nopaline synthase (NOS) gene from Agrobacterium tumifaciens which terminates transcription and directs polyadenylation (Fraley et al. 1983). Cotton plants comprising event MON15985 confers resistance to Lepidopteran insect pests.

[0207] Any of the cotton plants, plant parts, seeds, cells, progeny or commodity products described herein that comprise cotton event Gh_CSM63718 can further comprise cotton event Gh_BCS46002 and / or MON15947.

[0208] Any of the cotton plants, plant parts, seeds, cells, progeny or commodity products described herein can further comprise a recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:212; SEQ ID NO:213; SEQ ID NO:214; SEQ ID NO:215 SEQ ID NO:216; SEQ ID NO:217; SEQ ID NO:218; SEQ ID NO:219; SEQ ID NO:220; SEQ ID NO:221; SEQ ID NO:222; SEQ ID NO:223; SEQ ID NO:232; SEQ ID NO:233; a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:212 or the full length of SEQ ID NO:213, and a complete complement of any of the foregoing. 90 US_ACTIVE\130153301\V-1

[0209] Plants comprising cotton event Gh_CSM63718 and cotton event Gh_BCS246002 and / or cotton event MON15947 can be made by any method known in the art. For example, Plants comprising cotton event Gh_CSM63718 and / or cotton event Gh_BCS246002 and / or cotton event MON15947 can be made by crossing a cotton plant comprising cotton event Gh_CSM63718 with a cotton plant comprising cotton event Gh_BCS246002 or cotton event MON15947 and selecting for progeny plants containing both Gh_CSM63718 and Gh_BCS246002 or selecting for progeny plants containing both Gh_CSM63718 and MON15947. Plants comprising cotton events Gh_CSM63718, Gh_BCS246002 and MON15947 can be made, for example, by crossing a cotton plant comprising event Gh_CSM63718 with a cotton plant comprising both Gh_BCS246002 and MON15947, or by crossing a cotton plant comprising event Gh_BCS246002 with a cotton plant comprising event MON15947 first, selecting for a progeny plant comprising both Gh_BCS246002 and MON15947; and then crossing the progeny plant comprising both Gh_BCS246002 and MON15947 with a plant comprising event Gh_CSM63718, and selecting for progeny plants comprising Gh_BCS246002, MON15947 and Gh_CSM63718. Alternatively, a cotton plant comprising cotton event Gh_CSM63718 can be crossed with a cotton plant comprising event Gh_BCS246002 (or event MON15947) first, selecting for a progeny plant comprising both cotton events Gh_CSM63718 and Gh_BCS246002 (or MON15947), and then crossing the progeny plant comprising both Gh_CSM63718 and Gh_BCS246002 (or MON15947) with a plant comprising event MON15947 (or Gh_BCS246002), and selecting for a progeny plant comprising all three events.

[0210] The term “site-specific nuclease” refers to any enzyme that can cleave a nucleotide sequence in a site-specific manner. Site-specific nucleases allow for the precise and / or targeted editing of a specific location in a genome of a plant. Site-specific nucleases include, for example, RNA guided nucleases, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs).

[0211] Some site-specific nucleases, such as zinc finger nucleases (ZFNs) and TALENs, are not RNA-guided and instead rely on their protein structure to determine their target site for causing a DSB (double stranded break) or nick, or they are fused, tethered or attached to a DNA-binding protein domain or motif. The protein structure of the site-specific nuclease (or the fused / attached / tethered DNA binding domain) targets the site-specific nuclease to the target site. 91 US_ACTIVE\130153301\V-1ZFNs, and TALENs, may be designed, engineered, and constructed according to known methods to target and bind to a target site.

[0212] RNA-guided nucleases are nucleases that form a complex (e.g., a ribonucleoprotein) with a guide RNA, which then guides the complex to a target site within a target sequence. One non-limiting example of guided nucleases are CRISPR nucleases. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) nucleases are proteins found in bacteria that are guided by guide RNAs (“gRNAs”) to a target nucleic acid molecule, where the endonuclease can then cleave one or two strands the target nucleic acid molecule. Although the origins of CRISPR nucleases are bacterial, many CRISPR nucleases have been shown to function in eukaryotic cells. CRISPR editing systems comprising a CRISPR associated protein (nuclease) and cognate guide RNAs (that can be transcribed from guide DNA polynucleotides) may be used for targeted DNA cleavage or modification. The CRISPR-associated protein can be selected from a Type I CRISPR-associated protein, a Type II CRISPR-associated protein, a Type III CRISPR-associated protein, a Type IV CRISPR-associated protein, a Type V CRISPR-associated protein, or a Type VI CRISPR- associated protein, such as, but not limited to, Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Cas12a (also known as Cpf1), Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, CasX, CasY, and Mad7.

[0213] As used herein, the term “target site,” “genomic target site,” “target genomic nucleic acid,” or “target cotton genomic nucleic acid,” refers to a polynucleotide sequence that is sufficiently unique in the cotton genome to allow targeted genome modification by a site-specific nuclease. In one aspect, the sequence of the target site is changed from the wildtype sequence, namely the target site is edited. In another aspect, the target site is the site of insertion of a DNA sequence of interest.

[0214] The target site can comprise one or more of the criteria selected from the group consisting of: (i) the target site selected is more than 2 kb from a gene (ii) the target site selected is more than 1000 nucleotides (nt) from a small RNA hotspot, and (iii) the target site comprises 700 nucleotides of unique sequence on either side, Target site selection criteria are further described in US Patent Application Publication No. 2020 / 0024610 and US Provisional Patent 92 US_ACTIVE\130153301\V-1Application No. 63 / 387,871, the entire contents and disclosure of which are incorporated herein by reference in their entirety.

[0215] The target site comprises a sequence that is recognized by a site-specific nuclease. In some embodiments, the target site comprises a sequence that is recognized by a site-specific nuclease resulting in precise or targeted cleavage within the target site. For example, the site- specific nuclease can be selected from the group consisting of an RNA-guided nuclease, a Zinc Finger nuclease, and a TALEN. In some embodiments, the target site comprises a PAM (Protospacer Adjacent Motif) sequence that is recognized by an RNA-guided nuclease (e.g., a CRISPR nuclease system). For example, the target site can comprise a PAM motif that is recognized by a Cas12a / Cpf1 CRISPR nuclease system. The target site can further comprise a sequence that is recognized by and hybridizes to a CRISPR guide RNA. In some embodiments, the target site comprises a sequence that is recognized by and hybridizes to a Cas12a / Cpf1 CRISPR guide RNA.

[0216] A DNA sequence of interest can be inserted at a target site using a site-specific nuclease. As used herein, the term “DNA sequence of interest” or “donor sequence” or “donor DNA” refers to a nucleic acid / DNA sequence that has been selected for targeted insertion into a cotton genomic sequence. In one aspect, the cotton genomic sequence is a genomic target site described above. A DNA sequence on interest can be of any length, for example between 2 and 50,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 1,000 and 5,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 5,000 and 10,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 10,000 and 15,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 15,000 and 20,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 20,000 and 25,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 25,000 and 30,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 30,000 and 35,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 35,000 and 40,000 93 US_ACTIVE\130153301\V-1nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 40,000 and 45,000 nucleotides in length (or any integer value therebetween). In some embodiments, the DNA sequence is between about 45,000 and 50,000 nucleotides in length (or any integer value therebetween). A DNA sequence may comprise one or more gene expression cassettes that further comprise actively transcribed and / or translated gene sequences. For example, the DNA sequence of interest can comprise a gene expression cassette comprising a sequence selected from: an herbicide tolerance gene, an insecticidal resistance gene, a nitrogen use efficiency gene, a water use efficiency gene, a nutritional quality gene, a DNA binding gene, a selectable marker gene, a target site for a site-specific nuclease, and any combination thereof. Alternatively, the DNA sequence of interest may comprise a polynucleotide sequence which does not comprise a functional gene expression cassette or an entire gene (e.g., may comprise regulatory sequences such as promoters, enhancers, etc.), or may not contain any identifiable gene expression elements or any actively transcribed gene sequence. In some embodiments, the DNA of interest may comprise a cognate guide RNA recognition site (CgRRS). In some embodiments, the DNA of interest will have at least one homology arm DNA sequence. The term “homology arm DNA sequence” refers to a polynucleotide sequence that has at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to a target sequence in a plant or plant cell. Further, the DNA sequence can be linear or circular, and can be single- stranded or double-stranded. It can be delivered to the cell as naked nucleic acid, as a complex with one or more delivery agents (e.g., liposomes, poloxamers, T-strand encapsulated with proteins, etc.) or contained in a bacterial or viral delivery vehicle, such as, for example, Agrobacterium tumefaciens or a Gemini Virus, or a nanovirus, respectively.

[0217] Once a specific target site is identified, a site-specific nuclease targeting the selected target site can be designed and introduced into the plant, seed, or plant cell. For example, a CRISPR-associated nuclease (e.g., Cas12a / Cpf1) and at least one RNA guide molecule that can hybridize to the target site can be designed and cloned into a plant expression vector and delivered to the plant, seed, or plant cell. If the genome modification is designed to induce a double-strand break (DSB) (i.e., induce a cleavage) with non-homologous end joining (NHEJ) repair for introduction of insertions and deletions (indels), then just the engineered CRISPR nuclease and at 94 US_ACTIVE\130153301\V-1least one RNA guide molecule is delivered to the plant, seed or cell. If a DNA sequence of interest is to be incorporated at the target site, then the engineered CRISPR nuclease, at least one RNA guide molecule, and the DNA of interest are co-delivered to the plant, seed, or cell. The DNA of interest may integrate into the target site by NHEJ (Non-homologous End Joining) or by homology-dependent repair (HR). In the latter case, the DNA of interest will have at least one homology arm DNA sequence. An alternative to delivery of the engineered CRISPR nuclease as a DNA expression construct is the delivery of a ribonucleoprotein (RNP) complex of the CRISPR associated nuclease protein in complex with the guide RNA.

[0218] Following delivery of the site-specific nuclease to a plant cell, the cells or plants regenerated from the cells are sampled to confirm the presence of the intended site-specific genome modification including insertion of the DNA sequence of interest at or proximal to the target site. Methods of detecting the genome modification are known to one skilled in the art, and include PCR, TaqMan® PCR, droplet digital PCR (ddPCR™, Bio-Rad Laboratories, Hercules, Calif.), sequencing, Sanger sequencing, ABI 3730 DNA fragment analysis (Applied Biosystems, Grand Island, N.Y.), Southern blot analysis, Northern blot analysis, phenotypic analysis, or any other technique known to one in the art to detect genome modification.

[0219] The plants described herein can be used to produce progeny or offspring that comprise cotton event Gh_CSM63718. Such progeny may include any plant, seed, and cell and / or regenerable plant part comprising cotton event Gh_CSM63718 inherited or derived from an ancestor or parental cotton plant(s), at least one of which comprises a DNA molecule having or comprising at least one polynucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, a polynucleotide comprising at least 23 consecutive nucleotides of SEQ ID NO:1, at least 16 consecutive nucleotides of SEQ ID NO:2, at least 33 consecutive nucleotides of SEQ ID NO:3, at least 32 consecutive nucleotides of SEQ ID NO:4, at least 53 consecutive nucleotides of SEQ ID NO:5, or at least 52 consecutive nucleotides of SEQ ID NO:6, or a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, 95 US_ACTIVE\130153301\V-1at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9.

[0220] Cotton plants, progeny, and seeds may be homozygous or heterozygous for the event Gh_CSM63718 and the transgenes of event Gh_CSM63718. Progeny may be grown from seeds produced by a cotton plant comprising or containing event Gh_CSM63718 and / or from seeds produced by a plant fertilized with pollen from a cotton plant comprising or containing event Gh_CSM63718 (i.e., fertilized with pollen comprising or containing event Gh_CSM63718). Plants or progeny may also be obtained by tissue culture and regeneration methods from a protoplast, cell, embryo or reproductive or somatic tissue derived from a cotton plant comprising or containing cotton event Gh_CSM63718.

[0221] Progeny plants may be self-pollinated (also known as “selfing”) to generate a true breeding line of plants, i.e., plants homozygous for the cotton event Gh_CSM63718 DNA. Alternatively, progeny plants may be outcrossed, i.e., bred with another plant, to produce a varietal or a hybrid seed or plant. The other plant may be transgenic or non-transgenic. A varietal or hybrid seed or plant of the present disclosure may thus be derived by crossing a first parent that lacks the specific and unique DNA of event Gh_CSM63718 with a second parent comprising event Gh_CSM63718, resulting in a hybrid comprising the specific and unique DNA of event Gh_CSM63718. Each parent can be a hybrid or an inbred / variety, so long as the cross or breeding results in a plant or seed of the present disclosure, i.e., a seed having at least one allele comprising the specific and unique DNA of event Gh_CSM63718 and / or at least 16 consecutive nucleotides of SEQ ID NO:1, at least 16 consecutive nucleotides of SEQ ID NO:2, at least 33 consecutive nucleotides of SEQ ID NO:3, at least 32 consecutive nucleotides of SEQ ID NO:4, at least 53 consecutive nucleotides of SEQ ID NO:5, or at least 52 consecutive nucleotides of SEQ ID NO:6, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9.

[0222] Sexually crossing one plant with another plant, i.e., cross-pollinating, may be accomplished or facilitated by human intervention, for example: by human hands collecting the 96 US_ACTIVE\130153301\V-1pollen of one plant and contacting this pollen with the style or stigma of a second plant; by human hands and / or human actions removing, destroying, or covering the stamen or anthers of a plant (e.g., by manual intervention or by application of a chemical gametocide) so that natural self- pollination is prevented and cross-pollination would have to take place in order for fertilization to occur; by human placement of pollinating insects in a position for “directed pollination” (e.g., by placing beehives in orchards or fields or by caging plants with pollinating insects); by human opening or removing of parts of the flower to allow for placement or contact of foreign pollen on the style or stigma; by selective placement of plants (e.g., intentionally planting plants in pollinating proximity); and / or by application of chemicals to precipitate flowering or to foster receptivity (of the stigma for pollen).

[0223] Two different transgenic plants of the same or different genetic backgrounds may thus be crossed to produce inbred or hybrid offspring plants, plant parts and / or seeds that contain two independently segregating transgenes or events wherein at least one of those transgenes or events comprises or is contained within cotton event Gh_CSM63718. For example, transgenic plants comprising cotton event Gh_CSM63718 can be crossed with other transgenic cotton plants to produce a plant having the characteristics of both transgenic parents.

[0224] Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other breeding methods that are commonly used for different traits and crops are known in the art and can be found in one of several references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987).

[0225] A plant part is provided. As used herein, a “plant part” refers to any part of a plant that is comprised of material directly from or derived from a plant comprising cotton event Gh_CSM63718. Plant parts include but are not limited to microspores, pollen, anthers, ovules, ovaries, flowers, bolls, embryos, buds, nodes, stems, leaves, roots, and calli, in whole or part. Plant parts may be viable or nonviable. Plant parts may be regenerable or non-regenerable.

[0226] Nonliving or nonregenerable cotton plant materials are provided herein. The nonliving or nonregenerable cotton plant material can comprise any of the recombinant DNA molecules characteristic of cotton event Gh_CSM63718 described herein, or any of the DNA constructs described herein. The nonliving or nonregenerable cotton plant material can comprise cotton event 97 US_ACTIVE\130153301\V-1Gh_CSM63718, a representative sample of seed comprising the cotton event cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638.

[0227] Commodity products that comprise any of the DNA molecules characteristic of cotton event Gh_CSM63718 or any of the DNA constructs described herein are provided. Such commodity products can be produced from plants comprising cotton event Gh_CSM63718. The commodity products contain a detectable amount of DNA comprising a DNA sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9. As used herein, a “commodity product” refers to any composition or product which is comprised of material from plant, seed, cell, or plant part comprising cotton event Gh_CSM63718. Commodity products may be viable or non-living plant material, that is, a material that is not living and derived from a plant, seed, cell, or plant part comprising cotton event Gh_CSM63718. Nonviable commodity products include but are not limited to nonviable seeds, whole or processed seeds, processed plant tissues or plant parts, dehydrated plant tissues or parts, frozen plant tissues or parts, food for human consumption such as cottonseed oil; plant parts processed for animal feed such as cottonseed cake, cottonseed meal and cottonseed hulls; cotton fiber and linters; plant parts processed into fertilizer, biochar and building boards such as cotton stalks. Viable commodity products include but are not limited to viable seeds, viable plant parts (such as pollen, ovule, ovary, flower, boll, embryo, node, bud, root and leaf) and viable plant cells. A plant comprising event Gh_CSM63718 can thus be used to manufacture any commodity product typically acquired from a cotton plant. Any such commodity product that is derived from the plants comprising event Gh_CSM63718 may contain at least a detectable amount of the specific and unique DNA corresponding to event Gh_CSM63718, and specifically may contain a detectable amount of a polynucleotide having a nucleotide sequence of at least 22 consecutive nucleotides of SEQ ID NO:1, at least 16 consecutive nucleotides of SEQ ID NO:2, at least 33 consecutive nucleotides of SEQ ID NO:3, at least 32 consecutive nucleotides of SEQ ID NO:4, at least 53 consecutive 98 US_ACTIVE\130153301\V-1nucleotides of SEQ ID NO:5, or at least 52 consecutive nucleotides of SEQ ID NO:6, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9. Any standard method of detection for polynucleotide molecules may be used, including methods of detection disclosed herein.

[0228] Methods for producing such commodity products are also provided. Such methods comprise: (a) obtaining a transgenic cotton plant, plant part, or plant seed comprising cotton event Gh_CSM63718; and (b) producing a commodity product from the transgenic cotton plant, plant part, or plant seed.

[0229] A plant tolerant to herbicides may be produced by sexually crossing a plant comprising event Gh_CSM63718 with another plant and thereby producing seed, which is then grown into progeny plants. For example, provided herein is a method of producing a progeny cotton plant comprising event Gh_CSM63718, the method comprising: (a) sexually crossing a first cotton plant that comprises cotton event Gh_CSM63718 with itself or a second cotton plant; (b) collecting one or more seeds produced from the cross; (c) growing one or more seeds to produce one or more progeny plants; and (d) selecting at least a first progeny plant or seed comprising cotton event Gh_CSM63718. Inbred and hybrid cotton plants comprising cotton event Gh_CSM63718 produced by such methods are also provided.

[0230] The progeny plants may be analyzed using diagnostic methods to select for progeny plants that comprise event Gh_CSM63718 DNA or for progeny plants tolerant to at least one herbicide selected from the group consisting of glufosinate, ß-triketone HPPD inhibitors, dicamba, glyphosate, PPO herbicides, and any combination thereof. Examples of ß-triketone HPPD inhibitors include mesotrione, benzobicyclon (BBC), tembotrione, sulcotrione, tefuryltrione, and any combination thereof. Examples of PPO herbicides include flumioxazin, epyrifenacil, lactofen, acifluorfen, pyraflufen, pyraflufen-ethyl, oxadiazon, butafenacil, carfentrazone-ethyl, pyridin-2- ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- 99 US_ACTIVE\130153301\V-1yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyclopropylmethyl (2-{2-chloro- 4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoximacil), fomesafen, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazin, 1-ethoxy-1- oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, and 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro- 5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 100 US_ACTIVE\130153301\V-12,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, ethyl 1-({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate, and combinations of any thereof. The other plant used may or may not be transgenic. The progeny plant and / or seed produced may be varietal or hybrid seed.

[0231] A plant tolerant to glufosinate, ß-triketone HPPD inhibitor herbicides, dicamba, glyphosate and PPO herbicides may be produced by selfing a plant comprising event Gh_CSM63718 comprising a polynucleotide having the nucleotide sequence of SEQ ID NOs:1- 10, at least 23 consecutive nucleotides of SEQ ID NO:1, at least 16 consecutive nucleotides of SEQ ID NO:2, at least 33 consecutive nucleotides of SEQ ID NO:3, at least 32 consecutive nucleotides of SEQ ID NO:4, at least 53 consecutive nucleotides of SEQ ID NO:5, or at least 52 consecutive nucleotides of SEQ ID NO:6, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9, or a complete complement of any of the foregoing, and thereby producing seed, which is then grown into progeny plants. These progeny plants may then be analyzed using diagnostic methods to select for progeny plants that comprise event Gh_CSM63718 DNA, or for progeny plants tolerant to the glufosinate, ß-triketone HPPD inhibitor herbicides, dicamba, glyphosate and PPO herbicides such as flumioxazin, epyrifenacil, lactofen, acifluorfen, pyraflufen, pyraflufen-ethyl, oxadiazon, butafenacil, carfentrazone-ethyl, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- 101 US_ACTIVE\130153301\V-1yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyclopropylmethyl (2-{2-chloro- 4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoximacil), fomesafen, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazin, 1-ethoxy-1- oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, and 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro- 5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 102 US_ACTIVE\130153301\V-12,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, ethyl 1-({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate, and combinations of any thereof.

[0232] Cotton event Gh_CSM63718 contains the PAT, TDO, DMO, EPSPS and PPO expression cassettes that provides tolerance to glufosinate, ß-triketone HPPD inhibitor herbicides (such as mesotrione, benzobicyclon (BBC), tembotrione, sulcotrione, tefuryltrione, and any combination thereof), dicamba, glyphosate, and PPO herbicides such as flumioxazin, epyrifenacil, lactofen, acifluorfen, pyraflufen, pyraflufen-ethyl, oxadiazon, butafenacil, carfentrazone-ethyl, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, cyclopropylmethyl (2-{2-chloro- 4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, methyl (2R)-2-{[(E)-({2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}methylidene)amino]oxy}propanoate (flufenoximacil), fomesafen, saflufenacil, sulfentrazone, tiafenacil, and trifludimoxazin, 1-ethoxy- 1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- 103 US_ACTIVE\130153301\V-1yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, and 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro- 5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene- 1,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, ethyl 1-({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate, and combinations of any thereof.

[0233] PPO herbicides include diphenylethers, N-phenylphthalimides, oxadiazoles, oxazolidinediones, phenylpyrazoles, pyrimidinediones, thiadiazoles, triazolinones, benzoxazinone derivatives, other PPO herbicides, and combinations of any thereof. Examples of diphenylethers include, but are not limited to, acifluorfen, bifenox, ethoxyfen, fluorodifen, fluoronitrofen, 104 US_ACTIVE\130153301\V-1furyloxyfen, halosafen, chlomethoxyfen, chlornitrofen, ethoxyfen-ethyl, fluoroglycofen, lactofen, nitrofen, oxyfluorfen, fomesafen, a salt of any thereof, and an ester of any thereof. Examples of N-phenylphthalimides include, but are not limited to, cinidon-ethyl, flumiclorac, flumiclorac- pentyl, and flumioxazin. Examples of oxadiazoles include, but are not limited to, oxadiargyl and oxadiazon. Examples of oxazolidinediones include, but are not limited to, pentoxazone. Examples of phenylpyrazoles include, but are not limited to, fluazolate, pyraflufen, and pyraflufen-ethyl. Examples of pyrimidinediones or phenyluracils include, but are not limited to, benzfendizone, butafenacil, epyrifencacil, flupropacil, flufenoximacil, saflufenacil, and tiafenacil. Examples of thiadiazoles include, but are not limited to, fluthiacet-methyl and thidiazimin. Examples of triazolinones include, but are not limited to, azafenidin, bencarbazone, carfentrazone, its salts and esters, and sulfentrazone. Examples of benzoxazinone derivatives include, but are not limited to, 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,4-dihydro-3-oxo-4-prop-2-ynyl-2H-1,4-benzoxazin-6- yl)-1,3,5-triazinane-2,4-dione (trifludimoxazin)). Examples of other PPO herbicides include, but are not limited to, chlorphthalim, flufenpyr, flufenpyr-ethyl, flumipropyn, pyraclonil, and profluazol. Further examples of other PPO herbicides include:

[0234] 1) an herbicidally active compound of the general formula (I) or an agrochemically acceptable salt thereof (I)in which: R1is hydrogen, R2is hydrogen, fluorine, chlorine, bromine, trifluoromethyl, methoxy, ethoxy, prop- 1-yloxy, or but-1-yloxy, 105 US_ACTIVE\130153301\V-1R3is hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2- yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1- yloxy, R4is fluorine, chlorine, bromine, cyano, NO2, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethynyl, propyn-1-yl, 1-butyn-1-yl, pentyn-1-yl, or hexyn-1-yl, R5, R6and R7are independently hydrogen, fluorine, chlorine, bromine, iodine, cyano, methyl, ethyl, prop-1-yl, 1-methylethyl, but-1-yl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1- dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1- dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,2-dimethylbutyl, 2,3- dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2- trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2- methylpropyl, trifluoromethyl, difluoromethyl, pentafluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, 1,1-dimethyleth-1-yloxy, difluoromethoxy, trifluoromethoxy, pentafluoroethoxy, 2,2-difluoroethoxy, or 2,2,2-trifluoroethoxy, G is methylene, (methyl)methylene, (ethyl)methylene, (prop-1-yl)methylene, (prop- 2-yl)methylene, (but-1-yl)methylene, (but-2-yl)methylene, (pent-1-yl)methylene, (pent-2-yl)methylene, (pent-3-yl)methylene, (dimethyl)methylene, (diethyl)methylene, ethylene, n-propylene, (1-methyl)ethyl-1-ene, (2- methyl)ethyl-1-ene, n-butylene, 1-methylpropyl-1-ene, 2-methylpropyl-1-ene, 3- methylpropyl-1-ene, 1,1-dimethylethyl-1-ene, 2,2-dimethylethyl-1-ene, 1- ethylethyl-1-ene, 2-ethylethyl-1-ene, 1-(prop-1-yl)ethyl-1-ene, 2-(prop-1-yl)ethyl- 1-ene, 1-(prop-2-yl)ethyl-1-ene, 2-(prop-2-yl)ethyl-1-ene, 1,1,2-trimethylethyl-1- ene, 1,2,2-trimethylethyl-1-ene, 1,1,2,2-tetramethylethyl-1-ene, n-pentylene, 1- methylbutyl-1-ene, 2-methylbutyl-1-ene, 3-methylbutyl-1-ene, 4-methylbutyl-1- ene, 1,1-dimethylpropyl-1-ene, 2,2-dimethylpropyl-1-ene, 3,3-dimethylpropyl-1- 106 US_ACTIVE\130153301\V-1ene, 1,2-dimethylpropyl-1-ene, 1,3-dimethylpropyl-1-ene, 1-ethylpropyl-1-ene, n- hexylene, 1-methylpentyl-1-ene, 2-methylpentyl-1-ene, 3-methylpentyl-1-ene, 4- methylpentyl-1-ene, 1,1-dimethylbutyl-1-ene, 1,2-dimethylbutyl-1-ene, 1,3-di- methylbutyl-1-ene, 2,2-dimethylbutyl-1-ene, 2,3-dimethylbutyl-1-ene, 3,3- dimethylbutyl-1-ene, 1-ethylbutyl-1-ene, 2-ethylbutyl-1-ene, 1,1,2- trimethylpropyl-1-ene, 1,2,2-trimethylpropyl-1-ene, 1-ethyl-1-methylpropyl-1- ene, or 1-ethyl-2-methylpropyl-1-ene, X and Y are independently O (oxygen) or S (sulfur) and Q is one of the following moieties Q-1 to Q-54, Q-56 to Q-57, Q-60 to Q-89, Q-91 to Q-129, Q-131 to Q-139, Q-141 to Q-144, Q-146 to Q-180, Q-182 to Q-185, Q-193 to Q-195, Q-200 to Q-208, Q-210 to Q-370, Q-395 to Q-440:107 US_ACTIVE\130153301\V-1108 US_ACTIVE\130153301\V-1Q-46 Q-47 Q-48 Q-49 Q-50109 US_ACTIVE\130153301\V-1110 US_ACTIVE\130153301\V-1111 US_ACTIVE\130153301\V-1112 US_ACTIVE\130153301\V-1Q-161 Q-162 Q-163 Q-164 Q-165113 US_ACTIVE\130153301\V-1Q-200114 US_ACTIVE\130153301\V-1115 US_ACTIVE\130153301\V-1Q-251 Q-252 Q-253 Q-254 Q-255NO N116 US_ACTIVE\130153301\V-1117 US_ACTIVE\130153301\V-1Q-306 Q-307 Q-308 Q-309 Q-310O118 US_ACTIVE\130153301\V-1O O N+119 US_ACTIVE\130153301\V-1120 US_ACTIVE\130153301\V-1121 US_ACTIVE\130153301\V-1

[0235] Examples of such herbicidally active compounds within the scope of formula (I) include:methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate;[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate; 122 US_ACTIVE\130153301\V-1N F O ethyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate; and FBrO N hyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate.

[0236] 2) an herbicidally active compound of the general formula (II) or an agrochemically acceptable salt thereofin which: W represents a group W-1 to W-3 123 US_ACTIVE\130153301\V-1R1represents hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1- yloxy, R2represents fluorine, chlorine, bromine, cyano, nitro, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethynyl, propyn-1-yl, 1-butyn-1-yl, pentyn-1-yl, or hexyn-1-yl, R3and R4independently of each other represent hydrogen, (C1-C8)-alkyl, R13O-(C1-C8)- alkyl, (C3-C8)-cycloalkyl, (C2-C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, or heterocyclyl-(C1-C8)-alkyl, or R3and R4together with the carbon atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered carbocyclic ring optionally having further substitution, R5represents hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, R13O-(C1-C8)-alkyl, (C2- C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3- C8)-cycloalkyl, aryl, heteroaryl, or heterocyclyl, R6represents hydrogen, fluorine, chlorine, bromine, trifluoromethyl, difluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, R7represents hydrogen or methyl, Q represents hydroxy or a group Q-1, Q-2 124 US_ACTIVE\130153301\V-1R8represents hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R13, C(O)OR13, or (C1-C8)-alkoxy- (C1-C8)-alkyl, R9represents hydrogen or (C1-C8)-alkyl, R10represents hydrogen, halogen, cyano, nitro, (C1C8)-alkyl, (C1C8)-haloalkyl, (C3C8)- cycloalkyl, (C3C8)-cycloalkyl-(C1C8)-alkyl, (C3C8)-halocycloalkyl, (C3C8)- halocycloalkyl-(C1C8)-alkyl, (C2C8)-alkenyl, (C2C8)-alkynyl, aryl, aryl-(C1C8)-alkyl, heteroaryl, heteroaryl-(C1C8)-alkyl, heterocyclyl, heterocyclyl-(C1C8)-alkyl, R11R12N- (C1C8)-alkyl, R13O-(C1C8)-alkyl, cyano-(C1C8)-alkyl, (C1C8)-alkylcarbonyloxy-(C1C8)- alkyl, (C3C8)-cycloalkylcarbonyloxy-(C1C8)-alkyl, arylcarbonyloxy-(C1C8)-alkyl, heteroarylcarbonyloxy-(C1C8)-alkyl, heterocyclylcarbonyloxy-(C1C8)-alkyl, OR13, NR11R12, SR14, S(O)R14, SO2R14, R14S-(C1C8)-alkyl, R14(O)S-(C1C8)-alkyl, R14O2S- (C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl-(C1C8)-alkyl, bis-[(C1C8)-alkyl](aryl)silyl(C1C8)- alkyl, [(C1C8)-alkyl]-bis-(aryl)silyl-(C1C8)-alkyl, tris-[(C1C8)-alkyl]silyl, bis- hydroxyboryl-(C1C8)-alkyl, bis-[(C1C8)-alkoxy]boryl-(C1C8)-alkyl, tetramethyl-1,3,2- Dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1C8)-alkyl, nitro-(C1C8)-alkyl, C(O)OR13, C(O)R13, C(O)NR11R12, R13O(O)C-(C1C8)-alkyl, R11R12N(O)C-(C1C8)-alkyl, or bis-(C1C8)-alkoxy-(C1C8)-alkyl, or R8and R10together with the carbon atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution, R11and R12independently of each other represent hydrogen, (C1-C8)-alkyl, (C2-C8)- alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)-cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, 125 US_ACTIVE\130153301\V-1(C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)- alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)- alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)-cycloalkenyl-(C1-C8)-alkyl, C(O)R13, SO2R14, heterocyclyl, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)- alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)- alkenyloxycarbonyl, (C2-C8)-alkynyloxycarbonyl, or heterocyclyl-(C1-C8)-alkyl, or R11and R12together with the nitrogen atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution, R13represents hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)- cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)- cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)- haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy- (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)- alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)- cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)- alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis- [(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)- alkyl-amino-(C2-C6)-alkyl, R14S-(C1-C8)-alkyl, R14(O)S-(C1-C8)-alkyl, R14O2S-(C1-C8)- alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris- [(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)- Alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)- cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy- (C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl, 126 US_ACTIVE\130153301\V-1R14represents hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)- cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)- cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl- (C1-C8)-alkyl, (C4-C10)-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)- alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3- C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azetidinyl, N- pyrrolidinyl, N-piperidinyl, or N-morpholinyl, and R15and R16independently of each other represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclyl.

[0237] Examples of such herbicidally active compounds within the scope of formula (II) include methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-({2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenyl}methylidene)amino]oxy}propanoate (also known as methyl (2R)-2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy} propanoate or flufenoximacil), methyl (2S)-2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy} propanoate, methyl 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5- [3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- 127 US_ACTIVE\130153301\V-1yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}-2- methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)- 3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy} butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} butanoate, 2- {[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl (2R)-2- ({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)- [2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- 128 US_ACTIVE\130153301\V-1fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, 2-{[(E)-{5- [3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, and (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid.

[0238] 3) an herbicidally active compound of the general formula (III) or an agrochemically acceptable salt thereofin which: W represents a group W-1 to W-3 O O O 7 R 7 R O129 US_ACTIVE\130153301\V-1R1represents hydrogen, fluorine, chlorine, bromine, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1- yloxy, R2represents fluorine, chlorine, bromine, cyano, nitro, C(O)NH2, C(S)NH2, trifluoromethyl, difluoromethyl, pentafluoroethyl, ethynyl, propyn-1-yl, 1-butyn-1-yl, pentyn-1-yl, or hexyn-1-yl, R3and R4independently of each other represent hydrogen, (C1-C8)-alkyl, R5represents hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, R13O-(C1-C8)-alkyl, (C2- C8)-alkenyl, aryl-(C1-C8)-alkyl, heteroaryl-(C1-C8)-alkyl, or heterocyclyl-(C1-C8)-alkyl, or R3and R5together with the carbon atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution, R6represents hydrogen, fluorine, chlorine, bromine, trifluoromethyl, difluoromethyl, methoxy, ethoxy, prop-1-yloxy, or but-1-yloxy, R7represents hydrogen, methyl, Q represents hydroxy or a group Q-1, Q-2R8represents hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R13, C(O)OR13, or (C1-C8)-alkoxy- (C1-C8)-alkyl, R9represents hydrogen, (C1-C8)-alkyl, R10represents hydrogen, halogen, cyano, nitro, (C1-C8)-alkyl, (C1-C8)-aaloalkyl, (C3-C8)-cycloalkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C3-C8)-halocycloalkyl, (C3-C8)- 130 US_ACTIVE\130153301\V-1halocycloalkyl-(C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, R11R12N- (C1-C8)-alkyl, R13O-(C1-C8)-alkyl, cyano-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy- (C1-C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)- alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, OR13, NR11R12, SR14, S(O)R14, SO2R14, R14S-(C1-C8)-alkyl, R14(O)S-(C1-C8)-alkyl, R14O2S- (C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)- alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl, bis- hydroxyboryl-(C1-C8)-alkyl, bis-[(C1-C8)-alkoxy]boryl-(C1-C8)-alkyl, tetramethyl-1,3,2- Dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1-C8)-alkyl, nitro-(C1-C8)- alkyl, C(O)OR13, C(O)R13, C(O)NR11R12, R13O(O)C-(C1-C8)-alkyl, R11R12N(O)C- (C1-C8)-alkyl, or bis-(C1-C8)-alkoxy-(C1-C8)-alkyl, or R8and R10together with the carbon atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution, R11and R12independently of each other represent hydrogen, (C1-C8)-alkyl, (C2-C8)- alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)-cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)- alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)-alkyl, (C1-C8)- alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)-cycloalkenyl-(C1-C8)-alkyl, C(O)R13, SO2R14, heterocyclyl, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)- alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)-alkoxycarbonyl, (C2-C8)- alkenyloxycarbonyl, (C2-C8)-alkynyloxycarbonyl, or heterocyclyl-(C1-C8)-alkyl, or R11and R12together with the nitrogen atom to which they are bonded form a fully saturated or partly saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution, 131 US_ACTIVE\130153301\V-1R13represents hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)- cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)- cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)- haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy- (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)- alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)- cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)- alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis- [(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl-amino-(C2-C6)-alkyl, aryl-(C1-C8)- alkyl-amino-(C2-C6)-alkyl, R14S-(C1-C8)-alkyl, R14(O)S-(C1-C8)-alkyl, R14O2S-(C1-C8)- alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris- [(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)-alkyl, [(C1-C8)- Alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1-C8)-alkyl, (C3-C8)- cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, aryloxy- (C1-C8)-alkyl, heteroaryloxy-(C1-C8)-alkyl, or (C1-C8)-alkoxycarbonyl, R14represents hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)- cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)- cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl- (C1-C8)-alkyl, (C4-C10)-cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]amino, (C1-C8)- alkylamino, aryl-(C1-C8)-amino, aryl-(C1-C6)-alkylamino, aryl-[(C1-C8)-alkyl]amino; (C3- C8)-cycloalkylamino, (C3-C8)-cycloalkyl-[(C1-C8)-alkyl]amino, N-azetidinyl, N- pyrrolidinyl, N-piperidinyl, or N-morpholinyl, and R15and R16independently of each other represent (C1-C8)-alkyl, (C3-C8)-cycloalkyl, aryl, heteroaryl, or heterocyclyl. 132 US_ACTIVE\130153301\V-1

[0239] Examples of the herbicidally active compounds within the scope of formula (III) include ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3- {2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro- 1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5- methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-propyl-4,5-dihydro-1,2- oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)- 3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[4- chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazol-3- yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5- dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro- 1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5- dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1- yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, 3-[4-chloro-2-fluoro-5- (5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5-dihydro-1,2-oxazol-3-yl)phenyl]-1,5- dimethyl-6-sulfanylidene-1,3,5-triazinane-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5- 133 US_ACTIVE\130153301\V-1dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2] oxazole-6a- carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2] oxazole-6a- carboxylate, and methyl 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH-cyclopenta[d][1,2] oxazole-6a- carboxylate.

[0240] 4) an herbicidally active compound corresponding to a compound selected from the group consisting of A1, A2, A3, A4, A5, A6, A7, A8 and A9, or an agrochemically acceptable salt thereof, wherein: A1 corresponds to:2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; A2 corresponds to:134 US_ACTIVE\130153301\V-1{[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid; A3 corresponds to:2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)- 3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; A4 corresponds to:1-ethoxy-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; A5 corresponds to:135 US_ACTIVE\130153301\V-12-{[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid; A6 corresponds to:1-methoxy-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; A7 corresponds to:1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; A8 corresponds to:136 US_ACTIVE\130153301\V-11-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate; and A9 corresponds to:1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate.

[0241] 5) an herbicidally active compound of the general formula (IV) or an agrochemically acceptable salt thereof V) whereinR1is hydrogen, fluoro, chloro, bromo, iodo, cyano, methyl, ethyl, prop-1-yl, 1- methylethyl, but-1-yl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, n- pentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1-dimethylpropyl, 1,2- dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, 1-methylpentyl, 2- methylpentyl, 3-methylpentyl, 4-methylpentyl, 1,1-dimethylbutyl, 1,2- dimethylbutyl, 1,3-di-methylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3- dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2- trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2-methylpropyl, trifluormethyl, difluormethyl, pentafluorethyl, 2,2-difluorethyl, 2,2,2-trifluorethyl, methoxy, 137 US_ACTIVE\130153301\V-1ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1- yloxy, 1,1-dimethyleth-1-yloxy, difluormethoxy, trifluormethoxy, pentafluorethoxy, 2,2-difluorethoxy, or 2,2,2-trifluorethoxy, R2is hydrogen, fluoro, chloro, bromo, methyl, trifluormethyl, methoxy, ethoxy, prop- 1-yloxy, or but-1-yloxy, R3is hydrogen, fluoro, chloro, bromo, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but-2-yloxy, 2-methylprop-1-yloxy, or 1,1-dimethyleth-1-yloxy, R4is fluoro, chloro, bromo, cyano, NO2, C(O)NH2, C(S)NH2, trifluormethyl, difluormethyl, pentafluorethyl, ethinyl, propin-1-yl, 1-butin-1-yl, pentin-1-yl, or hexin-1-yl, R5, R6and R7are independently from each other hydrogen, fluoro, chloro, bromo, iodo, cyano, methyl, ethyl, prop-1-yl, 1-methylethyl, but-1-yl, 1-methylpropyl, 2- methylpropyl, 1,1-dimethylethyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, 3- methylbutyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1- ethylpropyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4- methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-di-methylbutyl, 2,2- dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl, 1-ethyl-2- methylpropyl, trifluormethyl, difluormethyl, pentafluorethyl, 2,2-difluorethyl, 2,2,2-trifluorethyl, methoxy, ethoxy, prop-1-yloxy, prop-2-yloxy, but-1-yloxy, but- 2-yloxy, 2-methylprop-1-yloxy, 1,1-dimethyleth-1-yloxy, difluormethoxy, trifluormethoxy, pentafluorethoxy, 2,2-difluorethoxy, or 2,2,2-trifluorethoxy, G is methylene, (methyl)methylene, (ethyl)methylene, (prop-1-yl)methylene, (prop- 2-yl)methylene, (but-1-yl)methylene, (but-2-yl)methylene, (pent-1-yl)methylene, (pent-2-yl)methylene, (pent-3-yl)methylene, (dimethyl)methylene, (diethyl)methylene, ethylene, n-propylen, (1-methyl)ethyl-1-en, (2-methyl)ethyl-1- en, n-butylen, 1-methylpropyl-1-en, 2-methylpropyl-1-en, 3-methylpropyl-1-en, 1,1-dimethylethyl-1-en, 2,2-dimethylethyl-1-en, 1-ethylethyl-1-en, 2-ethylethyl-1- 138 US_ACTIVE\130153301\V-1en, 1-(prop-1-yl)ethyl-1-en, 2-(prop-1-yl)ethyl-1-en, 1-(prop-2-yl)ethyl-1-en, 2- (prop-2-yl)ethyl-1-en, 1,1,2-trimethylethyl-1-en, 1,2,2-trimethylethyl-1-en, 1,1,2,2-tetramethylethyl-1-en, n-pentylen, 1-methylbutyl-1-en, 2-methylbutyl-1- en, 3-methylbutyl-1-en, 4-methylbutyl-1-en, 1,1-dimethylpropyl-1-en, 2,2- dimethylpropyl-1-en, 3,3-dimethylpropyl-1-en, 1,2-dimethylpropyl-1-en, 1,3- dimethylpropyl-1-en, 1-ethylpropyl-1-en, n-hexylen, 1-methylpentyl-1-en, 2- methylpentyl-1-en, 3-methylpentyl-1-en, 4-methylpentyl-1-en, 1,1-dimethylbutyl- 1-en, 1,2-dimethylbutyl-1-en, 1,3-di-methylbutyl-1-en, 2,2-dimethylbutyl-1-en, 2,3-dimethylbutyl-1-en, 3,3-dimethylbutyl-1-en, 1-ethylbutyl-1-en, 2-ethylbutyl-1- en, 1,1,2-trimethylpropyl-1-en, 1,2,2-trimethylpropyl-1-en, 1-ethyl-1- methylpropyl-1-en, or 1-ethyl-2-methylpropyl-1-en, X and Y are independently from each other O (oxygen) or S (sulfur) and Q is one of the groups Q-1 to Q-25, wherein in the formulae of the following table the arrow stands for a bond of the respective group Q to the carbonyl group in the general formula (I):139 US_ACTIVE\130153301\V-1

[0242] An example of an herbicidally active compound within the scope of formula (IV) is cyclopropylmethyl-(2-{2-chlor-4-fluor-5-[3-methyl-2,6-dioxo-4-(trifluormethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}phenoxy)acetate, which has the following structure:140 US_ACTIVE\130153301\V-1

[0243] 6) an herbicidally active compound of the general formula (V) or an agrochemically acceptable salt thereof V)wherein W represents a group W-1 to W-2 O O 1 R 1 R A represents nitrogen or CH; R1represents hydrogen or methyl; R2represents hydrogen or fluorine; R3represents hydrogen, halogen, or (C1-C8)-alkoxy; R4represents halogen, cyano, NO2, C(O)NH2, C(S)NH2, (C1-C8)-haloalkyl, or (C2- C8)-alkynyl; R5, R6, and R7independently of one another represent hydrogen, halogen, cyano, (C1-C8)- alkyl, (C1-C8)-haloalkyl, (C1-C8)-alkoxy, or (C1-C8)-haloalkoxy; 141 US_ACTIVE\130153301\V-1G represents a branched or unbranched alkylene group optionally substituted with an (C1-C3)-alkoxy group; Q represents hydroxy or a group Q-1, Q-2;R8represents hydrogen, (C1-C8)-alkyl, (C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, (C2-C8)-alkynyl, (C2-C8)-alkenyl, C(O)R13, C(O)OR13, or (C1-C8)-alkoxy-(C1- C8)-alkyl; R9represents hydrogen or (C1-C8)-alkyl; R10represents hydrogen, halogen, cyano, NO2, (C1-C8)-alkyl, (C1-C8)-haloalkyl, (C3- C8)-cycloalkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C3-C8)-halocycloalkyl, (C3-C8)- halocycloalkyl-(C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, R11R12N- (C1-C8)-alkyl, R13O-(C1-C8)-alkyl, cyano-(C1-C8)-alkyl, (C1-C8)-alkylcarbonyloxy-(C1- C8)-alkyl, (C3-C8)-cycloalkylcarbonyloxy-(C1-C8)-alkyl, arylcarbonyloxy-(C1-C8)-alkyl, heteroarylcarbonyloxy-(C1-C8)-alkyl, heterocyclylcarbonyloxy-(C1-C8)-alkyl, OR13, NR11R12, SR14, S(O)R14, SO2R14, R14S-(C1-C8)-alkyl, R14(O)S-(C1-C8)-alkyl, R14O2S-(C1- C8)-alkyl, tris-[(C1-C8)-alkyl]silyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl](aryl)silyl(C1-C8)- alkyl, [(C1-C8)-alkyl]-bis-(aryl)silyl-(C1-C8)-alkyl, tris-[(C1-C8)-alkyl]silyl, bis- hydroxyboryl-(C1-C8)-alkyl, bis-[(C1-C8)-alkoxy]boryl-(C1-C8)-alkyl, tetramethyl-1,3,2- dioxaborolan-2-yl, tetramethyl-1,3,2-dioxaborolan-2-yl-(C1-C8)-alkyl, nitro-(C1-C8)- alkyl, C(O)OR13, C(O)R13, C(O)NR11R12, R13O(O)C-(C1-C8)-alkyl, R11R12N(O)C-(C1- C8)-alkyl, or bis-(C1-C8)-alkoxy-(C1-C8)-alkyl, or 142 US_ACTIVE\130153301\V-1R8and R10together with the carbon atom to which they are attached form a fully saturated or partially saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution; R11and R12are identical or different and independently of one another represent hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)-cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)-cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)- haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkylthio-(C1-C8)-alkyl, (C1-C8)-haloalkylthio-(C1-C8)- alkyl, (C1-C8)-alkoxy-(C1-C8)-haloalkyl, aryl, aryl-(C1-C8)-alkyl, heteroaryl, heteroaryl- (C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)-cycloalkenyl-(C1-C8)-alkyl, COR13, SO2R14, heterocyclyl, (C1-C8)-alkoxycarbonyl, bis-[(C1-C8)-alkyl]aminocarbonyl- (C1-C8)-alkyl, (C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl- aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxycarbonyl, heteroaryl-(C1-C8)- alkoxycarbonyl, (C2-C8)-alkenyloxycarbonyl, (C2-C8)-alkynyloxycarbonyl, or heterocyclyl-(C1-C8)-alkyl, or R11and R12together with the nitrogen atom to which they are attached form a fully saturated or partially saturated 3- to 10-membered monocyclic or bicyclic ring optionally interrupted by heteroatoms and optionally having further substitution; R13represents hydrogen, (C1-C8)-alkyl, (C2-C8)-alkenyl, (C2-C8)-alkynyl, (C1-C8)- cyanoalkyl, (C1-C10)-haloalkyl, (C2-C8)-haloalkenyl, (C3-C8)-haloalkynyl, (C3-C10)- cycloalkyl, (C3-C10)-halocycloalkyl, (C4-C10)-cycloalkenyl, (C4-C10)-halocycloalkenyl, (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-haloalkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)- haloalkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy- (C1-C8)-alkoxy-(C1-C8)-alkyl, (C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)-alkoxy-(C1-C8)- alkoxy-(C1-C8)-alkyl, aryl, aryl-(C1-C8)-alkyl, aryl-(C1-C8)-alkoxy-(C1-C8)-alkyl, heteroaryl, heteroaryl-(C1-C8)-alkyl, (C3-C8)-cycloalkyl-(C1-C8)-alkyl, (C4-C10)- cycloalkenyl-(C1-C8)-alkyl, bis-[(C1-C8)-alkyl]aminocarbonyl-(C1-C8)-alkyl, (C1-C8)- alkyl-aminocarbonyl-(C1-C8)-alkyl, aryl-(C1-C8)-alkyl-aminocarbonyl-(C1-C8)-alkyl, bis- [(C1-C8)-alkyl]amino-(C2-C6)-alkyl, (C1-C8)-alkyl amino-(C2-C6)-alkyl, aryl-(C1-C8)- 143 US_ACTIVE\130153301\V-1alkyl-amino-(C2-C6)-alkyl, R14S-(C1-C8)-alkyl, R14(O)S-(C1-C8)-alkyl, R14O2S-(C1-C8)- alkyl, hydroxycarbonyl-(C1-C8)-alkyl, heterocyclyl, heterocyclyl-(C1-C8)-alkyl, tris-[(C1- C8)-alkyl]sil...

Claims

CLAIMS 1. A recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:10; SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9; and a complete complement of any of the foregoing.

2. The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule is derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product comprising cotton event Gh_CSM63718, a representative sample of seed comprising the event having been deposited as ATCC Accession No. PTA-127638.

3. The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule is comprised in a cotton plant, seed, plant part, plant cell, or progeny plant comprising cotton event Gh_CSM63718, or a commodity product produced therefrom, a representative sample of seed comprising the event having been deposited as ATCC Accession No. PTA-127638.

4. The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule is formed by the insertion of a heterologous nucleic acid molecule into the genomic DNA of a cotton plant or cotton cell.

5. The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule comprises an amplicon diagnostic for the presence of cotton event Gh_CSM63718.

6. A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with cotton event Gh_CSM63718 DNA in a sample, wherein detecting hybridization of the DNA molecule under the stringent hybridization conditions is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.US_ACTIVE\130153301\V-13417. A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe specific for detecting in a sample at least one of: a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718; a 3’ junction sequence between the transgenic insert of cotton event Gh_CSM63718 and flanking cotton genomic DNA; SEQ ID NO:9; and a fragment of SEQ ID NO:9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO:9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718.

8. The DNA molecule of claim 6 or 7, wherein the DNA probe comprises SEQ ID NO:

21.

9. The DNA molecule of claim 6 or 7, wherein the DNA molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; and a complement of any of the foregoing.

10. A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein the first and the second DNA molecules are different from one another, and each comprise a fragment of SEQ ID NO:10 or a complement thereof and function as DNA primers when used together in an amplification reaction with DNA comprising cotton event Gh_CSM63718 to produce an amplicon diagnostic for cotton event Gh_CSM63718 in a sample.

11. The pair of DNA molecules of claim 10, wherein the first and the second DNA molecules comprise SEQ ID NO:19 and SEQ ID NO:

20.

12. The pair of DNA molecules of claim 10, wherein the first and the second DNA molecules comprise SEQ ID NO:19 and SEQ ID NO:

20.

13. The pair of DNA molecules of claim 10, wherein the amplicon comprises a nucleotide sequence selected from the group consisting of: SEQ ID NO:1;US_ACTIVE\130153301\V-1342SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; and a fragment of any of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, wherein the fragment is at least 10 nucleotides in length and comprises nucleotides 1,000–1,001 or 17,736-17,737 of SEQ ID NO:

10.

14. The DNA molecule of any one of claims 6–9 or the pair of DNA molecules of any one of claims 10–12, wherein the sample is derived from a cotton plant, seed, plant part, plant cell, progeny plant, or commodity product.

15. A method of detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part, plant cell, progeny plant, or commodity product, the method comprising: a) contacting the sample with the DNA molecule that functions as a DNA probe of any one of claims 6–9 and 13; b) subjecting the sample and the DNA molecule that functions as a probe to stringent hybridization conditions; and c) detecting the hybridization of the DNA molecule that functions as a probe to a DNA molecule in the sample,US_ACTIVE\130153301\V-1343wherein the hybridization of the DNA molecule that functions as a probe to the DNA molecule in the sample is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.

16. A method of detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part or plant cell, progeny plant or commodity product, the method comprising: a) contacting the sample with the pair of DNA molecules of any one of claims 10–13; b) performing an amplification reaction sufficient to produce a DNA amplicon; and c) detecting the presence of the DNA amplicon; wherein the DNA amplicon comprises at least one of: a 5’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718, a 3’ junction sequence between flanking cotton genomic DNA and the transgenic insert of cotton event Gh_CSM63718, SEQ ID NO: 9, and a fragment of SEQ ID NO: 9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO: 9 to identify the sequence as a fragment of the transgenic insert of Gh_CSM63718; and wherein the presence of the DNA amplicon indicates the presence of cotton event Gh_CSM63718 in the sample.

17. The method of claim 15, wherein the DNA amplicon is at least 10 nucleotides in length, at least 11 nucleotides in length, at least 12 nucleotides in length, at least 13 nucleotides in length, at least 14 nucleotides in length, at least 15 nucleotides in length, at least 16 nucleotides in length, at least 17 nucleotides in length, at least 18 nucleotides in length, at least 19 nucleotides in length, at least 20 nucleotides in length, at least 25 nucleotides in length, at least 30 nucleotides in length, at least 35 nucleotides in length, at least 40 nucleotides in length, at least 45 nucleotides in length, at least 50 nucleotides in length, at least 60 nucleotides in length, at least 70 nucleotides in length, at least 80 nucleotides in length, at least 90 nucleotides in length, or at least 100 nucleotides in length.US_ACTIVE\130153301\V-134418. The method of claim 15 or 16, wherein the DNA amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:10; SEQ ID NO:9; SEQ ID NO:8; SEQ ID NO:7; SEQ ID NO:6; SEQ ID NO:5; SEQ ID NO:4; SEQ ID NO:3; SEQ ID NO:2; SEQ ID NO:1; and a fragment of any of SEQ ID NO:10, SEQ ID NO:8, SEQ ID NO:7, SEQ ID NO:6, SEQ ID NO:5, SEQ ID NO:4, SEQ ID NO:3, SEQ ID NO:2, and SEQ ID NO:1 that is at least 10 nucleotides in length and comprises nucleotides 1,000–1,001 or 17,736-17,737 of SEQ ID NO:

10.

19. A method of detecting the presence of cotton event Gh_CSM63718 in a sample of DNA derived from a cotton seed, plant, plant part, plant cell, progeny plant or commodity product, the method comprising: a) contacting the sample with the DNA molecule that functions as a probe of any one of claims 6–9 and 13; and b) performing a sequencing reaction to produce a target sequence, wherein the target sequence comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; a complete complement of any thereof; and a fragment of any of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10 that is at least 10 nucleotides long and comprises nucleotides 1,000–1,001 or 17,736- 17,737 of SEQ ID NO:

10.

20. A method of detecting the presence of cotton event Gh_CSM63718 in a sample derived from a cotton seed, plant, plant part, cell, progeny plant or commodity product, the method comprising: a) contacting the sample with an antibody specific for the PPO protein encoded by cotton event Gh_CSM63718, an antibody specific for the TDO protein encoded by cotton event Gh_CSM63718, or a combination thereof; and b) detecting binding of the antibody or antibodies to the protein or proteins in the sample; wherein the binding of the antibody or antibodies indicates the presence of cotton event Gh_CSM63718 in the sample.

21. The method of claim 20, wherein the method further comprises:US_ACTIVE\130153301\V-1345a) contacting the sample with an antibody specific for the DMO protein encoded by cotton event Gh_CSM63718, an antibody specific for the EPSPS protein encoded by cotton event Gh_CSM63718, an antibody specific for the PAT protein encoded by cotton event Gh_CSM63718, or a combination of any thereof; and b) detecting binding of the antibody or antibodies to the protein or proteins in the sample; wherein the binding of the antibody or antibodies indicates the presence of cotton event Gh_CSM63718 in the sample.

22. A DNA detection kit for detecting the presence of cotton event Gh_CSM63718 in a sample, wherein the kit comprises: a) the pair of DNA primers of any one of claims 10–13; and / or b) the DNA molecule that functions as a probe of any one of claims 6–9 and 13.

23. A protein detection kit for detecting the presence of cotton event Gh_CSM63718 in a sample, wherein the kit comprises an antibody specific for the PPO protein encoded by cotton event Gh_CSM63718, an antibody specific for the TDO protein encoded by cotton event Gh_CSM63718, or a combination thereof; wherein detecting binding of the antibody or antibodies to the protein(s) encoded by cotton event Gh_CSM63718 in a sample is diagnostic for the presence of cotton event Gh_CSM63718 in the sample.

24. The protein detection kit of claim 22, wherein the kit further comprises an antibody specific for the DMO protein encoded by cotton event Gh_CSM63718, an antibody specific for the EPSPS protein encoded by cotton event Gh_CSM63718, an antibody specific for the PAT protein encoded by cotton event Gh_CSM63718, or a combination of any thereof.

25. A method of determining the zygosity of a cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718, the method comprising: a) contacting a sample comprising DNA derived from the cotton plant, plant part, plant seed, or plant cell with a first primer set capable of producing a first amplicon diagnostic for the presence of cotton event Gh_CSM63718, and a second primer set capable of producing a second amplicon diagnostic for wildtype cotton genomic DNA not comprising cotton event Gh_CSM63718;US_ACTIVE\130153301\V-1346b) performing a nucleic acid amplification reaction; and c) detecting the first amplicon and the second amplicon, wherein the presence of both amplicons indicates that the plant, plant part, seed or cell is heterozygous for cotton event Gh_CSM63718, and the presence of only the first amplicon indicates that the plant, plant part, seed, or cell is homozygous for cotton event Gh_CSM63718.

26. The method of claim 24, wherein the first primer set comprises SEQ ID NO:19 and SEQ ID NO:20, and the second primer set comprises SEQ ID NO:19 and SEQ ID NO:

22.

27. A method of determining the zygosity of a cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718, the method comprising: a) contacting a sample comprising DNA derived from the cotton plant, plant part, plant seed, or plant cell with a probe set comprising at least a first probe that specifically hybridizes to cotton event Gh_CSM63718, and at least a second probe that specifically hybridizes to cotton genomic DNA that was disrupted by insertion of the heterologous DNA of cotton event Gh_CSM63718 but does not hybridize to cotton event Gh_CSM63718; and b) hybridizing the probe set with the sample under stringent hybridization conditions, wherein detecting hybridization of only the first probe under the hybridization conditions is diagnostic for a cotton plant, plant part, seed or plant cell homozygous for cotton event Gh_CSM63718, and wherein detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a cotton plant, plant part, seed, or plant cell heterozygous for cotton event Gh_CSM63718.

28. The method of claim 26, wherein the probe set comprises SEQ ID NO:21 and SEQ ID NO:

23.

29. A DNA construct comprising a first expression cassette, a second expression cassette, a third expression cassette, a fourth expression cassette, and a fifth expression cassette, wherein: a) the first expression cassette comprises in operable linkage i) a ribulose bisphosphate carboxylase / oxygenase (RuBisCO) activase gene promoter, and a leader sequence from Arabidopsis thaliana, ii) a codon-optimized phosphinothricin N-acetyltransferase (PAT)US_ACTIVE\130153301\V-1347coding sequence from Streptomyces viridochromogenes, and iii) a 3’ UTR of a small heat shock protein (Hsp20) from Medicago truncatula; b) the second expression cassette comprises in operable linkage i) an enhancer from the strawberry vein banding virus (SVBV) fused to the promoter and 5’ UTR from a CAB1 (Chlorophyll A / B Binding Protein) gene from Cucumis melo, ii) a codon-optimized triketone dioxygenase (TDO) coding sequence from Oryza sativa, and iii) a 3’ UTR of a TMA7 (translation machinery associated 7) protein from Medicago truncatula; c) the third expression cassette comprises in operable linkage i) a polyubiquitin gene (UBQ10) promoter, a leader and an intron sequence from Arabidopsis thaliana, ii) an N-terminal chloroplast transit peptide coding sequence of APG6 (Albino and Pale Green 6) from Arabidopsis thaliana fused to a codon-optimized dicamba monooxygenase (DMO) coding sequence from Stenotrophomonas maltophilia; and iii) a 3’ UTR of an aluminum-induced Sali3-2 protein from Medicago truncatula; d) the fourth expression cassette comprises in operable linkage i) an enhancer of the 35S gene from Figwort Mosaic Virus (FMV), ii) a promoter, a leader sequence, and an intron sequence of the elongation factor 1A gene (ELF1a) from Arabidopsis thaliana, iii) an N-terminal chloroplast transit peptide of granule bound starch synthase I from Triticum aestivum fused to a codon optimized 5-enolpyruvylshikimate-3-phosphate synthase gene (EPSPS) from Agrobacterium sp strain CP4, and iv) a 3’ UTR of a ribulose 1,5-bisphosphate carboxylase small subunit E9 (rbcS-E9) gene from Pisum sativum; and e) the fifth expression cassette comprises in operable linkage i) an enhancer derived from multiple enhancer sequences from Arabidopsis thaliana, ii) a promoter sequence designed from multiple promoter sequences from Arabidopsis thaliana, iii) an intron and 5’ UTR for a cytochrome C oxidase subunit VIa gene from Arabidopsis thaliana fused a 5’ UTR designed from multiple 5’ UTR sequences from Arabidopsis thaliana, iv) an N-terminal chloroplast transit peptide coding sequence of APG6 (Albino and Pale Green 6) from Arabidopsis thaliana, with monocot codon usage, fused to the coding region of a protoporphyrinogen oxidase (PPO) gene from Enterobacter cloacae with codons optimized for cotton, and v) a 3’ UTR from the fiber FbLate-2 gene from Gossypium barbadense.US_ACTIVE\130153301\V-134830. The DNA construct of claim 28, wherein the DNA construct comprises SEQ ID NO:

9.

31. The DNA construct of claim 28 or 29, further comprising at the 5’ and / or 3’ end of said construct: a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14; and / or b) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:12 or SEQ ID NO:

15.

32. A DNA construct comprising a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the full length of SEQ ID NO: 9; and wherein the DNA construct comprises at the 5’ and / or 3’ end of said construct (i) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14; and / or (ii) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:15.US_ACTIVE\130153301\V-134933. The DNA construct of any one of claims 28–31, wherein the construct comprises at the 5’ end of said construct one or more nucleotide sequences selected from SEQ ID NOs:58–127.

34. The DNA construct of any one of claims 28–32, wherein the construct comprises at the 3’ end of said construct one or more nucleotide sequences selected from SEQ ID NOs:128–197.

35. A method for controlling or preventing weed growth in an area, the method comprising planting cotton comprising event Gh_CSM63718 in the area and applying an effective amount of at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and any combination thereof, to control weeds in the area without injury to the cotton or with less than about 10% injury to the cotton.

36. The method of claim 34, wherein applying the effective amount of at least one herbicide comprises applying at least two or more herbicides selected from the group consisting of glufosinate, a ß- triketone HPPD inhibitor, dicamba, glypohsate, a PPO herbicide, and any combination thereof over a growing season.

37. A method for controlling volunteer cotton comprising cotton event Gh_CSM63718 in an area, the method comprising applying an herbicidally effective amount of at least one herbicide other than glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, or a PPO herbicide, wherein the herbicide application prevents growth of cotton comprising cotton event Gh_CSM63718.

38. The method of claim 36, wherein the herbicide other than glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, or a PPO herbicide is selected from the group consisting of atrazine, topramezone, clopyralid, pyrithiobac, fluometuron, (3-(3,4-dichlorophenyl)-1,1- dimethylurea) (DCMU), 2,4-D, thidiazuron, dichlorprop-p 2-ethylhexyl ester, dichlorprop-p, trifloxysulfuron, paraquat, diquat, and combinations of any thereof.

39. A method of obtaining a seed of a cotton plant or a cotton plant that is tolerant to glufosinate, a ß- triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, or any combination thereof, the method comprising: a) obtaining a population of progeny seed or plants grown therefrom, at least one of which comprises cotton event Gh_CSM63718; andUS_ACTIVE\130153301\V-1350b) identifying at least a first progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718.

40. The method of claim 38, wherein identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 comprises: a) growing the progeny seed or plant to produce progeny plants; b) treating the progeny plants with an effective amount of at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof, and c) selecting a progeny plant that is tolerant to the at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof.

41. The method of claim 38 or 39, wherein identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 comprises detecting the presence of cotton event Gh_CSM63718 in a sample derived from the progeny seed or plant grown therefrom.

42. The method of any of claims 38–40, wherein identifying the progeny seed or plant grown therefrom that comprises cotton event Gh_CSM63718 comprises detecting the presence of at least one protein encoded by cotton event Gh_CSM63718 in a sample derived from the progeny seed or plant grown therefrom.

43. A method of improving tolerance to at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof in a cotton plant comprising: a) inserting the DNA construct of any one of claims 28–33 into the genome of a cotton cell; b) generating a cotton plant from the cotton cell; and c) selecting a cotton plant comprising the DNA construct.

44. The method of claim 42, wherein the selecting comprises treating the cotton cell or plant with an effective amount of at least one herbicide selected from the group consisting of glufosinate, a ß- triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof.US_ACTIVE\130153301\V-135145. A cotton plant, plant seed, plant part, or plant cell comprising a recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7; SEQ ID NO:8; SEQ ID NO:9; SEQ ID NO:10; a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9; and a complete complement of any of the foregoing.

46. The cotton plant, plant seed, plant part, or plant cell of claim 44, wherein the plant, plant seed, plant part, or plant cell expresses at least one herbicide tolerance gene selected from the group consisting of phosphinothricin N-acetyltransferase (PAT), triketone dioxygenase (TDO), dicamba monooxygenase (DMO), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), protoporphyrinogen oxidase (PPO), and any combination thereof.

47. The cotton plant, plant seed, plant part, or plant cell of claim 44 or 45, wherein the plant, plant seed, plant part, or plant cell is tolerant to at least one herbicide selected from the group consisting of glufosinate, ß-triketone HPPD inhibitors, dicamba, glyphosate, PPO herbicides, and combinations of any thereof.

48. A cotton plant, plant seed, plant part, or plant cell tolerant to at least one herbicide selected from the group consisting of glufosinate, ß-triketone HPPD inhibitors, dicamba, glyphosate, PPO herbicides, and combinations of any thereof, wherein the cotton plant, plant seed, plant part, or plant cell comprises the DNA construct of any one of claims 28–32.

49. The cotton plant, plant seed, plant part, or plant cell of any one of claims 44–47, wherein the plant, plant seed, plant part, or plant cell comprises cotton event Gh_CSM63718, a representative sample of seed comprising the event having been deposited under ATCC Accession No. PTA-127638.

50. The cotton plant, plant seed, plant part, or plant cell of any one of claims 44–48, wherein the plant, plant seed, plant part, or plant cell is further defined as a progeny plant of any generation of a cotton plant comprising cotton event Gh_CSM63718, or a cotton plant part, plant seed, or plant cell derived therefrom.US_ACTIVE\130153301\V-135251. A cotton plant, plant part, plant seed, or plant cell that comprises cotton event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638.

52. The cotton plant part of any one of claims 44–50, wherein the plant part comprises a microspore, pollen, an anther, an ovule, an ovary, a boll, a flower, an embryo, a stem, a bud, a node, a leaf, a root, or a callus.

53. The cotton plant, plant seed, plant part, or plant cell of any one of claims 44–51, wherein the cotton seed, plant, plant part, or cell is obtained by the method of any one of claims 38–43.

54. A cotton plant, plant cell, plant part, or plant seed comprising a recombinant DNA construct integrated in chromosome 21, wherein the recombinant DNA construct confers tolerance to at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and combinations of any thereof, and wherein the recombinant DNA construct is integrated in a position of said chromosome flanked by at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14; and / or (ii) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:

15.

55. The cotton plant, plant cell, plant part, or plant seed of claim 53, wherein the at least 50 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:14 comprise one or more nucleotide sequences selected from SEQ ID NOs:58-127.US_ACTIVE\130153301\V-135356. The cotton plant, plant cell, plant part, or plant seed of claims 53 or 54, wherein the at least 50 contiguous nucleotides of SEQ ID NO:12 or SEQ ID NO:15 comprise one or more nucleotide sequences selected from SEQ ID NOs:128-197.

57. The cotton plant, plant cell, plant part, or plant seed of any one of claims 46–55, or the method of any one of claims 34, 35, and 38–43, wherein the ß-triketone HPPD inhibitor is selected from the group consisting of mesotrione, benzobicyclon (BBC), tembotrione, sulcotrione, tefuryltrione, and combinations of any thereof, and wherein the PPO herbicide is selected from the group consisting of diphenylethers, N-phenylphthalimides, oxadiazoles, oxazolidinediones, phenylpyrazoles, pyrimidinediones, thiadiazoles, triazolinones, benzoxazinone derivatives, other PPO herbicides, and combinations of any thereof.

58. The cotton plant, plant cell, plant part, or plant seed, or method of claim 56, wherein the diphenylether is selected from the group consisting of acifluorfen, bifenox, ethoxyfen, fluorodifen, fluoronitrofen, furyloxyfen, halosafen, chlomethoxyfen, chlornitrofen, ethoxyfen-ethyl, fluoroglycofen, lactofen, nitrofen, oxyfluorfen, fomesafen, a salt of any thereof, and an ester of any thereof; the N-phenylphthalimide is selected from the group consisting of cinidon-ethyl, flumiclorac, flumiclorac-pentyl, and flumioxazin; the oxadiazole is selected from the group consisting of oxadiargyl and oxadiazon; the oxazolidinedione is pentoxazone; the phenylpyrazole is selected from the group consisting of fluazolate, pyraflufen, and pyraflufen-ethyl; the pyrimidinedione is selected from the group consisting of benzfendizone, butafenacil, epyrifencacil (S-3100), flupropacil, flufenoximacil, saflufenacil, and tiafenacil; the thiadiazole is selected from the group consisting of fluthiacet-methyl and thidiazimin; the triazolinone is selected from the group consisting of azafenidin, bencarbazone, carfentrazone, its salts and esters, and sulfentrazone; the benzoxazinone derivative is 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3,4-dihydro-3-oxo-4- prop-2-ynyl-2H-1,4-benzoxazin-6-yl)-1,3,5-triazinane-2,4-dione (trifludimoxazin)); or the other PPO herbicide is selected from the group consisting of chlorphthalim, flufenpyr, flufenpyr-ethyl, flumipropyn, pyraclonil, profluazol, pyridin-2-ylmethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2- methoxyethyl [(3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-cyano-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-US_ACTIVE\130153301\V-1354yl]phenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2-bromo-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}pyridin-2-yl)oxy]acetate; cyclopropylmethyl (2-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}phenoxy)acetate; methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} propanoate (flufenoximacil), methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} propanoate, methyl 2- {[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(Z)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, ethyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, ethyl (2R)-2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoate, ethyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]benzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl- 2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}propanoic acid, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}-2-methylpropanoate, ethyl 2-{[(E)-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}-2-methylpropanoate, methyl 2-{[(E)-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoate, methyl (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} butanoate, methyl (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy} butanoate, 2-{[(E)-{2-chloro-4-fluoro-5-[3-US_ACTIVE\130153301\V-1355methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoic acid, (2R)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6- dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, (2S)-2-{[(E)-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]benzylidene}amino]oxy}butanoic acid, ethyl 2-{[(E)-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]benzylidene}amino]oxy}butanoate, methyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate methyl (2R)-2- ({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoate, methyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, 2-({(E)- [2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoic acid, (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoic acid, ethyl 2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, ethyl (2R)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorobenzylidene]amino}oxy)propanoate, ethyl (2S)-2-({(E)-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorobenzylidene]amino}oxy)propanoate, methyl 2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoate, methyl (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, methyl (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorobenzylidene}amino]oxy} propanoate, 2-{[(E)-{5- [3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, (2R)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, (2S)-2-{[(E)-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorobenzylidene}amino]oxy}propanoic acid, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-US_ACTIVE\130153301\V-13564-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole- 5-carboxylate, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5- dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, ethyl (5S)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl (5R)-3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin- 1(2H)-yl]phenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5- [3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-propyl-4,5- dihydro-1,2-oxazole-5-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-5-ethyl-4,5-dihydro-1,2-oxazole-5- carboxylate, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5- dihydro-1,2-oxazol-3-yl)phenyl]-1-methyl-6-(trifluoromethyl)pyrimidine-2,4(1H,3H)-dione, ethyl 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, methyl 3-[2-chloro-5-(3,5- dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro- 1,2-oxazole-5-carboxylate, 3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5R)-3-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5- methyl-4,5-dihydro-1,2-oxazole-5-carboxylic acid, (5S)-3-[2-chloro-5-(3,5-dimethyl-2,6-dioxo- 4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenyl]-5-methyl-4,5-dihydro-1,2-oxazole-5- carboxylic acid, 3-[4-chloro-2-fluoro-5-(5-{[(isopropylideneamino)oxy]carbonyl}-5-methyl-4,5- dihydro-1,2-oxazol-3-yl)phenyl]-1,5-dimethyl-6-sulfanylidene-1,3,5-triazinane-2,4-dione, ethyl 3-{5-[3-amino-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4- fluorophenyl}-5-methyl-4,5-dihydro-1,2-oxazole-5-carboxylate, 3-{5-[3-amino-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]-2-chloro-4-fluorophenyl}-5-methyl-4,5- dihydro-1,2-oxazole-5-carboxylic acid, methyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-US_ACTIVE\130153301\V-1357(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, ethyl 3-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, methyl 3-{2-bromo-4-fluoro-5-[3-methyl-2,6-dioxo- 4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenyl}-3a,4,5,6-tetrahydro-6aH- cyclopenta[d][1,2] oxazole-6a-carboxylate, 2-ethoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, {[(1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropyl)carbonyl]oxy}acetic acid, 2-methoxy-2-oxoethyl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6- dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, ethyl [(3-{2-chloro-5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2- yl)oxy]acetate, [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin- 1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetic acid, ethyl (2-{2-chloro-5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}phenoxy)acetate, (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}phenoxy)acetic acid, ethyl (2-{2-chloro-4-fluoro- 5-[4-(1-fluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}phenoxy)acetate, 2-methoxyethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl- 2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, tetrahydrofuran-2-ylmethyl [(3-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, cyanomethyl [(3-{2- chloro-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}pyridin-2-yl)oxy]acetate, methyl (2-{2-chloro-5-[4-(1,1-difluoroethyl)-3-methyl- 2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}phenoxy)(methoxy)acetate, methyl (2-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}phenoxy)(methoxy)acetate, [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6- dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetic acid, ethyl [(3- {2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4- fluorophenoxy}pyridin-2-yl)oxy]acetate, 2-methoxyethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)- 3-methyl-2,6-dioxo-3,6-dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate,US_ACTIVE\130153301\V-1358tetrahydrofuran-2-ylmethyl [(3-{2-bromo-5-[4-(1,1-difluoroethyl)-3-methyl-2,6-dioxo-3,6- dihydropyrimidin-1(2H)-yl]-4-fluorophenoxy}pyridin-2-yl)oxy]acetate, ethyl 2-[[3-[5-[4-(1,1- difluoroethyl)-3-methyl-2,6-dioxo-pyrimidin-1-yl]-4-fluoro-2-nitro-phenoxy]- 2- pyridyl]oxy]acetate, 1-ethoxy-1-oxopropan-2-yl 1-{2-chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4- (trifluoromethyl)-3,6-dihydropyrimidin-1(2H)-yl]phenoxy}cyclopropanecarboxylate, 2-{[(1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropyl)carbonyl]oxy}propanoic acid, 1-methoxy-1-oxopropan-2-yl 1-{2- chloro-4-fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-{2-chloro-4- fluoro-5-[3-methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-ethoxy-1-oxobutan-2-yl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, 1-(ethoxycarbonyl)cyclopropyl 1-{2-chloro-4-fluoro-5-[3- methyl-2,6-dioxo-4-(trifluoromethyl)-3,6-dihydropyrimidin-1(2H)- yl]phenoxy}cyclopropanecarboxylate, ,2-ethoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, [({1-[2- chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)oxy]acetic acid, 1-ethoxy-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-[({1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4- sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropyl}carbonyl)oxy]propanoic acid, allyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-ethoxy-2-methyl-1-oxopropan-2-yl 1-[2-chloro-5- (3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 2-methoxy-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl- 2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 2- (dimethylamino)-2-oxoethyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5- triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6- dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4-fluorophenoxy]cyclopropanecarboxylic acid, methyl 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropanecarboxylate, 1-[2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-US_ACTIVE\130153301\V-13591,3,5-triazinan-1-yl)-4-fluorophenoxy]-N,N-dimethylcyclopropanecarboxamide, and ethyl 1-({1- [2-chloro-5-(3,5-dimethyl-2,6-dioxo-4-sulfanylidene-1,3,5-triazinan-1-yl)-4- fluorophenoxy]cyclopropyl}carbonyl)cyclopropanecarboxylate.

59. The method of any one of claims 34, 35, 39–41, 43, 56, and 57, wherein the effective amount of glufosinate is about 0.4 lb / acre to about 1.6 lb / acre over a growing season; wherein the ß-triketone HPPD inhibitor comprises mesotrione and the effective amount of mesotrione is about 0.09 lb / acre to about 0.36 lb / acre; wherein the effective amount of dicamba is about 0.5 lb / acre to about 2 lb / acre over a growing season; wherein the effective amount of glyphosate is about 0.5 lb / acre to about 2.5 lb / acre over a growing season; and wherein the effective amount of the PPO herbicide is about 0.0009 lb / acre to about 1.5 lb / acre over a growing season.

60. A method of producing a progeny cotton plant comprising cotton event Gh_CSM63718 comprising: a) sexually crossing a first cotton plant that comprises cotton event Gh_CSM63718 with itself or a second cotton plant; b) collecting one or more seeds produced from the cross; c) growing one or more seeds to produce one or more progeny plants; and d) selecting at least a first progeny plant or seed comprising cotton event Gh_CSM63718.

61. An inbred or hybrid cotton plant or seed comprising cotton event Gh_CSM63718 produced by the method of claim 59.

62. A nonliving or nonregenerable cotton plant material comprising the recombinant DNA molecule of any one of claims 1–4 or the DNA construct of any one of claims 28–33.

63. A nonliving or nonregenerable cotton plant material comprising cotton event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638.

64. A commodity product comprising the recombinant DNA molecule of any one of claims 1–4 or the DNA construct of any one of claims 28–33.US_ACTIVE\130153301\V-136065. The commodity product of claim 63, wherein the commodity product is produced from a transgenic cotton plant, plant part, plant seed, or plant cell comprising cotton event Gh_CSM63718.

66. The commodity product of claims 63 or 64, wherein the commodity product comprises whole or processed seeds; viable or nonviable seeds; viable plant parts (such as roots, nodes, bolls, buds or leaves); viable plant cells; processed plant parts; processed plant tissues; dehydrated plant tissues; dehydrated plant parts; frozen plant tissues; frozen plant parts; food for human consumption such as cottonseed oil; plant parts processed for animal feed such as cottonseed meal and cottonseed hulls; cotton fiber; or cotton linters.

67. A method of producing a commodity product, the method comprising: a) obtaining a transgenic cotton plant, plant part, or plant seed comprising cotton event Gh_CSM63718; and b) producing a commodity product from the transgenic cotton plant, plant part, or plant seed.

68. A method of controlling, preventing, or reducing the development of herbicide-tolerant weeds comprising cultivating in a crop growing environment a cotton plant comprising transgenes that provide tolerance to glufosinate, ß-triketone HPPD inhibitor herbicides, dicamba, glyphosate and PPO herbicides.

69. A method for controlling, preventing, or reducing the development of herbicide-tolerant weeds comprising: a) cultivating in a crop growing environment a cotton plant comprising the DNA construct of any one of claims 28–33 or event Gh_CSM63718; and b) applying to the crop growing environment at least one herbicide selected from the group consisting of glufosinate, a ß-triketone HPPD inhibitor, dicamba, glyphosate, a PPO herbicide, and any combination thereof, wherein the cotton plant is tolerant to the at least one herbicide.

70. The method of claim 67 or 68, wherein the transgenes that provide tolerance to the herbicides are present at a single genomic location in the cotton plant.US_ACTIVE\130153301\V-136171. A method of reducing loci for cotton breeding by inserting a construct comprising transgenes that provide tolerance to glufosinate, ß-triketone HPPD inhibitor herbicides, dicamba, glyphosate and PPO herbicides as a single locus at a genomic location in a cotton plant.

72. The cotton plant, plant cell, plant part, or plant seed of any one of claims 44–57 and 60, the nonliving or nonregenerable cotton plant material of claim 61 or 62, the commodity product of any one of claims 63–65, or the method of any one of claims 34–37, 42, 43, 56–60, and 66–70, wherein the cotton plant, plant cell, plant part, plant seed, nonliving or nonregenerable cotton plant material, or commodity product, further comprises one or more transgenes for providing resistance to insect infestations.

73. The cotton plant, plant cell, plant part, plant seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of claim 71, wherein the transgenes for providing resistance to insect infestations are selected from the group consisting of Cry1B.3, Cry1Da_7, Vip3Cb1.1, Cry2Ab2, and combinations of any thereof.

74. The cotton plant, plant cell, plant part, seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of claim 71 or 72, wherein the Cry1B.3 transgene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:225; the Cry1Da_7 transgene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:227; the Vip3Cb1.1 transgene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:229; and the Cry2Ab2 transgene comprises a polynucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:

231.

75. The cotton plant, plant cell, plant part, seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of any one of claims 71–73, wherein the cotton plant, plant cell, plant part, plant seed, nonliving or nonregenerable plant material, or commodity product further comprises cotton event Gh_BCS246002 and / or cotton event MON15947.

76. The cotton plant, plant cell, plant part, seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of any of claims 71–74, wherein the transgenes for providing resistance to insect infestations provide resistance to infestations by Lepidopteran pests selected from the group consisting of Cotton Bollworm (Helicoverpa zea), Tobacco BudwormUS_ACTIVE\130153301\V-1362(Heliothis virescens), Fall Armyworm (Spodoptera frugiperda), Old World Bollworm (Helicoverpa armigera), and combinations of any thereof.

77. The cotton plant, plant cell, plant part, plant seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of any of claims 71–75, wherein the transgenes in Gh_BCS246002 for providing resistance to insect infestations are present at a single genomic location in the cotton plant.

78. The cotton plant, plant cell, plant part, seed, the nonliving or nonregenerable cotton plant material, the commodity product, or the method of any one of claims 71–76, wherein the cotton plant, plant cell, plant part, seed further comprises a recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:212; SEQ ID NO:213; SEQ ID NO:214; SEQ ID NO:215; SEQ ID NO:216; SEQ ID NO:217; SEQ ID NO:218; SEQ ID NO:219; SEQ ID NO:220; SEQ ID NO:221; a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:212 or the full length of SEQ ID NO: 213; and a complete complement of any of the foregoing.

79. A cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product, comprising a foreign DNA at an insertion site in the cotton genome, the insertion site having a nucleic acid sequence at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the sequence of SEQ ID NO:

13.

80. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 78, wherein the foreign DNA is flanked by 5’ and 3’ flanking regions, wherein said 5’ flanking region is upstream of and contiguous with said foreign DNA and comprises SEQ ID NO:11, and wherein said 3’ flanking region is downstream of and contiguous with said foreign DNA and comprises SEQ ID NO:

12.

81. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 78 or 79, wherein the foreign DNA comprises at least oneUS_ACTIVE\130153301\V-1363herbicide tolerance gene selected from the group consisting of a phosphinothricin N- acetyltransferase (PAT) gene, a triketone dioxygenase (TDO) gene, a dicamba monooxygenase (DMO) gene, a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, a protoporphyrinogen oxidase (PPO) gene, and any combination of any thereof.

82. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 80, wherein the foreign DNA comprises a phosphinothricin N-acetyltransferase (PAT) gene, a triketone dioxygenase (TDO) gene, a dicamba monooxygenase (DMO) gene, a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, and a protoporphyrinogen oxidase (PPO) gene.

83. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of any one of claims 78–81, wherein the foreign DNA comprises SEQ ID NO:

9.

84. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of any one of claims 78–81, wherein the foreign DNA comprises SEQ SEQ ID NO:9 with one or more modifications.

85. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 83, wherein the one or more modifications comprise one or more insertions, one or more deletions, one or more substitutions, or a combination of any thereof, within SEQ ID NO:

9.

86. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 84, comprising a deletion of all or a portion of the phosphinothricin N-acetyltransferase (PAT) gene, a deletion of all or a portion of the triketone dioxygenase (TDO) gene, a deletion of all or a portion of the dicamba monooxygenase (DMO) gene, a deletion of all or a portion of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, a deletion of all or a portion of the protoporphyrinogen oxidase (PPO) gene, or a combination of any thereof.

87. The cotton plant, plant cell, plant part, seed, nonliving or nonregenerable cotton plant material, or cotton commodity product of claim 84 or 85, comprising an insertion of an additional expression cassette.US_ACTIVE\130153301\V-136488. A method of producing a cotton plant or seed, said method comprising inserting foreign DNA at an insertion site in the genome of the cotton plant or seed, the insertion site having a nucleic acid sequence at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the sequence of SEQ ID NO:

13.

89. The method of claim 87, wherein the cotton plant produced by the method has improved trait efficacy, increased expression of one or more transgenes, improved stability of one or more transgenes, improved agronomic characteristics, or any combination thereof, as compared to a plant that has the same foreign DNA inserted at a different insertion site in the genome.

90. A method of producing a cotton plant or seed, said method comprising obtaining a cotton plant or seed comprising cotton event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638, and inserting foreign DNA into the sequence of SEQ ID NO:10 of the plant or seed.

91. A method of producing a cotton plant or seed, said method comprising obtaining a cotton plant or seed comprising event Gh_CSM63718, a representative sample of seed comprising cotton event Gh_CSM63718 having been deposited under ATCC Accession No. PTA-127638, and deleting all or a portion of the sequence of SEQ ID NO:10 in the plant or seed.

92. A cotton plant or seed produced by the method of any one of claims 87–90.

93. A method of detecting the presence a nucleic acid molecule encoding PPO_H_N90 in a sample derived from a cotton seed, plant, plant part or plant cell, progeny plant, or commodity product, the method comprising: a) contacting the sample with a pair of DNA molecules; b) performing an amplification reaction sufficient to produce a DNA amplicon comprising the nucleic acid encoding the PPO_H_N90 or a portion of the nucleic acid encoding the PPO_H_N90 of sufficient length to identify the presence of the nucleic acid encoding the PPO_H_N90; and c) detecting the presence of the DNA amplicon;US_ACTIVE\130153301\V-1365wherein the presence of the DNA amplicon indicates the presence of PPO_H_N90 in the sample.

94. The method of claim 92, wherein the pair of DNA molecules comprises SEQ ID NO:234 and SEQ ID NO:

235.

95. A method of detecting the presence of a nucleic acid molecule encoding PPO_H_N90 in a sample derived from a cotton seed, plant, plant part, plant cell, progeny plant, or commodity product, the method comprising: contacting the sample with a DNA molecule that functions as a DNA probe specific for the nucleic acid molecule encoding PPO_H_N90; subjecting the sample and the DNA molecule that functions as a probe to stringent hybridization conditions; and detecting the hybridization of the DNA molecule that functions as a probe to a DNA molecule in the sample, wherein the hybridization of the DNA molecule that functions as a probe to the DNA molecule in the sample is diagnostic for the presence of PPO_H_N90 in the sample.

96. The method of claim 94, wherein the DNA molecule that functions as a probe comprises SEQ ID NO:236.US_ACTIVE\130153301\V-1366

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