Triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and preparation method of triple antigen and antibody complex

A swine viral diarrhea, antibody complex technology, applied in the direction of virus antigen components, biochemical equipment and methods, viruses/phages, etc. Safe and convenient, fast immune effect, high protection rate

Active Publication Date: 2017-01-04
TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vaccines need a certain amount of time to generate an immune response, and emergency disease cannot immediately produce protection; antibiotics are not effective against viral d

Method used

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  • Triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and preparation method of triple antigen and antibody complex
  • Triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and preparation method of triple antigen and antibody complex
  • Triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and preparation method of triple antigen and antibody complex

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example 1: Antigen antibody complex I

[0040] A triple antigen-antibody complex of porcine viral diarrhea, the antigen-antibody complex comprising an antigen and an antibody, and the antigen is a triple virus liquid obtained by mixing porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus, The antibody is a triple yolk antibody prepared from the antigen.

[0041] The volume ratio of the antigen and the antibody in the antigen-antibody complex is 1:1.

[0042] In the triple antigen-antibody complex of porcine viral diarrhea, the virulence value of each virus is 1×10 6.0 TCID 50 Above, the neutralization titer of each egg yolk antibody reaches 1:7TCID 50 the above.

[0043] The volume ratio of porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus in the triple virus solution is 1:1:1.

[0044] The porcine transmissible gastroenteritis virus is a Chinese strain of porcine transmissible gastroe...

Example Embodiment

[0068] Example 2: Antigen-antibody complex II

[0069] On the basis of Example 1, the antigen-antibody complex further includes squalene, and the volume ratio of squalene to antigen and antibody is 1:2:2. The preparation method of the antigen-antibody complex is the same as in Example 1, and will not be repeated here.

[0070] The porcine viral diarrhea triple antigen-antibody complex I obtained in Example 1 and the porcine viral diarrhea triple antigen-antibody complex II obtained in Example 2 were tested as follows:

[0071] Test 1, immune test

[0072] Take 40 non-immune 3-day-old piglets and randomly divide them into four groups with 10 pigs in each group. The specific operation is as follows:

[0073] Blank control group: each piglet was injected with 2ml saline.

[0074] Vaccine control group: Each piglet is injected with 2ml of conventional triple vaccine group (2ml is the injection volume of conventional vaccine).

[0075] Antigen-antibody complex I group: each piglet was injecte...

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Abstract

The invention provides a triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and a preparation method of the triple antigen and antibody complex. The triple antigen and antibody complex comprises an antigen and an antibody, wherein the antigen is a triple virus liquid obtained by mixing porcine transmissible gastroenteritis viruses, porcine epidemic diarrhea viruses and porcine rotavirus, and the antibody is a triple egg yolk antibody prepared on the basis of the antigen. The triple antigen and antibody complex for preventing and controlling porcine viral diarrhea is convenient and quick to use and capable of safely and effectively preventing and controlling the porcine viral diarrhea. The triple antigen and antibody complex can effectively reduce the body immunological stress response, increases the protection production speed, rapidly increases the body antibody titer and is prevented from the virus diffusion risk in production and use processes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a porcine viral diarrhea triple antigen-antibody complex and a preparation method thereof. Background technique [0002] At present, porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus diarrhea are the three most harmful viral diarrhea diseases in pig herds in my country, which have seriously affected the development of pig industry. [0003] Porcine transmissible gastroenteritis is caused by porcine transmissible gastroenteritis virus. It is an infectious disease characterized by pig vomiting, diarrhea, and dehydration. It has a high lethality rate for newborn piglets; porcine epidemic diarrhea is porcine epidemic diarrhea A porcine intestinal infectious disease caused by a virus, characterized by watery diarrhea, vomiting and dehydration, pigs of all ages are susceptible, especially suckling piglets are the most severely affected, main...

Claims

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Application Information

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IPC IPC(8): A61K39/42A61K39/39A61K39/295A61K39/225A61K39/215A61K39/15A61P31/14
CPCA61K39/12A61K39/39A61K39/42A61K2039/5252A61K2039/55511A61K2039/70C12N2720/12334C12N2770/20034A61K2300/00
Inventor 王宇鹏杨雪王建程雪娇李丽琴王勇朱士江姜淋洁崔志刚王猛焦晓军季昆张立会余贵菊于小婷甄盼盼杜宁宁范庆增李玲
Owner TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
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