Solid fermentation culture medium for phellinus igniarius mycelium and solid fermentation cultural method for phellinus igniarius mycelium

A technology of solid medium and solid fermentation, which is applied in the direction of fertilizer mixture, fertilization device, application, etc., can solve the problems of low mycelium yield and long fruiting body culture period, achieve short culture time, be easy to put into large-scale production, and facilitate The effect of farmers or individuals promoting production

Inactive Publication Date: 2012-06-13
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current Phellinus culture mainly includes liquid submerged fermentation culture and bag material cultivation to obtain mycelia. However, du

Method used

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  • Solid fermentation culture medium for phellinus igniarius mycelium and solid fermentation cultural method for phellinus igniarius mycelium
  • Solid fermentation culture medium for phellinus igniarius mycelium and solid fermentation cultural method for phellinus igniarius mycelium

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Embodiment Construction

[0022] 1. Obtaining liquid strains:

[0023] (1) Liquid medium formula: 20g glucose, 1.5g peptone, 0.46gKH 2 PO 4 , 1.0gK 2 HPO 4 and 0.5gMgSO 4 , After mixing evenly, adjust the pH to 7.0 and set aside.

[0024] (2) Medium preparation: Put the prepared liquid medium into shaker flasks, 100mL per bottle, seal with a film, autoclave at 121°C for 20 minutes, and then put the sterilized medium into the ultra-clean work In Taichung, use an ozone generator to sterilize for 20 minutes.

[0025] (3) Inoculation: Inoculate a piece of bacteria with a diameter of about 5 mm in each bottle of culture medium ( Phellinus linteus ), according to the requirements of aseptic operation during the operation.

[0026] (4) Cultivation: Let the inoculated strains stand for one day, put them in a shaker the next day, and cultivate them for 8-12 days to obtain liquid strains for use.

[0027] 2. Operation steps of solid fermentation culture:

[0028] (1) Preparation of nutrient solution: w...

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Abstract

The invention discloses a solid fermentation medium for phellinus igniarius mycelium and a solid fermentation cultural method for the phellinus igniarius mycelium, belonging to the technical field of phellinus igniarius artificial culture. Corn flour, wheat bran and the like are used as materials for preparing culture medium which can be used for the solid fermentation culture medium for the phellinus igniarius mycelium. Compared with the current phellinus igniarius mycelium liquid fermentation and phellinus igniarius solid cultural method, the cultural method can acquire much phellinus igniarius mycelium while economical and convenient to operate; moreover, the content of polysaccharide and flavone is higher than that of crude polysaccharides and flavone in each gram of sporophore.

Description

technical field [0001] The invention belongs to the technical field of the artificial cultivation of Phellinus fungus. Background technique [0002] The medicinal use of Sanghuang was first recorded in Li Shizhen's "Compendium of Materia Medica". Slightly bitter taste of Phellinus Phellinus, can benefit the five internal organs, soften and harden, detoxify, stop bleeding, promote blood circulation, etc. It is recorded in "Medicinal Properties of Medicine" that Phellinus Phellinus is slightly bitter in taste and cold in nature. It is used in traditional Chinese medicine to treat dysentery, night sweats, metrorrhagia, blood stranguria, astringent pain in the navel and abdomen, prolapse of the anus, diarrhea, and amenorrhea. [0003] According to reports, Phellinus is the medicinal fungus with the highest anti-tumor ability at present. The anti-cancer function of Phellinus was first discovered by Japanese scholar Ikekawa et al. in 1968. The study showed that the extract of Phe...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 蒋宁刘红锦陈忠立孙道权储呈平诸永志王道营刘芳张苏珍王萍
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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