Soybean, corn and phellinus igniarius healthy food, and preparation method thereof
A technology of Phellinus linteus and corn, applied in the directions of food preparation, botanical equipment and methods, food science, etc., can solve the problems such as the lack of healthy food, the low content of Phellinus linteus mycelium, etc., to enhance immunity, enrich nutrition, reduce cost effect
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Embodiment 1
[0030] A preparation method of soybean and corn Phellinus Phellinus health food comprises the following steps:
[0031] (1) Preparation of Phellinus liquid mycelium ball:
[0032] A. Activate the Phellinus phellifolia strains with PDA medium, compound, and culture in a constant temperature incubator at 28°C for 10 days, until the mycelia cover the slope, obtain the Phellinus PDA slant strains, and put them in the refrigerator for subsequent use;
[0033] B, insert Phellinus PDA slant bacterial classification into shaker culture medium and carry out expansion culture, obtain liquid fermentation primary production bacterial classification;
[0034] Shaker medium formula: soybean flour 10 g / L, corn flour 20 g / L, wheat bran powder 1 g / L, yeast powder 1 g / L, KH 2 PO 4 0.1 g / L, ZnSO 4 ·7H 2 O 0.1 g / L, VB 1 0.1g / L, pH natural;
[0035] Culture conditions: temperature 26°C, liquid volume 1500 mL / 5000 mL, rotation speed 100 r / min, culture for 8 days;
[0036] C, the first-leve...
Embodiment 2
[0047] A preparation method of soybean and corn Phellinus Phellinus health food comprises the following steps:
[0048] (1) Preparation of Phellinus liquid mycelium ball:
[0049] A. Activate the Phellinus phellifolia strains with PDA medium, compound, and culture in a constant temperature incubator at 28°C for 10 days, until the mycelia cover the slope, obtain the Phellinus PDA slant strains, and put them in the refrigerator for subsequent use;
[0050] B, insert Phellinus PDA slant bacterial classification into shaker culture medium and carry out expansion culture, obtain liquid fermentation primary production bacterial classification;
[0051] Shaker medium formula: soybean flour 30 g / L, corn flour 30 g / L, wheat bran flour 10 g / L, yeast powder 10 g / L, KH 2 PO 41.0 g / L, ZnSO 4 ·7H 2 O 1.0 g / L, VB 1 0.3 g / L, natural pH;
[0052] Culture conditions: temperature 30°C, liquid volume 2500 mL / 5000 mL, rotation speed 150 r / min, culture for 10 days;
[0053] C, the first-lev...
Embodiment 3
[0064] A preparation method of soybean and corn Phellinus Phellinus health food comprises the following steps:
[0065] (1) Preparation of Phellinus liquid mycelium ball:
[0066] A. Activate the Phellinus phellifolia strains with PDA medium, compound, and culture in a constant temperature incubator at 28°C for 10 days, until the mycelia cover the slope, obtain the Phellinus PDA slant strains, and put them in the refrigerator for subsequent use;
[0067] B, insert Phellinus PDA slant bacterial classification into shaker culture medium and carry out expansion culture, obtain liquid fermentation primary production bacterial classification;
[0068] Shaker medium formula: soybean flour 20 g / L, corn flour 25 g / L, wheat bran flour 5 g / L, yeast powder 5 g / L, KH 2 PO 4 0.5g / L, ZnSO 4 ·7H 2 O 0.5 g / L, VB 1 0.2 g / L, pH natural;
[0069] Culture conditions: temperature 28°C, liquid volume 2000 mL / 5000 mL, rotation speed 120 r / min, culture for 9 days;
[0070] C, the first-level...
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