Method for purifying concentrated polysaccharide of male agaric by using isolation technique of composition filmes

Abstract

This invention discloses a method for purifying and concentrating Phellinus igniarius polysaccharide by combined membrane separation technique. The method comprises: (1) culturing in liquid to obtain Phellinus igniarius polysaccharide fermented solution; (2) performing solid-liquid separation with gauze or frame filter cloth; (3) separating the fermented solution with non-grade ceramic membrane system to obtain clear Phellinus igniarius polysaccharide solution; (4) purifying and concentrating with an organic ultrafiltration membrane system with molecular weight of 5000 grade to obtain concentrated Phellinus igniarius polysaccharide solution; (5) freeze-drying or spray-drying to obtain Phellinus igniarius polysaccharide dry powder. The method avoids the use of organic solvent of ethanol, and has such advantages as high separation and concentration rate, low energy consumption, no pollution, low cost and short time, thus is suitable for mass production.

Description

Technical field: [0001] The invention belongs to the downstream technical field of bioengineering, and is a new technology for rapidly separating and concentrating the fermented liquid of the medicinal fungus Phellinus phellidis to prepare Phellinus polysaccharide. Background technique [0002] Phellinus linteus (Phellinus linteus), also known as Phellinus linteus, Mulberry, Sanchen, etc., belongs to Basidiomycetes, Phellinus genus, parasitizes on poplar, willow, pine and other trees, and is a new type of rare food. Medicinal fungi have medicinal effects such as significantly improving the body's immunity and anti-tumor activity, and are of great development value. The medicinal use was first included in Li Shizhen's "Compendium of Materia Medica", which has the functions of benefiting the five internal organs, promoting intestinal qi, hemostasis, softening and detoxifying, and is used in the treatment of hepatitis and cancer. Among them, the main medicinal activity of Phel...

AI Technical Summary

Problems solved by technology

[0003] At present, there is a great demand for Phellinus product in domestic and foreign markets, but due to the particularity and complexity of Phellinus Phellinus physiology and ecology, the number of Phellinus polysaccharide fruiting bodies is very rare, and it is necessary to industrially produce Phellinus polysaccharide active substance by using large-scale liquid culture technology. An effective way to meet the market demand, the domestic patent reports on Phellinus liquid culture aspects include: "Phellinus mycelium product and its production method and use" invented by Zhang Weimin et al. (application number 01127760.2); invented by Ao Zonghua et al. "Large-scale liquid submerged fermentation for the production of Phellinus water extract and polysaccharide technology" (Patent No. 200410013869.6), but there is no patent report on the downstream technology of extracting and preparing polysaccharide products with high purity and high biological activity from Phellinus fermented liquid

[0004] At present, the industrial extraction of polysaccharides from medicinal fungi mainly adopts the method of ethanol precipitation after evaporation and concentration. The polysaccharides are precipitated at low temperature (4°C). This process requires a large amount of alcohol and power costs, and at the same time brings a large amount of difficult-to-treat ethanol process wastewater. , causing a certain degree of pollution to the environment, the evaporative concentration process also consumes a lot of energy, and because the high temperature will reduce the biological activity of polysaccharides, it is difficult to achieve clean production, high activity and high purity by using the traditional process of evaporative concentration and ethanol precipitation demand for polysaccharide biological products