Phellinus igniarius strain and culturing method for same

A cultivation method and Phellinus technology, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of harsh cultivation conditions, no research reports on strains, and difficulties in artificial cultivation, etc., and achieve fast growth, high growth Exuberant, the effect of meeting market demand

Inactive Publication Date: 2015-09-02
INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the growth of Phellinus Phellinus requires a relatively special natural climate environment, the number of natural Phellinus Phellinus is very scarce, and artificial cultivation is extremely difficult, the cultivation conditions are harsh, and the growth cycle is as long as 3-4 years, which is difficult to meet the growing market. need
This application is a strain found and isolated from Phellinus sanghuang strain SH21011 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp.nov) on mulberry trees. After searching, there is no information about this strain research report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: A Phellinus species, found and separated from Phellinus species SH21011 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp.nov) on mulberry trees, and its classification is named Inonotus sanghuang, preserved The number is CGMCC No.6395.

[0018] The culture method of above-mentioned Phellinus bacterium classification, it may further comprise the steps:

[0019] S1. Prepare culture medium

[0020] S11. Weigh potatoes, bran, glucose, and agar according to the proportion of the medium formula, and set aside for later use. The medium formula is mainly composed of the following components in weight ratio: 150g of potatoes, 40g of bran, 20g of glucose, and 20g of agar;

[0021] S12. Put potatoes and bran in a beaker, add 1000 mL of water, heat and boil for 30 minutes, filter with gauze, and transfer the filtrate to another beaker;

[0022] S13. Add agar to the filtrate, heat and boil until the agar is completely dissolved, then add glucose, stir until the ...

Embodiment 2

[0027] Example 2: A Phellinus species is discovered and isolated from Phellinus species SH21011 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp.nov) on mulberry trees, and the preservation number is CGMCC No.6395.

[0028] The culture method of above-mentioned Phellinus bacterium classification, it may further comprise the steps:

[0029] S1. Prepare culture medium;

[0030] S11. Weigh potatoes, bran, glucose, and agar according to the proportion of the medium formula, and set aside; wherein, the medium formula is mainly composed of the following components by weight: 250g of potatoes, 80g of bran, 25g of glucose, and 23g of agar;

[0031] S12. Put potatoes and bran in a beaker, add 1500mL of water, heat and boil for 40min, filter with gauze, and transfer the filtrate to another beaker;

[0032] S13. Add agar to the filtrate, heat and boil until the agar is completely dissolved, then add glucose, stir until the glucose is completely dissolved, adjust the pH to 6, ad...

Embodiment 3

[0037] Example 3: A Phellinus species, found and separated from Phellinus species SH21011 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp.nov) on mulberry trees, and its classification is named Inonotus sanghuang, preserved The number is CGMCC No.6395.

[0038] The culture method of above-mentioned Phellinus bacterium classification, it may further comprise the steps:

[0039] S1. Prepare culture medium

[0040] S11. Weigh potatoes, bran, glucose, and agar according to the proportion of the medium formula, and set aside; wherein, the medium formula is mainly composed of the following components in weight ratio: 180g of potatoes, 55g of bran, 22g of glucose, and 21g of agar;

[0041] S12. Put potatoes and bran in a beaker, add 1200mL of water, heat and boil for 35min, filter with gauze, and transfer the filtrate to another beaker;

[0042] S13. Add agar to the filtrate, heat and boil until the agar is completely dissolved, then add glucose, stir until the glucose is...

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Abstract

The invention discloses a phellinus igniarius strain and a culturing method for the same. The strain is classified and named as Inonotus sanghuang, and is preserved with a preservation number CGMCC No.6395. The culturing method for the strain comprises the following steps of 1, preparing a culture medium; 2, performing inoculation, namely extracting a phellinus igniarius strain block in n aseptic environment by virtue of an inoculation tool, transferring the phellinus igniarius strain block into the middle part of a test tube culture medium, and plugging the test tube culture medium with cotton seeds; 3, performing strain culturing, namely transferring a test tube inoculated in step 2 into a constant temperature incubator, and performing constant temperature culturing for 10 to 15 days at 25 to 35 DEG C in a darkroom. According to the phellinus igniarius strain and the culturing method for the same, the phellinus igniarius strain cultured by the method is discovered and separated from a phellinus igniarius strain SH21022 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp,nov) on mulberries, and grows vigorously and rapidly, and large-scale mycelium fermentation production, active ingredient extraction and healthcare product or medicament development can be realized to meet market requirements.

Description

technical field [0001] The invention relates to a strain and a cultivation method thereof, in particular to a Phellinus strain and a cultivation method thereof. Background technique [0002] Phellinus igniarius is Basidiomycotina, Hymenomycetes, Polyporales, Hymenochaetacae, Phellinus fungus, is a kind of Precious large medicinal fungi. Traditional Chinese medicine believes that Phellinus japonica is sweet in nature and bitter in taste, and is used to treat metrorrhagia, prolapse of the anus, leukorrhea, amenorrhea, and diarrhea due to spleen deficiency. Modern pharmacological studies have shown that Phellinus has anti-tumor, immune-enhancing, anti-hepatic fibrosis and other effects. The main active ingredients of Phellinus are polysaccharides, triterpenoids and brass substances. Among them, flavonoids have anti-oxidation, blood vessel expansion, blood lipid lowering and anti-cancer effects, and are generally extracted from natural plants. Phellinus is a rare medicinal f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05G3/00
Inventor 王波
Owner INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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