41results about How to "No change in biological activity" patented technology

The invention relates the separating agent box and application. The agent box comprises erythrocyte precipitating agent, hypaque sodium- ficoll 400# or HISTOPAQUE1077 and cell maintenance liquid. The agent box can separate bone marrow and umbilical cord blood, on the surface of cell there is no any mark, and it doesn't change biological activity of cell. The invention can commercially manufacture, and the application is wide.

A preparation method of a micro bubble ultrasound contrast agent coated by a microcapsule relates to a preparation method of a micro bubble ultrasound contrast agent. The invention solves the problems of the instability of a micro bubble contrast agent and the disappearance of acoustic characteristic of a modified micro bubble. The preparation method of the micro bubble ultrasound contrast agent is that: absorbing, standing or centrifuging, cleaning, absorbing once again, standing or centrifuging and cleaning once more; finally the micro bubble ultrasound contrast agent is available. The invention has simple process, mild reaction condition, simple operation, good reproducibility, wild range of application and environment-friendliness, and is applicable to the surface modification of various ultrasound contrast agents and can be easily implemented.

The invention discloses a method for cultivating hepatitis e virus. The method comprises the steps that a serum of a later pregnant woman is added into an HEB strain obtained through separation of an epidemic disease investigation for in-vitro culture, the virus can be replicated massively, and immunofluorescence and Western Blot analysis are utilized for revealing that the serum of the later pregnant woman can promote massive replication of HEV progeny virus. According to the method, great breakthrough is made in the aspect of HEV in-vitro culture, and a good foundation is laid for further researching the biological and immunological characteristics of HEV and the HEV infection and pathogenic mechanism and screening and identifying anti-HEV drugs.

The invention discloses a method for preserving bone tissue materials. The method for preserving the bone tissue materials comprises the steps that (1) the bone tissue materials are cleaned; (2) the bone tissue materials are placed into a povidone iodine preservation solution with mass concentration of 0.01%-0.5%, the mixture is stored in refrigeration equipment, and the storage temperature is lower than 0 DEG C; and (3) the bone tissue materials stored in a refrigerated mode and the immersion liquid are transferred to the refrigeration, and the mixture is taken out after thawing, the mixtureis soaked by 0.9% physiological saline, a ringer's solution or phosphate buffer and then cleaned, and the mixture can be used as a bone graft tissue. The preservation solution uses sodium bicarbonateor the phosphate buffer as a pH adjuster such that a pH value of the preservation solution is between 5.8 and 8.0. According to a cryopreservation bag, the side edge of a part of the bag body is openthe bag body is connected with a flow guiding hose, and the outer end of the hose is closed. According to the method for preserving the bone tissue materials, the integrity of a bone tissue structureis maintained, and the mechanical properties are kept unchanged; and organic components and inorganic components of the bone tissue materials are not lost, so that the suffering and economic burden ofthe patient are alleviated.

A method for analyzing carbohydrate-binding protein with a functional carbohydrate chip and a time-of-flight mass spectrometer comprises the following steps of: preparing a coupling agent solution, incubating solid carrier, cleaning, drying, dissolving reducing carbohydrate in water, adjusting to required concentration, spotting, and incubating to obtain a functional carbohydrate chip; directly adding a biological sample on the functional carbohydrate chip, incubating, removing impurities, forming crystal, dissociating crystal, detecting the flight time of charging ions in a vacuum electric field with a time-of-flight mass spectrometer, obtaining a protein map that directly shows molecular weight, abundance and other information of proteins in the detected biological sample, and identifying protein differential expression; and comparing the protein map information of the detected biological sample with a sample map information to obtain the information of carbohydrate-binding protein with differential expression. The invention solves the technical problems in the prior art, including low detection throughput, complex detection procedures, and long detection period. The invention has the advantages of simple operation, high sensitivity and good repeatability; and is suitable for rapid analysis in medical clinics.

The invention discloses a method for extracting nicotine through steam distillation and acid absorption. The method comprises the following steps: crushing a tobacco raw material, adding the crushed tobacco raw material into a feeder together with an alkali liquid, and mixing so as to obtain an alkali-containing material; conveying the alkali-containing material into a steam distillation tank, wherein the alkali-containing material and steam inversely moves to be mixed, so as to obtain steam with nicotine; introducing steam with nicotine into an acid absorbing liquid in a jacket type enamel kettle so that the acid absorbing liquid absorbs nicotine till the acid absorbing liquid is saturated, discharging out, separating, and purifying, thereby obtaining a nicotine extract; reabsorbing the gas generated inside the jacket enamel kettle by using a columnar enamel cylinder, and discharging the gas into a steam distillation tank for recycling. By adopting the method, nicotine can be effectively separated from other components in tobacco, the biological activity of solanesol in residues can be kept unchanged, and industrial production of a nicotine product can be achieved.

The invention discloses a staphylococcus lysozyme local sustained release preparation, a preparation method and application thereof. The preparation comprises the following components in percentage by weight: 1 to 10 percent of staphylococcus lysozyme, 1 to 10 percent of mannite, 0 to 90 percent of polylactic acid (PLA), and 5 to 95 percent of polylactide-co-glycolide (PLGA). The preparation takes the staphylococcus lysozyme as a bactericidal active component to obtain polylactide-co-glycolide (PLA-PLGA) polymer microspheres containing the staphylococcus lysozyme through supercritical fluid microparticle preparation technology. The preparation has good physical and chemical properties and strong sterilization effect, has adjustable time during the in vitro medicament release, ensures that the medicament release process can reach three days to three months, and has steady release rate.

Disclosed is a deletion mutant of a recombinant human nerve growth factor. The mutant is a peptide chain of the complete human nerve growth factor, with 3 amino-acids at C-terminal deleted, wherein the amino-acid sequence of the mutant is as shown in sequence list SEQ ID No.1, or the amino-acid sequence of the mutant is an amino-acid sequence that shares over 95% homology with that which is shown in SEQ ID No.1 and has the biological activity of a nerve growth factor. The mutant in the invention retains the C-terminal integrity in rhNGF by the strategy of expressing rhNGF with 3 amino-acids deleted at C-terminal (rhNGF-D3), so the expression level of rhNGF-D3 protein in eukaryotic cells is increased five- to ten-fold by this modification compared to rhNGF, with the same bioactivity and uniform protein C-terminal.

The invention discloses an angelica anomala polysaccharide extracted on the basis of response surface optimization and a preparation method and an application thereof. The molecular weight of the angelica anomala polysaccharide is 1.17kDa; after angelica anomala as a medicinal material undergoes water extraction, alcohol precipitation, deproteinization and desalination, chromatographic separation is then carried out by a DEAE-agarose gel FF column and an agarose gel 6FF gel column, and thereby the angelica anomala polysaccharide is obtained; and the conditions of water extraction are as follows: extraction is carried out under 81 DEG C for three times, extraction is carried out for one hour each time, and the dosage ratio of water to angelica anomala powder is 68mL/g. The angelica anomala polysaccharide prepared by the invention has good bacteriostatic activity, oxidation resistance and whitening activity. The method disclosed by the invention has the advantages of high extraction rate and good purification effect, and does not change the structure of the angelica anomala polysaccharide and affect the bioactivity of the angelica anomala polysaccharide.

The invention discloses corn noodles prepared by utilizing corn in a dough stage and a production method thereof. The corn noodles comprise the following ingredient and auxiliary ingredient constituents in a mass ratio: corn flour in the dough stage 15%-25%, flour 60%-70%, salt 0.5%-2%, vital wheat gluten flour 5%-10% and soybean powder 5%-8%. The corn noodles are prepared through the operation steps of preparing corn juice and corn the corn flour with the corn in the dough stage, performing mixing, performing dough kneading, performing fermentation, , performing rolling, performing slicing, performing drying, performing cutting, etc. According to the invention, through utilizing the characteristic of complementary physical properties and nutrients of the corn in the dough stage, soybeans, etc., the adopted production method provided by the invention guarantees that the biological activity of nutritional health care components is not damaged to the greatest extent; and the corn noodles have certain health care efficacy.

The invention discloses multi-grain noodles containing kudzu vine roots. The noodles are prepared from the following raw materials in parts by mass: 46-50 parts of wheat flour, 13-15 parts of kudzu vine root flour, 2-4 parts of konjac powder, 6-8 parts of corn flour, 8-10 parts of red long bean flour, 5-10 parts of vital gluten and 1-2 parts of edible salt. The preparation method comprises the steps of preparation of partial raw materials, kneading, curing, mixing, rolling, slitting, drying, cutting off, packaging and the like. According to the preparation method, the biological activity of nutritional ingredients in the raw materials can be maintained by selecting local specialty, such as kudzu vine root flour, konjac powder, red long bean flour, corn flour and the like, as raw materialsand a corresponding preparation method, no essence or other chemical substance is added, the taste and flavor of noodles are improved by adding the kudzu vine root flour and the konjac powder, the noodles are chewable and flexible due to the reasonable prescription, the nutritional value and edible therapy effect of the noodles are improved, and human health is favored.

The invention discloses a process of enriching nutritional components of sea cucumber processing liquid. The process comprises the following steps: filtering and centrifuging the sea cucumber processing liquid, adding NaHCO3, Na2CO3 and a mixed solution, adding a HCl solution of 0.2 to 0.5 moL/L, putting into a PDMS membrane bioreactor, and performing pervaporization to obtain concentrated liquid;and sterilizing the concentrated liquid by a pasteurization method and performing cold storage at 4 DEG C for later use. The sea cucumber processing liquid is treated by the process provided by the invention, so that the nutritional components of the sea cucumber processing liquid can be enriched and recycled, the discharged liquid of the treated sea cucumber processing liquid is condensed watervapor, and pollution to the surrounding environment is reduced.

The invention relates to the technical field of astaxanthin, in particular to an efficient preparation and purification method of natural free astaxanthin. The preparation and purification method comprises the following steps of (1) performing raw material selection and pretreatment; (2) constructing a haematococcus pluvialis enzymolysis system: dispersing lipase and an antioxidant into deionized water and/or a buffer solution, and then adding the deionized water and/or the buffer solution system containing the lipase and the antioxidant into a haematococcus pluvialis pretreatment solution obtained in the step (1) to obtain a haematococcus pluvialis oil enzymolysis system; (3) performing primary enzymolysis; (4) performing primary enrichment; (5) performing secondary enzymolysis; and (6) performing secondary enrichment. The method is simple and easy to operate, low in cost, mild in condition and environmentally friendly; the content of the free astaxanthin in a finally prepared product reaches 60% or above; and the generation of byproducts in the enzymolysis and enrichment processes of the astaxanthin can be effectively avoided.