Method for preserving bone tissue materials

Abstract

The invention discloses a method for preserving bone tissue materials. The method for preserving the bone tissue materials comprises the steps that (1) the bone tissue materials are cleaned; (2) the bone tissue materials are placed into a povidone iodine preservation solution with mass concentration of 0.01%-0.5%, the mixture is stored in refrigeration equipment, and the storage temperature is lower than 0 DEG C; and (3) the bone tissue materials stored in a refrigerated mode and the immersion liquid are transferred to the refrigeration, and the mixture is taken out after thawing, the mixtureis soaked by 0.9% physiological saline, a ringer's solution or phosphate buffer and then cleaned, and the mixture can be used as a bone graft tissue. The preservation solution uses sodium bicarbonateor the phosphate buffer as a pH adjuster such that a pH value of the preservation solution is between 5.8 and 8.0. According to a cryopreservation bag, the side edge of a part of the bag body is openthe bag body is connected with a flow guiding hose, and the outer end of the hose is closed. According to the method for preserving the bone tissue materials, the integrity of a bone tissue structureis maintained, and the mechanical properties are kept unchanged; and organic components and inorganic components of the bone tissue materials are not lost, so that the suffering and economic burden ofthe patient are alleviated.

Description

technical field [0001] The invention belongs to the technical field of biological material processing, in particular to a method for preserving bone tissue materials. Background technique [0002] In clinical medical technology work, especially in craniocerebral surgery, orthopedics, orthopedics, stomatology, otolaryngology, maxillofacial plastic surgery and bone tumors, in order to maintain the speed of bone window fusion after surgery and reduce rejection The occurrence of the reaction meets the patient's psychological expectation of "returning things to their original owners" and eliminates anxiety. Under possible conditions, more and more medical workers and patients actively use autologous bone materials to treat postoperative tissue defects. Perform replanting. In this clinical method, the non-destructive and active preservation of autologous bone tissue materials becomes an important factor for the success of replantation and the degree of recovery of the diseased bo...

AI Technical Summary

Problems solved by technology

[0004] The technical research of the present invention is to overcome the various methods for wet preservation of bone tissue materials at present. Defects in maintaining the biocompatibility, surface activity and biodegradability of bone materials well, resulting in the situation that it can only be used for short-term storage in actual use and cannot be stored for a long time

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