Method for analyzing carbohydrate-binding protein by functional sugar chip and flight mass spectrometer

Abstract

A method for analyzing carbohydrate-binding protein with a functional carbohydrate chip and a time-of-flight mass spectrometer comprises the following steps of: preparing a coupling agent solution, incubating solid carrier, cleaning, drying, dissolving reducing carbohydrate in water, adjusting to required concentration, spotting, and incubating to obtain a functional carbohydrate chip; directly adding a biological sample on the functional carbohydrate chip, incubating, removing impurities, forming crystal, dissociating crystal, detecting the flight time of charging ions in a vacuum electric field with a time-of-flight mass spectrometer, obtaining a protein map that directly shows molecular weight, abundance and other information of proteins in the detected biological sample, and identifying protein differential expression; and comparing the protein map information of the detected biological sample with a sample map information to obtain the information of carbohydrate-binding protein with differential expression. The invention solves the technical problems in the prior art, including low detection throughput, complex detection procedures, and long detection period. The invention has the advantages of simple operation, high sensitivity and good repeatability; and is suitable for rapid analysis in medical clinics.

Description

technical field [0001] The invention relates to a method for analyzing sugar-binding proteins, in particular to a method for detecting and identifying sugar-binding proteins by using a functional sugar chip combined with surface-enhanced laser analysis ionization time-of-flight mass spectrometry technology. Background technique [0002] At present, the techniques involved in the analysis and detection of proteomics mainly include: two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), electrospray mass spectrometry (ESI-MS), matrix-assisted laser desorption ion time-of-flight mass spectrometry (MALDI-TOF). -MS) and the recently emerging technology of protein chip coupled with time-of-flight mass spectrometry. Time-of-flight mass spectrometer is developed based on surface-enhanced laser desorption ionization spectrometry (surface-enhanced laser desorption ionization spectrometry, SELDI). In the same year, Bill Hutchens and Tai-Yung Yip of the United States proposed an...

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a method for analyzing sugar-binding proteins with a functional sugar chip and a flight mass spectrometer, which solves the technical problems of low detection throughput, cumbersome detection steps and long cycle in the background technology