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Preparation method of astaxanthin-rich haematococcus pluvialis oil

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of preparation of astaxanthin-rich Haematococcus pluvialls oil, can solve the problems of low astaxanthin content, achieve simple preparation methods, broad market prospects, and conditions mild effect

Inactive Publication Date: 2021-11-05
RIZHAO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low astaxanthin content in Haematococcus pluvialis oil currently on the market, the present invention provides a preparation method of astaxanthin-rich Haematococcus pluvialls oil to solve the above problems

Method used

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  • Preparation method of astaxanthin-rich haematococcus pluvialis oil

Examples

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Effect test

Embodiment 1

[0029] A preparation method of astaxanthin-rich Haematococcus pluvialis oil, the specific preparation steps are as follows:

[0030] (1) Raw material and pretreatment

[0031] Take 10 g of Haematococcus pluvialis oil, add 3 g of absolute ethanol therein, stir and mix well, and obtain the Haematococcus pluvialls oil pretreatment solution.

[0032] (2) Preparation of enzymatic hydrolysis system

[0033] Disperse 4 g of lipase powder and 0.8 g of ascorbic acid in 65 g of deionized water, then add the deionized aqueous solution containing lipase and ascorbic acid to the Haematococcus pluvialis pretreatment solution obtained in step (1) to obtain red globule pluvialls Algal oil enzymatic hydrolysis system.

[0034] (3) Enzymatic treatment

[0035] The Haematococcus pluvialis oil enzymatic hydrolysis system obtained in step (2) was subjected to enzymatic hydrolysis treatment under the condition of a water bath at 45°C. The enzymatic hydrolysis time was 5 hours, and the process wa...

Embodiment 2

[0039] A preparation method of astaxanthin-rich Haematococcus pluvialis oil, the specific preparation steps are as follows:

[0040] (1) Raw material and pretreatment

[0041] Take 10 g of Haematococcus pluvialis oil, add 2 g of absolute ethanol therein, stir and mix well, and obtain the Haematococcus pluvialls oil pretreatment liquid.

[0042] (2) Preparation of enzymatic hydrolysis system

[0043] Lipase powder 3g and sodium edetate 0.5g are dispersed in 60g deionized water, then the deionized aqueous solution containing lipase and sodium edetate is added to the Haematococcus pluvialis that step (1) obtains In the pretreatment solution, the Haematococcus pluvialis oil enzymatic hydrolysis system is obtained.

[0044] (3) Enzymatic treatment

[0045] The Haematococcus pluvialis oil enzymatic hydrolysis system obtained in step (2) was subjected to enzymatic hydrolysis treatment under the condition of a water bath at 50°C. The enzymatic hydrolysis time was 7 hours, and the p...

Embodiment 3

[0049] A preparation method of astaxanthin-rich Haematococcus pluvialis oil, the specific preparation steps are as follows:

[0050] (1) Raw material and pretreatment

[0051] Take 10 g of Haematococcus pluvialis oil, add 3 g of absolute ethanol therein, stir and mix well, and obtain the Haematococcus pluvialls oil pretreatment solution.

[0052] (2) Preparation of enzymatic hydrolysis system

[0053] Lipase powder 4g and sodium edetate 0.8g are dispersed in 65g deionized water, then the deionized aqueous solution containing lipase and sodium edetate is added to the Haematococcus pluvialis that step (1) obtains In the pretreatment solution, the Haematococcus pluvialis oil enzymatic hydrolysis system is obtained.

[0054] (3) Enzymatic treatment

[0055] The Haematococcus pluvialis oil enzymatic hydrolysis system obtained in step (2) was subjected to enzymatic hydrolysis treatment under the condition of a water bath at 45°C. The enzymatic hydrolysis time was 5 hours, and the...

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Abstract

The invention relates to a preparation method of astaxanthin-rich haematococcus pluvialis oil, and belongs to the technical field of compound extraction. The method comprises the following steps of (1) pretreating raw materials; (2) preparing an enzymolysis system; (3) performing enzymolysis treatment; and (4) performing enrichment process. According to the method, the enrichment of astaxanthin in haematococcus pluvialis oil is efficiently realized by adopting a method of combining enzymolysis with heterogeneous purification, and the content of total astaxanthin substances in a finally prepared product is more than 40%, so that the dosage requirement in the application process of the product is well met.

Description

technical field [0001] The invention belongs to the technical field of compound extraction, and in particular relates to a preparation method of astaxanthin-rich Haematococcus pluvialis oil. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4-diketo-β,β'-carotene) is a red ketone carotenoid found in some aquatic animals. According to reports, the antioxidant activity of astaxanthin is more than 10 times higher than that of β-carotene, and more than 500 times higher than that of vitamin E. It is called "super vitamin E". Astaxanthin can be used as a coloring agent in precious aquatic products and poultry farming to increase its additional commodity value. In addition, the human body can also selectively accumulate astaxanthin, which has anti-oxidation, anti-aging, anti-tumor, and prevention of cardiovascular and cerebrovascular diseases, etc. effect. Therefore, it has received extensive attention in recent years. [0003] Astaxanthin and its extracts from natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12P7/64C07C403/24
CPCC12P23/00C12P7/6463C07C403/24C07C2601/16
Inventor 周庆新陈芳甜谷彩霞王岳琦胡莘琳
Owner RIZHAO POLYTECHNIC
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