Angelica anomala polysaccharide extracted on basis of response surface optimization and preparation method and application thereof

A response surface methodology, the technology of Sichuan Angelica, applied in the field of biomedicine, can solve the problems of changing the biological activity of polysaccharides, reducing the activity of natural polysaccharides, and reducing the size of polysaccharides, and achieves good bacteriostatic activity, reduced starch content, and increased sugar content. Effect

Active Publication Date: 2017-08-25
SICHUAN AGRI UNIV
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  • Description
  • Claims
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Problems solved by technology

However, the effects of these extraction aids are limited. When the extraction system reaches equilibrium, the influence of a single condition change on the yield of polysaccharides will be small to insignificant
In addition, while improving the dissolution rate of polysaccharides, these methods will also affect the structure of polysaccharides, thereby changing the biological activity of polysaccharides, resulting in the reduction or loss of the activity of some natural polysaccharides
[0005] At present, the research on polysaccharides of Angelica dahurica is not deep enough
Only a few scholars have analyzed the extraction of polysaccharides from Angelica dahurica, anti-oxidation and enhancing the immune function of animal skin tissue, and have not yet conducted research on the optimization of the hot water extraction method of polysaccharides from Angelica dahurica and the differences in the structure and activity of polysaccharides obtained by different extraction methods

Method used

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  • Angelica anomala polysaccharide extracted on basis of response surface optimization and preparation method and application thereof
  • Angelica anomala polysaccharide extracted on basis of response surface optimization and preparation method and application thereof
  • Angelica anomala polysaccharide extracted on basis of response surface optimization and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Investigation on optimization of hot water extraction polysaccharides from Angelica dahurica by response surface methodology

[0051] Based on the single factor experiment, the Box-Behnken method of Design-expert 8.0.7.1Trail software was used to design the experiment, and the polysaccharide yield of Radix Angelica dahurica was used as the response value to obtain the optimal extraction conditions.

[0052] 1. Single factor test

[0053] Take the root of Baizhi root, wash it, dry it at 40°C, and crush it through a 40-mesh sieve. Weigh the powder sample of Angelica dahurica, add petroleum ether at 55°C to reflux for 4 hours, dry at 40°C, then add 80% ethanol to extract at 80°C for 4 hours to remove part of the pigment and oligosaccharides, evaporate the ethanol to dry, pulverize, and dry at 45°C Dry and set aside. The consumption of sherwood oil is calculated as 3mL by every gram of Chuan Baizhi powder, and the consumption of 80% ethanol is calculated as 2mL per gram o...

Embodiment 2

[0085] Preparation of Polysaccharides from Radix Angelica dahurica

[0086] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0087] A: degreasing and decolorization

[0088] Take the root of Baizhi root, wash it, dry it at 45°C, and crush it through a 40-mesh sieve. Weigh the powder sample of Angelica dahurica, add petroleum ether at 55°C to reflux for 4 hours, dry at 45°C, then add 80% ethanol to extract at 80°C for 4 hours to remove part of the pigment and oligosaccharides, evaporate the ethanol to dry, pulverize, and dry at 45°C Dry and set aside. The consumption of described sherwood oil is calculated as 3mL by every gram of Chuan Baizhi powder, and the consumption of 80% ethanol is calculated as 2mL by every gram of Chuan Baizhi powder.

[0089] B: extract

[0090] The powder after degreasing and depigmentation was extracted with water at 81°C for 3 times, each time for 1 hour, the ratio of water to Baizhi powder was 68...

Embodiment 3

[0104] Preparation of Polysaccharides from Radix Angelica dahurica

[0105] Compared with Example 2 in this example, the drying temperature in step A is 50°C, and 4 times the amount of petroleum ether of Angelica dahurica is added to heat and reflux for degreasing;

[0106] In step C, slowly add absolute ethanol to the concentrated solution until the final concentration of ethanol in the mixed solution is 70%, place it at 6°C for 24 hours, centrifuge to obtain the precipitation of the polysaccharide of Angelica dahurica dahurica, and discard the supernatant. Redissolve the polysaccharide precipitate of Baizhi Baizhi with water, centrifuge at 4000r / min for 10min to remove impurities, and add ethanol to the supernatant to reach a concentration of 70%. After standing at 6°C for 24 hours, centrifuge for the third time, and take the precipitate after the third centrifugation for future use;

[0107] All the other conditions are with embodiment 2.

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Abstract

The invention discloses an angelica anomala polysaccharide extracted on the basis of response surface optimization and a preparation method and an application thereof. The molecular weight of the angelica anomala polysaccharide is 1.17kDa; after angelica anomala as a medicinal material undergoes water extraction, alcohol precipitation, deproteinization and desalination, chromatographic separation is then carried out by a DEAE-agarose gel FF column and an agarose gel 6FF gel column, and thereby the angelica anomala polysaccharide is obtained; and the conditions of water extraction are as follows: extraction is carried out under 81 DEG C for three times, extraction is carried out for one hour each time, and the dosage ratio of water to angelica anomala powder is 68mL/g. The angelica anomala polysaccharide prepared by the invention has good bacteriostatic activity, oxidation resistance and whitening activity. The method disclosed by the invention has the advantages of high extraction rate and good purification effect, and does not change the structure of the angelica anomala polysaccharide and affect the bioactivity of the angelica anomala polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to polysaccharides from Radix Angelica dahuricae Radix Chuanbai for optimal extraction based on response surface methodology, a preparation method and application thereof. Background technique [0002] Angelica dahurica (Fisch.ex Hoffm) Benth.etHook.f. or Hangbaizhi Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook.f.var.formosana (Boiss) Shan et Yuan Dried roots. Angelica dahurica is pungent in taste, warm in nature, and returns to the stomach, large intestine, and lung meridians. It has the effects of relieving exterior cold, expelling wind and relieving pain, dredging nasal orifices, drying dampness and stopping belt, reducing swelling and discharging pus, and is used for cold headache, brow bone pain, nasal congestion, runny nose, nasal congestion, nasal sinusitis, toothache, belt Next, the sores are swollen and painful. [0003] Angelica dahurica is one of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A01P1/00A61P31/04A61P39/06
Inventor 吴卫唐小琴侯凯潘峰陈艾萌汪丽霞李海馨李石
Owner SICHUAN AGRI UNIV
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