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Efficient preparation and purification method of natural free astaxanthin

A purification method and free state technology, applied in the field of astaxanthin, can solve the problems of large amount of alkali, easy generation of by-products, environmental pollution, etc., and achieve the effects of low cost, simple and easy preparation method and broad market prospect.

Active Publication Date: 2021-04-09
RIZHAO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the traditional saponification method uses a large amount of alkali, causes environmental pollution, and easily produces by-products, the present invention provides a method for preparing and purifying natural free astaxanthin. The method is simple and easy to operate, low in cost, mild in conditions, and environmentally friendly. The content of free astaxanthin in the final product is more than 60%, which can effectively avoid the formation of by-products in the process of enzymatic hydrolysis and enrichment of astaxanthin

Method used

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  • Efficient preparation and purification method of natural free astaxanthin

Examples

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Effect test

Embodiment 1

[0035] A method for preparing and purifying natural free astaxanthin, comprising the steps of:

[0036] (1) Raw material and pretreatment: take Haematococcus pluvialis oil as raw material, add dehydrated alcohol wherein, the addition amount of dehydrated alcohol is 5% of Haematococcus pluvialls oil quality, fully stir and mix uniformly, obtain rain Haematococcus oil pretreatment solution;

[0037] (2) Construction of Haematococcus pluvialis enzymatic hydrolysis system: lipase powder and tea polyphenols are dispersed in deionized water, and then added to the Haematococcus pluvialis pretreatment solution obtained in step (1) to obtain Pluvialls red Algal oil enzymatic hydrolysis system;

[0038] The amount of lipase powder added to the mass fraction of the total enzymolysis system was 1.5%, the amount of antioxidants added to the mass fraction of the total enzymolysis system was 0.01%, and the amount of deionized water accounted for 35% of the mass fraction of the total enzymol...

Embodiment 2

[0046] A method for preparing and purifying natural free astaxanthin, comprising the steps of:

[0047] (1) Raw material and pretreatment: take Haematococcus pluvialis oil as raw material, add dehydrated alcohol wherein, the addition amount of dehydrated alcohol is 25% of Haematococcus pluvialls oil quality, fully stir and mix, obtain rain Haematococcus oil pretreatment solution;

[0048] (2) Construction of Haematococcus pluvialis enzymatic hydrolysis system: disperse lipase powder, sodium edetate and isoascorbic acid in a phosphate buffered saline solution of pH 6.8, and then add it to the pluvialis obtained in step (1). Haematococcus pluvialis oil enzymatic hydrolysis system is obtained in the Haematococcus pretreatment solution;

[0049] The amount of lipase powder added accounted for 4% of the mass fraction of the total enzymolysis system, the amount of antioxidant added accounted for 0.8% of the mass fraction of the total enzymolysis system, and the amount of buffer sol...

Embodiment 3

[0057] A method for preparing and purifying natural free astaxanthin, comprising the steps of:

[0058] (1) Raw material and pretreatment: take Haematococcus pluvialis oil as raw material, add dehydrated alcohol wherein, the addition amount of dehydrated alcohol is 15% of Haematococcus pluvialls oil quality, fully stirs and mixes, obtains rain Haematococcus oil pretreatment solution;

[0059] (2) Construction of Haematococcus pluvialis enzymatic hydrolysis system: lipase powder and erythorbic acid are dispersed in the phosphate buffer solution of pH6.8, then added to the Haematococcus pluvialis pretreatment solution obtained in step (1) , to obtain the Haematococcus pluvialis oil enzymatic hydrolysis system;

[0060] The amount of lipase powder added is 3% in the mass fraction of the total enzymolysis system, the amount of antioxidant added in the mass fraction of the total enzymolysis system is 0.5%, and the amount of buffer solution in the mass fraction of the total enzymol...

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Abstract

The invention relates to the technical field of astaxanthin, in particular to an efficient preparation and purification method of natural free astaxanthin. The preparation and purification method comprises the following steps of (1) performing raw material selection and pretreatment; (2) constructing a haematococcus pluvialis enzymolysis system: dispersing lipase and an antioxidant into deionized water and / or a buffer solution, and then adding the deionized water and / or the buffer solution system containing the lipase and the antioxidant into a haematococcus pluvialis pretreatment solution obtained in the step (1) to obtain a haematococcus pluvialis oil enzymolysis system; (3) performing primary enzymolysis; (4) performing primary enrichment; (5) performing secondary enzymolysis; and (6) performing secondary enrichment. The method is simple and easy to operate, low in cost, mild in condition and environmentally friendly; the content of the free astaxanthin in a finally prepared product reaches 60% or above; and the generation of byproducts in the enzymolysis and enrichment processes of the astaxanthin can be effectively avoided.

Description

technical field [0001] The invention relates to the technical field of astaxanthin, in particular to a high-efficiency preparation and purification method of natural free astaxanthin. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4-diketo-β,β'-carotene) is a red ketone carotenoid found in some aquatic animals. According to reports, the antioxidant activity of astaxanthin is more than 10 times higher than that of β-carotene, and more than 500 times higher than that of vitamin E. It is called "super vitamin E". Astaxanthin can be used as a coloring agent in precious aquatic products and poultry farming to increase its additional commodity value. In addition, the human body can also selectively accumulate astaxanthin, which has anti-oxidation, anti-aging, anti-tumor, and prevention of cardiovascular and cerebrovascular diseases, etc. effect. Therefore, it has received extensive attention in recent years. [0003] In nature, astaxanthin mainly exists in the form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C07C403/24
CPCC12P23/00C07C403/24C07C2601/16
Inventor 周庆新陈芳甜袁溢旋郭楚璇韩鑫杰王岳琦
Owner RIZHAO POLYTECHNIC
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