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Isolated culture method of wild phellinus igniarius mushroom mycopremna

A technology for separating and culturing Phellinus linteus mushrooms, which can be applied to fungi and other directions, can solve the problems of low fermentation yield of mycelium, and achieve the effects of reasonable formula and ratio, reasonable culture and separation method, and promoting the growth and development of mycelium.

Inactive Publication Date: 2008-02-27
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the problem of low mycelium fermentation yield in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The isolation and cultivation method of Chinese wild phellodendron phellodendron strain, comprises the following steps:

[0018] (1) Production of culture medium: make test tube slant culture medium according to the following formula and proportioning

[0019] The formula of the medium: potato 250g, glucose 20g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, vitamin B 1 10mg, mulberry branches 85g, agar 20g, water 1000mL, pH6.5;

[0020] The preparation method of the culture medium is as follows: first cut the mulberry branches into small sections of 1 to 2 cm, add 1500 mL of water to boil for 30 minutes, and filter to get the juice. Then peel the potatoes, cut them into 1cm thick slices, add them to the juice and boil for 10 minutes, filter to get the filtrate. Add glucose, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B to this filtrate 1 , boil over low heat until the agar dissolves, and dilute to 1000mL. Pack the prepared medium i...

Embodiment 2

[0027] The isolation and cultivation method of Chinese wild phellodendron phellodendron strain, comprises the following steps:

[0028] (1) Production of culture medium: make test tube slant culture medium according to the following formula and proportioning

[0029] The formula of the medium: potato 220g, glucose 25g, potassium dihydrogen phosphate 0.8g, magnesium sulfate 0.6g, vitamin B 1 8mg, mulberry branches 100g, agar 18g, water 1000mL, pH6.0;

[0030] The preparation method of the culture medium is as follows: first cut the mulberry branches into small sections of 1 to 2 cm, add 1800 mL of water to boil for 30 minutes, and filter to get the juice. Then peel the potatoes, cut them into 1cm thick slices, add them to the juice and boil for 10 minutes, filter to get the filtrate. Add glucose, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B to this filtrate according to the ratio 1 , boil over low heat until the agar dissolves, and dilute to 1000mL. P...

Embodiment 3

[0036] The isolation and cultivation method of Chinese wild phellodendron phellodendron strain, comprises the following steps:

[0037] (1) Production of culture medium: make test tube slant culture medium according to the following formula and proportioning

[0038] The formula of the medium: potato 260g, glucose 18g, potassium dihydrogen phosphate 1.2g, magnesium sulfate 0.4g, vitamin B 112mg, mulberry branches 80g, agar 20g, water 1000mL, pH7.0;

[0039] The preparation method of the culture medium is as follows: first cut the mulberry branches into small sections of 1 to 2 cm, add 1200 mL of water to boil for 30 minutes, and filter to get the juice. Then peel the potatoes, cut them into 1cm thick slices, add them to the juice and boil for 20 minutes, then filter to get the filtrate. Add glucose, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B to this filtrate according to the ratio 1 , boil over low heat until the agar dissolves, and dilute to 1000mL....

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Abstract

The invention discloses a separating culturing method of Chinese wild morin mushroom strain in the bacterial separating culture technical domain of fungi, which comprises the following steps: (1) making the culture medium of test tube oblique plane according to the proportion and dispense with 220-260g potato, 18-25g glucose, 0. 8-1. 2g potassium biphosphate, 0. 4-0. 6g magnesium sulfate, 18-12mg vitamin B, 80-100g mulberry branch, 18-20g agar, 1000mL water with pH value at 6-7. 0; (2) separating to culture: disposing the solid of morin; separating morin; culturing mother seed. The invention can accelerate the growth and breeding of hypha effectively to improve the activity of the strain, which realizes the high production of hypha fermented by morin liquor to reach 8. 83g dried hypha in the 1000ml liquor evenly.

Description

Technical field: [0001] The invention relates to the technical field of strain separation and cultivation of medicinal fungi, in particular to a method for separation and cultivation of Chinese wild Phellinus phellidis strains. Background technique: [0002] Phellinus belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenochaeyaceae, Phellinus, grows in poplar , mulberry, willow, birch, beech, peach and other broad-leaved trees, standing trees and trunks are P.igniarius, P.baumii (P.linteus) common name. Sanghuang looks like a piece of loess in the early stage, and after a period of growth, it looks like a tongue sticking out of a tree stump. Fruiting bodies are perennial, laterally sessile, hard woody, hoof-shaped, oblate hemispherical or irregular in shape, cover surface light liver brown to taupe or black, with fine hairs at the beginning, often with obvious vertical and horizontal cracks when old, with Concentric ring ribs, blunt edges, yellow flanging, egg...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 雷萍孙悦迎吴亚召张文隽张慧
Owner MICROBIOLOGY INST OF SHAANXI
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