Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same

A technology of Phellinus linteus mycelium and culture medium, which is applied in the biological field, can solve the problems of plundering, depletion, and inability to find Phellinus linteus resources by medicinal farmers, and achieves the effects of clear composition, stable quality and rapid growth.

Inactive Publication Date: 2011-03-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the increasing demand for Phellinus Phellinus in domestic and foreign markets, drug farmers in various domestic production areas have predatory collections, and fruiting body spores cannot be formed in large quantities. As a result, Phellinus Phellinus resources are hard to find in my country and will soon be exhausted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Configuration Phellinus mycelium culture medium 1L described in the present invention, each component weight in every liter is: maltose 28g, yeast powder 22g, MgSO 4 1.2g, KH 2 PO 4 0.9g, triammonium citrate 1.1g, vitamin B18mg, vitamin B25mg, vitamin B60.6mg, nicotinamide 28mg, calcium pantothenate 3.0mg, water extract of Mori twig 5g. Divide the culture medium into 50mL shake flasks, the filling volume is 25mL, the medium sterilization temperature is 120°C, and the sterilization time is 30min.

[0017] The technique of using the substratum to ferment Phellinus mycelia: put the Phellinus mycelium into the nutrient solution and cultivate it for 4 days, the inoculum size is 13% (V / V), the culture temperature is 25° C., the shake bottle liquid volume is 50%, The rotating speed of the reciprocating shaker is 110rpm, and the fermentation time is 9 days. Samples were taken every 24 hours, centrifuged at 8000 rpm for 10 minutes, mycelia were washed 3 times with distilled...

Embodiment 2

[0019] Configure Phellinus mycelium culture medium 1L described in the present invention, each component weight in every liter is: maltose 30g, yeast powder 20g, MgSO 4 1.0g, KH 2 PO 4 1.0g, triammonium citrate 1.0g, vitamin B1 8mg, vitamin B24mg, vitamin B60.52mg, nicotinamide 26mg, calcium pantothenate 2.6mg, water extract of Mori twig 5g. Divide the culture medium into 50mL shake flasks, the filling volume is 30mL, the culture medium sterilization temperature: 121°C, sterilization time: 30min.

[0020] The process of using the substratum to ferment Phellinus mycelia: put Phellinus mycelia into culture solution and cultivate for 5 days, inoculum size 15% (V / V), culture temperature 27°C, shake bottle liquid volume 60%, The rotating speed of the reciprocating shaker is 120rpm, and the fermentation time is 7 days. Samples were taken every 24 hours, centrifuged at 8000rpm for 10 minutes, washed with distilled water for 3 times, vacuum freeze-dried at -50°C until constant we...

Embodiment 3

[0022] Configuration Phellinus mycelium culture medium 1L described in the present invention, each component weight in every liter is: maltose 35g, yeast powder 22g, MgSO 4 1.1g, KH 2 PO 4 1.2g, triammonium citrate 1.1g, vitamin B17mg, vitamin B26mg, vitamin B60.6mg, nicotinamide 28mg, calcium pantothenate 3.0mg, water extract of mulberry branch 4g. Divide the culture medium into 50mL shake flasks, the filling volume is 35mL, the culture medium sterilization temperature: 122°C, sterilization time: 30min.

[0023] The process of using the culture medium to ferment Phellinus mycelia: put Phellinus mycelium into the culture solution and cultivate for 4 days, the inoculum size is 14% (V / V), the culture temperature is 28°C, the liquid volume of the shaking bottle is 70%, The rotating speed of the reciprocating shaker is 120rpm, and the fermentation time is 7 days. Samples were taken every 24 hours, centrifuged at 8,000 rpm for 10 minutes, washed with distilled water for 3 time...

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Abstract

The invention discloses a phellinus igniarius mycelium culture medium and a process for fermenting phellinus igniarius using the same. The phellinus igniarius culture medium comprises the following components per liter: 25-35 g of maltose, 18-25 g of yeast powder, 2.4-4.2 g of mineral elements, 5-10 mg of vitamin B1, 3-5 mg of vitamin B2, 0.5-1 mg of vitamin B6, 20-30 mg of niacinamide, 2-3 mg of calcium pantothenate, 3-10 g of aqueous extract of ramulus mori and the balance of water. The phellinus igniarius is cultured in a culture solution for 4-6 days, wherein the inoculation amount is 10-20%, the culture temperature is 22-29 DEG C, the liquid amount in a shake flask is 50-70%, the rotation speed of a reciprocating shaker is 100-150 rpm and the fermentation time is 5-10 days. The invention has the advantages that: the components are clear, the quality is stable, a special nutrition source for phellinus igniarius is provided, and the invention can be used for the large-scale culture of phellinus igniarius mycelium with medicinal activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Phellinus mycelia culture medium and a process for fermenting Phellinus mycelium with the culture medium. Background technique [0002] Phellinus, Basidiomycotina, Phyllomycetes, Polyporacea, Polyporaceae, Pinfoliobacteria fungi. Its scientific name is Phellinus Linteus, and its Chinese name is Acupunculus clefthoof. attention of the healthcare industry. In recent years, Japan and South Korea have successively developed it, and it is mainly used in the treatment of hepatitis and cancer, with remarkable curative effect. According to the relevant data, Phellinus has a tumor inhibition rate as high as 96.78%, and is currently internationally recognized as the most effective medicinal fungus in the field of biological anti-cancer. [0003] In my country, Phellinus Phellinus has been used as medicine for more than 2,000 years, and the earliest records began in "Compendium of Materia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
Inventor 计东风钟石李有贵朱俭勋吕志强林天宝
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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