Anoectochilus roxburghiiv flavone extraction process and application thereof in skin care products

An extraction process and technology of lotus flavonoids are applied in the field of extraction process of flavonoids, which can solve the problems of low content of active components, a large amount of impurities, and low extraction rate, and achieve the effects of high content of active components, good antioxidant activity and few impurities.

Active Publication Date: 2017-02-22
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The primary separation and purification method of macroporous resin takes a long time and the extraction rate is low, while the solvent extraction method requires a large amount of solvent and the content of impurities in the extract is high; in addition, the effective lower content of ingredients

Method used

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  • Anoectochilus roxburghiiv flavone extraction process and application thereof in skin care products
  • Anoectochilus roxburghiiv flavone extraction process and application thereof in skin care products
  • Anoectochilus roxburghiiv flavone extraction process and application thereof in skin care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Fresh clematis was beaten, freeze-dried and pulverized at freezing temperature -78°C, freezing time 48h, and vacuum pressure 20Pa to obtain clematis powder; weigh 3.00g of clematis powder, and in ultrasonic power of 600W, The ratio of solid to liquid is 1:150, the concentration of ethanol is 60%, the extraction time is 60 minutes, and the extraction temperature is 60°C, and the crude extract of flavonoids of Auranthus clematis is obtained;

[0034] (2) Filter the crude extract of Aureus flavonoids under the conditions of -0.1MPa vacuum, double-layer filter paper with a pore size of 15-20um, and then filter under the conditions of -0.1MPa vacuum, rotation speed 115rpm / min, evaporation temperature 45°C, and cycle condensation temperature Concentrating under reduced pressure at 2°C to obtain the crude extract of flavonoids from Auranthus clematis;

[0035] (3) Pass the crude extract of clematis flavonoids over Sephadex LH-20 gel column, the ratio of the column diameter...

Embodiment 2

[0037] (1) Fresh clematis was beaten, freeze-dried and pulverized at freezing temperature -70°C, freezing time 40h, and vacuum pressure 15Pa to obtain clematis powder; weigh 3.00g of clematis powder, microwave power 800W, The ratio of solid to liquid is 1:100, the concentration of ethanol is 50%, the extraction time is 40 minutes, and the extraction temperature is 40°C, so as to obtain the crude extract of the flavonoids of Auranthus chinensis;

[0038](2) Filter the crude extract of Aureus flavonoids under the conditions of -0.1MPa vacuum, double-layer filter paper with a pore size of 15-20um, and then filter under the conditions of -0.1MPa vacuum, rotation speed 115rpm / min, evaporation temperature 45°C, and cycle condensation temperature Concentrating under reduced pressure at 2°C to obtain the crude extract of flavonoids from Auranthus clematis;

[0039] (3) Pass the crude extract of clematis flavonoids over Sephadex LH-20 gel column, the ratio of the column diameter of the...

Embodiment 3

[0041] (1) Fresh clematis was beaten, freeze-dried and pulverized at freezing temperature -80°C, freezing time 60h, and vacuum pressure 25Pa to obtain clematis powder; weigh 3.00g clematis powder, and microwave at 400W, The ratio of solid to liquid is 1:200, the concentration of ethanol is 60%, the extraction time is 1200min, and the extraction temperature is 80°C, and the crude extract of flavonoids of Auranthus clematis is obtained;

[0042] (2) Filter the crude extract of Aureus flavonoids under the conditions of -0.1MPa vacuum, double-layer filter paper with a pore size of 15-20um, and then filter under the conditions of -0.1MPa vacuum, rotation speed 115rpm / min, evaporation temperature 45°C, and cycle condensation temperature Concentrating under reduced pressure under the condition of 2°C to obtain the crude extract of Aurantia chinensis flavonoids;

[0043] (3) Pass the crude extract of clematis flavonoids over Sephadex LH-20 gel column, the ratio of the column diameter ...

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Abstract

The invention relates to an anoectochilus roxburghii flavone extraction process and application thereof in skin care products. The extraction process comprises the following steps of S1, conducting pulping, freeze drying and smashing on the anoectochilus roxburghii in sequence, and conducting extraction on the smashed anoectochilus roxburghii powder to obtain an anoectochilus roxburghii flavone crude extract liquid; S2, filtering the crude extract liquid obtained in the S1, reducing pressure and concentrating to obtain an anoectochilus roxburghii flavone crude extract; S3, conducting purification on the anoectochilus roxburghii flavone crude extract by adopting a Sephadex LH-20 gel column, so that an anoectochilus roxburghii flavone extract is obtained, wherein technological conditions in the S1 for freeze drying is a freezing temperature of 70-80 DEG C, freezing time of 40-60 hours and vacuum pressure of 15-25Pa. The provided extraction technology has high an extraction rate, and the anoectochilus roxburghii flavone extract obtained by extraction has high content of effective constituents, less impurities, and good antioxidant activity and tyrosinase activity inhibition.

Description

technical field [0001] The invention relates to an extraction process of flavonoids, in particular to an extraction process of aureus flavonoids and its application in skin care products. Background technique [0002] Anoecwchilus roxburghii (Wall.) Lindl] is a plant of the Orchidaceae roxburghii genus, which is a perennial rare Chinese herbal medicine. Golden silkworm, golden stone pine, tree grass lotus, money seed grass, etc. The whole herb of Clematis clematis can be used as medicine. It is sweet in taste and flat in nature. It has the effects of clearing heat and cooling blood, dehumidification and detoxification, pain relief, and antitussive. Hepatitis, nephritis, cystitis and other diseases; folks are used to treat acute nephritis, convulsions in children, and wind-cold-damp arthralgia, etc., and the curative effect is particularly significant. It enjoys the reputation of "King of Medicine", "Golden Grass", and "Black Ginseng". The main active ingredients of clemati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61K8/49A61Q19/00A61Q19/02
CPCA61K8/498A61K8/97A61K2800/782A61K2800/82A61K2800/84A61Q19/00A61Q19/02
Inventor 刘露孙连立聂艳峰裴运林林宇祺张黎兴
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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