Method for extracting polygahatous polysaccharides from traditional Chinese medicine rhizoma polygonati
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A Polygonatum Polysaccharide and Rhizoma Polygonatum technology, which is applied in the field of extraction of traditional Chinese medicine components, can solve the problems of low product yield, unsatisfactory, low purity, etc., and achieve the effects of reducing extraction time, high production efficiency, and improving extraction rate
Inactive Publication Date: 2014-05-14
XUANCHENG BAICAO PLANT IND & TRADE CO LTD
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Embodiment 1
[0014] (1) Take 150g of Rhizoma Polygonatum, wash it, ventilate and dry it to remove the moisture in Raw Rhizoma Rhizoma Polygonatum, make its moisture content lower than 2%, and then make it into 600-mesh Rhizoma Rhizoma Rhizoma Polygonatum powder with a grinder;
[0015] (2) Use 70% ethanol as a solvent to carry out secondary microwave-assisted extraction of the Polygonatum powder prepared above. The microwave power of each extraction is 600W, the ratio of solid to liquid is 1:10, and the extraction time is 25min each time. Filter, Combine the secondary extracts;
[0016] (3) After recovering hexyl alcohol from the above combined extracts, concentrate to a relative density of 1.12, then add 4 times the amount of 95% ethanol, stir evenly, and then place it in an ultrasonic oscillator for ultrasonic oscillation for 1 hour, with an ultrasonic frequency of 35KHz , the ultrasonic power is 700W, let stand for 24h, centrifuge and filter to obtain sediment, and dry to obtain 20.3...
Embodiment 2
[0019] (1) Take 150g of Rhizoma Polygonatum, wash it, ventilate and dry it to remove the moisture in Raw Rhizoma Rhizoma Polygonatum, make its moisture content lower than 2%, and then make it into 800 mesh Rhizoma Rhizoma Rhizoma Polygonatum powder with a grinder;
[0020] (2) Use 80% hexanol as the solvent to carry out secondary microwave-assisted extraction of the above-mentioned Polygonatum powder. The microwave power of each extraction is 700W, the ratio of solid to liquid is 1:15, and the extraction time is 30min each time. Filter, Combine the secondary extracts;
[0021] (3) After recovering the hexyl alcohol from the above combined extracts, concentrate to a relative density of 1.14, then add 5 times the amount of 90% ethanol, stir evenly, and then place it in an ultrasonic oscillator for 0.5h ultrasonic oscillation, the ultrasonic frequency 40KHz, the ultrasonic power is 800W, let stand for 24h, centrifuge to filter the precipitate, and dry to obtain 21.11g of crude...
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Abstract
The invention discloses a method for extracting polygahatous polysaccharides from traditional Chinese medicine rhizoma polygonati. The method comprises the steps of cleaning, drying and grinding the rhizoma polygonati; performing secondary microwave auxiliary extraction on rhizoma polygonati powder through hexanol serving as a solvent, filtering and combining secondary extraction liquid; recycling the hexanol from the combined extraction liquid, then concentrating the extraction liquid, adding ethyl alcohol, performing uniform stirring, putting into an ultrasonic oscillator for ultrasonic oscillation, standing, performing centrifugal filtration to obtain precipitates, and drying the precipitates to obtain coarse polygahatous polysaccharides; removing proteins from the coarse polygahatous polysaccharides through an ethanol solution, recycling ethanol, concentrating to concentrated paste, dissolving the concentrated paste into a Tris-HCl buffering solution, and purifying the polygahatous polysaccharides twice through DE-52 cellulose column chromatography; performing third-time purification through distilled water by SephadexG-100 sephadex gel filtration chromatography, concentrating and carrying out freeze drying to obtain a refined polygahatous polysaccharides product. The extraction method disclosed by the invention is simple and feasible; the production efficiency is high; the quality of the prepared product is high; the yield is over 90 percent, and the purity is over 99 percent; the method is suitable for industrial large-scale production.
Description
technical field [0001] The invention relates to a method for extracting Polygonatum Polysaccharide from Chinese medicine Rhizoma Polygonatum, and belongs to the technical field of extraction of traditional Chinese medicine components. Background technique [0002] Polygonatum Polygonatum is the dried rhizome of Polygonatum Polygonatum, Polygonatum Polyflora or Polygonatum Polyflora of Liliaceae. It is a traditional Chinese medicine and belongs to the homology of medicine and food. Modern pharmacological research results show that Polygonatum polysaccharide is the main active ingredient, which has physiological functions such as anti-tumor, regulating blood sugar and blood lipids, anti-aging, and anti-oxidation. In addition, Polygonatum polysaccharide also has anti-virus and immune activation effects. With the continuous deepening of people's understanding of the functions of Polygonatum polysaccharides, the market demand for the extraction and separation technology of Polygo...
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