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Method for extracting solanesol, cembrane diterpene, vitamin E and phytosterol from tobaccos simultaneously

A technology of cembrane diterpene and phytosterol, which is applied in the field of extracting vitamin E, cembrane diterpene, phytosterol, and solanesol at the same time, can solve the problems of waste of fat-soluble active ingredients, so as to improve the utilization value, realize utilization, The effect of improving utilization efficiency

Active Publication Date: 2016-10-12
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problem that other fat-soluble active ingredients are discarded in the extraction process of tobacco solanesol, the object of the present invention is to provide a method for simultaneously extracting solanesol, cembrane diterpenes, vitamin E, and phytosterols from tobacco. The remaining tobacco residue after extraction with fat-soluble organic solvents can also be used for the extraction of tobacco protein, chlorogenic acid, rutin, polysaccharides, and nicotine

Method used

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  • Method for extracting solanesol, cembrane diterpene, vitamin E and phytosterol from tobaccos simultaneously

Examples

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Effect test

Embodiment 1

[0030] Such as figure 1 Shown, a kind of method for simultaneously extracting solanesol, cembrane diterpene, vitamin E, phytosterol from tobacco, specifically comprises the steps:

[0031] (1) Extraction: Take the prepared low-grade flue-cured tobacco leaves, dry them at 60°C, crush them, weigh 200g of the crushed tobacco powder, use n-hexane as the extractant, the ratio of solid to liquid is 1:10, ultrasonically extract at 45°C for 30min, centrifuge Or filter, and the residue is washed twice with the extract (the ratio of solid to liquid twice is 1:8, 1:6 respectively), the extract is combined, concentrated under reduced pressure and the solvent is recovered to obtain 12.5g extract;

[0032] (2) Saponification: Add 120mL of 95% ethanol and 2g of sodium hydroxide to the extract obtained in the above steps, and heat and reflux saponification at 60°C for 1h. After the saponification is completed, adjust the pH value to neutral and concentrate to remove the organic solvent. Add ...

Embodiment 2

[0038] Such as figure 1 Shown, a kind of method for simultaneously extracting solanesol, cembrane diterpene, vitamin E, phytosterol from tobacco, specifically comprises the steps:

[0039] (1) Extraction: take the unsuitable lower or upper part of flue-cured tobacco in the mature stage of the field, dry it at 60° C., grind it, weigh 200 g of the ground tobacco powder, use petroleum ether as the extractant, and the remaining steps are the same as in Example 1;

[0040] (2) Saponification: the used extraction solvent for extracting the fat-soluble active ingredient after saponification is completed in this saponification process is sherwood oil, and all the other steps are with implementation example 1.

[0041] Following separation steps are the same as implementation example 1.

Embodiment 3

[0043] Such as figure 1 Shown, a kind of method for simultaneously extracting solanesol, cembrane diterpene, vitamin E, phytosterol from tobacco, specifically comprises the steps:

[0044] (1) Extraction: take mature sun-cured tobacco leaves, dry at 60° C., pulverize, weigh 200 g of pulverized tobacco dust, and use petroleum ether as the extractant. The following steps are the same as in Example 1.

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Abstract

The invention discloses a method for extracting solanesol, cembrane diterpene, vitamin E and phytosterol from tobaccos simultaneously. The method comprises the steps that 1, extraction is carried out; 2, saponification is carried out, wherein saponified solanesol and other fat-soluble active ingredient extracts are obtained; 3, sephadex gel column separation is carried out, wherein solanesol, vitamin E and phytosterol (mixture) and cembrane diterpene crude extract are obtained respectively; 4, silica-gel column chromatography is carried out, wherein after silica-gel column chromatography, high-purity solanesol, vitamin E and phytosterol are obtained respectively; the obtained cembrane diterpene is subjected to silica-gel column chromatography, and alpha-4,8,13-cembratriene-1,3-diol and beta-4,8,13-cembratriene-1,3-diol are obtained respectively. Comprehensive extraction of tobacco fat-soluble active ingredients is achieved, tobacco residue obtained after fat-soluble active ingredients are extracted can be used for extraction of tobacco protein, chlorogenic acid, rutin, polysaccharide and nicotine, gradient utilization of tobacco active ingredients is achieved, and the utilization value of tobacco resources, particularly waste tobaccos is improved.

Description

technical field [0001] The invention relates to the technical field of comprehensively extracting fat-soluble active substances from tobacco, in particular to a method for simultaneously extracting solanesol, cembrane diterpene, vitamin E and phytosterol from tobacco. Background technique [0002] my country is the world's largest producer of tobacco leaves, with an annual planting area of ​​1.3-1.5 million hectares and an annual output of 2-3 million tons of tobacco leaves. On the one hand, in the field production of tobacco leaves, about 1 / 4 of fresh tobacco leaves (including the lower , upper part) are discarded as unsuitable tobacco leaves. On the other hand, among the tobacco leaves purchased by cigarette companies, about 1 / 4 of low-grade tobacco leaves, leftovers, tobacco powder, etc. cannot be used as raw materials for cigarettes, resulting in a huge waste of resources. waste. [0003] In order to make full use of waste tobacco resources, more and more attention has be...

Claims

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Application Information

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IPC IPC(8): C07D311/72C07J9/00C07C29/74C07C29/86C07C29/88C07C29/76C07C33/02C11B9/00A24B3/12
CPCA24B3/12C07C29/74C07C29/76C07C29/86C07C29/88C07D311/72C07J9/00C11B9/022C11B9/025C07C33/02
Inventor 杜咏梅刘新民顔培真张忠锋王爱华付秋娟张鹏谭家能
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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