Separation purification method of catechin monomer

A technology for separation and purification of catechins, applied in the field of separation and purification of catechin monomers, can solve the problems of long elution time, high price, high separation cost, etc., achieve reduced production costs, fast and convenient recovery, and shorten the connection process Effect

Inactive Publication Date: 2005-04-06
HEFEI UNIV OF TECH
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Problems solved by technology

For the isomer GCG with EGCG, the current report can only be separated by preparative HPLC
In addition, the eluent used in the dextran gel chromatography method also has the disadvantages of long elution time and easy oxidation of the product.
[0021] (2) High separation cost
Due to the high price of preparative HPLC chromatographic column, and it is easy to be polluted and blocked, the service life is short, the production cost is high, and the one-time sample volume i

Method used

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  • Separation purification method of catechin monomer

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Experimental program
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Embodiment

[0045] see figure 1 , according to the process route as follows,

[0046] (1) First extract the crude tea polyphenols (TP) containing catechins from tea powder;

[0047] (2) Pre-separation of catechin monomer L-epigallocatechin gallate EGCG and L-epicatechin gallate ECG:

[0048] a. Pretreatment of the gel column: take 70g Sephadex LH-20 xerogel by weighing, fully swell with 300ml eluent absolute ethanol (can be heated in a boiling water bath for 2 hours to swell to shorten the swelling time);

[0049] b. Column packing: Slowly pour the swollen gel into the chromatography column to make it settle evenly. After settling to the desired height of about 50cm, continue to rinse with 3-5 times the column bed volume of absolute ethanol to balance the column;

[0050] c. Sample loading: Weigh 5.8140 grams of raw tea polyphenols and dissolve them in 20ml of absolute ethanol to fully dissolve them. After gluing, connect the automatic part collector to start collecting. The elution f...

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Abstract

This invention relates to separation and purification method of catechin monomer EGCG and ECG. The features are that dextrane gel Sephadex LH-20 is column filling and eluant is absolute ethyl alcohol. Then column chromatography is made by dextrane gel Sephadex LH-20 and 40% ethanol aqueous solution be eluant, that is chromatography column non- gradient expendable separation is adopted. Comparing to original method, equipments are facilitated greatly and the method is simple, cost is low. The solvent is nontoxic and separation period is short, and monomer extraction rate and product purity are both high.

Description

technical field [0001] The invention relates to a separation and purification method of two important catechin monomers L-epicatechin gallate EGCG and L-epicatechin gallate ECG in tea leaves. Background technique [0002] Catechin is an important class of natural active substances in tea, which belongs to flavanol compounds. It was first extracted and isolated by the Japanese in 1929. It consists of about eight monomers, namely D, L-catechin (D, L-C), L-epicatechin (L-EC), L-epigallocatechin (L-EGC), D , L-gallocatechin (D, L-GC), L-epicatechin gallate (L-ECG), L-catechin ester (L-CG), L-epigallocatechin Gallate (L-EGCG), L-gallocatechin gallate (L-GCG). Because of the esterification of catechin and gallic acid in CG, ECG, GCG, and EGCG, they are called ester catechins; while C, EC, GC, and EGC that have not undergone esterification are called non-esterified. Ester catechins, the structure of each catechin is relatively similar, some are isomers, the main difference in st...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 姜绍通黄静潘丽军马道荣陈晓燕
Owner HEFEI UNIV OF TECH
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