Separation purification method of catechin monomer

Abstract

This invention relates to separation and purification method of catechin monomer EGCG and ECG. The features are that dextrane gel Sephadex LH-20 is column filling and eluant is absolute ethyl alcohol. Then column chromatography is made by dextrane gel Sephadex LH-20 and 40% ethanol aqueous solution be eluant, that is chromatography column non- gradient expendable separation is adopted. Comparing to original method, equipments are facilitated greatly and the method is simple, cost is low. The solvent is nontoxic and separation period is short, and monomer extraction rate and product purity are both high.

Description

technical field

[0001] The invention relates to a separation and purification method of two important catechin monomers L-epicatechin gallate EGCG and L-epicatechin gallate ECG in tea leaves. Background technique

[0002] Catechin is an important class of natural active substances in tea, which belongs to flavanol compounds. It was first extracted and isolated by the Japanese in 1929. It consists of about eight monomers, namely D, L-catechin (D, L-C), L-epicatechin (L-EC), L-epigallocatechin (L-EGC), D , L-gallocatechin (D, L-GC), L-epicatechin gallate (L-ECG), L-catechin ester (L-CG), L-epigallocatechin Gallate (L-EGCG), L-gallocatechin gallate (L-GCG). Because of the esterification of catechin and gallic acid in CG, ECG, GCG, and EGCG, they are called ester catechins; while C, EC, GC, and EGC that have not undergone esterification are called non-esterified. Ester catechins, the structure of each catechin is relatively similar, some are isomers, the main difference in st...

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