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Oyster polysaccharide, preparing method and its application in preparing cosmetics

A technology of oyster polysaccharide and cosmetics, applied in oyster polysaccharide and preparation and its application field in the preparation of cosmetics

Active Publication Date: 2007-08-08
源美生物技术开发(大连)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of research on the isolation of polysaccharides from Dalian Bay oyster and Pacific oyster and their moisturizing effects in cosmetics

Method used

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  • Oyster polysaccharide, preparing method and its application in preparing cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Take Dalian Bay oysters or Pacific oysters, and wash the oysters with 0-10°C water;

[0021] b. use a high-pressure container, boil oysters with water at a pressure of 0.3MPa and a temperature of 70°C for 20 minutes, the weight ratio of water to oysters is 1.5 to 3: 1, filter and collect the supernatant;

[0022] c. Centrifuge to remove the precipitate, adjust the supernatant to pH 5 with 60% acetic acid, acidify for 1 hour, and use a membrane separator to intercept substances with a molecular weight below 30,000 Da;

[0023] d. Use NaOH to adjust the pH of the filtrate above 30000Da to 6, centrifuge to remove the precipitate, and take the supernatant to dialyze in distilled water to remove salt;

[0024] e. Pass through a Sephadex G-100 molecular sieve chromatography column to collect OD 280nm Absorption peak, dialysis to desalinate and then freeze-drying to obtain oyster polysaccharide.

[0025] Oyster polysaccharides were detected by enzymatic hydrolysis or enzy...

Embodiment 2

[0027] a. Take Dalian Bay oysters or Pacific oysters, and wash the oysters with 0-10°C water;

[0028] b. Use a high-pressure container to cook oysters with water at a pressure of 0.5 MPa and a temperature of 80° C. for 30 minutes. The weight ratio of water to oysters is 1.5 to 3: 1, filter and collect the supernatant;

[0029] c. Centrifuge to remove the precipitate, adjust the supernatant to pH 6 with 70% acetic acid, acidify for 3 hours, and use a membrane separator to intercept substances with a molecular weight below 30000Da;

[0030] d. Use NaOH to adjust the pH of the filtrate above 30000Da to 8, centrifuge to remove the precipitate, and take the supernatant to dialyze in distilled water to remove salt;

[0031] e. Pass through a Sephadex G-100 molecular sieve chromatography column to collect OD 280nm Absorption peak, dialysis to desalinate and then freeze-drying to obtain oyster polysaccharide.

[0032] Oyster polysaccharides were detected by enzymatic hydrolysis or ...

Embodiment 3

[0034] a. Take Dalian Bay oysters or Pacific oysters, and wash the oysters with 0-10°C water;

[0035] b. Use a high-pressure container to boil oysters at 0.4MPa pressure and 75°C for 45 minutes. The weight ratio of water to oysters is 1.5-3:1, filter and collect the supernatant;

[0036] c. Centrifuge to remove the precipitate, adjust the supernatant to pH 6 with 70% acetic acid, acidify for 2 hours, and use a membrane separator to intercept substances with a molecular weight below 30000Da;

[0037] d. Use NaOH to adjust the pH of the filtrate above 30000Da to 7, centrifuge to remove the precipitate, and take the supernatant to dialyze in distilled water to remove salt;

[0038] e. Pass through a Sephadex G-100 molecular sieve chromatography column to collect OD 280nm Absorption peak, dialysis to desalinate and then freeze-drying to obtain oyster polysaccharide.

[0039] Oyster polysaccharides were detected by enzymatic hydrolysis or enzymatic hydrolysis and nuclear magneti...

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Abstract

The invention discloses an oyster polysaccharide (alpha-1, 4; 1, 6-gluglucosan and beta-1, 6-gluglucosan with relative molecular weight at 4000-6500Da) and making method and application in the cosmetics, which comprises the following steps: washing Dalian bay oyster or Atlantic oyster through water at 0-10 deg.c; boiling oyster at 70-80 deg.c under 0.3-0.5 Mpa for 20-45min; filtering; collecting supernatant; centrifuging to remove sediment; adjusting pH value of supernatant to 5-6 through acetic acid; acidolyzing for 1-3h; intercepting material with molecular weight less than 30000Da through film separator; adjusting pH value of filtrate over 30000Da to 6-8 through NaOH; centrifuging to remove sediment; dialyzing supernatant in the distilled water; passing tomographic column of Sephadex G-100 molecular sieve; collecting material at adsorbing peak with OD at 280 nm; freezing; drying.

Description

Technical field: [0001] The invention relates to an oyster polysaccharide isolated and extracted from Dalian Bay oyster (Crassostrea talienwhanensis Crosse) and Pacific oyster (Crassostrea gigas) for the first time, a preparation method and application in cosmetics. Background technique: [0002] The development of marine biological resources has become a hot spot at present. As a new class of physiologically active substances, marine polysaccharides have great potential in disease diagnosis and prevention, nutrition and health care, animal husbandry, plant growth regulation, and disease resistance. The development of functional polysaccharides has developed internationally. It has become an important industry involving medicine, chemistry, engineering and other disciplines and applied to food, medicine, feed, and agriculture. Dalian Bay oyster is a unique oyster species in my country. There are many studies on the bioactive peptides and specific proteases contained in its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61K8/97A61Q19/00A61K8/9794
Inventor 李伟金桥佟长青赵前程曲敏俞微微谢智芬汪秋宽
Owner 源美生物技术开发(大连)有限公司
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