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Preparation of wood louse powder extract and uses thereof

A technology of extracts and soft-shelled turtles is applied in the application field of preparing analgesic drugs, and can solve problems such as inconvenience of patients

Active Publication Date: 2009-03-25
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a large dose inconveniences the patient

Method used

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  • Preparation of wood louse powder extract and uses thereof
  • Preparation of wood louse powder extract and uses thereof
  • Preparation of wood louse powder extract and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of water-extracted polypeptide coarse powder of soft-shelled turtle

[0049] 15g of dry soil turtle powder (obtained by grinding dry soil turtle) was suspended in 500 ml of distilled water. The resulting suspension was stirred at a constant temperature of 50°C for 60 minutes. The suspension is naturally filtered under normal pressure, and the filtrate is freeze-dried to obtain 3.0 g (20%) of the crude soft-shelled turtle water-extracted polypeptide powder, which is stored at 4°C until use.

Embodiment 2

[0050] Example 2 Separation and Purification of Polypeptide Coarse Powder from Soft-shelled Turtle

[0051] Sephadex G25 is treated and packed into a column (2.6×60cm), 0.007mol / L ammonium acetate is filtered through a 0.45nm microporous membrane and used as a buffer balance. 3.0g of water-extracted polypeptide coarse powder of soft-shelled turtle was dissolved in 0.5ml of distilled water and centrifuged at 10,000 rpm for 10 minutes. The supernatant was applied to Sephadex G25 chromatography column, eluted with 0.007mol / L ammonium acetate buffer, flow rate 0.8mL / min, 8min collected in one tube, each tube was measured by UV spectrophotometer, wavelength 280nm, recorded data, Use Microsoft Excel to make a curve, combine the fractions according to the peak shape, and freeze-dry. Obtained 0.20g (6.7%) W I , 0.50g (17%) W II , 0.99g (33%) W III And 0.30g (10.0%) W IV Four water-extracted peptide components.

Embodiment 3WI

[0052] Example 3W I , W II , W III And W IV Analgesic activity determination

[0053] Divide male ICR mice (22±2g) into a control group (W 0 ), and W I , W II , W III And W IV Treatment group, 10 mice in each group. W I , W II , W III And W IV Dissolve them with 0.3ml of saline. W I , W II , W III And W IV The gavage dose is calculated according to the dose (mg / kg) = 9.01×38mg / piece×yield rate, that is, 0.4mg / 0.3ml / piece W I , 9.1mg / 0.3ml / W II , 12.5mg / 0.3ml / W III With 3.2mg / 0.3ml / only W IV . W 0 Group 0.3ml / only normal saline. The mouse is placed in a special plastic cylinder, the tail is exposed outside the cylinder, and the indoor temperature is kept at (20±1℃). After the animal is stable for 30 minutes, the pain is measured by the radiant heat tail flick method, and a special lampshade is used to make it emit A light beam with a diameter of 2mm is irradiated at a distance of about 2-3cm from the end of the tail, and the voltage of the light source is adjusted to make the late...

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Abstract

The invention discloses a preparation method of eupolyphaga seu steleophagadry dry powder extract. The method adopted by the invention includes water extraction method or enzymolysis method. The eupolyphaga seu steleophagadry dry powder is firstly processed with water extraction or enzymolysis; the water extraction liquid or enzymolysis liquid is chromatographically separated through Sephadex G25column to obtain the polypeptide component series, and then the polypeptide component is frozen and dried to prepare frozen dry powder. The model evaluation of mouse ear swelling indicates that the polypeptide component series of eupolyphaga seu steleophagadry dry powder extract has obvious inhibition effect on pain. The experiment of feeding mice for a month indicates that the polypeptide component has no toxicity and no dependence. The invention provides the application of polypeptide component series of eupolyphaga seu steleophagadry dry powder extract in the preparation of analgesic medicines.

Description

Technical field [0001] The present invention relates to traditional Chinese medicine, in particular to a process for extracting effective parts of analgesia from dry powder of soft-shelled turtle insects, a series of polypeptide components obtained by the extraction process, and the application of the polypeptide components in preparing analgesic drugs. Background technique [0002] Pain is not only a comorbidity of many diseases, but also has been regarded as a kind of disease. In terms of disease, the decline in quality of life caused by various pains affects the largest number of people. Since effective analgesics are often associated with dependence, the invention of new dependence-free analgesics has important value. [0003] Soft-shelled turtle and dry soft-shelled soft-shelled turtle are widely used in traditional Chinese medicine to treat herpes zoster. Based on the intense pain associated with herpes zoster and the therapeutic effect of the dry soft-shelled turtle powder...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K38/08A61K38/10A61K9/19A61P25/04A61P29/00C12P21/02
Inventor 彭师奇赵明魏蕾李春波毛伟叶伟东
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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