Lactobacillus casei bacteriocin and use thereof in feed

A technology of Lactobacillus casei, bacteriocin, applied in the application, bacteria, animal feed and other directions, can solve the problem of limited commercial products

Active Publication Date: 2011-05-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The application of bacteriocins as antibiotic substitutes in feed has a huge application space, but at present, domestic and foreign scholars' research on th

Method used

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  • Lactobacillus casei bacteriocin and use thereof in feed
  • Lactobacillus casei bacteriocin and use thereof in feed
  • Lactobacillus casei bacteriocin and use thereof in feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and purification of bacteriocin produced by Lactobacillus casei (Lactobacillus casei) lyT-7

[0024] 1. Antibacterial activity analysis of Lactobacillus casei (Lactobacillus casei) lyT-7

[0025] Antibacterial activity analysis method: under the incident light condition of 600λ, adjust the optical density value of the indicator bacteria (E. A puncher is used to punch holes in the culture medium, adding the fermentation supernatant of Lactobacillus casei (Lactobacillus casei) lyT-7, diffusing at room temperature for 30 minutes, cultivating at 30°C for 16-18 hours, and observing the inhibition zone.

[0026] Lactobacillus casei (Lactobacillus casei) lyT-7 was inserted into the improved MRS culture medium, and its ingredients were: soybean peptone 10.0g, beef extract 10.0g, yeast extract 5g, glucose 20.0g, K 2 HPO 4 2.0g, 2.0g diammonium citrate, 5.0g sodium acetate, MgSO 4 .7H 2 O 0.58g, MnSO 4 .4H 2 O 0.25g, Tween 80 1ml, agar 15.0g, pH natural...

Embodiment 2

[0048] Example 2 Application of Bacteriocin Crude Extract in Feed

[0049] The crude extract was subjected to a plate antibacterial test, the diameter of the inhibition zone was measured, and the potency of the active ingredients in the crude extract was converted to 13095 IU / mL according to the standard curve of the inhibition zone.

[0050] Pig feed formula: powder feed formula: corn flour 58%, wheat bran 17%, bean cake 20%, fish meal 4%, bone meal 0.5%, salt 0.5%

[0051] Add 2.5 mg (Tc1), 5 mg (Tc2), and 10 mg (Tc3) of the effective antibacterial components of the crude extract per 1000 g of feed. Store in natural environment (room temperature, humidity is 85%) and deterioration condition (high temperature, high humidity 37 ℃, humidity above 90%) respectively, do blank control (CKc) with the feed that does not add any antibacterial substance, every Samples were counted for bacteria every 7 days. As a result, it was found that the crude extract of bacteriocin could effect...

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Abstract

The invention discloses lactobacillus casei bacteriocin and use thereof in feed. The lactobacillus casei provided by the invention is separated from Tibet plateau traditional fermented yak yogurt, and the produced bacteriocin is obtained by ammonium sulfate precipitation, Sephadex G-100 gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC). The bacteriocin has thermal and acid stability, can be degraded by protease and has a wide antibacterial spectrum. When the bacteriocin is added into feed, and the growth of bacteria can be inhibited. The bacteriocin has a bright prospect in use as a feed additive.

Description

technical field [0001] The invention relates to a bacteriocin produced by Lactobacillus casei and its application in feed. Background technique [0002] Lactobacillus bacteriocins (Bacteriocins of LAB) refer to a class of proteins, polypeptides or precursor polypeptides with antibacterial activity synthesized by ribosomes during the metabolic process of lactic acid bacteria (Klaenhammer, TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS. Microbiology Review[J].1993.12:39~85), it is of great significance in inhibiting various pathogenic microorganisms and food spoilage bacteria. [0003] In the past ten years, my country's animal husbandry has continued to develop steadily and has become a pillar industry of the national economy. my country's meat and eggs rank first in the world; milk ranks fifth in the world. However, while veterinary drugs and feed additives promote the development of aquaculture production, they also bring about serious negative impact...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00A23K1/16C12R1/245A23L3/3526
Inventor 杨志荣罗璠冯甦张杰赵建侯若彤孙群
Owner SICHUAN UNIV
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