Preparation method of small water turtle anti-tumor polypeptide

An anti-tumor and money technology, which is applied in the field of preparation of anti-tumor polypeptides of the tortoise, can solve the problems of large toxic and side effects and unsatisfactory treatment effect.
CN104630318AActive Publication Date: 2015-05-20广东省几何细胞生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
广东省几何细胞生物科技有限公司
Publication Date
2015-05-20

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Abstract

The invention provides a preparation method of a small water turtle anti-tumor polypeptide. The method comprises the following steps: mixing 3 to 20g of meat paste of small water turtle and ultrapure water; agitating to obtain a mixed solution at concentration of 10 to 50% (w / v); adding trypsin to perform enzymolysis; deactivating enzyme after enzymolysis; cooling until reaching room temperature; centrifuging enzymatic hydrolysate; collecting supernate; sequentially filtering through ultrafiltration membranes which respectively have molecular cut off of 10KD, 5KD and 3KD, so as to obtain three enzymatic hydrolysates; performing sephadex G-15 column chromatography for 0-3K enzymatic hydrolysate; eluting with water; collecting four polypeptide components under a certain detection wavelength so as to obtain small water turtle anti-tumor polypeptide. The prepared small water turtle anti-tumor polypeptide is beneficial for the development and utilization of anti-tumor drugs and health foods.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a money turtle anti-tumor polypeptide. Background technique

[0002] The money turtle (Cuora Trifasciata), also known as the three-lined box turtle, has extremely high medicinal value. In ancient Eastern Han Dynasty "Shen Nong's Materia Medica" and "Shan Hai Jing" in the Warring States Period, there are records of the edible and medicinal use of turtles; the golden turtle has obvious effects on detoxification, anti-inflammatory, liver and kidney, and is often used for health care and beauty, prevention and treatment of tumors and longevity. One of the main medicines for fitness. The active factor contained in the money turtle is relatively high, which has a certain effect on the treatment of thrombocytopenia and cell death, and can inhibit the growth of cancer cells and promote blood circulation in the human body.

[0003] Biologically active pep...

Claims

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