Dracaena cochinchinensis callus seedling culture method

A technology of Dracaena glabrata and callus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as increasing callus induction rate and reducing browning rate, and achieve large-scale seedling cultivation problems, reduced browning, and high survival rate

Active Publication Date: 2018-08-21
广西洪葛生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, the difficulty of the tissue culture of Dracaena flavinica is: problem one, to explore the optimal hormone factor and condition of callus induction of Dracaena glabrata so as to increase the induction rate of callus; problem two, by adding Anti-browning substances, changing the culture environment, etc. to find better ways to inhibit browning, in order to effectively reduce the browning rate, and establish a high-quality Dracaena callus culture system; Question 3: Explore Dracaena Callus differentiation, optimal hormone factors and conditions for rooting culture to increase the proliferation coefficient and rooting rate of Dracaena glabrata clustered shoots
At present, there are only two comparati...

Method used

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  • Dracaena cochinchinensis callus seedling culture method
  • Dracaena cochinchinensis callus seedling culture method
  • Dracaena cochinchinensis callus seedling culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) Selection of explants: take the sterile test tube bacteria of Dracaena glabrata as explants.

[0020] (2) Culture medium induction to obtain callus: the obtained explants were placed in the ultra-clean workbench, cut a 0.5 cm section with a scalpel, inoculated into MS medium, and cultured in a dark room at a temperature of 23-27 ° C. A large number of calli were obtained by culturing for 40 days under the conditions of culture, in which 1.0 mg / L of 6-benzyl adenine 6-BA, 0.2 mg / L of naphthalene acetic acid NAA, and 1.0 mg / L of dichloromethane were added to the MS medium Phenoxyacetic acid 2.4-D, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8.

[0021] (3) Callus proliferation culture: the callus obtained in step (2) is placed in MS medium, at a culture temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 8-10 hours / day. Under culture for 40 days to obtain differentiated callus parts, wherein th...

Embodiment 2

[0026] Add 2.0mg / L thioridazine TDZ, 0.2mg / L kinetin KT, 0.5mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g in the MS differentiation medium used in step 4 / L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.

Embodiment 3

[0028] Add 1.5mg / L thioridazine TDZ, 0.2mg / L kinetin KT, 0.3mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g in the MS differentiation medium used in step 4 / L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.

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Abstract

The invention provides a Dracaena cochinchinensis callus seedling culture method. The Dracaena cochinchinensis callus seedling culture method comprises following steps: 1, explant selection; 2, induction of callus; 3, callus propagation culture; 4, callus differentiation culture, wherein 1 to 3mg/L thioridazine TDZ, 0.1 to 0.5mg/L of kinetin KT, 0.1 to 0.5mg/L of brassinolide, and 2mg/L of polyvinylpyrrolidone PVP are added into a MS differentiation culture medium; 5, rooting culture, wherein 0.5 to 1.0mg/L of naphthylacetic acid NAA and 2g/L of active carbon are added into a 1/2MS rooting culture medium; and 6, hardening seedling and transplanting. The Dracaena cochinchinensis multiple shoot propagation coefficient is increased to be 5 to 12 times of an original value, obtained multiple shoots are strong, rooting is easy to realize after inoculation to a rooting medium, rooting rate is 90% or higher, transplanting survival rate is 95% or higher, and a large amount of Dracaena cochinchinensis seedlings suitable for plantation can be cultured in a short time.

Description

technical field [0001] The invention relates to the technical field of seedling asexual propagation, in particular to a method for forming seedlings from callus of Dracaena glabrata. Background technique [0002] Dracaena cochinchinensis (Lour.) S.C.Chen belongs to the genus Dracaena of Liliaceae, also known as Millennium Tree, Dracaena cochinchinensis, and Dracaena cochinchinensis. Blood stasis, swelling and pain relief, astringent and hemostatic effects, mainly used for bruises, bleeding from wounds and abdominal cramps, etc. It is a national key protected wild plant, an endangered and rare species. Sword leaf dracaena has high medicinal value and is a traditional Chinese medicinal material in my country. . Dracaena resources in our country are getting less and less, and the consumption of traditional Chinese medicine resources has risen sharply. In recent years, the market demand for dracaena has been increasing, which has led to the devastating logging of wild resource...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程玲
Owner 广西洪葛生物科技有限公司
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