A kind of method that Dracaena glabra callus becomes seedling
A technology of Dracaena glabra and callus, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as increasing callus induction rate and reducing browning rate, and achieve large-scale solution Seedling problems, browning reduction, browning prevention effect
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Embodiment 1
[0020] (1) Selection of explants: take the aseptic test-tube seedlings of Dracaena flavescens as explants.
[0021] (2) Culture medium induction to obtain callus: the obtained explants were placed in the ultra-clean workbench, cut a 0.5 cm section with a scalpel, inoculated into MS medium, and cultured in a dark room at a temperature of 23-27 ° C. A large number of calli were obtained by culturing for 40 days under the conditions of culture, in which 1.0 mg / L of 6-benzyl adenine 6-BA, 0.2 mg / L of naphthalene acetic acid NAA, and 1.0 mg / L of dichloromethane were added to the MS medium Phenoxyacetic acid 2.4-D, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8.
[0022] (3) Callus proliferation culture: the callus obtained in step (2) is placed in MS medium, at a culture temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 8-10 hours / day. Under culture for 40 days to obtain differentiated callus parts, wherein...
Embodiment 2
[0027] Add 2.0mg / L thiadizuron TDZ, 0.2mg / L kinetin KT, 0.5mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g / L to the MS differentiation medium used in step 4. L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.
Embodiment 3
[0029] In the MS differentiation medium used in step 4, add the kinetin KT of 1.5mg / L thiadizuron TDZ, 0.2mg / L, 0.3mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g / L L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.
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