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A kind of method that Dracaena glabra callus becomes seedling

A technology of Dracaena glabra and callus, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as increasing callus induction rate and reducing browning rate, and achieve large-scale solution Seedling problems, browning reduction, browning prevention effect

Active Publication Date: 2021-09-28
广西洪葛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, the difficulty of the tissue culture of Dracaena flavinica is: problem one, to explore the optimal hormone factor and condition of callus induction of Dracaena glabrata so as to increase the induction rate of callus; problem two, by adding Anti-browning substances, changing the culture environment, etc. to find better ways to inhibit browning, in order to effectively reduce the browning rate, and establish a high-quality Dracaena callus culture system; Question 3: Explore Dracaena Callus differentiation, optimal hormone factors and conditions for rooting culture to increase the proliferation coefficient and rooting rate of Dracaena glabrata clustered shoots
At present, there are only two comparative documents on the propagation of Dracaena glabrata using tissue culture technology. Comparative document 1: the patent application number is "CN201510755473.7", and the patent name is "A kind of Dracaena glabrata loose callus induction Method” is the solution to the above-mentioned technical problem 1; reference document 2: the patent application number is “CN201410735185.0”, and the patent name is “A method for inducing callus and preventing browning of Dracaena glabrata”, which solves all Described technical problem two; the above two comparative documents have not explored and solved the best hormone factors and conditions of the differentiation of the follow-up Dracaena callus, rooting culture in the third problem

Method used

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  • A kind of method that Dracaena glabra callus becomes seedling
  • A kind of method that Dracaena glabra callus becomes seedling
  • A kind of method that Dracaena glabra callus becomes seedling

Examples

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Embodiment 1

[0020] (1) Selection of explants: take the aseptic test-tube seedlings of Dracaena flavescens as explants.

[0021] (2) Culture medium induction to obtain callus: the obtained explants were placed in the ultra-clean workbench, cut a 0.5 cm section with a scalpel, inoculated into MS medium, and cultured in a dark room at a temperature of 23-27 ° C. A large number of calli were obtained by culturing for 40 days under the conditions of culture, in which 1.0 mg / L of 6-benzyl adenine 6-BA, 0.2 mg / L of naphthalene acetic acid NAA, and 1.0 mg / L of dichloromethane were added to the MS medium Phenoxyacetic acid 2.4-D, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8.

[0022] (3) Callus proliferation culture: the callus obtained in step (2) is placed in MS medium, at a culture temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 8-10 hours / day. Under culture for 40 days to obtain differentiated callus parts, wherein...

Embodiment 2

[0027] Add 2.0mg / L thiadizuron TDZ, 0.2mg / L kinetin KT, 0.5mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g / L to the MS differentiation medium used in step 4. L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.

Embodiment 3

[0029] In the MS differentiation medium used in step 4, add the kinetin KT of 1.5mg / L thiadizuron TDZ, 0.2mg / L, 0.3mg / L brassinolide, 2mg / L polyvinylpyrrolidone PVP, 30g / L L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other operation steps are all identical with described embodiment 1.

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Abstract

The invention provides a method for seedling formation of Dracaena glabrata callus, which specifically comprises the following steps: (1) selection of explants; (2) induction of callus; (3) callus proliferation and cultivation (4) Callus differentiation culture, adding 1-3mg / L thiadizuron TDZ, 0.1-0.5mg / L kinetin KT, 0.1-0.5mg / L brassinolide and 2mg / L polyvinylpyrrolidone PVP; (5) Rooting culture, add 0.5-1.0mg / L NAA and 2g / L activated carbon to 1 / 2MS rooting medium; (6) Hardening and transplanting. The multiplication coefficient of clustered buds of Dracaena saponifera reaches 5-12 times. The clustered buds are strong and easy to take root after being inoculated into the rooting medium. The rooting rate is over 90%, and the transplanting survival rate is over 95%. It can be cultivated in a short time. A large number of dracaena seedlings suitable for cultivation have been produced.

Description

technical field [0001] The invention relates to the technical field of seedling asexual propagation, in particular to a method for forming seedlings from callus of Dracaena glabrata. Background technique [0002] Dracaena cochinchinensis (Lour.) S.C.Chen belongs to the genus Dracaena of Liliaceae, also known as Millennium Tree, Dracaena cochinchinensis, and Dracaena cochinchinensis. Blood stasis, swelling and pain relief, astringent and hemostatic effects, mainly used for bruises, bleeding from wounds and abdominal cramps, etc. It is a national key protected wild plant, an endangered and rare species. Sword leaf dracaena has high medicinal value and is a traditional Chinese medicinal material in my country. Dracaena resources in our country are getting less and less, and the consumption of traditional Chinese medicine resources has risen sharply. In recent years, the market demand for dracaena has been increasing, which has led to the devastating logging of wild resources o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程玲
Owner 广西洪葛生物科技有限公司
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