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Rapid propagation method for tissue culture of nervilia fordii solid bulb

The invention is a technique for cultivating blue sky sunflower balls and tissue, which can be applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of low reproduction coefficient and depletion of wild blue sky sunflower resources, so as to reduce the cultivation steps, solve the problem of large-scale seedling raising, The effect of reducing training costs

Inactive Publication Date: 2018-07-20
佛山市元诺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue skyflower mainly reproduces by bulbs, but the reproduction coefficient is extremely low. In addition, people have been digging extinctly for many years, which has led to the depletion of wild blue skyflower resources.
However, the demand in domestic and foreign markets is increasing year by year, and Qingtiankui is far from meeting the demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0020] (1) Extraction and disinfection of explants: As the explants, the bulbs of the blue skyflower are sterilized. First, 3-5 drops of detergent are dripped into a beaker with 50ml of tap water, and the explants are put into the beaker and stirred gently. 5min, then use cotton to clean the dirt on the surface of the explant, rinse with tap water for 20-30min; move the ultra-clean bench, soak in 75v / v% ethanol for 30s, rinse with sterile water, and then add 1-2 Sterilize 50ml of Tween-20 with 0.1v / v% mercuric chloride for 1-2min, rinse with sterile water for 3-5 times to obtain sterile explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure ;

[0021] (2) Acquisition of sterile rhizomes: Place the sterilized explants in the induction medium for adventitious bud induction, at a temperature ...

Embodiment 2

[0028] Another example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0029] Other conditions are the same as embodiment 1, only the following content is different:

[0030] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - Benzyl adenine, kinetin (KT) 0.3mg·L-1, naphthaleneacetic acid 0.3mg·L-1, strawberry juice 100g·L-1, medium pH value 6.8; corm induction coefficient 23.9.

[0031] In step (4), the transplanting survival rate was 93.8%.

Embodiment 3

[0033] Another example of the rapid propagation method of the blue skyflower bulb tissue culture of the present invention comprises the following steps:

[0034] Other conditions are the same as embodiment 1, only the following content is different:

[0035] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - Benzyl adenine, kinetin (KT) 0.5 mg L-1, naphthalene acetic acid 0.5 mg L-1, strawberry juice 150 g L-1, medium pH value 6.8; bulb induction coefficient 23.4.

[0036] In step (4), the transplanting survival rate was 91.6%.

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PUM

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Abstract

The invention discloses a rapid propagation method for tissue culture of a nervilia fordii solid bulb. The method is characterized by comprising the following steps that (1) an explant is obtained anddisinfected; (2) a sterile rhizome is obtained; and (3) integrative cultivation is conducted on the rhizome reproduction and the solid bulb; (4) hardening-seedling and transplanting are conducted onthe solid bulb. Compared with the prior art, the rapid propagation method for the tissue culture of the nervilia fordii solid bulb has the advantages that biotechnology is used to rapidly propagate the tissue of the s nervilia fordii solid bulb, a large number of nervilia fordii seedlings capable of being cultivated in a field in a short time, the breeding coefficient and seedling quality of nervilia fordii are significantly improved, large-scale production is achieved, and the needs of production are met.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of blue skyflower bulbs. Background technique [0002] Blue Sky Kwai is the leaf or bulbous leaf of orchid plant Nerviliafordii (Hance) Schitr, also known as unifoliate, uniped lotus, pearl leaf, falling jack, iron hat, samosa, and green lotus. Flat in nature, bitter in the mouth, sweet, cool. Astragalus is a rare and endangered plant in my country. It has the functions of clearing the lung and relieving cough, strengthening the spleen and eliminating stagnation, clearing away heat and detoxification, eliminating stagnation and dispelling pain. Scrofula, sores, swollen toxins, bruises, etc. The blue skyflower is mainly propagated by bulbs, but the reproduction coefficient is extremely low. In addition, the extinction-style excavation by people over the years has led to the depletion of wild blue skyflower resources. However, the demand in domestic and foreign markets is increasing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 袁萍
Owner 佛山市元诺科技有限公司
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