Tissue culture rapid propagation method for nervilis fordii corm
A technology for cultivating blue skyflower balls and tissues, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as low reproduction coefficient and exhaustion of wild blue skyflower resources, reduce cultivation steps, solve large-scale seedling cultivation, The effect of reducing the cost of cultivation
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[0018] Example 1
[0019] An example of the method for rapid propagation of Cymbidium aquilinum bulb tissue culture according to the present invention includes the following steps:
[0020] (1) Extraction and disinfection of explants: The bulbs of Qingtiankui as explants are disinfected. First, drip 3-5 drops of detergent into a beaker with 50ml tap water, and put the explants in the beaker and stir gently 5min, clean the dirt on the surface of the explant with cotton, rinse with tap water for 20-30min; move the ultra-clean workbench, soak in 75v / v% ethanol for 30s, rinse with sterile water, and then add 1-2 Drop Tween-20 with a concentration of 0.1v / v% mercury in 50ml, sterilize for 1-2 minutes, rinse with sterile water 3-5 times to obtain sterile explants, where the sterile water is distilled water sterilized by high temperature pressure ;
[0021] (2) Obtaining sterile rhizomes: place the sterilized explants in the induction medium for adventitious bud induction, at a temperatur...
Example Embodiment
[0027] Example 2
[0028] Another example of the method for rapid propagation of Cymbidium aquilinum bulb tissue culture according to the present invention includes the following steps:
[0029] Other conditions are the same as in Example 1, except for the following:
[0030] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, supplemented with 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 6 -Benzyl adenine, 0.3 mg·L-1 kinetin (KT), 0.3 mg·L-1 naphthalene acetic acid, coconut juice 100 g·L-1, the medium pH is 6.8; the bulb induction coefficient is 23.9.
[0031] In step (4), the transplant survival rate was 93.8%.
Example Embodiment
[0032] Example 3
[0033] Another example of the method for rapid propagation of Cymbidium aquilinum bulb tissue culture according to the present invention includes the following steps:
[0034] Other conditions are the same as in Example 1, except for the following:
[0035] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, supplemented with 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 6 -Benzyl adenine, 0.5 mg·L-1 kinetin (KT), 0.5 mg·L-1 naphthalene acetic acid, 150 g·L-1 coconut juice, medium pH value 6.8; bulb induction coefficient 23.4.
[0036] In step (4), the transplant survival rate was 91.6%.
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