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Tissue culture rapid propagation method for nervilis fordii corm

A technology for cultivating blue skyflower balls and tissues, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as low reproduction coefficient and exhaustion of wild blue skyflower resources, reduce cultivation steps, solve large-scale seedling cultivation, The effect of reducing the cost of cultivation

Inactive Publication Date: 2018-09-28
佛山市元诺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue skyflower mainly reproduces by bulbs, but the reproduction coefficient is extremely low. In addition, people have been digging extinctly for many years, which has led to the depletion of wild blue skyflower resources.
However, the demand in domestic and foreign markets is increasing year by year, and Qingtiankui is far from meeting the demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0020] (1) Extraction and disinfection of explants: As the explants, the bulbs of the blue skyflower are sterilized. First, 3-5 drops of detergent are dripped into a beaker with 50ml of tap water, and the explants are put into the beaker and stirred gently. 5min, then use cotton to clean the dirt on the surface of the explant, rinse with tap water for 20-30min; move the ultra-clean bench, soak in 75v / v% ethanol for 30s, rinse with sterile water, and then add 1-2 Sterilize 50ml of Tween-20 with 0.1v / v% mercuric chloride for 1-2min, rinse with sterile water for 3-5 times to obtain sterile explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure ;

[0021] (2) Acquisition of sterile rhizomes: Place the sterilized explants in the induction medium for adventitious bud induction, at a temperature ...

Embodiment 2

[0028] Another example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0029] Other conditions are the same as embodiment 1, only the following content is different:

[0030] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - benzyl adenine, kinetin (KT) 0.3 mg L-1, naphthalene acetic acid 0.3 mg L-1, coconut milk 100 g L-1, medium pH value 6.8; corm induction coefficient 23.9.

[0031] In step (4), the transplanting survival rate was 93.8%.

Embodiment 3

[0033] Another example of the rapid propagation method of the blue skyflower bulb tissue culture of the present invention comprises the following steps:

[0034] Other conditions are the same as embodiment 1, only the following content is different:

[0035] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - benzyl adenine, kinetin (KT) 0.5 mg L-1, naphthalene acetic acid 0.5 mg L-1, coconut milk 150 g L-1, medium pH value 6.8; corm induction coefficient 23.4.

[0036] In step (4), the transplanting survival rate was 91.6%.

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation method for nervilis fordii corm. The tissue culture rapid propagation method is characterized by comprising the following steps: firstly, material drawing and disinfection of an explant; secondly, obtaining of sterile rhizomes; thirdly, integrated culture of rhizome propagation and corm induction; fourthly, acclimatization and transplantation of the corm. Compared with the prior art, the technical scheme provided by the invention has the beneficial effects that tissue culture rapid propagation of the nervilis fordii corm is realized bya biotechnology, and mass nervilis fordii seedlings which can be cultivated in the field are cultivated in a short time; a propagation coefficient and seedling quality of the nervilis fordii seedlings are remarkably improved, scale production is realized and the requirements of production are met.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of blue skyflower bulbs. Background technique [0002] Blue Sky Kwai is the leaf or bulbous leaf of orchid plant Nerviliafordii (Hance) Schitr, also known as unifoliate, uniped lotus, pearl leaf, falling jack, iron hat, samosa, and green lotus. Flat in nature, bitter in the mouth, sweet, cool. Astragalus is a rare and endangered plant in my country. It has the functions of clearing the lung and relieving cough, strengthening the spleen and eliminating stagnation, clearing away heat and detoxification, eliminating stagnation and dispelling pain. Scrofula, sores, swollen toxins, bruises, etc. The blue skyflower is mainly propagated by bulbs, but the reproduction coefficient is extremely low. In addition, the extinction-style excavation by people over the years has led to the depletion of wild blue skyflower resources. However, the demand in domestic and foreign markets is increasing...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 袁萍
Owner 佛山市元诺科技有限公司
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