Tissue culture rapid propagation method of ploygonatum cirrhifolium royle

A technology of tissue culture, rapid propagation, and Polygonatum chinensis is applied in the field of plant tissue culture, which can solve problems such as rare, and achieve the effects of improving seedling rate, improving reproduction coefficient and seedling quality, and cooperating closely.

Inactive Publication Date: 2018-09-18
AGRI PROD QUALITY SAFETY & DETECTION TECH RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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[0031] Example 1

[0032] A method for tissue culture and rapid propagation of Polygonatum leaf roll, including the following steps:

[0033] (1) Selection and disinfection of explants: take the side buds of Polygonatum sylvestris as explants, soak in 2% detergent aqueous solution for 5 minutes, rinse with linear tap water for 15 minutes, and add 2 drops of Tween-20 100mL0.1 Disinfect with% mercury for 8 minutes, rinse with sterile water for 3 times, and finally remove the surface moisture with sterile filter paper to obtain explants, where the sterile water is autoclaved distilled water;

[0034] (2) Induction of the first generation of explants to obtain sterile test tube seedlings: Place the explants obtained in step (1) in MS medium at a culture temperature of 23℃, light intensity of 1500lux, and light time of 8 hours / day Cultivate for 30 days under the conditions to obtain a sterile test tube plantlet, in which 1.0 mg / L 6-benzyl adenine 6-BA, 0.2 mg / L indole acetate IAA, 30 g / L...

Example Embodiment

[0039] Example 2

[0040] A method for tissue culture and rapid propagation of Polygonatum leaf roll, including the following steps:

[0041] (1) Selection and disinfection of explants: Take the side buds of Polygonatum serrata as explants, soak them in 2% detergent solution for 5 minutes, rinse with linear tap water for 30 minutes, and add 3 drops of Tween-20 to 100mL0.1 Disinfect with% mercury for 10 minutes, rinse with sterile water for 5 times, and finally remove the surface moisture with sterile filter paper to obtain explants with distilled water;

[0042] (2) Induction of the first generation of explants to obtain sterile test tube plantlets: Place the explants obtained in step (1) in MS medium at a culture temperature of 27°C, light intensity of 1500 lux, and light time of 10 hours / day. Cultivate for 30 days under the conditions to obtain a sterile test tube seedling, in which 1.5 mg / L 6-benzyl adenine 6-BA, 0.2 mg / L indole acetate IAA, 30 g / L sucrose and 5 g / L were added t...

Example Embodiment

[0047] Example 3

[0048] A method for tissue culture and rapid propagation of Polygonatum leaf roll, including the following steps:

[0049] (1) Selection and disinfection of explants: take the side buds of Polygonatum vulgaris as explants, soak them in a 2% detergent solution for 5 minutes, rinse with linear tap water for 20 minutes, and add 3 drops of Tween-20 to 100mL0.1 Disinfect with% mercury for 9 minutes, rinse with sterile water 4 times, and finally remove the surface moisture with sterile filter paper to obtain explants, where the sterile water is autoclaved distilled water;

[0050] (2) Induction of the first generation of explants to obtain sterile test tube seedlings: Place the explants obtained in step (1) in MS medium at a culture temperature of 25°C, light intensity of 1500 lux, and light time of 9 hours / day Cultivate for 30 days under the conditions to obtain a sterile test tube plantlet, in which 1.5 mg / L 6-benzyl adenine 6-BA, 0.5 mg / L indole acetate IAA, 30 g / L ...

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Abstract

The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of ploygonatum cirrhifolium royle. The tissue culture rapid propagationmethod of the ploygonatum cirrhifolium royle comprises the following steps: (1) selection and disinfection of explants, (2) obtaining sterile test-tube seedlings by primary generation of induction ofexplants, (3) rapid propagation of multiple shoots of the test-tube seedlings, (4) strong seedling culture of the multiple shoots, (5) rooting culture and (6) acclimatization and transplant. The tissue culture rapid propagation method can cultivate a large number of seedlings, capable of being cultivated in a large number, of the ploygonatum cirrhifolium royle, significantly improve reproductivecoefficient and seedling quality of the seedlings of the ploygonatum cirrhifolium royle, can realize large-scale production, and meets the needs of production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Polygonatum curcuma. Background technique [0002] Polygonatum cirrhifolium (Polygonatum cirrhifolium), which belongs to Liliaceae (Liliaceae) Polygonatum (Ploygonatum) perennial herb, is commonly used medicine and food homologous traditional Chinese medicine, Tibetan name transliteration "Kekou pear". Sweet and flat in nature and flavor, it has the functions of nourishing qi and nourishing yin, lowering blood pressure, lowering blood sugar, lowering blood fat, moistening lung, nourishing kidney, strengthening spleen and so on. It is known as "immortality and longevity" medicine. The "Shen Nong's Materia Medica" of the Han Dynasty, the "Compendium of Materia Medica" of the Ming Dynasty, and the famous Tibetan medicine "Jingzhu Materia Medica" all have records of its medicinal and dietary tonic effects. Polygonatum...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 劳水兵刘丽辉莫仁甫覃国新周其峰杨玉霞何洁吴玉东
Owner AGRI PROD QUALITY SAFETY & DETECTION TECH RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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