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Tissue culture rapid propagation method of ploygonatum cirrhifolium royle

A technology of tissue culture, rapid propagation, and Polygonatum chinensis is applied in the field of plant tissue culture, which can solve problems such as rare, and achieve the effects of improving seedling rate, improving reproduction coefficient and seedling quality, and cooperating closely.

Inactive Publication Date: 2018-09-18
AGRI PROD QUALITY SAFETY & DETECTION TECH RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Embodiment 1

[0032] A method for tissue culture and rapid propagation of Polygonatum curcuma, comprising the steps of:

[0033] (1) Selection and disinfection of explants: Take Polygonatum curcuma lateral buds as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 15 minutes, add 2 drops of Tween-20 to 100mL0.1 % mercuric chloride disinfection for 8 minutes, rinsed with sterile water for 3 times, and finally removed the surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0034] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in MS medium, and the culture temperature is 23° C., the light intensity is 1500 lux, and the light time is 8 hours / day. Cultivate under condition for 30 days to obtain sterile test-tube plantlets, wherein in the MS medium, add 1.0mg / L of 6-benzy...

Embodiment 2

[0040] A method for tissue culture and rapid propagation of Polygonatum curcuma, comprising the steps of:

[0041] (1) Selection and disinfection of explants: Take Polygonatum curcuma lateral buds as explants, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 30 minutes, add 3 drops of Tween-20 in 100 mL0.1 % mercuric chloride disinfection for 10 minutes, rinsed with sterile water for 5 times, and finally removed the surface moisture with sterile filter paper to obtain the explants, including distilled water;

[0042] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in MS medium, and the culture temperature is 27 ° C, the light intensity is 1500 lux, and the light time is 10 hours / day. Cultivate under condition for 30 days to obtain sterile test-tube plantlets, wherein in the MS medium, add 1.5mg / L of 6-benzyl adenine 6-BA, 0.2mg / L of indole acetic acid IAA,...

Embodiment 3

[0048] A method for tissue culture and rapid propagation of Polygonatum curcuma, comprising the steps of:

[0049] (1) Selection and disinfection of explants: take the lateral buds of Polygonatum curcuma as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 20 minutes, add 3 drops of Tween-20 to 100 mL of 0.1 % mercuric chloride disinfection for 9 minutes, rinsed with sterile water for 4 times, and finally removed the surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0050] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in MS medium, and the culture temperature is 25° C., the light intensity is 1500 lux, and the light time is 9 hours / day. Cultivate under condition for 30 days to obtain sterile test-tube plantlets, wherein in the MS medium, add 1.5mg / ...

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Abstract

The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of ploygonatum cirrhifolium royle. The tissue culture rapid propagationmethod of the ploygonatum cirrhifolium royle comprises the following steps: (1) selection and disinfection of explants, (2) obtaining sterile test-tube seedlings by primary generation of induction ofexplants, (3) rapid propagation of multiple shoots of the test-tube seedlings, (4) strong seedling culture of the multiple shoots, (5) rooting culture and (6) acclimatization and transplant. The tissue culture rapid propagation method can cultivate a large number of seedlings, capable of being cultivated in a large number, of the ploygonatum cirrhifolium royle, significantly improve reproductivecoefficient and seedling quality of the seedlings of the ploygonatum cirrhifolium royle, can realize large-scale production, and meets the needs of production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Polygonatum curcuma. Background technique [0002] Polygonatum cirrhifolium (Polygonatum cirrhifolium), which belongs to Liliaceae (Liliaceae) Polygonatum (Ploygonatum) perennial herb, is commonly used medicine and food homologous traditional Chinese medicine, Tibetan name transliteration "Kekou pear". Sweet and flat in nature and flavor, it has the functions of nourishing qi and nourishing yin, lowering blood pressure, lowering blood sugar, lowering blood fat, moistening lung, nourishing kidney, strengthening spleen and so on. It is known as "immortality and longevity" medicine. The "Shen Nong's Materia Medica" of the Han Dynasty, the "Compendium of Materia Medica" of the Ming Dynasty, and the famous Tibetan medicine "Jingzhu Materia Medica" all have records of its medicinal and dietary tonic effects. Polygonatum...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 劳水兵刘丽辉莫仁甫覃国新周其峰杨玉霞何洁吴玉东
Owner AGRI PROD QUALITY SAFETY & DETECTION TECH RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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