Subprostrate sophora root tissue culture seedling industrialization production method

A production method and an industrialized technology are applied in the field of industrialized production of tissue culture seedlings of broad bean root, which can solve the problems of low seed yield, serious diseases and insect pests of broad bean root, difficulty in realizing large-scale cultivation, etc. Effects of reproduction coefficient and seedling quality, reducing cultivation cost

Active Publication Date: 2012-10-10
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, broad bean roots mainly rely on seeds for reproduction, but during the flowering and pod setting stage, broad bean roots are severely damaged by diseases and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] A factory production method of tissue culture seedlings of broad bean root includes the following steps:

[0018] (1) Reproduction and rehabilitation integrated culture: The reproduction and rehabilitation integrated medium is: MS+1.0mg / L BA+ 0.3mg / L KT+ 0.3mg / L IAA+0.2mg / L NAA. The integrated cultivation method for reproduction and rejuvenation is as follows: Disinfect the broad bean root seeds and inoculate them on MS+1.5mg / L BA+0.3mg / L KT+0.3mg / L IAA propagation medium to obtain clump buds, and cut the clump buds into 1.5~2.5cm of budded stems, lay flat on the surface of the integrated solid medium for reproduction and rehabilitation, and then use tweezers to press 1 / 2 of the stems into the medium to fully absorb the nutrients in the medium . After the inoculation, transfer to the culture room for 15 days. The culture conditions are culture temperature 23~27°C, light intensity 1500lux, and light time 8 hours / day. Then transfer to the scattered natura...

Example Embodiment

[0021] Example 2

[0022] A factory production method of tissue culture seedlings of broad bean root includes the following steps:

[0023] (1) Reproduction and rehabilitation integrated culture: The reproduction and rehabilitation integrated culture medium is: MS+1.5mg / L BA+ 0.3 mg / L KT+0.3mg / L IAA+0.4mg / L NAA. The integrated cultivation method for reproduction and rejuvenation is: sterilize the broad bean root seeds and inoculate them on MS+1.5mg / L BA+0.3mg / L KT+ 0.3mg / L IAA propagation medium to obtain cluster buds, and cut the cluster buds into 1.5 The ~2.5cm budded stems are laid flat on the surface of the integrated solid medium for reproduction and rehabilitation, and then 1 / 2 of the stems are pressed into the medium with tweezers, so that it can fully absorb the nutrients in the medium. After the inoculation, transfer to the culture room for 15 days. The culture conditions are culture temperature 23~27°C, light intensity 1500lux, and light time 8 hours / day. Then it was t...

Example Embodiment

[0026] Example 3

[0027] A factory production method of tissue culture seedlings of broad bean root includes the following steps:

[0028] (1) Reproduction and rehabilitation integrated culture: The reproduction and rehabilitation integrated medium is: MS+1.5mg / LBA+ 0.3 mg / L KT+ 0.3mg / L IAA + 0.5mg / L NAA. The integrated cultivation method for reproduction and rejuvenation is as follows: Disinfect the broad bean root seeds and inoculate them on MS+1.5mg / L BA+0.3mg / L KT+0.3mg / L IAA propagation medium to obtain clump buds, and cut the clump buds into 1.5~2.5cm of budded stems, lay flat on the surface of the integrated solid medium for reproduction and rehabilitation, and then use tweezers to press 1 / 2 of the stems into the medium to fully absorb the nutrients in the medium . After inoculation, transfer to the culture room for 15 days. The culture conditions are culture temperature 23~27°C, light intensity 1500lux, and light time 8 hours / day. Then, it was transferred to culture un...

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Abstract

A subprostrate sophora root tissue culture seedling industrialization production method comprises the following steps: subprostrate sophora root tissue culture seedling breeding and rejuvenation, rooting domestication and transplantation. By adopting the method, a great amount of excellent, suitably mountain-cultured subprostrate sophora root tissue culture seedling with more than 98% transplantation survival rate can be provided in short time, so as to meet production demand.

Description

[0001] technical field [0002] The invention relates to an industrial production method of plant tissue culture seedlings, in particular to an industrial production method of plant tissue culture seedlings. Background technique [0003] Broad bean root, also known as mountain bean root and southern bean root, is the dry root and rhizome of the leguminous plant Sophora tonkinensis Gapnep. It is distributed in southern my country, and Guangxi has the largest output. It has the effects of clearing fire and detoxifying, reducing swelling and relieving pain, etc. It can treat sore throat, sore throat, sore throat, swelling and pain of gums, hot cough with panting, hepatitis, constipation, jaundice, diarrhea, hemorrhoids, bald sores, scabies and mosquitoes and insects , Dog bites, etc. The pharmaceutical industry uses broad bean root as a raw material to develop a large number of injections for treating hepatitis, tablets for sore throat and anti-tumor Chinese patent medicines. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦坤华缪剑华李林轩韦范
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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