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Tissue culture and rapid propagation method for dracaena cochinchinensis

A technology of dragon blood tree and tissue culture, applied in the field of plant reproduction, can solve problems such as difficulty in realizing large-scale cultivation, limited number of seedlings, long time required, etc. The effect of mass production

Active Publication Date: 2014-02-05
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of seedling propagation, Dracaena can be propagated by seeds, cuttings or tillers, but the propagation rate of this conventional method is low, the time required is long, the number of seedlings obtained is very limited, and it is difficult to realize large-scale cultivation in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An example of the tissue culture rapid propagation method of Dracaena glabrata of the present invention, comprises the following steps:

[0024] (1) Selection and disinfection of explants: Take the seeds of Dracaena glabrata as explants, soak them in 2% detergent solution for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween 100 milliliters of 0.1% mercury liter at -20 were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0025] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the condition of 30 days, obtain sterile test-tube plantlet after seed germination, wherein in MS med...

Embodiment 2

[0031] Another example of the tissue culture rapid propagation method of Dracaena glabrata of the present invention, comprises the following steps:

[0032](1) Selection and disinfection of explants: Take the seeds of Dracaena glabrata as explants, soak them in 2% detergent solution for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween 100 milliliters of 0.1% mercury liter at -20 were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0033] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the condition of 30 days, obtain sterile test-tube plantlet after seed germination, wherein in MS...

Embodiment 3

[0039] Another example of the tissue culture rapid propagation method of Dracaena glabrata of the present invention comprises the following steps:

[0040] (1) Selection and disinfection of explants: Take the seeds of Dracaena glabrata as explants, soak them in 2% detergent solution for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween 100 milliliters of 0.1% mercury liter at -20 were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0041] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the condition of 30 days, obtain sterile test-tube plantlet after seed germination, wherein in MS...

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Abstract

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a tissue culture rapid propagation method of Dracaena glabrata. Background technique [0002] Dracaena saponifera, also known as Millennium Wood, Dracaena xenophyllus, and Dracaena cochinchinensis, is a endangered species and a second-class national protected plant. In 2003, it was classified as a vulnerable species by the International Union for Conservation of Nature and Natural Resources. It is listed on the red list and is listed as an endangered species in the Cape Verde Red Book. It is currently recognized as the base plant of domestically produced dracaena. [0003] Dried blood is a precious traditional Chinese medicine, which has been used in my country for more than 1,500 years. According to the "Compendium of Materia Medica", dragon's blood is warm, flat, sweet, salty, non-toxic, enters the blood system, and returns to the lung, spleen, and kidney meridians. It has remark...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦坤华李林轩韦莹缪剑华李翠农东新黄雪彦王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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