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A method for inducing and preventing browning of Dracaena glabra callus

A technology of callus induction and dracaena, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of limiting the efficiency of dracaena callus culture, affecting callus and infertility. Bacterial quality, difficulty in meeting large-scale production and other issues, to achieve the effect of sustainable utilization, low price and low cost

Inactive Publication Date: 2016-03-30
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Dracaena phoenix leaf has made preliminary progress in tissue culture and rapid propagation technology, but in the process of explant proliferation, induction of primary differentiation and redifferentiation, its own tissue will release brown substances into the medium, which will cause the medium to gradually brown, resulting in serious Affect the quality of callus and sterile seedlings or even die, which seriously limits the efficiency of Dracaena phoenix callus culture and is difficult to meet the needs of large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: take the seeds of Dracaena glabrata as explants, first clean the dirt on the surface of the seeds with an aqueous solution of detergent, put them in a beaker and rinse them with running water for 5-8 minutes, and then move them to an ultra-clean workbench First, soak the seeds with 75% alcohol for 30S, rinse with sterile water once, and then add 1-2 drops of Tween-20 to 100ml of 0.1% HgCl 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to blot the surface moisture of the explants before inoculating, wherein the sterile water is distilled water sterilized by high pressure;

[0019] (2) Seed induction: Inoculate the explants obtained in step (1) into the MS induction medium, at a culture temperature of 25±2°C, with a light intensity of 20~30 μmol / (m 2 s), the light time is 12h / d under the condition of cultivating 15d, the seeds germinate, and the MS induction medium for the seed induction is 6-benz...

Embodiment 2

[0024] (1) Disinfection of explants: take the seeds of Dracaena glabrata as explants, first clean the dirt on the surface of the seeds with an aqueous solution of detergent, put them in a beaker and rinse them with running water for 5-8 minutes, and then move them to an ultra-clean workbench First, soak the seeds with 75% alcohol for 30S, rinse with sterile water once, and then add 1-2 drops of Tween-20 to 100ml of 0.1% HgCl 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to blot the surface moisture of the explants before inoculating, wherein the sterile water is distilled water sterilized by high pressure;

[0025] (2) Seed induction: Inoculate the explants obtained in step (1) into the MS induction medium, at a culture temperature of 25±2°C, with a light intensity of 20~30 μmol / (m 2 s), the light time is 12h / d under the condition of cultivating 15d, the seeds germinate, and the MS induction medium for the seed induction is 6-benz...

Embodiment 3

[0030] (1) Disinfection of explants: take the seeds of Dracaena glabrata as explants, first clean the dirt on the surface of the seeds with an aqueous solution of detergent, put them in a beaker and rinse them with running water for 5-8 minutes, and then move them to an ultra-clean workbench First, soak the seeds with 75% alcohol for 30S, rinse with sterile water once, and then add 1-2 drops of Tween-20 to 100ml of 0.1% HgCl 2 Disinfect for 8 to 15 minutes, soak in sterile water for 3 times, use sterile filter paper to blot the surface moisture of the explants before inoculating, wherein the sterile water is distilled water sterilized by high pressure;

[0031] (2) Seed induction: Inoculate the explants obtained in step (1) into the MS induction medium, at a culture temperature of 25±2°C, with a light intensity of 20~30 μmol / (m 2 s), the light time is 12h / d under the condition of cultivating 15d, the seeds germinate, and the MS induction medium for the seed induction is 6-benz...

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Abstract

The invention discloses a callus induction and anti-browning method for dracaena cochinchinensis. The method comprises the following steps: (1) using dracaena cochinchinensis seeds as explants for sterilizing; (2) putting the sterilized explants into an MS minimal medium for performing induced budding; (3) putting germ-free shoots into the MS minimal medium for culturing to obtain callus; (4) putting the callus into an MS enrichment medium for culturing to obtain a large amount of callus; and (5) putting the large amount of callus into an MS anti-browning culture medium for culturing to obtain the callus with fluffy texture. The callus obtained by using the method is viridis or cream yellow, high in growing speed and difficult to brown, and can produce a large amount of callus with good growth trend and low browning rate by means of induced proliferation; and therefore, high-quality materials are provided for extracting dragon's blood in medical science, and the market requirement can be further met.

Description

technical field [0001] The invention relates to a plant callus induction and browning prevention method, in particular to a callus induction and browning prevention method of Dracaena glabrata. Background technique [0002] Dracaena cochinchinensis (Lour) S.C. Chen, also known as Millennium Wood, Blood Dracaena, and Cochin Dracaena, is a endangered species and a national second-class protected plant. It is mainly distributed in Guangxi, Yunnan, Hainan and other places. Dracaena fragrans has high medicinal value, and the blood jelly extracted from its resin is called kylin jelly. It is a traditional Chinese medicinal material and has been used in my country for more than 1,500 years. In addition, its simple and elegant shape has high ornamental value. In 2003, it was classified as a vulnerable species by the International Union for the Conservation of Nature and Natural Resources and listed on the red list. It was designated as an endangered species in the Red Book of Cape V...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韦莹韦坤华李翠李林轩王一诺黄雪彦缪剑华王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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