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A detection method for 7-hydroxy-4'-methoxyflavan and pterostilbene in total flavonoids of Dracaena glabra

A technology of stegophylla and methoxyflavan, which is applied in measurement devices, instruments, scientific instruments, etc., to achieve the effect of optimized chromatographic conditions and good reproducibility

Active Publication Date: 2016-05-11
GUILIN SANJIN PHARMACEUTICALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on the quality control standards of the original medicinal material Dracaena glabrata and its extracts focuses on the determination of the representative components of dihydrochalcones, dracaine A and dracaine B, while the main components of other categories are Active ingredients, such as the main components of flavans and stilbenes, the purification preparation and content determination of high-purity chemical reference substances have not been reported yet

Method used

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  • A detection method for 7-hydroxy-4'-methoxyflavan and pterostilbene in total flavonoids of Dracaena glabra
  • A detection method for 7-hydroxy-4'-methoxyflavan and pterostilbene in total flavonoids of Dracaena glabra
  • A detection method for 7-hydroxy-4'-methoxyflavan and pterostilbene in total flavonoids of Dracaena glabra

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Experimental program
Comparison scheme
Effect test

Embodiment 17

[0038] Embodiment 17-Hydroxy-4'-methoxyflavan chemical reference substance research

[0039] The molecular formula of 7-hydroxy-4'-methoxyflavane (7-hydroxy-4'methoxyflavane) is:

[0040]

[0041] 【Source】Dracaenacochinchinensis (Lour.) S.C.Chen, a plant of the Liliaceae family, extracts effective parts from lipid-containing wood-total flavonoids of Dracaena cochinchinensis.

[0042] 【Properties】This product is white needle crystal.

[0043] 【7-Hydroxy-4'-methoxyflavan spectral data】

[0044] Molecular formula: C 16 h 16 o 3 White needle crystal, mp:134-135℃; UV(MeOH)λ max nm:209.8,224,281.9;

[0045] IR(KBr)cm -1 :3461,3380,1609,1592,1340,1153,1072,998,839; EI-MSm / z:256[M] + ,150,137,120,107,91,78,65;

[0046] 1 H-NMR (500MHz, CDCl 3 )δ: 7.33 (dd, 2H, J = 8.5, 2.6Hz, H-2', 6'), 7.01 (d, 1H, J = 8.2Hz, H-5), 6.87 (dd, 2H, J = 8.6 ,2.6Hz,H-3',5'),6.52(d,1H,J=2.6Hz,H-8),6.50(dd,1H,J=8.2,2.6Hz,H-6,8),5.00 (dd,1H,J=10.3,2.4Hz,H-2),3.79(s,3H,H-OCH 3 ),2.94(m,1H,H-4...

Embodiment 2

[0052] Embodiment 2 Pterostilbene chemical reference substance research

[0053] Pterostilbene, its molecular formula is:

[0054]

[0055] 【Source】Dracaenacochinchinensis (Lour.) S.C.Chen, a plant of the Liliaceae family, extracts effective parts from lipid-containing wood—total flavonoids of Dracaena cochinchinensis.

[0056] 【Properties】This product is white needle crystal.

[0057] 【Spectral data of rosewood stilbene】

[0058] Molecular formula: C 16 h 16 0 3 , white needle crystal, mp: 93~94℃; UV(MeOH)λ max nm:218,235,306;IR(KBr)cm -1 :3224,2933,2830,1589,1511,1454,1421,1359,1295,1238,1203,1182,1151,1062,964; EI-MSm / z:256(M + ); 1 H-NMR (500MHz, CDCl 3 )δ:7.39(m,2H,H-2',6'),7.02(d,1H,J=16.2Hz,β-H),6.89(d,1H,J=16.2Hz,ɑ-H), 6.82(m,2H,H-2,6),6.65(d,2H,J=2.3Hz,H-3',5'),6.38(t,1H,J=2.2Hz,H-4),3.83 (s,6H,H-OCH 3 ). 13 C-NMR (125MHz, CDCl 3 )δ:106.95(C-3,5),155.36(C-4'),139.68(C-1),130.16(C-1'),128.70(C-β),128.04(C-2',6 '),126.65(C-ɑ),115.65(C-3',5'),104.41(C-2...

Embodiment 3

[0064] Example 3: Determination of the content of 7-hydroxy-4'-methoxyflavan and pterostilbene in the total flavonoids of xiphophyllin

[0065] 1 Instruments, medicines and reagents

[0066] 1.1 Instrument

[0067] Waters2695 high performance liquid chromatography, waters996 diode matrix detector, Empower chromatography workstation.

[0068] 1.2 Drugs and reagents

[0069] Pterostilbene, 7-hydroxyl-4'-methoxyflavan standard substance (purity is greater than 98.0%) detected by embodiment 1 and embodiment 2; total flavonoids of Dracaena japonica; methanol and acetonitrile are chromatographically pure; water Ultrapure water; phosphoric acid is analytically pure.

[0070] The preparation method of the total flavonoids of Dracaena saponifera is as follows:

[0071] (1) get Dracaena saponifera, be ground into coarse powder;

[0072] (2) adding 75% ethanol containing 0.5‰ sodium hydroxide to reflux extraction twice, adding 10 times the amount each time, 1 hour each time, filteri...

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Abstract

The invention relates to a dracaena cochinchinensis quality detection method. The detection method comprises a step of measuring the content of 7-hydroxyl-4'-methoxylflavane and / or pterostilbene in dracaena cochinchinensis extract (dracaena cochinchinensis general flavone) through a wavelength conversion HPLC method, wherein the content of 7-hydroxyl-4'-methoxylflavane in the dracaena cochinchinensis general flavone is not less than 0.10 wt%, and / or the content of pterostilbene in the dracaena cochinchinensis general flavone is not less than 0.20 wt%. Through the optimization of the chromatogram conditions and application of the wavelength conversion HPLC method, two low polarity effective components in dracaena cochinchinensis can be simultaneously measured, so the method has a very important meaning in the overall quality control of medicinal materials and extracts.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a quality detection method of total flavonoids of Dracaena glabrata. Background technique [0002] Sword leaf Dracaena is recorded in the first volume of "Quality Standards of Zhuang Medicine of Guangxi Zhuang Autonomous Region". , Congestion and pain, stagnation of Qi and blood in women, traumatic bleeding and abscesses that persist for a long time. It is often used as a raw material for extracting dracaena, from which the inventors extracted and refined the effective part-total flavonoids of Dracaena glabra, which is used for treating angina pectoris of coronary heart disease. Modern studies have shown that it mainly contains flavonoids, stilbenes, sterols, saponins and other compounds, which have antibacterial, anti-inflammatory, antioxidant activities, analgesic, hemostatic, blood circulation and blood stasis and other pharmacological effects. At present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 邹节明周艳林
Owner GUILIN SANJIN PHARMACEUTICALS CO LTD
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