'Zhengqi' tablet fingerprint detection method and application

A technology of fingerprint spectrum and detection method, which is applied in the field of detection of traditional Chinese medicine components

Pending Publication Date: 2018-10-09
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Qualitative research on the Zhengqi

Method used

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  • 'Zhengqi' tablet fingerprint detection method and application
  • 'Zhengqi' tablet fingerprint detection method and application
  • 'Zhengqi' tablet fingerprint detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] 1. Sample pretreatment

[0126] Preparation of the test solution: Accurately weigh 2g of Zhengqi tablet powder, place it in a 50mL EP tube, accurately add 15-25ml of ethanol aqueous solution with a concentration of 50-100% by volume, weigh it, seal it with an ultrasonic wave Ultrasonic extraction for 20-40min, weigh again, use 50-100% ethanol aqueous solution to reduce the lost weight, centrifuge at 2000-4000r / min for 15-25min, take the supernatant, and filter it with a filter membrane with a pore size of 0.22μm , that is, the test solution.

[0127] Preparation of the reference substance solution: Accurately weigh the reference substances of cressin lactone, dehydrocressine lactone, atractylodes, liquiritin, hesperidin, magnolol, and honokiol in volumetric flasks. Serial dilutions. That is, first add 50-100% ethanol aqueous solution to dissolve and shake to constant volume in a volumetric flask to obtain a reference substance stock solution. The concentration of each...

Embodiment 2

[0145] 1. Sample pretreatment

[0146] Preparation of the test solution: Accurately weigh 2g of Zhengqi tablet powder, place it in a 50mL EP tube, accurately add 20ml of ethanol aqueous solution with a concentration of 70% by volume, weigh it, seal it and extract it by ultrasonic wave for 30min , weighed again, with the volume percentage concentration being 70% ethanol aqueous solution to lose the weight, centrifugal 20min under 3000r / min, get the supernatant, be that the filter membrane filtration of 0.22 μ m is obtained the need testing solution with aperture.

[0147] Preparation of the reference substance solution: Accurately weigh coaxolide, dehydrocoyolide, atractylodesin, liquiritin, hesperidin, magnolol, and honokiol, respectively, and perform stepwise dilution. That is, first place each 5 mg of the reference substance in a 10 mL volumetric flask, add volume percent concentration of 100% absolute ethanol to dissolve and shake up at a constant volume in the volumetric f...

Embodiment 3

[0181] Adopt the detection method of Zhengqi sheet fingerprint spectrum set up in above-mentioned embodiment 2 to detect 10 batches of Zhengqi sheet, obtain the fingerprint collection of test solution and the fingerprint collection of reference substance solution, the batch number of 10 batches of Zhengqi sheet is shown in Table 4 . The obtained fingerprint data of the test product was imported into the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" 2009 version software issued by the National Pharmacopoeia Committee, and compared with the standard fingerprint of Zhengqi Tablet obtained under the same fingerprint detection conditions, the similarity was compared. Taking the similarity ≥ 0.90 between the fingerprint spectrum of Zhengqi Tablet and the standard fingerprint spectrum of Zhengqi Tablet as the standard, and taking the S1 sample in Table 4 as the reference spectrum, it is determined that the standard fingerprint spectrum of Zhengqi Tablet i...

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Abstract

A 'Zhengqi' tablet fingerprint detection method is provided. The method includes 1) preparation of a solution of a sample to be tested, 2) preparation of a contrast solution and 3) measurement. The invention further provides application of the 'Zhengqi' tablet fingerprint in 'Zhengqi' tablet component quality detection and a quality detection method thereof. The invention further provides a fingerprint screening method for a plurality of medicine materials in the 'Zhengqi' tablets. Through the 'Zhengqi' tablet fingerprint detection method and application, a high-performance liquid chromatogramfingerprint of the 'Zhengqi' tablets is established, chromatographic peaks of medicine materials in the 'Zhengqi' tablets are assigned and confirmed, effective monitoring can be performed from the source of each raw material, raw material quality is strictly controlled, and relatively stable contents of effective components in the components of the 'Zhengqi' tablets are ensured, thus ensuring safety and effectiveness of clinical medication.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and relates to a detection method of fingerprints of Zhengqi tablets and an application thereof, in particular to a detection method of fingerprints of Zhengqi tablets and its application in quality detection. Background technique [0002] Zhengqi, also known as Huoxiang Zhengqi, is a traditional Chinese patent medicine, mainly used to treat colds, headaches, chest tightness, vomiting, diarrhea and abdominal distension, etc. It can also harmonize the stomach and stop vomiting. It is mostly used for nausea and vomiting caused by excessive dampness and turbidity. [0003] Although Zhengqi Tablet reveals the composition of the medicinal materials in it, its chemical composition is extremely complex. It is difficult to fully reflect the material basis and the integrity and complexity of the chemical composition group of the traditional Chinese medicine pre...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 沈丹萍向阳詹常森姜鹏
Owner HEHUANG PHARMA SHANGHAI
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