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Tissue culture and rapid propagation method for dracaena cochinchinensis

A technology of dragon blood tree and tissue culture, applied in the field of plant reproduction, can solve the problems of difficulty in realizing large-scale cultivation, limited number of seedlings, low reproduction rate, etc., and achieves improvement of seedling quality, reduction of cost consumption and cost reduction. Effect

Active Publication Date: 2015-04-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of seedling propagation, Dracaena can be propagated by seeds, cuttings or tillers, but the propagation rate of this conventional method is low, the time required is long, the number of seedlings obtained is very limited, and it is difficult to realize large-scale cultivation in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An example of the method for rapid propagation of the tissue culture of Dracaena in the present invention includes the following steps:

[0024] (1) Selection and disinfection of explants: Take dracaena seeds as explants, soak them in a 2% detergent solution for 5 minutes, rinse with linear tap water for 15-30 minutes, and add 2-3 drops of Tween. -20 100 ml 0.1% mercury disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface moisture with sterile filter paper to obtain explants, wherein the sterile water is autoclaved distilled water;

[0025] (2) Seed germination to obtain sterile test tube seedlings: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500 lux, and light time of 12-14 hours / day Cultivate for 30 days under the conditions of, and obtain sterile test tube seedlings after germination. MS medium contains 1.0 mg / L 6-benzyl adenine 6-BA, 0.2 mg / L indole ac...

Embodiment 2

[0031] Another example of the tissue culture rapid propagation method of Dracaena vulgaris according to the present invention includes the following steps:

[0032] (1) Selection and disinfection of explants: Take dracaena seeds as explants, soak them in a 2% detergent solution for 5 minutes, rinse with linear tap water for 15-30 minutes, and add 2-3 drops of Tween. -20 100 ml 0.1% mercury disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface moisture with sterile filter paper to obtain explants, wherein the sterile water is autoclaved distilled water;

[0033] (2) Seed germination to obtain sterile test tube seedlings: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500 lux, and light time of 12-14 hours / day Cultivate for 30 days under the conditions of, and obtain sterile test tube seedlings after germination. MS medium contains 1.0 mg / L 6-benzyl adenine 6-BA, 0.2 m...

Embodiment 3

[0039] Another example of the tissue culture rapid propagation method of Dracaena vulgaris according to the present invention includes the following steps:

[0040] (1) Selection and disinfection of explants: Take dracaena seeds as explants, soak them in a 2% detergent solution for 5 minutes, rinse with linear tap water for 15-30 minutes, and add 2-3 drops of Tween. -20 100 ml 0.1% mercury disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface moisture with sterile filter paper to obtain explants, wherein the sterile water is autoclaved distilled water;

[0041] (2) Seed germination to obtain sterile test tube seedlings: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500 lux, and light time of 12-14 hours / day Cultivate for 30 days under the conditions of, and obtain sterile test tube seedlings after germination. MS medium contains 1.0 mg / L 6-benzyl adenine 6-BA, 0.2 m...

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Abstract

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.

Description

Technical field [0001] The invention relates to a plant propagation method, in particular to a tissue culture rapid propagation method of dracaena scoparia. Background technique [0002] Sword-leaf Dracaena, also known as Millennium Wood, Dragon Blood Tree, and Cochin Dracaena, is an endangered species. It is a national second-level protected plant. It was classified as a vulnerable species by the International Federation for the Conservation of Nature and Natural Resources in 2003. It is listed as a red list and is designated as an endangered species in the Red Book of Cape Verde. It is currently recognized as the base source plant of domestic dragon's blood. [0003] Dragon's blood is a valuable traditional Chinese medicine, which has been used in my country for more than 1500 years. According to the "Compendium of Materia Medica", Dragon's Blood is warm, calm, sweet, salty, non-toxic, enters the blood, and returns to the lung, spleen and kidneys. It is known as the holy medici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韦坤华李林轩韦莹缪剑华李翠农东新黄雪彦王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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