Idesia leaf tissue culture method

A technology of tissue culture and sycamore, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low proliferation coefficient, many explants, and low reproductive efficiency, and achieve high proliferation coefficient and rooting rate. The effect of high reproduction efficiency and fast reproduction speed

Active Publication Date: 2016-12-21
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reproduction technology, the method of tissue culture has been reported, but in the report, the buds of the young shoots of the current year are used as the buds of the explants to carry out tissue culture and propagation, and many explants are used, and the proliferation coefficient is on the low side. low reproductive efficiency

Method used

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  • Idesia leaf tissue culture method
  • Idesia leaf tissue culture method
  • Idesia leaf tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The basic medium formula used in the in vitro culture of the leaves of Jacarna japonica is as table 1, table 2

[0021] Containing substance type and quality (inducing callus culture) in every liter of basic medium in table 1

[0022]

[0023]

[0024] The type and quality of substances contained in each liter of basic medium in Table 2 (differentiation and proliferation, strong seedling and rooting medium)

[0025]

[0026]

[0027] Callus culture: per liter of basic medium (Table 1) + ZT 0.2mg + BA0.5mg + NAA0.1mg + 2,4-D0.5mg;

[0028] Differentiation and proliferation culture: basic medium per liter (Table 2) + ZT 1.0mg + BA1.0mg + NAA0.08mg;

[0029] Strong seedling culture: per liter of basic medium (Table 2) + ZT 0.5mg + BA0.5mg + NAA0.1mg;

[0030] Rooting culture: per liter of basic medium (Table 2)+NAA0.3mg+IAA0.8mg+VC50mg.

[0031] Inject the above-mentioned medium into the Erlenmeyer flask, and sterilize it under high temperature and high pres...

Embodiment 2

[0039] This embodiment is operated according to the steps of Example 1, except that the weight ratio of the medium and the raw material components are different. The basic medium formula used in this embodiment is as shown in Table 3 and Table 4. The proliferation and proliferation of strong seedlings cultivated for 35 days The coefficient is 8.6, the height is 4.7cm, and the rooting rate is 94.3%.

[0040] Table 3 Contains substance species and quality in every liter of basic medium (induced callus culture)

[0041]

[0042]

[0043] Table 4 Contains the type and quality of substances per liter of basic medium (differentiation and proliferation, strong seedling and rooting medium)

[0044]

[0045]

[0046] Callus culture: basic medium per liter (Table 3) + ZT0.8mg + BA 1.0mg + NAA 0.2mg + 2,4-D1.0mg;

[0047] Differentiation and proliferation medium: basic medium per liter (Table 4) + ZT 1.5mg + BA 1.5mg + NAA 0.1mg;

[0048] Strong seedling culture: basic medi...

Embodiment 3

[0051] Same as Example 1, the difference is that between the step (2) callus induction culture and the step (3) differentiation and proliferation culture, it also includes soaking the cultured callus in the solution for 2 days, in the solution The ingredients are: pyridoxine 5mg / L+ glycine 1mg / L+ sucrose 8g / L.

[0052] This method has a multiplication coefficient of 10.6 and a rooting rate of 98.9%.

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Abstract

The invention discloses an idesia leaf tissue culture method, which comprises the following steps of (1) explant selection and treatment; (2) callus induction and culture; (3) differentiation and proliferation culture; (4) strong seedling culture; (5) rooting culture; (6) transplant. Compared with the prior art, the idesia leaf tissue culture method provided by the invention has the advantages that a large number of high-quality seedlings are produced within a short period, less explants are used, the reproduction speed is fast, the growth coefficient and the rooting percentage are high, and the reproduction rate is high.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant tissue culture, in particular to a tissue culture method in which the leaves of Jacarna japonica are used as explants. Background technique [0002] Mountain Tongzi (Idesia polycarpa Maxim.) is also known as chair, water winter melon, water winter tung, chair tree, chair tung, and bucket frost red. Deciduous trees of the family Typhoidaceae and Jacarnia, the bark is light gray and not cracked; the branchlets are cylindrical, thin and brittle, yellow-brown, with obvious lenticels, the side branches are longer than the top branches in winter, the branches are flat and nearly whorled, The crown is oblong, the branches of the current year are purple-green, with light yellow long hairs; the winter buds have light brown hairs, the flowers are unisexual, dioecious or polygamous, yellow-green, fragrant, petals absent, arranged in terminal drooping Inflorescence panicles, peduncles are sparsely p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 邱国金蒋泽平史云光姚振宇
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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