Litsea cubeba extracting solution and preparation method and application thereof

A technique for extracting liquid of A. japonica is applied in the field of plant tissue extracting liquid and culturing, which can solve the problems of restricting C. japonica, low induction rate of clump buds, low value-added coefficient, etc., so as to avoid loss of effective components and improve the induction rate. and value-added coefficient, the effect of improving efficiency

Active Publication Date: 2019-10-11
HUNAN NASTAR FLAVOURS BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the tissue culture of Litsea Cubeba, the stems with buds are mostly used as explant materials for tissue culture, and the in vitro rapid propagation is carried out through the induction of clustered buds. However, the existing methods have low rate of clustered bud induction and low value-added coefficient. shortcoming, which seriously restricts the efficiency of Litsea cubeba rapid propagation in vitro

Method used

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  • Litsea cubeba extracting solution and preparation method and application thereof
  • Litsea cubeba extracting solution and preparation method and application thereof
  • Litsea cubeba extracting solution and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The preparation of embodiment 1 litsea cubeba extract

[0029] Take 4g of 3cm-long young and elongated branches germinated from the adventitious buds at the base of the main trunk of Litsea cubeba, grind them into powder with liquid nitrogen, collect them in a 50ml centrifuge tube, add 25ml of distilled water and 0.5g of vitamin C, and keep them in a water bath at 40°C for 3 hours, and then centrifuged at 8000rpm / min for 3 minutes, and the supernatant was drawn up to 50ml to obtain Litsea Cubeba Extract A, which was placed in a refrigerator at 4°C for later use.

Embodiment 2

[0030] The preparation of embodiment 2 litsea cubeba extract

[0031] Take 8g of 5cm-long young and elongated branches germinated from the adventitious buds at the base of the main trunk of Litsea cubeba, grind them into powder with liquid nitrogen, collect them in a 50ml centrifuge tube, add 30ml of distilled water and 1g of polyvinylpyrrolidone (PVP), and place them in a water bath at 40°C Incubate in medium for 4 hours, then centrifuge at 10000rpm / min for 5 minutes, absorb the supernatant and make up to 50ml to prepare Litsea Cubeba Extract B, and put it in a refrigerator at 4°C for later use.

Embodiment 3

[0032] The preparation of embodiment 3 litsea cubeba extract

[0033] Get 6g of 4cm-long young and elongated shoots germinated from the adventitious buds at the trunk base of Litsea cubeba, grind them into powder with liquid nitrogen and collect them in a 50ml centrifuge tube, add 27ml of distilled water, 1g of vitamin C and 1.5g of polyvinylpyrrolidone (PVP), place Incubate in a water bath at 40°C for 4 hours, then centrifuge at 10,000rpm / min for 4 minutes, absorb the supernatant and make up to 50ml to obtain Litsea Cubeba Extract C, and store it in a refrigerator at 4°C for later use.

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Abstract

The invention discloses a litsea cubeba extracting solution and a preparation method and application thereof. The litsea cubeba extracting solution is prepared from litsea cubeba, water and an additive, wherein the additive includes vitamin C and / or polyvinylpyrrolidone, and the mass ratio of the litsea cubeba to the additive is (4-8):(0.5-2.5). The preparation method comprises the steps that thelitsea cubeba, by mass part, is ground into powder to obtain litsea cubeba powder; the litsea cubeba powder is mixed with, by mass part, the additive and part of water to obtain a mixed solution; after the mixed solution is subjected to heat preservation and centrifugalization, supernatant liquid is obtained and placed into a container, the volume is set by using water to a scale, and then the litsea cubeba extracting solution is obtained. The litsea cubeba extracting solution is used in litsea cubeba tissue culture, the inductivity and the growth coefficient of litsea cubeba cluster buds canbe obviously improved, therefore, the in-vitro rapid propagation efficiency of the litsea cubeba is improved, and the development of the litsea cubeba industry is promoted.

Description

technical field [0001] The invention relates to the field of plant tissue extract and cultivation, more specifically, to a litsea cubeba extract and its preparation method and application. Background technique [0002] Litsea cubeba (Litsea cubeba) is a unique industrial raw material and spice tree species in southern my country. The essential oil extracted from its fruit has a high content of citral and is widely used in medicine, food, cosmetics and other fields. The establishment of the tissue culture and rapid propagation system of Litsea cubeba can not only promote the development of Litsea cubeba industry, but also lay the foundation for the basic research of Litsea cubeba. At present, in the tissue culture of Litsea Cubeba, the stems with buds are mostly used as explant materials for tissue culture, and the in vitro rapid propagation is carried out through the induction of clustered buds. However, the existing methods have low rate of clustered bud induction and low v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 陈昊张付豪
Owner HUNAN NASTAR FLAVOURS BIOTECHNOLOGY CO LTD
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