Rapid propagation method for holcoglossum flavescens

A short-distance and rapid technology for grove tongue, applied in the field of plant tissue culture, can solve the problems of difficult multiplication, low coefficient, difficulty in meeting protection and development requirements, etc., achieve high transplanting survival rate, simple preparation, and solve resource protection problem effect

Active Publication Date: 2015-10-28
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the plants of the genus Grogonia still adopt the traditional orchid ramet technique, that is, the steps of raising seedlings, washing roots, branching, and disinfecting, etc., the multiplication will be difficult and the coefficient will be low, which will form a barrier for the effective propagation of the short-distance Grogula , it is difficult to meet the protection and development requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine. That is, from May to June each year, the flowering season of the short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized. After the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, soak with 10% sodium hypochlorite for 10 minutes, and final...

Embodiment 2

[0026] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine. That is, from May to June each year, the flowering season of the short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized. After the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, soak with 10% sodium hypochlorite for 10 minutes, and final...

Embodiment 3

[0034] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine. That is, from May to June each year, the flowering season of the short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized. After the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, soak with 10% sodium hypochlorite for 10 minutes, and final...

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Abstract

The invention discloses a rapid propagation method for holcoglossum flavescens. Rapid propagation of holcoglossum flavescens is completed through the steps of seed germination, induction of protocorm, differentiation culture, strong seedling culture, rooting culture, acclimatization and transplant and the like. Compared with a traditional division method, the growth coefficient of holcoglossum flavescens is improved by more than 5 times, the planting percent reaches 95%, the survival rate of transplanting reaches more than 85%, meanwhile, the method has the advantages of being large in increment coefficient, strong in tillering capacity, high in growth speed, strong in plant, low in browning ratio, high in survival rate of transplanting, and low in cost, culture medium is easy to prepare, and the method can be applied to large scale industrial production.

Description

Technical field [0001] The invention relates to a method for propagation of rare and endangered orchid plants, in particular to a method for rapid propagation of short-distance trough hyacinth, which belongs to the technical field of plant tissue culture. Background technique [0002] Short grooved tongue ( Holcoglossumflavescens(Schltr.)Z.H.Tsi ) Is Orchid ( Orchidaceae ) Groophyllum (Holcoglossum )plant. Produced in northern Fujian (Mount Wuyi, Jianyang), southwestern Hubei (Lichuan), southwestern Sichuan (Panzhihua), and northern Yunnan (Binchuan, Yongsheng). Born on the trunk of an evergreen broad-leaved forest at an altitude of 1200 to 2000 meters. The short-span grooved tongue orchid is a treasure in the orchid family, its shape is elegant, the flower color is gorgeous, and it exudes fragrance. It can be placed in shallow pots with fern roots and other attached plants, or hung, which is very elegant. There are a total of 12 species of the genus Cercophyllum, distribut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李涵曹桦李慧敏蒋亚莲阮继伟黎霞赵培飞杨维杨春梅
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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