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Method for rapid propagation of virus-free artemisia seleirgensis seedlings

A technology for detoxifying seedlings and Artemisia annua, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as the degeneration of varieties with poison, and achieve the effects of fast reproduction, rapid reproduction, and large-scale production.

Active Publication Date: 2017-05-10
灌云县现代农业产业园区管理委员会 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for rapid propagation of Artemisia annua virus-free seedlings that can efficiently detoxify Artemisia annua, restore the variety, rapid propagation, and realize the large-scale supply of Artemisia annua seedlings, so as to solve the problem of long-term asexual reproduction in production. The problem of virus-infected and degraded varieties caused by the retention of seedlings provides high-quality virus-free seedlings for the production of Artemisia annua, improving the yield and quality of Artemisia annua

Method used

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  • Method for rapid propagation of virus-free artemisia seleirgensis seedlings
  • Method for rapid propagation of virus-free artemisia seleirgensis seedlings
  • Method for rapid propagation of virus-free artemisia seleirgensis seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Test medium sterilization and culture conditions

[0024] Before autoclaving, adjust the pH value of the medium to 5.8, and keep it at 121°C for 20 minutes for sterilization. The temperature of the culture room is 25°C, the light intensity is 1300lx, and the light is 12h / d every day. The mass fraction of agar in MS medium is 7g / L, and the mass fraction of sucrose is 30g / L.

[0025] 2. Handling of sterile materials

[0026] Select healthy Kunming Artemisia annua plants, cut 1-1.5 cm healthy sectioned stems from the upper part, wash them in running water for 10 minutes, put them on a super-clean workbench, and wash the Artemisia annua stems with 75 % alcohol for 30 seconds, then sterilized with 84 disinfectant for 5 minutes, rinsed with sterile water for 5 times, and then inoculated in MS medium.

[0027] 3. Axillary bud induction

[0028] (1) Sterilized stems with joints were inoculated with different concentrations of 6-BA and 0.2 mg·l -1 In the NAA-combined med...

Embodiment 2

[0045] 1. Test medium sterilization and culture conditions

[0046] Before autoclaving, adjust the pH value of the medium to 5.8, and keep it at 121°C for 20 minutes for sterilization. The temperature of the culture room is 25°C, the light intensity is 1300lx, and the light is 12h / d every day. The mass fraction of agar in MS medium is 7g / L, and the mass fraction of sucrose is 30g / L.

[0047] 2. Handling of sterile materials

[0048]Select healthy Kunming Artemisia annua plants, cut 1-1.5cm healthy sectioned stems from the upper part, wash them in running water for 10 minutes, put them on a super-clean workbench, wash the Artemisia annua stems with 75 % alcohol for 30 seconds, then sterilized with 84 disinfectant for 5 minutes, rinsed with sterile water for 5 times, and then inoculated in MS medium.

[0049] 3. Axillary bud induction

[0050] Sterilized stems with joints were inoculated in MS+6-BA1.0mg·l -1 +NAA0.4mg·l -1 in the culture medium.

[0051] 4. Virus-free cul...

Embodiment 3

[0057] 1. Test medium sterilization and culture conditions

[0058] Before autoclaving, adjust the pH value of the medium to 5.8, and keep it at 121°C for 20 minutes for sterilization. The temperature of the culture room is 25°C, the light intensity is 1300lx, and the light is 12h / d every day. The mass fraction of agar in MS medium is 7g / L, and the mass fraction of sucrose is 30g / L.

[0059] 2. Handling of sterile materials

[0060] Select healthy Kunming Artemisia annua plants, cut 1-1.5cm healthy sectioned stems from the upper part, wash them in running water for 20 minutes, put them on a super-clean workbench, and wash the Artemisia annua stems with 75 % alcohol soaked for 60s, then sterilized with 84 disinfectant for 10min, rinsed with sterile water for 5 times, and then inoculated in MS medium.

[0061] 3. Axillary bud induction

[0062] Sterilized stems with joints were inoculated in MS+6-BA1.0mg·l -1 +NAA0.4mg·l -1 in the culture medium.

[0063] 4. Virus-free cu...

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Abstract

The invention discloses a method for rapid propagation of virus-free artemisia seleirgensis seedlings. Middle and upper section stems of healthy artemisia seleirgensis plants having bright variety characteristics are selected to serve as explants, and finally complete virus-free artemisia seleirgensis seedlings are formed through axillary bud induction, detoxification culture, rooting culture and transplanting and field planting. The virus-free artemisia seleirgensis seedlings have a quick propagation speed, good virus-free effects, good growth vigor and good genetic stability, the whole process from explant inoculation to formation of transplantable tube seedlings only needs about 90 d, the growth coefficient can reach 20, the problems of severe seedling viruses and degradation of yield and quality caused medium and long term asexual reproduction in artemisia seleirgensis growth are solved, the virus-free artemisia seleirgensis seedlings propagate quickly, and large-scale production of the virus-free seedlings is met.

Description

technical field [0001] The invention relates to a rapid propagation method for virus-free seedlings of Artemisia annua, in particular to a rapid propagation method for obtaining virus-free test-tube seedlings through tissue culture of artemisia artemisiae with jointed stem sections under in vitro conditions, and belongs to the field of biotechnology. Background technique [0002] Artemisia annua is a perennial herb of the genus Artemisia in the family Asteraceae. It is rich in nutrition, crisp and tender, and has the effects of cooling, calming liver fire, expelling rheumatism, anti-inflammatory, and antitussive. In addition, it is good for lowering blood pressure, blood fat, and relieving cardiovascular diseases. It is a typical health vegetable with broad development prospects. In current production, Artemisia annua is mainly produced through vegetative propagation of stem cuttings and seedlings are preserved, and the seedlings are mainly self-propagated by the masses for ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 韩晓勇王超马丙四周天豹杨海莲曹巍邱远郭文琦张培通李春宏殷剑美王立
Owner 灌云县现代农业产业园区管理委员会
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