Detoxification and rapid propagation method for edible lily

An edible lily, fast technology, applied in horticultural methods, botanical equipment and methods, applications and other directions, can solve the problems of difficult chemical control, poisoning and the like

Active Publication Date: 2016-08-10
HUNAN VEGETABLE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the lily is infected with the virus, it will carry the virus for life, and it is difficult to directly and effectively control it with chemical agents or biological agents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] This embodiment includes the following steps:

[0017] (1) Selection and treatment of explants: choose lily bulbils as explants, first wash with water to remove soil, then put them into a wide-mouth container, cover with a wet towel, and then place them in a 38±1°C incubator. Medium heat treatment for 15 days, keeping the relative humidity at 100%;

[0018] (2) Sterilization of explants and stripping of growth points: wash the lily bulbils after heat treatment with detergent in water, after cleaning the surface mucus, soak them in 75% alcohol for 25 seconds, and then After sterilizing with 0.15% mercuric chloride for 15 minutes, wash it with sterile water for 4 times, wash off the residual liquid, and set aside;

[0019] Place the sterilized lily bulbils under a stereomicroscope, first cut off the larger scales around them with a scalpel, and then pick out 0.4-0.6mm-sized stem tips with a dissecting needle, and inoculate them in the starting medium;

[0020] (3) Start...

Embodiment 2

[0025] This embodiment includes the following steps:

[0026] (1) Selection and treatment of explants: Lilium bulbils were selected as explants, washed with water to remove soil, then put into a wide-mouth container, covered with a wet towel, and then placed in a 36±1°C incubator. Medium heat treatment for 12 days, keep the relative humidity at 85±1%;

[0027] (2) Sterilization of explants and stripping of growth points: wash the lily bulbils after heat treatment with detergent in water, after cleaning the surface mucus, soak them in 70% alcohol for 20 seconds, and then Sterilize with 0.12% mercuric chloride for 12 minutes, then wash 4 times with sterile water, wash the residual liquid, and set aside;

[0028] Place the sterilized lily bulbils under a stereomicroscope, first cut off the larger scales around them with a scalpel, and then pick out 0.4-0.6mm-sized stem tips with a dissecting needle, and inoculate them in the starting medium;

[0029] (3) Start-up culture: Start...

Embodiment 3

[0034] This embodiment includes the following steps:

[0035] (1) Selection and treatment of explants: choose lily bulbils as explants, first wash with water to remove soil, then put them into a wide-mouth container, cover with a wet towel, and then place them in a 37±1°C incubator. Medium heat treatment for 18 days, keep the relative humidity at 90±1%;

[0036] (2) Sterilization of explants and stripping of growth points: wash the lily bulbils after heat treatment with detergent in water, after cleaning the surface mucus, soak them in 75% alcohol for 30 seconds, and then Sterilize with 0.12% mercuric chloride for 12 minutes, then wash 4 times with sterile water, wash the residual liquid, and set aside;

[0037]Place the sterilized lily bulbils under a stereomicroscope, first cut off the larger scales around them with a scalpel, and then pick out 0.4-0.6mm-sized stem tips with a dissecting needle, and inoculate them in the starting medium;

[0038] (3) Start-up culture: Star...

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PUM

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Abstract

The invention discloses a detoxification and rapid propagation method for edible lily. The detoxification and rapid propagation method comprises the following steps: (1) selecting and treating explant; (2) sterilizing the explant, and picking off growing points; (3) starting culture; (4) performing RT-PCR virus detection and cluster bud induction culture; (5) performing bulb induction and rooting culture. By adopting the detoxification and rapid propagation method, main viruses in the lily can be removed for 100%, particularly, multiple times of tests show that the viruses in infected lily explant can be successfully removed, and moreover the survival rate is greater than 85%; in addition, the growth period of the lily can be shortened while a relatively high growth coefficient is obtained, a great amount of detoxified lily seedlings can be cultured within a short time, and thus the production requirements can be met.

Description

technical field [0001] The invention relates to a new technology for high-efficiency detoxification and rapid propagation of lily, in particular to the tissue culture method with extremely high efficiency of detoxification and rapid propagation of lily lily. Background technique [0002] Lily lily is distributed in Hunan, Anhui, Jiangxi and other provinces (districts). Only in Longshan County, Hunan Province, 3400hm of lily lily, which is used for medicine and food, is planted all the year round. 2 , is one of the county's agricultural pillar industries. Because asexual reproduction is often used in lily production, a large number of viruses accumulate and viral diseases occur in plants, resulting in a sharp decline in yield and quality, which restricts the development of lily. [0003] The main virus diseases that harm lily include lily symptomless virus (LSV), lily rosette virus (LRV), cucumber mosaic virus (CMV) and so on. Once the lily is infected with the virus, it wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 周晓波向清平吴艺飞向永号丁茁荑张治国袁祖华李世树戴雄泽周仕兵刘峰
Owner HUNAN VEGETABLE RES INST
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