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Detoxifying tissue-culture rapid propagation method for fruit cane

A technology for tissue culture rapid propagation and fruit cane, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of unstable genetic traits, incomplete detoxification, difficult operation, etc. The effect of high detoxification rate and high reproduction coefficient

Pending Publication Date: 2017-12-22
GUANGDONG ZHANJIANG LAND RECLAMATION SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for rapid propagation of fruit cane detoxified tissue culture, which effectively combines warm water treatment and high-temperature germination in an artificial climate box to attenuate the cane buds, and uses tissue culture stem tip detoxification technology and Tissue culture and rapid propagation technology can obtain a large number of virus-free fruit cane tissue culture seedlings in a short period of time, which solves the problems of incomplete virus-free, difficult operation, unstable genetic traits, etc., and can effectively meet production needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 takes Yuedong No. 8 fruit cane as material, and adopts the following steps to realize the present invention:

[0023] 1) Selection of explants: Select pure, vigorous, disease-free plants with plump cane buds, take the mature and plump cane stems with middle cane buds, cut them into single bud segments, and wash them with tap water for later use;

[0024] 2) High-temperature attenuation treatment: Put the single bud section treated in step 1) into a 50°C constant temperature water tank for warm treatment for 2 hours, pick it up and dry it, and then plant it on the pre-sterilized coconut peat + peat soil substrate placed in an artificial climate box at 38-40°C for continuous high-temperature and constant-temperature germination for 8 days, during which time water was sprayed to keep the substrate moist, and the air was changed twice a day for 2 minutes each time; when the cane buds grew to 6cm high, they were taken out as The next step is tissue culture and i...

Embodiment 2

[0030] Embodiment 2 Taking Dongxuan No. 7 fruit cane as material, adopt the following steps to realize the present invention:

[0031] 1) Selection of explants: Select pure, vigorous, disease-free plants with plump cane buds, take the mature and plump cane stems with middle cane buds, cut them into single bud segments, and wash them with tap water for later use;

[0032] 2) High-temperature attenuation treatment: Put the single bud section treated in step 1) into a 50°C constant temperature water tank for warm treatment for 2 hours, pick it up and dry it, and then plant it on the pre-sterilized coconut peat + peat soil substrate placed in an artificial climate box at 38-40°C for 10 consecutive days at high temperature and constant temperature, during which time water was sprayed to keep the substrate moist, and the air was changed 3 times a day, each time for 1 minute; when the cane buds grew to 8 cm high, they were taken out as The next step is tissue culture and inoculation ...

Embodiment 3

[0038] Embodiment 3 takes Yuedong No. 8 fruit cane as material, and adopts the following steps to realize the present invention:

[0039] 1) Selection of explants: Select pure, vigorous, disease-free plants with plump cane buds, take the mature and plump cane stems with middle cane buds, cut them into single bud segments, and wash them with tap water for later use;

[0040] 2) High-temperature attenuation treatment: Put the single bud section treated in step 1) into a 50°C constant temperature water tank for warm treatment for 2 hours, pick it up and dry it, and then plant it on the pre-sterilized coconut peat + peat soil substrate placed in an artificial climate box at 38-40°C for 12 consecutive days at high temperature and constant temperature, during which time water was sprayed to keep the substrate moist, and the air was changed 3 times a day for 2 minutes each time; when the cane buds grew to a height of 10cm, they were taken out as The next step is tissue culture and in...

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Abstract

The invention discloses a detoxifying tissue-culture rapid propagation method for fruit cane. The method disclosed by the invention comprises the following steps: 1) selecting an explant; 2) detoxifying under high temperature; 3) detoxifying and inoculating stem tips and acquiring an aseptic seedling; 4) detecting pathogene; 5) performing subculture multiplication; 6) rooting and hardening seedlings; and 7) heeling in, transplanting and managing. According to the invention, the warm water treatment is effectively combined with the manual climatic box high-temperature pregermination, the cane buds are subjected to detoxifying treatment and the detoxifying rate is high; 3-5mm stem tips are selected for starting the inoculated culture and the survival rate is 90% or above; the culture process is started, the browning is effectively avoided and the yield of the sterile strain is increased in the manner of changing the manner of starting the culture medium and adding PVP into the culture medium; MET component is added into a rooting culture medium, so that quick rooting and more and regular roots rooting in the rooting process can be realized; low-concentration hormone is added into the culture medium in the tissue-culture rapid propagation process, the influence of the hormone on the tissue culture seedling is extremely reduced, the original nature of the variety is kept, the growth coefficient is 2-4 times and the purposes of purification, rejuvenation and rapid propagation can be achieved.

Description

technical field [0001] The invention relates to a method for rapid propagation of virus-free tissue culture of plants, in particular to a method for rapid propagation of virus-free tissue culture of fruit cane, and belongs to the technical field of virus-free tissue culture of sugarcane. Background technique [0002] At present, the variety of fruit cane planted in my country is single and the planting years are many. It is seriously infected with seedling diseases such as perennial dwarf disease and mosaic disease, and the seed quality is seriously degraded, which affects the yield and quality of fruit cane. The selection of detoxified healthy seedlings can effectively remove the pathogenic bacteria of seed-borne diseases such as dwarf disease and mosaic disease, and restore the excellent seed properties of fruit cane varieties. [0003] There are many ways to obtain virus-free healthy seedlings, such as treating seed stems with hot water, tissue culture for virus-free shoo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 田夏红揭进刘伟清李强有张曼其刘建荣沈万宽
Owner GUANGDONG ZHANJIANG LAND RECLAMATION SCI INST
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