Detoxifying tissue-culture rapid propagation method for fruit cane

Abstract

The invention discloses a detoxifying tissue-culture rapid propagation method for fruit cane. The method disclosed by the invention comprises the following steps: 1) selecting an explant; 2) detoxifying under high temperature; 3) detoxifying and inoculating stem tips and acquiring an aseptic seedling; 4) detecting pathogene; 5) performing subculture multiplication; 6) rooting and hardening seedlings; and 7) heeling in, transplanting and managing. According to the invention, the warm water treatment is effectively combined with the manual climatic box high-temperature pregermination, the cane buds are subjected to detoxifying treatment and the detoxifying rate is high; 3-5mm stem tips are selected for starting the inoculated culture and the survival rate is 90% or above; the culture process is started, the browning is effectively avoided and the yield of the sterile strain is increased in the manner of changing the manner of starting the culture medium and adding PVP into the culture medium; MET component is added into a rooting culture medium, so that quick rooting and more and regular roots rooting in the rooting process can be realized; low-concentration hormone is added into the culture medium in the tissue-culture rapid propagation process, the influence of the hormone on the tissue culture seedling is extremely reduced, the original nature of the variety is kept, the growth coefficient is 2-4 times and the purposes of purification, rejuvenation and rapid propagation can be achieved.

Description

technical field

[0001] The invention relates to a method for rapid propagation of virus-free tissue culture of plants, in particular to a method for rapid propagation of virus-free tissue culture of fruit cane, and belongs to the technical field of virus-free tissue culture of sugarcane. Background technique

[0002] At present, the variety of fruit cane planted in my country is single and the planting years are many. It is seriously infected with seedling diseases such as perennial dwarf disease and mosaic disease, and the seed quality is seriously degraded, which affects the yield and quality of fruit cane. The selection of detoxified healthy seedlings can effectively remove the pathogenic bacteria of seed-borne diseases such as dwarf disease and mosaic disease, and restore the excellent seed properties of fruit cane varieties.

[0003] There are many ways to obtain virus-free healthy seedlings, such as treating seed stems with hot water, tissue culture for virus-free shoo...